JPS6299331A - Cancer preventing food - Google Patents

Abstract

PURPOSE:A cancer preventing food comprising leaves of carrot as an essential component, which is blended with a compounding component. CONSTITUTION:Leaf parts of Daucus carota L. (umbelliferous, annual or biennial herb), an edible plant, dried, ground or thinly cut, immersed in an aqueous solvent, allowed to stand and extracted with the aqueous solvent such as an aqueous solution of ethanol, etc. for 24-48hr or extracted under reflux by heating (60-90 deg.C). Then, the mixture is filtered, the filtrate is concentrated or reduced under pressure to give a dried substance. It is purified and the prepared white powder is contained as an active ingredient, to give a cancer preventing food. A preferable food state of the leaves of Daucus carota L. is tea leaves. The leaf parts of Daucus carota L. may be further blended with leaf parts of Raphanus sativus L., citrus fruits, SAKE lees, leaf parts of Petroselium crispum Nym., etc., dried powder and extract thereof, and one or more dyestuff component of Glycine max and Virgna angularis Ohwi et Ohashi as a compounding component.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a cancer preventive food, and more specifically to a common edible plant, carrot (Daucus carota L.
, ) leaves are an essential ingredient, and this essential ingredient is radish (Ra) leaves.
Using ordinary edible foods such as the leaves of Phanus 5ativus L., it is extremely safe and prevents the increase in oxides such as lipid peroxide in the body, preventing obesity and various harmful effects caused by an increase in lipid peroxide. The aim is to provide a cancer-preventing food that can eliminate cancer and also exhibit cancer-preventing effects.

(Prior art and its disadvantages) Cancer is a general term for malignant tumors and sarcomas, and is one of the oldest diseases that is thought to have existed with the advent of humanity.
It can be said that it is a new disease in that the cause and treatment method have not yet been established, and it is an incurable and fatal disease.

Currently, chemotherapy for cancer is performed as an adjunct to surgical procedures, but it has only been statistically shown to have a survival-prolonging effect on specific cancers.

These are alkylating agents, antimetabolites, antibiotics, hormonal agents, poly-Pis, and the like.

The inventor had been conducting extensive research on edible foods, and recently discovered the results of epidemiological research by Chikayoshi Nagata (National Cancer Center Research Institute), ``High-fat diets, in other words, excessive fat intake.'' There are reports that there is a correlation between colon cancer, prostate cancer, skin cancer, and lung cancer, and a report that ``peroxidation of ingested fat promotes carcinogenesis'' (Science magazine, 1983゜6). (Japanese issue), we attempted to develop an edible food that could prevent cancer.

In other words, there are effective anticancer agents for the treatment of alkylating agents, antimetabolites, antibiotics, hormonal agents, multi-VM drugs, etc., which have conventionally been merely auxiliary methods and whose effects have hardly been recognized. In today's world, it is more urgent to prevent cancer, and we have begun research to find out whether it is possible to prevent cancer through food.

As disclosed in the research paper by Chikayoshi Nagata, α
- Antioxidants such as tocopherol, BHA, and BIT are said to have the effect of preventing cancer.

However, these antioxidants are chemically synthesized products.
-Tocopherol and Bl (A, BIT, etc.)
Its repeated use has long been considered to be highly problematic in terms of safety.

On the other hand, due to improvements in the food situation in recent years, many people have adopted a high-carbohydrate, high-calorie diet from infancy, which increases lipid peroxide in the body, causing adult diseases and triggering the onset of cancer. It is thought that this is likely to occur. (Refer to the above report) Therefore, all you need to do is improve your eating habits, but eating habits are habits that have been in place since childhood, so it is extremely difficult to change those habits. As a result, the situation is getting worse, including eating too much lipid peroxide and overworking and unbalancing the body's metabolic function.

Therefore, even if you do not improve your diet, you can consume these foods in combination with high-carbohydrate and high-calorie foods to exert antioxidant ability in the body and eliminate the tendency to have high lipid peroxidation. We completed this invention based on the belief that food products with extremely safe antioxidant properties can prevent the onset of cancer, rather than using antioxidants such as BIT, which have serious safety problems.

(Solution) As mentioned above, this inventor has been engaged in research on edible plants for many years, and in the course of his research, he discovered that certain foods have extremely high antioxidant ability, which he has already clarified. This invention was arrived at through the progress of the research.
This relates to a cancer-preventing food containing the leaves of L. as an essential ingredient and other ingredients mixed therein.

(Structure of the invention) In this invention, carrot (Daucus carot)
aL,) is an annual or biennial plant of the Umbelliferae family that is often eaten, and its roots are usually eaten.

In this invention, the carrot (Daucus c.
The leaf part (aerial part) of Arota L.) is used.

Carrots like this (Daucus earota, L
, ) can be used as an essential component of the cancer prevention food according to the present invention by pulverizing the leaves, or by obtaining an extract or extract powder by the extraction method described below.

In addition, when powdering, avoid high heat treatment as much as possible.
It is desirable to form it into a dry powder using a method such as freeze-drying.

The extraction method is not limited to the following example.

First, the leaves are dried and crushed or chopped.

This pulverized product can also be used as an active ingredient in cell activation foods according to the present invention.

Alternatively, this pulverized material is first treated with an aqueous solvent such as ethanol water, physiological saline, organic acid water, or water, or an aqueous solution of a salt of each organic acid with a concentration of 5χ (weight) or less,
Extract.

Extraction is carried out by standing for 24 to 48 hours, or by heating under reflux.

The aqueous solvent used as the extract is usually 10 to 20%
Use double the amount.

When performing heating extraction, the heating temperature is 60° to 90°
Heat to reflux for about 30 to 80 minutes.

Thereafter, it is separated by filtration, and the filtrate is -a-condensed under reduced pressure or normal pressure so that the extract content becomes about 30χ.

This concentrate is dried under reduced pressure to obtain a dry product, or the extract is freeze-dried or even spray-dried to obtain a dry product.

The dry powder obtained is a green powder.

Although this dried product has low purity, it can be used as is as a cancer prevention food.

Next, this dried product was dissolved in water, filtered, and the filtrate was dissolved in n-
Separation and purification with hexane-water, further water in the water eluate layer was distilled off under reduced pressure to obtain a dry powder, this dried product was dissolved in water, further separation and purification with n~butanol-water, and further after drying under reduced pressure. When this dry powder is dissolved in methanol and separated by column chromatography, a white powder is obtained.

This white powder is considered to have the most effective antioxidant effect and is an essential component of cancer prevention foods, and it is estimated that this component is present in the largest amount in the leaves.

However, unfortunately, the structure of this powder has not yet been analyzed, and this will be a future research topic for this inventor.

With this invention, carrot (Daucus carota L.
Other preferred food forms of the leaves of , ) include tea leaves.

To make tea from carrot (Daucus carota L.) leaves, follow the conventional method.
aucuscarota L.) leaves are collected and cut into thin pieces, steam-heated at about 98°C for 10 seconds, then coarsely rubbed for several tens of minutes while heating at about 70-75°C, and then heated at 35°C. Knead at ~40℃ for about 20 minutes, then 5 more
Heat at 0 to 55℃ for about 30 to 40 minutes, and finally at 60℃.
It may be dried at ~70°C for 20 to 30 minutes.

The carrot tea leaves can be prepared by pouring hot water over them like regular tea and making the extracted water drinkable, or by crushing the tea leaves and mixing the powder with other additives or without adding other additives. It may be mixed into food and used for human consumption.

In this invention, carrots (Dauc) as described above are used.
In addition to the leaves of radish (us carota L,)
Dried powder of leaves of Raphanus 5ativus L, and/or extract thereof, black bean (Glycine
max) pigment components, dried citrus fruit powder and/or its extract, dried sake lees powder, parsley (Petrosel
dried powder and/or extract thereof of the leaves of Nym.
One or more components selected from the pigment components of ularis ohwi et ohashi) may be added as a compounding component.

In this invention, Japanese radish (Raphanus 5at)
ivus L.) is often eaten, and its roots are usually eaten.

In this invention, the radish (Raphanus)
The leaves of Sativus L.) are used.

This kind of radish (Raphanus 5ativus)
In order to use the leaves of the carrot (Daucus carota) as a compounding ingredient of the cancer prevention food according to the present invention,
It may be processed in the same manner as the leaves of L.) and powdered, or the extract or extract powder may be obtained by the extraction method described above.

With this invention, radish (Raphanus 5ativu)
Other preferred food forms of the leaves of s L,) include:
There are tea leaves.

Radish (Raphanus 5ativus L,)
In order to use the leaves of Daucus as tea leaves,
It can be treated in the same way as the leaves of Carota L.).

Parsley (Petrosel) used in this invention
iumcrispuIIINym, ) may be made into a dry powder and/or an extract thereof in the same manner as the above-mentioned radish (Raphanussativus L.) leaves.

Furthermore, parsley (Petroseri) used in this invention
umcrispum Nym,) is a member of the Apiaceae family (Pe
Troselium) is a biennial herb whose roots are usually eaten.

Glycine ma used in this invention
x) Pigment component, Azuki (Virgna angul)
The pigment component of Aris Ohwi et Ohashi) refers to the red or black pigment obtained from the pulverized product thereof.

In addition, black soybean (Glycine) used in this invention
max) is an annual plant of the legume family, soybean (Glycin).
e max) seeds with black seed coats.

In order to use such adzuki beans or black beans as a compounding ingredient in the cancer prevention food used in the present invention, first, the seeds of azuki beans or black beans are coarsely ground and the seed coats are collected, and in some cases, the seed coats are not separated. The coarsely ground product of only the seed coat or the whole seed is further ground to a final pulverized particle size of about 50 mesh to 20 mesh, preferably 40 mesh to 30 mesh.

Next, 5 to 20 times as much water was added to this pulverized material, and the mixture was heated at room temperature for 12
Leave for 48 hours, preferably 20 hours to 36 hours.

Next, the whole is heated at about 60° C. for 1 to 2 hours, filtered, and the filtrate is concentrated under reduced pressure and heat.

After concentrating to about 1/2 to 175%, it is freeze-dried or spray-dried to obtain a reddish-brown or black powder.

This yield varies depending on the degree of concentration of the filtrate, but is 174 to 1/10 of the weight of the seeds.

As the azuki or black bean pigment component used in this invention, both the pigment-containing concentrate and the powder pigment component can be used.

Citrus fruits used in this invention are generally referred to as mandarin oranges, and belong to the Rutaceae subfamily Rutaceae (Rutaceae).
urus), the genus Forutunella, and the genus Poncirus.Specifically, mandarin orange, tangerine, lemon, yuzu, grapefruit, buntang, orange, citron, lime, kunembo, and karatachi. , kumquat, tachibana, kantah, etc. can be specifically exemplified.

The peel of such citrus fruits may be included in cancer prevention foods as an extract, or may be prepared as a dry powder and included in foods.

A dry powder, preferably a freeze-dried pulverized product, or an extract that is freeze-dried and pulverized by a spray drying method during the extraction process, or an extract may be prepared in the same manner as for the carrot leaves.

The dried sake lees powder used in this invention is preferably a dried product of sake mash produced as a by-product during the brewing of sake, from which alcohol odor has been completely emitted.

Alternatively, it may be an extract of this dried product, and in that case, it may be used in the same manner as the carrot leaf part described above.

The dry powder of brown algae used as a compounding ingredient in this invention is one or more kinds of dry powder selected from wakame, hijiki, sargassum, Japanese kelp, myrtle, Iwahige, sea fan, and sandweed, and is usually a dry product of marine products. Any of the above-mentioned brown algae having a water content of about 10 to 15X and further adjusted to have a water content of about 3χ after pulverization can be suitably used.

With this invention, carrot (Daucus carota L.
For essential ingredients such as dried products, dried powders, or extracts, including leaves of ), whether they are used as a single ingredient or in a mixture with one or more other ingredients, the amount consumed per serving is calculated as the raw material powder. For adults, it is desirable to set the dose in the range of 500 mg to 10,000 mg.

In this invention, carrot (Daucus caro
Other formulation ingredients are mixed to supplementally increase the effect of the leaves of ta L,).

In order to prepare the cancer preventive food according to the present invention by mixing such ingredients, suitable final forms of the food include powders, granules, pills, staple food, confectionery (kutsky), tea preparations, and soft drinks. All forms commonly used as food can be adopted.

(Effects) As detailed above, this invention
carota L.) leaves as an essential ingredient, which is mixed with other ingredients to prevent cancer.
Increases fat gain and high peroxidized lipid tendency due to high calorie diet.
1! It can be cured without forcing people to eat high-calorie foods or avoiding them, and therefore has the effect of preventing cancer that occurs as a result of these symptoms.

Next, Examples and Test Examples will be described to further clarify the effects of the present invention.

Test Example 1 The following test was conducted using components (1) to (8) in the following table.

Linoleic acid (manufactured by Wako Pure Chemical Industries, Ltd.) Log Tween 80 surfactant (manufactured by Atlas, USA) 0.8g and the ingredients in the table below (1
) or (8) or Comparative Example 1 (BH Hachiheko Pure Chemical Industries, Ltd.), Comparative Example 2 (B) IT Wako Pure Chemical Industries, Ltd.), Comparative Example 3 (α-tocopherol manufactured by Tokyo Kasei Co., Ltd.) O,
Prepare 11 samples to which each Ig was added, and add this sample to 60'
It was placed in a constant temperature bath at room temperature C and aerated with an air pump for 3 hours.
The oxidation inhibition rate after 3 hours is pov <peroxide value) and T
Measured by BAV (thiobarbic acid value).

The results are shown in Table 1.

Also, linoleic acid (manufactured by Wako Pure Chemical Industries) Log Tween 8
0.0 (surfactant manufactured by Atlas, USA) was added, and the sample was placed in a constant temperature bath at 60°C, and heated with an air pump for 30 minutes.
Components (1) to (8), Comparative Example 1 (B) IA Wako Pure Chemical
Table 2 shows the results of the investigation on Comparative Example 2 (Bl (manufactured by T Wako Pure Chemical Industries, Ltd.), Comparative Example 3 (α-tocopherol, manufactured by Tokyo Kasei Co., Ltd.).

Table 1 Table 2 Test Example 2 Commercially available radish leaves ^, parsley leaves B, adzuki bean pigment component C, @ bean pigment component D, dried sake lees E, and dried seaweed F were each made into 1N products.

Separately commercially available carrot leaves were prepared.

The wakame seaweed used was a commercially available dried wakame seaweed that was pulverized to about 30 mesh passes (punch hammer pulverization method) and then adjusted to a moisture content of 3χ.

2 kg of dried carrot leaves, radish leaves, and parsley leaves each? Made by A, the dried product is crushed,
This pulverized product was first refluxed for 60 minutes using 10 liters of water, and then extracted while heating at 80°C.

This extraction was repeated three times.

Thereafter, the mixture was filtered, the filtrate was concentrated to 1/10, and the extract was spray-dried. 760 g of powder was obtained.

Using these powders, powders were prepared according to the following formulation and used for testing.

Carrot leaf extract powder 300mg Extract or pigment component, etc. 250mg Any one of A to F dried yuzu powder 400mg (epidermis after squeezing the juice from yuzu) Milk w50IIIg Six compositions A to F above were obtained. Ta.

Mouse transplantable methylcholanthrene sarcoma (Me th
The survival effects of the above compositions A to F on mice transplanted with -A) were investigated in animal experiments.

Mice i (BALB/C strain) 1 group of 50 mice, I
Meth-A cells for X 10 h were intraperitoneally transplanted into mice, and the mice were allowed to eat a feed containing the above composition at 1000 mg/kg body weight.

Separately, a comparison group was given only a normal mixed feed for control. The results are shown in Table 3.

Section 3-1 As is clear from the above results, the cancer preventive food according to the present invention has excellent effects.

Claims (5)

[Claims] (1) Carrot (Daucus carota L.)
A cancer-preventing food made of leaves as an essential ingredient and mixed with other ingredients. (2) The carrot (Daucus carota L.
．． The cancer preventive food according to claim (1), characterized in that the leaves of the following are dried leaves and/or an extract thereof. (3) The compounded ingredients are fillers, excipients, flavorings, fragrances,
The cancer preventive food according to claim (1) or (2), characterized in that it is one or more ingredients selected from vitamins, minerals, etc. (4) The compounded ingredient is radish (Raphanus s
ativus L. ) and/or its extract, pigment components of black soybean (Glycine max),
Dry powder of citrus fruit and/or its extract, dried powder of sake lees, parsley (Petroselium crispu)
m Nym. ) and/or its extract, azuki bean (Virgna angularis Ohw)
The cancer preventive food according to any one of claims (1) to (3), characterized in that the cancer preventive food is one or more components selected from among the pigment components of . (5) The scope of claims characterized in that the compounded ingredient contains at least one kind of dry powder of brown algae selected from wakame, hijiki, sargassum, Japanese kelp, myrtle, rockfish, sea fan, and sandweed. The cancer preventive food according to any one of paragraphs (1) to (4).