JPS63105098A - Enzyme-containing detergent composition - Google Patents

Abstract

(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention is directed to liquid or gel detergents for general household and commercial use containing enzymes, specifically for washing clothes,
The present invention relates to so-called household detergents for washing dishes, cleaning furniture or houses, shampoos, and detergents for body washing.

[Prior Art and its Problems] Adding enzymes to detergents provides favorable results for cleaning, such as improved cleaning efficiency, and various enzymes have been used or proposed.

For example, protease improves the cleaning efficiency by hydrolyzing protein components contained in human dirt (Takashi Ono, Bioindustry 3, 181. (1)
986) etc.). Lipase improves cleaning efficiency by converting triglycerides in human lipids, which are difficult to remove with surfactants, into diglycerides or monoglycerides (
H, Hr nagawa et al., J, Am.

Oil Chem, Soc, 621053-10
58 (1985) etc.).

Amyl 1 hydrolyzes the starch contained in food stains, making it easier to remove stains, and cellulase is effective in removing muddy stains, improving the color of cotton 'Qi's washed finish ( JP-A-59-1598, JP-A No. 57-1
No. 08199). Lysozyme is added in hopes of reducing the number of viable bacteria through bacteriolytic action and anti-inflammatory action during body washing.

These enzymes are generally relatively stable in a dry state, and when the enzyme is blended into a powdered detergent, the dry state is maintained, so the storage stability of the enzyme is good.

On the other hand, when an enzyme is in a dissolved state, its higher-order structure is easily changed and it is easily deactivated. Additionally, many of the anionic surfactants commonly used as main components of detergents deactivate enzymes in the presence of water. Therefore, incorporating enzymes into liquid detergents poses a problem in the storage stability of the enzymes, and various proposals have been made in the past as countermeasures to this problem.

For example, an alkyl ethoxylate with a large number of added ethylene oxides is used as a surfactant, and the pH is adjusted to 8 to 10.
5 and adding calcium salt (Japanese Patent Laid-open No. 58
-80397), a method of adding an antioxidant such as sodium ftLite and propylene glycol, etc. (JP-A No. 5
5-27378), a method of adding an alkoxylated alkylamine (JP-A-53-16012),
A method of adding a polyhydroxy compound and boric acid (JP-A-53-57209), a method of adding free calcium ions and a polyacid such as citric acid (JP-A-52-128)
904), a method of adding a nitrogen compound and a lower carboxylic acid salt (Japanese Patent Application Laid-Open No. 47-3733), etc. have been proposed.

However, despite these many proposals, the reality is that the storage stability of enzymes blended into liquid or gel detergents is not sufficient (C, A, 5 tar
ace, J, A)1. Oil Chem, Soc
,, 601025 (1983), etc.).

[Object of the Invention] Therefore, an object of the present invention is to provide a liquid or gel detergent composition containing an enzyme with good storage stability.

[Summary of the Invention] As a result of intensive studies to achieve the above object, the inventors of the present invention have found that although it is insoluble in commonly used liquid or gel detergents, when mixed with water together with detergents. An enzyme dissolved or dispersed in a water-containing polyhydroxy compound is coated with polyvinyl alcohol, which easily dissolves in water, to form microcapsules, and when these microcapsules are added to a detergent, the enzyme activity is stably maintained for a long period of time. The present invention was completed based on this discovery.

That is, the present invention is a detergent composition in which enzymes are coated with polyvinyl alcohol as microcapsules, and the microcapsules are uniformly dispersed in a liquid or gel detergent.

[Related Art to the Invention] The present invention is the first and unprecedented method of microcapsulating enzymes and blending them into liquid detergents. but,
Many proposals have been made regarding the microencapsulation of enzymes, including some aimed at detergent formulations, but all of these are aimed at powdered, dry detergents.

For example, a dispersion in which an enzyme and swellable particles coexist in a binder liquid is microencapsulated using a spray drying method, and the enzyme is protected in the dry state, but the enzyme is eluted in water. -42594tan), Enzyme granules for detergents whose coating layer is made of modified cellulose which is neutral to acidic and water-insoluble and alkaline and water-soluble (Special Publication No. 44471/1983), Enzyme and inorganic salt are water-soluble. Enzyme-containing capsules in which a solution coexisting in a binder solution is encapsulated by a spray-drying method to protect enzymes in a dry state and elute enzymes in water (Special Publication No. 50-225)
No. 06) etc.

All of these proposals are aimed at formulation into detergents in a dry state, and do not take into account formulation into systems where water is present, such as liquid detergents.

Microcapsules containing enzymes are those encapsulated with a water-insoluble and semi-permeable membrane for the purpose of immobilized enzymes (for example, Japanese Patent Publication No. 52-18271).
) or enteric capsules of enzymes developed for pharmaceutical use (e.g., JP-A-58-55413, JP-A-58-67)
No. 166), but it is difficult to use them for the same purpose as the present invention.

On the other hand, proposals have also been made to microcapsule mulberry agents other than enzymes and incorporate them into detergents.

For example, there is a composition in which a cationic agent is microencapsulated with dextrin or the like and blended into an anhydrous anionic shampoo (Japanese Patent Application Laid-open No. 50-3105), but in this case, the shampoo is anhydrous and the microcapsules are anhydrous. Otherwise it will collapse.

In addition, microcapsules coated with a special copolymer that is insoluble in alkaline water but soluble in neutral or acidic water have been proposed (Japanese Patent Laid-Open Nos. 61-28440 and 1983).
1-28441@), it is stated that if a laundry aid such as a fabric softener is applied to the core material, it can be used for detergent formulation. In this case as well, the fabric softener is intended to be released during rinsing due to the change in pl-1 of the washing liquid during washing, dehydration, rinsing, etc., which is different from the purpose of the present invention.

The microcapsules used in the present invention have a core material of a water-containing water-soluble substance, and have a very special property that they do not dissolve in a water-containing mixture such as liquid detergent, but dissolve in water.
unprecedented in the past.

[Section 14 of the Invention] Enzyme The enzyme applied to the detergent composition of the present invention is active under neutral or alkaline conditions and contributes to improving detergency, and is essential for the presence of detergent. It is desirable to have one that does not cause severe deactivation.

Examples of such enzymes include Bacillus licheniformis, Bacillus 5ubtilis,
Bacillus firmus, Bacillus SP, FERN, B
P-93 (JP 58-134990) Bacill
Protease produced by strains such as Bacillus alcalophilus; Bacillus 5ubtilis;
amylase produced by strains such as Bacillus licheniformis, Aspergillus oryzae;
)1ucor l1polyticus (Mucor lipolyticus) Candida cylindra
cea (Candida cylindrus), Pseudomonus n1troreducens
(Pseudomonas nitrolideucens), Pseudomonus fragi (Pseudomonas furan), Chromobacterium v
iscosum (Chromobacterium viscosum)
, Lipase produced by strains such as Rh1ZOpUS delelner (Rhizopus delelner) and lipase isolated from the pancreas of animals such as pigs; Bacillus Pa5teri, Trichoderma viride (1-
Lycoderma viride), eight supergillus ni
cellulase or hemicellulase produced by strains such as Aspergillus niger; chicken egg white +)'Ideme and Bacillus 5ub
Examples include lysozyme produced by strains such as B. tilis (Bacillus subtilis).

During microencapsulation of an enzyme support material, the enzyme is handled in a dissolved or dispersed state in the support material. Enzyme support materials are desirable for the integrity of the capsel coating and also serve to enhance the storage stability of the enzyme.

Desirable substances as supporting substances are water-soluble polyhydroxy compounds, that is, compounds having two or more carbon atoms and two or more hydroxyl groups.
~6 compounds and their low monomers.

Specific examples include ethylene glycol, propylene glycol, butanediol, butanetriol, glycerin, mannitol, sorbitol, glucose, fructose, diethylene glycol, 1-diethylene glycol, polypropylene glycol, dextrin, pectin, alginic acid, and the like.

Coating material The coating material for the microcapsules is one of the simplest structural types ('+) of the present invention. As a result of repeated various studies, it was discovered that a specific polyvinyl alcohol was optimal.

That is, the polyvinyl alcohol used as the microcapsule coating material in the present invention has (i) an average degree of polymerization of 200 to 4,000, preferably 500 to 2,50;
(ii) The saponification rate is 80% or more, preferably 87% or more.

If the degree of polymerization is too high, there will be problems in handling such as viscosity, and if the saponification rate is low, there will be problems in coating strength in liquid detergent.

In addition, for the purpose of improving the strength of the capsule, it may be effective to treat the capsule with a crosslinking agent such as boric acid or a boric acid derivative.

Alkaline earth metal salts and enzyme stabilizers In order to further improve the storage stability of enzymes, it is effective to add alkaline earth metal salts to enzyme support materials.

Although the enzyme stabilizing effect of the alkaline earth metal salt varies depending on each enzyme, it is desirable to add the alkaline earth metal salt at a ratio of 5 ppm or more to the enzyme bulk powder in the capsule.

If there is already a sufficient amount of calcium etc. in the enzyme bulk powder, there is no need to add it when making capsules, but if it is insufficient, add an alkaline earth metal salt that is water-soluble or at least partially soluble in water. For example, calcium acetate, calcium chloride, calcium nitrate, magnesium acetate, magnesium chloride, magnesium nitrate, etc. are added.

In order to stabilize the enzyme, in addition to the above-mentioned alkaline earth metal salts, antioxidants such as ascorbic acid and its derivatives, amino acids, polypeptides and their derivatives,
It is also effective to add substances that form protective colloids, such as gums, and these are applied as necessary.

Roughly stable to enzymes 1. 1-1 region, and in order to maintain the pH inside the capsule in a stable region, salts and the like that form a buffering action are added as necessary.

Microencapsulation method An enzyme such as the aforementioned protease and polyvinyl alcohol are dissolved in a mixture of a polyhydroxy compound such as propylene glycol and water. If the amount of calcium in this solution is 5 ppm or less relative to the enzyme, a calcium salt or the like is added, and other enzyme stabilizers are added as necessary.

Microcapsules using polyvinyl alcohol as a coating material can be prepared from this solution by, for example, the following method.

(i) A method of forming microcapsules by dropping the enzyme solution as minute droplets into water and/or alcohol (ethanol, ethylene glycol, propylene glycol, glycerin, etc.) in which boric acid has been dissolved.

(ii) A method of spraying the enzyme solution with a spray dryer to form microcapsules. In this case, the temperature of the hot air at the inlet is 200° C. or less, preferably 80 to 120° C. to avoid deactivation of the enzyme due to temperature TR.

1ii) A method of dispersing the enzyme solution as minute droplets in a hydrophobic solvent (benzene, hexane, liquid paraffin, etc.) and removing water by heating or reducing pressure to form microcapsules.

Surfactant The detergent composition of the present invention uses general detergent surfactants, including anionic surfactants and cationic surfactants, whose use has been limited in enzyme-containing liquid detergents because they deactivate enzymes. agents can be widely used.

For example, as an anionic surfactant, linear sodium alkylbenzenesulfonate (alkyl: C, o-C1
6) (LAS), α-olefin sodium sulfonate (olefin: C6-C22>(AO8), alkyl6Ai! Sodium (alkyl: C9-C1)
□) (AS), polyoxyethylene alkyl ether fJ [sodium (alkyl: C9-C1□, Eod = 1-20)
(AES), 2-sulfo fatty acid ester sodium (fatty acid: C11
~C1□, alcohol: C-C6) (SFE) Sodium secondary alkanesulfonate (alkane: C12-Cl8
) (SAS), soap (fatty acid-C12-018), sodium alkyl phosphate (alkyl: C9-C1□), etc. Nonionic surfactants include polyoxyethylene alkyl ether (alkyl: C5-C17). .

EOP=4-20) (AE>, polyoxyethylene alkylphenyl ether (alkyl: C6-C12,
[od: 4-20) (APE), etc., and examples of cationic surfactants include cetyltrimethylammonium chloride, stearyldimethylbenzylammonium chloride, and distearyldimethylammonium chloride, and examples of amphoteric surfactants include: , lauryl betaine, and stearyl betaine.

Composition of Detergent Composition The basic composition of the detergent according to the present invention is as follows.

Enzyme-containing i-capsel 0.01-10% (vehicle weight%)
) Surfactant 3-70% water
P# to be blended 5-70%
It is not limited to one type of capsule containing elementary gold. Conventionally, it has been difficult to mix several types of enzymes in a water-based system such as a liquid detergent, but according to the present invention, for example, protea-L1 amyl-L, etc. can be individually microencapsulated. Even if multiple types of microcapsules are mixed together, good storage stability can be maintained.

In the detergent composition of the present invention, in addition to the above basic composition,
Bilgoos commonly used in detergents, such as propylene glycol, ethanol, fluorescent dyes, and citric acid, are optionally added.

[Examples] Hereinafter, the present invention will be explained in more detail with reference to Examples and Comparative Examples.

In addition, in the following examples, protease is the unit described in JP-A-58-134990@, as the enzyme titer unit.
Amylase is specified in Japanese Industrial Standard JIS-7001 (1
972) Note 1 The unit of energy, cellulase, is from the Enzyme Utilization Handbook (Jijinzankan, 1980, supervised by Michio Ozaki) 2
The units described on page 98, the units described on page 422 of the [Handbook for the Use of Soluble Utilization] for lysozyme, and the units described on page 230 of the "Handbook for Enzyme Utilization" were used for lipase.

Example 1 BaC11L described in JP-A-58-134990@
ISSP.

FER)l BP-93 was cultured and the titer was 105 nk.
A protease (API-21) bulk powder of at/m3 was obtained (the titer was measured according to the method described in JP-A-58-134990).

This protease was prepared into a solution containing the following mixture.

Protease (API-21) powder 70g
Calcium acetate 8g Propylene glycol 480g Polyvinyl alcohol (average degree of convergence 2,000, saponification rate 95%) 55g Distilled water 387g After filtering this solution, it was dropped into a saturated boric acid solution through a thin nozzle to form microcapsules. . The microcapsules were separated by filtration and stored in propylene glycol containing 25% water.

The size of the microcapsules was approximately 150-800μ in diameter.

Next, a liquid detergent composition containing propylene glycol in which the above microcapsules were dispersed and having the following composition was prepared.

Composition of composition Weight% polyoxyethylene alkyl ether lit
C14-15, EOP = 15>
15 polyoxyethylene alkyl ether (C12-15, Il:OP = 10>
18 carboxymethylcellulose polyethylene glycol (MW 6,000)
1 Ethanol 10 Sodium citrate dihydrate 8 Microcapsule dispersion in propylene glycol (average protease titer 4,000 nkat/d> 5 Distilled water
42 Comparative Example 1 Protea-t'(API-21> and calcium acetate were dissolved in propylene glycol containing 25% water,
Protease titer 4,000 nkat/7. A composition having the same composition as the liquid detergent of Example 1 was prepared, except that a solution containing 50n14 calcium ions was prepared and used in place of the propylene glycol dispersion of microcapsules.

The liquid detergent compositions of Example 1 and Comparative Example 1 were heated to 38'C (
After storage at 100' F for 10 weeks, the enzyme activity was measured, and the residual activity was 80 to 85% in the case of Example 1 containing microcapsules, but in the case of Example 1 containing protease propylene glycol. In the case of Comparative Example 1, it was below 3% (detection limit).

Example 2 Commercially available lipase isolated from pig pancreas (Amano Mulberry).

Pancreatin TA, lipase value 5,000 U/
9>, Luamirar Ii obtained by culturing Bacillus 5ubtilis (Daiwa Kasei, Talaistase)
120. titer 220.000 U/g> and Bac.
Protease obtained by culturing P. llus 5ubtilis (Daiwa Kasei, Protin AS, titer 600.00
0 PI/'J) using solutions A, B, and
Created C.

Weight% Fox Δ Dan Ω Lipase 5 − −Amylase
5-Protease
- -8 Calcium acetate 0.7
0.9 0.5 Propylene glycol 45
4.5 50 polyvinyl alcohol 6 5
6 (Average degree of polymerization 1, 500. Saponification rate 97%) Distilled water 43.3 44.1 35.5
Next, solutions A, B, and C were each dropped into a boric acid saturated aqueous solution containing 50% ethanol through a thin nozzle to form microcapsules, and treated in the same manner as in Example 1.
Stored in propylene glycol containing 5%.

Next, a liquid detergent composition containing the three types of microcapsules described below was prepared.

Composition of composition Weight % Sodium polyoxyethylene alkyl ether sulfate (C14~
15, EOP = 5> 10 polyoxyethylene alkyl ether (c13-15, EOP
~12> 25 Coconut fatty acid sodium salt 5 Carboxymethylcellulose 1 Lipase microcapsule-propylene dispersion (average lipase titer, 1001
3/rIdl> 3 amylase microcapsule-propylene dispersion (average amylase titer, 3,
300 U/Infl> 3 Protease microcapsule-propylene dispersion (average protease titer, 1,800 nkat/mf!> 10 Diethylene glycol 7 Distilled water
36 Comparative Example 2 Lipase bulk powder, amylase bulk powder, and protease I Kyosei were each dissolved in propylene glycol (containing 25% water) containing 60 mM calcium ions, and the average lipase bulk powder 1
00 [1/d propylene glycol solution, average amylase titer 3,300 tl/d propylene glycol solution, and average protease titer 1,800 nk
Prepare an at/mal propylene glycol solution,
Each was used in place of the microcapsule dispersion of Example 2 to prepare compositions corresponding to detergent compositions A, B, and C.

The liquid detergent compositions of Example 2 and Comparative Example 2 were heated at 38°C (1
In the case of Example 2, in which the enzyme was formulated as microcapsules, the enzyme activity residual rate after storage for 8 weeks at 00"F) was 5-80% for liver-hiff and 80-85% for amylase.
, protease was 80 to 85%, but in the case of Comparative Example 2 in which the enzyme was formulated as a propylene glycol solution, it was 3% (detection limit) or less.

Example 3 Alkaline protease obtained by culturing Bacillus Iicheniformis (manufactured by Sigma,
A solution containing the following mixture was prepared using Product No. o 5380).

Alkaline protease 50g Magnesium chloride 3g Calcium chloride 39 Sodium ascorbic acid monophosphate 4g Propylene glycol
450g polyvinyl alcohol
45 g (average degree of polymerization i, ooo, saponification rate 99%) 445 g of distilled water The above protease solution was dropped into a propylene glycol-water mixture saturated with boric acid to create microcapsules, which were separated by filtration. , stored in propylene glycol containing 25% water.

Next, a gel detergent composition containing propylene glycol in which the microcapsules were dispersed had the following composition.

Composition of composition Weight % polyoxyethylene alkyl ether tii (CM sodium (C
73~,,, EOP = 5> 8 polyoxyethylene alkyl ether (C13~15, EO
P = 10) 25 Sodium stearate 3 Sodium citrate dihydrate 10 Carboxymethylcellulose polyethylene glycol (MW 1,000>
Propylene glycol dispersion of 3 microcapsules (average protease capacity (i[ri, 1,600 nkat
/ mQ) 10 propylene glycol
14 Distilled water 1
6 Comparative Example 3 Alkaline protease (manufactured by Sigma, p 5380)
Prepare a propylene glycol solution (water 25%) containing 1,600 nkat/rtdl and calcium chloride (0.3%), magnesium chloride (0.8%), sodium ascorbate monophosphate (0.4%), A composition having exactly the same composition as the gel detergent of Example 3 was prepared except that this solution was used in place of the microcapsule dispersion of Example 3.

The gel detergent compositions of Example 3 and Comparative Example 3 were heated at 38°C (
The residual rate of enzyme activity in the gel-like detergent composition after storage at 100' F for 10 weeks was 90 to 95% in the case of Example 3 containing microcapsules, but a propylene glycol solution of protease In the case of Comparative Example 3 in which the compound was blended, the amount was 3% (detection limit) or less.

Example 4 Thermostable amylase obtained by culturing Bacillus subtilis (Daiwa Kasei, Talaistase Tt
J20.

titer 220,000 U/9) and Bacill
Thermostable protease (Daiwa Kasei, 4J-E7ase PS, titer 5
00,000 Pυ/9), a solution E having the following composition was prepared.

Weight % Composition DE Thermostable amylase 5 - Thermostable protease 5 Calcium acetate
0.5 0.5 Magnesium acetate
0.5 0.5 diethylene glycol 4545 polyvinyl alcohol 5
5 (Average degree of polymerization 2,000, saponification rate 97%) Natural distilled water
4444 Next, each of the solutions and E is
It was dropped into a saturated boric acid aqueous solution containing 10% ethanol and 20% ethylene glycol through a thin nozzle, and the resulting microcapsules were separated, dispersed and stored in propylene glycol containing 25% water.

Next, the above microcapsules were blended to prepare a dishwashing detergent composition having the following composition.

Composition of composition Weight % Sodium dodecyl sulfate 20 Alcohol ethoxylate 20 (C13-1s,
EO=10 mol) carboxymethyl cellulose
1 Amira 1 Piece Microcapsule Dispersion 6
(Average amylase titer 3,000 U/d) Protease-microcapsule dispersion 6 (Average protease titer t & 1,500 nkat/mi > Distilled water
47 Comparative Example 4 Heat-stable amylase bulk powder and heat-stable protease bulk powder were dissolved in propylene glycol (containing 25% water) containing 50 ml of calcium ions, and the average amylase titer was 3.
,000 U/d and a propylene glycol solution with an average protease titer of 1,500 nkat/d were prepared and used in place of the amylase and protease microcapsule dispersions, respectively, of Example 4, otherwise identical. A dishwashing detergent composition was manufactured by JAl.

The detergent compositions of Example 4 and Comparative Example 5 were heated at 38°C (100°C
The residual activity of the enzyme in the detergent composition after storage for 10 weeks at
In the case of 90-95% with amylapylic acid and protease.
However, in the case of Comparative Example 4 in which the enzyme was formulated as a propylene glycol solution, it was 3% or less (detection limit).

Example 5 Separated from chicken eggs, zozyme (manufactured by Sigma).

A solution containing the following mixture was prepared using Product No. Q L7001).

Lysozyme 20g Calcium chloride 3g Propylene glycol 520g Sodium ascorbic acid monophosphate 2g Polyvinyl alcohol 45g (Average degree of polymerization 700. Saponification rate 87%) Ethanol
20g distilled water
Microcapsules were prepared from 390 g of this solution using a spray dryer equipped with a rotating disk type disperser. The size of the microcapsule is approximately 20~20mm in neck diameter.
It was 100μ. Next, a shampoo composition having the following composition containing propylene glycol in which the microcapsules were dispersed was prepared.

Composition Weight % Sodium polyoxyethylene lauryl ael sulfate
10 Sodium stearate 5 Sodium laurate
8 propylene glycol
47 Ethyl alcohol 15
Lysozyme microcapsule propylene glycol dispersion (average lysozyme titer 400 U/d> 5 distilled water 10 soils) Lysozyme, calcium chloride (0.3%), sodium ascorbic acid monophosphate (0.2%) A shampoo composition having exactly the same composition as in Example 5 was prepared, except that a propylene glycol solution of lysozyme with a titer of 400 tl/d was prepared, and this solution was used in place of the lysozyme microcapsule dispersion of Example 5. Created.

The shampoo compositions of Example 5 and Comparative Example 5 were heated to 30″C.
The residual activity of the enzyme in the shampoo composition after being stored for 3 months in Example 5, which was formulated as microcapsules, was
In the case of Comparative Example 5, in which the enzyme was formulated as a propylene glycol solution, it was 3% (
detection limit).

[Effects of the Invention] The present invention provides a detergent composition in which a stabilized enzyme solution is microencapsulated and blended into a liquid or gel detergent.According to the present invention, (1) in the detergent, Since the enzyme is coated with polyvinyl alcohol, which is insoluble but soluble in water, the enzyme is maintained in a stable state during storage of the detergent; (2) the enzyme tends to be deactivated in the presence of water; Since enzymes do not come into direct contact with surfactants and other detergent builders during detergent storage, the storage stability of enzymes is significantly improved compared to conventional enzyme-containing liquid detergents; (3) Deactivation of enzymes; Therefore, it can be applied to cationic surfactants or anionic surfactants for which the detergency of enzymes could not be considered, and (4) mixing of multiple types of enzymes, which was difficult in the presence of water such as in liquid detergents, is possible. However, excellent effects such as those made possible by using separately microencapsulated Bata elements can be obtained.

Claims (1)

[Scope of Claims] 1) A detergent composition comprising enzymes coated with polyvinyl alcohol as microcapsules and uniformly dispersed in a liquid or gel detergent. 2) The detergent composition according to claim 1, wherein the enzyme is microencapsulated in a state dissolved or dispersed in a water-containing polyhydroxy compound. 3) The average degree of polymerization of polyvinyl alcohol is 200 to 4,
000 and a saponification rate of 80% or more. 4) The liquid or gel detergent is mainly composed of nonionic surfactants and/or anionic surfactants, and contains 70%
Claim 1, which contains moisture of % or more
The detergent composition described in Section.