JPS6279791A - Production of 6'-galactosyl lactose utilizing lactose-assimilating microorganism - Google Patents
Production of 6'-galactosyl lactose utilizing lactose-assimilating microorganismInfo
- Publication number
- JPS6279791A JPS6279791A JP60219707A JP21970785A JPS6279791A JP S6279791 A JPS6279791 A JP S6279791A JP 60219707 A JP60219707 A JP 60219707A JP 21970785 A JP21970785 A JP 21970785A JP S6279791 A JPS6279791 A JP S6279791A
- Authority
- JP
- Japan
- Prior art keywords
- lactose
- microorganism
- genus
- galactosyl
- penicillium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 title claims abstract description 11
- 244000005700 microbiome Species 0.000 title claims description 24
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 17
- 239000008101 lactose Substances 0.000 claims abstract description 16
- 241000228143 Penicillium Species 0.000 claims abstract description 9
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 8
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 8
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 6
- 241000223259 Trichoderma Species 0.000 claims abstract description 5
- 241000228212 Aspergillus Species 0.000 claims abstract description 4
- 240000000064 Penicillium roqueforti Species 0.000 claims abstract 2
- 235000002233 Penicillium roqueforti Nutrition 0.000 claims abstract 2
- ATYSZLKTHMZHJA-UXLSSDPBSA-N (3R,4R,5S,6R)-6-(hydroxymethyl)-2-[(3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]-5-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxane-2,3,4-triol Chemical compound C1([C@H](O)[C@@H](O)[C@@H](O)[C@H](O1)CO)C1(O)[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@H](O2)CO)[C@H](O1)CO ATYSZLKTHMZHJA-UXLSSDPBSA-N 0.000 claims description 4
- 101100400378 Mus musculus Marveld2 gene Proteins 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 239000000047 product Substances 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 3
- 239000006227 byproduct Substances 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 241000186000 Bifidobacterium Species 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 102000005936 beta-Galactosidase Human genes 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 235000020256 human milk Nutrition 0.000 description 2
- 210000004251 human milk Anatomy 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 150000004044 tetrasaccharides Chemical class 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 238000005918 transglycosylation reaction Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
皇粟上■■朋分…
本発明は、ビフィズス菌の増殖促進因子並びに便性改善
因子として利用される６′−ガラクトシルラクトースの
製造方法に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing 6'-galactosyllactose, which is used as a growth promoting factor for bifidobacteria and as a fecal quality improving factor.
ガラクトシルラクトースは、乳糖にガラクトースが結合
したとｉｉ遁総称であり、そのうちの６′−ガラクトシ
ルラクトースは人乳中に含まれていて、育児用粉乳に添
加してビフィズス菌の増殖促進因子及び便性改善因子と
して利用されている。Galactosyllactose is a general term for galactose bound to lactose, and 6'-galactosyllactose is contained in human milk and is added to powdered milk for infants to promote the growth of bifidobacteria and improve faecal quality. It is used as an improvement factor.
従来色茨血
従来、ガラクトシルラクトース（以下ＧＬと略記する）
の製造法として、乳糖にアスペルギルス・オリーゼが生
産するβ−ガラクトシダーゼを作用させることにより合
成する方法（特公昭５８−２０２６６）が知られている
。Conventionally colored thorn blood Conventionally, galactosyl lactose (hereinafter abbreviated as GL)
As a manufacturing method, a method is known in which lactose is synthesized by reacting β-galactosidase produced by Aspergillus oryzae (Japanese Patent Publication No. 58-20266).
しかし、この方法ではβ−ガラクトシダーゼによる糖転
移反応によってＧＬを合成するため、少くとも４種類の
ＧＬと４糖類及び５１ｆＪ類が同時に合成される。した
がって、人乳中に含まれる６′−ＧＬのみを合成するこ
とができないので、上述した４種類以上のＧＬと４糖類
及び５糖類とから成る混合物を育児用粉乳に添加するこ
とによって、該混合物中に含まれる６’−ＧＬを利用し
ているのが現状であり、６’−ＧＬの有効な利用上の観
点から、６’−ＧＬのみを合成し得る方法の開発が要望
されている。However, in this method, since GL is synthesized by transglycosylation reaction using β-galactosidase, at least four types of GL, tetrasaccharides, and 51fJs are simultaneously synthesized. Therefore, since it is not possible to synthesize only 6'-GL contained in human milk, by adding a mixture consisting of the above-mentioned four or more types of GL and tetrasaccharides and pentasaccharides to infant milk powder, the mixture can be synthesized. Currently, 6'-GL contained in the 6'-GL is used, and from the viewpoint of effective utilization of 6'-GL, there is a demand for the development of a method capable of synthesizing only 6'-GL.
。日が１ンしようとする。 壱
本発明者は、上述した現状に鑑み、６’−ＧＬのみを効
率よく製造し得る方法について検討した結果、アスペル
ギルス属、ペニシリウム属、ならびにトリコデルマ属に
属するラクトース資化性菌が、乳糖を基質として６’−
ＯＬのみを産出することを見出し、本発明をなすに至っ
た。. The sun is about to set. 1. In view of the above-mentioned current situation, the present inventor investigated a method for efficiently producing only 6'-GL, and found that lactose-assimilating bacteria belonging to the genus Aspergillus, Penicillium, and Trichoderma use lactose as a substrate. as 6'-
It was discovered that only OL was produced, and the present invention was completed.
すなわち、本発明は、微生物を利用して６′−〇しのみ
を効率よく製造し得る方法を提供することを目的とする
。That is, an object of the present invention is to provide a method for efficiently producing 6'-〇 using microorganisms.
以下に本発明の詳細な説明する。The present invention will be explained in detail below.
光皿■盪底
本発明の特徴は、アスペルギルス属（＾ｓｐｅｒｇｉｌ
−Ｉｕｓ）、ペニシリウム属（Ｐｅｎｉｃｉ Ｉｌｉｕ
ｍ）及びトリコデルマ属（Ｔｒｉｃｈｏｄｅｒｍａ）に
属する微生物から成る群から選択される、乳糖から６′
−ガラクトシルラクトースの産生能を有する微生物を、
乳糖を含有する培地中に培養し、得られた培養物から６
′−ガラクトシルラクトースを分離、採取することにあ
る。The characteristics of the light dish bottom of the present invention are as follows:
-Ius), Penicillium (Penici Iliu)
m) and microorganisms belonging to the genus Trichoderma.
- A microorganism capable of producing galactosyllactose,
6 from the culture obtained by culturing in a medium containing lactose.
The purpose is to separate and collect '-galactosyllactose.
５題点を解ンするための手
本発明において利用する微生物は、アスペルギルス属ま
たはペニシリウム属もしくはトリコデルマ属に属するラ
クトース資化性菌であって、アスペルギルス・オリゼ（
Ａｓｐｅｒｇｉｌｌｕｓ ｏｒｙｚａｅ） ＩＦＯ４１
３５、ペニシリウム・ロツクフオルテ（Ｐｅｎｉｃｉｌ
ｌｉ−ｕｍ ｒｏｑｕｅｆｏｒｔｉ） ＩＦＯ４６２２
ならびにトリコデルマ・ハルジヤヌム（Ｔｒｉｃｈｏｄ
ｅｒｍａ ｈａｒｚｉａｎｕｍ） ５ＢＴ７０４５ （
微工研菌寄第８３３８号）を例示し得る。The microorganism used in the present invention is a lactose-assimilating bacterium belonging to the genus Aspergillus, the genus Penicillium, or the genus Trichoderma, and Aspergillus oryzae (
Aspergillus oryzae) IFO41
35. Penicillium rockfuorte (Penicillium
IFO4622
as well as Trichoderma harzianum (Trichod
erma harzianum) 5BT7045 (
An example of this is ``Feikoken Bibori No. 8338''.
これらの微生物は、いずれも公知の菌種であって、上掲
の各寄託番号で保管されていて入手可能である。All of these microorganisms are known species, and are stored and available under the above-mentioned deposit numbers.
本発明では、まず、上掲の各微生物を下記に示す組成の
基本培地を用いて前培養を行なって微生物を増殖させる
。In the present invention, first, each of the microorganisms listed above is precultured using a basic medium having the composition shown below to grow the microorganisms.
前培養のための基本培地組成：
乳１）ｆ１ ３０ ｇ
Ｋ、 ＩＩ Ｐ □帰 １ｇ
Ｍｇ５ｋ７Ｈ２０ｏ、ｓ ｇ
ＮａＮＯｉ ２ ｇ
ＫＣＩ Ｏ，５ｇ
ＦｅＳＯＩ＋ ０．０１ｇ
水 １）
ｐＨ６，０
次いで、微生物を上記前培養のための培地中で増殖させ
たものを下記に示す組成の基本培地を用いて本培養を行
なって６’−ＧＬを生産する。Basic medium composition for preculture: Milk 1) f1 30 g K, II P □ return 1g Mg5k7H20o, s g NaNOi 2 g KCI O, 5g FeSOI + 0.01g water 1) pH 6,0 Then, microorganisms were subjected to the above preculture 6'-GL is produced by main culture using a basic medium having the composition shown below.
本培養のための基本培地組成：
乳ｔＪ！ １５０ ｇ
Ｋ、ＩＦＯ嶋 １ｇＭｇ５Ｏ＋４４
８２０ ０．５ ｇ
ＮａＮＯヨ ２ｇ
ＭＣｌ ０．５ ｇ
ＦｅＳ０４ ０．０１ｇ
水 １７！
ｐｏ ４〜９
なお、上記各培地の使用に当っては、微生物が生育上利
用し得る他の炭素源、窒素源及び無機塩、さらにはその
他の栄養分を適宜添加して含有させることができる。Basic medium composition for main culture: Milk tJ! 150 g K, IFO Shima 1gMg5O+44
820 0.5 g NaNO 2g MCl 0.5 g FeS04 0.01g Water 17! po 4 to 9 In addition, when using each of the above-mentioned media, other carbon sources, nitrogen sources, inorganic salts, and other nutrients that can be used by microorganisms for growth can be appropriately added and contained.
因に、本発明で利用する上記微生物を保存するには下記
に示す培地を用いるとよい。Incidentally, in order to preserve the above-mentioned microorganisms used in the present invention, it is preferable to use the culture medium shown below.
保存培養培地組成：
バレイショ浸出液 ２００ｇ
グルコース ２０ ｇ
寒天 １５ ｇ
水 １）
本発明において、上記微生物を利用して６′−ＧＬを産
生ずるには、該微生物を前述のようにして増殖させたも
のを、上記本培養のための培地に接種してｐＨ４〜９．
２０〜４０℃、好ましくは３０℃前後で通常５〜１５日
間振とう培養を行なう。Preservation culture medium composition: Potato infusion 200 g Glucose 20 g Agar 15 g Water 1) In the present invention, in order to produce 6'-GL using the above microorganism, the microorganism grown as described above is used. , inoculate the medium for the above main culture and adjust the pH to 4 to 9.
Shaking culture is usually carried out at 20 to 40°C, preferably around 30°C, for 5 to 15 days.
参考として、アスペルギルス・オリゼＩＦＯ４１３５、
ペニシリウム・ロツクフオルテＩＦＯ４６２２及びトリ
コデルマ・ハルジヤヌムＳＢＴ ７０４５ （微工研凹
寄第８３３８号）の各微生物の１０日間の培養における
ｐＨと温度条件が６’−ＯＬの産生量に及ぼす影響を開
べた結果を添付の第１図（ａ及びｂ）に示す。For reference, Aspergillus oryzae IFO4135,
Attached are the results of determining the effects of pH and temperature conditions on the production amount of 6'-OL during a 10-day culture of each microorganism, Penicillium rockfuorte IFO4622 and Trichoderma harzianum SBT 7045 (Feikoken Kogyose No. 8338). 1 (a and b).
第１図にみられるとおり、各微生物とも培養時のｐＨ４
〜９及び温度２０〜４０℃において６’−ＧＬの産生量
が高くなることが認められる。As shown in Figure 1, each microorganism has a pH of 4 when cultured.
It is observed that the amount of 6'-GL produced increases at temperatures between 9 and 20 to 40°C.
本発明に従って、上記微生物を上述のようにして培養し
て得られる培養液中には糖質として未反応の乳糖と６’
−ＧＬのみが検出され、他の糖質の産生はみられない。According to the present invention, the culture solution obtained by culturing the microorganism as described above contains unreacted lactose and 6'
- Only GL is detected, and no production of other carbohydrates is observed.
上記培養液中の糖質を薄層クロマトグラフィーにより測
定した結果を薄層クロマトパターンで示すと添付の第２
図のとおりである。The results of measuring carbohydrates in the above culture solution by thin layer chromatography are shown in the attached 2nd chromatography pattern.
As shown in the figure.
培養終了後の培養液から６’−ＧＬを分離、採取するに
は公知の手法を通用して行ない得る。例えば、上記培養
液もしくはそれを濾別して得られた濾液を減圧下に濃縮
して培養液中に残存する乳糖を析出させて分離、除去し
た後、更にエシェリヒア・コリ （Ｅｓｃｈｅｒｉｃｈ
ｉａ ｃｏｌｉ）由来のβ−ガラクトシダーゼを作用さ
せて培養液中に残存する乳糖を分解し、ついで該培養液
を活性炭のカラムに通して６’−ＧＬのみを吸着させ、
この吸着した６′−ＧＬを２０％エタノールで溶出させ
ることにより、精製した６’−ＧＬが得られる。6'-GL can be separated and collected from the culture solution after completion of culture using known techniques. For example, the above culture solution or the filtrate obtained by filtering it is concentrated under reduced pressure to precipitate, separate and remove lactose remaining in the culture solution, and then Escherichia coli (Escherichia coli)
ia coli) to decompose the lactose remaining in the culture solution, and then pass the culture solution through an activated carbon column to adsorb only 6'-GL,
By eluting this adsorbed 6'-GL with 20% ethanol, purified 6'-GL is obtained.
このようにして得られた６’−ＧＬの構造をメチル化分
析により調べた結果、２．３，４．６−テトラ−０−メ
チル−１，５−ジー０−アセチル−ガラクチトール１．
２．３．４− ）ソー０−メチル−１，５，６−トリー
０−アセチル−ガラクチトール、２，３．６−ｏ−メチ
ル−１，４，５−トリーアセチル−ソルビトールが検出
されたことから、純粋な６′−ガラクトシルラクトース
であることが確認された。The structure of 6'-GL thus obtained was investigated by methylation analysis, and the results showed that 2.3,4.6-tetra-0-methyl-1,5-di-0-acetyl-galactitol 1.
2.3.4-) So-0-methyl-1,5,6-tri-0-acetyl-galactitol and 2,3.6-o-methyl-1,4,5-triacetyl-sorbitol were detected. Therefore, it was confirmed that it was pure 6'-galactosyllactose.
叙上のように、本発明によると、微生物を利用して乳糖
から直接的に、且つ他の糖質生成物を伴なうことなく、
６’−ＧＬのみを効率よく製造し得るので、ビフィズス
菌の増殖促進因子及び便性改善因子として有用な６’−
ＧＬの製造上有益であると言える。As mentioned above, according to the present invention, lactose can be produced directly from lactose using microorganisms and without accompanying other carbohydrate products.
Since only 6'-GL can be efficiently produced, 6'-GL is useful as a growth promoting factor for bifidobacteria and as a fecal quality improving factor.
It can be said that this is useful in the production of GL.
以下に実施例を示して本発明を具体的に説明する。EXAMPLES The present invention will be specifically described below with reference to Examples.
実施例１
アスペルギルス・オリゼ（Ａｓｐｅｒｇｉｌｌｕｓ ｏ
ｒｙｚａｅ）ＩＦＯ４１３５の菌株を前述した組成の前
項＃培地１００ｍ１に接種し、３０°Ｃで５日間培養し
た。Example 1 Aspergillus oryzae
ryzae) IFO4135 was inoculated into 100 ml of # medium having the composition described above, and cultured at 30°C for 5 days.
上述のように得られた前培養液１００ｍ １２を、前述
した組成の本培養培地５１に接種し、３０℃で１０日間
振とう培養した。得られた培養液を遠心分離して菌体を
除去した後、減圧濃縮して培養濾液中の乳糖を析出させ
て除去した。ついで、得られた培養濾液を１５ｃｍＸ３
０■の活性炭カラムに通じて６′−ＧＬを吸着させた後
、５％エタノール３１を通じて残存している乳糖を溶出
除去した。ついで、２０％エタノール３１を通じて得た
溶出区分を減圧濃縮した後、凍結乾燥して白色の６’−
ＧＬの粉末１５０ｇを得た。100 m 12 of the preculture solution obtained as described above was inoculated into main culture medium 51 having the composition described above, and cultured with shaking at 30° C. for 10 days. The obtained culture solution was centrifuged to remove bacterial cells, and then concentrated under reduced pressure to precipitate and remove lactose in the culture filtrate. Then, the obtained culture filtrate was placed in a 15 cm x 3
After adsorbing 6'-GL through a 0.0 μl activated carbon column, remaining lactose was removed by elution through 5% ethanol. Then, the elution fraction obtained through 20% ethanol 31 was concentrated under reduced pressure, and then freeze-dried to obtain a white 6'-
150 g of GL powder was obtained.
実施例２
ペニシリウム・ロツクフオルテＩＦ０４６２２の菌株を
用いて実施例１に記載したと同様の方法により、６’−
ＧＬの粉末５０ｇを得た。Example 2 6'-
50 g of GL powder was obtained.
実施例３
トリコデルマ・ハルジヤヌムＳＢＴ ７０４５（ｍ１研
菌寄第８３３８号）の菌株を用いて、実施例２に記載し
たと同様の方法により、６’−ＧＬの粉末１３０ｇを得
た。Example 3 130 g of 6'-GL powder was obtained in the same manner as described in Example 2 using the strain of Trichoderma harzianum SBT 7045 (m1 Research Institute No. 8338).
添付の第１図（ａ及びｂ）は、本発明の方法における微
生物の培養条件と６′−ガラクトシルラクトースの産生
量との間係を示したものであり、第２図は、本発明によ
り培養して得られた培養液中の糖質のＶ＃層クロマトパ
ターンを示したものである。The attached Figures 1 (a and b) show the relationship between the culture conditions of microorganisms and the production amount of 6'-galactosyllactose in the method of the present invention, and Figure 2 shows the relationship between the culture conditions of microorganisms and the amount of 6'-galactosyllactose produced in the method of the present invention. This figure shows the V# layer chromatographic pattern of carbohydrates in the culture solution obtained in this manner.
Claims (4)
ペニシリウム属（Ｐｅｎｉｃｉｌｌｉｕｍ）及びトリコ
デルマ属（Ｔｒ−ｉｃｈｏｄｅｒｍａ）に属する微生物
から成る群から選択される、乳糖から６′−ガラクトシ
ルラクトースの産生能を有する微生物を乳糖を含有する
培地中に培養し、得られた培養物より６′−ガラクトシ
ルラクトースを分離、採取することを特徴とする６′−
ガラクトシルラクトースの製造法。(1) Aspergillus,
A microorganism selected from the group consisting of microorganisms belonging to the genus Penicillium and the genus Trichoderma and having the ability to produce 6'-galactosyllactose from lactose is cultured in a medium containing lactose. 6'-galactosyllactose is separated and collected from the cultured product.
Method for producing galactosyllactose.
ｉｌ−ｌｕｓ ｏｒｙｚａｅ）である特許請求の範囲第
（１）項記載の製造法。(2) The microorganism is Aspergillus oryzae (Aspergillus oryzae).
il-lus oryzae).
−ｉｃｉｌｌｉｕｍ ｒｏｑｕｅｆｏｒｔｉ）である特
許請求の範囲第（１）項記載の製造法。(3) The microorganism is Penicillium rockfuorte (Pen
-icillium roqueforti) according to claim (1).
−ｈｏｄｅｒｍａ ｈａｒｚｉａｎｕｍ）である特許請
求の範囲第（１）項記載の製造法。(4) The microorganism is Trichoderma harzianum (Tric).
-hoderma harzianum).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60219707A JPS6279791A (en) | 1985-10-02 | 1985-10-02 | Production of 6'-galactosyl lactose utilizing lactose-assimilating microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60219707A JPS6279791A (en) | 1985-10-02 | 1985-10-02 | Production of 6'-galactosyl lactose utilizing lactose-assimilating microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6279791A true JPS6279791A (en) | 1987-04-13 |
| JPH0481433B2 JPH0481433B2 (en) | 1992-12-24 |
Family
ID=16739703
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60219707A Granted JPS6279791A (en) | 1985-10-02 | 1985-10-02 | Production of 6'-galactosyl lactose utilizing lactose-assimilating microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6279791A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019009130A1 (en) * | 2017-07-04 | 2019-01-10 | 株式会社ヤクルト本社 | Method for producing galactooligosaccharide |
-
1985
- 1985-10-02 JP JP60219707A patent/JPS6279791A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019009130A1 (en) * | 2017-07-04 | 2019-01-10 | 株式会社ヤクルト本社 | Method for producing galactooligosaccharide |
| JPWO2019009130A1 (en) * | 2017-07-04 | 2020-05-07 | 株式会社ヤクルト本社 | Method for producing galactooligosaccharide |
| US11718841B2 (en) | 2017-07-04 | 2023-08-08 | Kabushiki Kaisha Yakult Honsha | Method for producing galactooligosaccharide |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0481433B2 (en) | 1992-12-24 |
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