JPS6276461A - Method for inspecting blood component and sample for said inspection - Google Patents

Abstract

PURPOSE:To permit automation by coupling an inspection stage for the tangible component in blood and analysis stage for the chemical component in blood and to improve the accuracy of analysis by respectively connecting the inspection stage for the tangible component in blood, blood plasma or serum sepn. stage and analysis stage for the chemical component in blood and functioning the same as a continuous stage. CONSTITUTION:The blood which is added with an anticlotting agent in the 2nd chamber 7, is fed to a blood letting section and is drawn therein is injected into the 1st chamber. A sample vessel 1 contg. the drawn blood in the 1st chamber is successively fed to a blood component inspection line. A specified volume of the sample is drawn from the 1st chamber where the vessel 1 arrives at a morphological inspection section. The blood is fed to, for example, a hematocrit value inspection device, by which the hematocrit value is measured. Further the vessel 1 moves in the blood inspection line and where the vessel arrives at a blood plasma sepn. section, the vessel is set in a sample holder of a centrifugal separator. The centrifugal separator is operated to separate the blood plasma and blood-clot when all the sample vessels are set.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to a method for testing blood'e, its components, and a sample container for the testing, and in particular, to testing of formed components in blood and analysis of chemical components. The present invention relates to a blood component testing method that allows consistent testing of blood components, and a blood component sample container that can be used as a sample container for testing tangible components in blood and as a sample container for analyzing chemical components in blood.

The present invention also provides a method for preparing a sample for a blood component test in which sample collection for a blood test, sample pretreatment, and sample collection for chemical component analysis in blood are all carried out in a container, and a method capable of separating plasma and serum from blood. This invention relates to a sample container for blood component testing that is also used for pretreatment.

(b) Conventional technology Blood circulates throughout the body through body fluids, delivering substances such as oxygen, nutrients, and hormones to the tissues, and carrying away waste products from the tissues. It comprehensively reflects the metabolism of the body's cells and tissues, and if there is a lesion somewhere in the body, it will change accordingly. Therefore, blood tests are used for diagnosing various diseases and determining their disease status and therapeutic effects.

However, there are two types of blood component tests, for example, those that use whole blood as a test sample, such as tests for formed components in blood, and those that use solids such as blood cells, such as analyzes of blood chemistry rIi and minutes. Some tests use plasma or serum from which components have been removed,
Since each test is performed using different equipment, in order to perform a test for blood-formed components and an analysis of chemical components in the same blood, for example, the collected blood must be tested using a sample tI instrument.
A portion of this blood is transferred to a container for testing blood formed components and subjected to a blood formed component test, and the remaining portion is sent to a device for separating solid components such as blood cells, and solid components such as blood cells and plasma or serum are separated. The liquid components such as plasma or serum are separated and placed in a sample container for blood chemical component analysis, and used for analysis of chemical components in the blood.

(c) Problems to be solved by the invention Among these blood component tests, the testing and analysis methods for testing for formed components in blood and for analyzing chemical components in blood are becoming increasingly automated. Although tests and analyzes can now be carried out quickly, the sample pretreatment process that separates plasma or serum from blood has not been fully automated, so sample containers must be moved to separate the formed components in the blood. It was not possible to conduct tests or analyze the chemical components in the blood.

Therefore, conventionally, when conducting a blood component test, the sample is divided into two parts: one for testing the physical components in the blood and the other for analyzing the chemical components in the blood, and each sample is placed in separate containers and separated by separate threads. The sample flow is complicated, the inspection and analysis methods are also complicated, the measurement data processing process is also complicated, and the number of sample containers used is large (which has become a problem). There is.

The present invention addresses the complication of testing and analysis techniques due to sample flow and the use of many sample containers in conventional methods when testing the same blood for physical components and analyzing chemical components in the blood. The purpose is to eliminate all problems such as ``M''.

(d) Means for Solving the Problems The present invention provides - sample containers equipped with a blood sample accommodating part and a plasma or serum separating part from the blood, and for testing formed components from the collected blood. By collecting a sample, then separating plasma and serum from the collected blood, and collecting a specimen for chemical component analysis from the separated serum or plasma, This paper proposes a test method for testing blood ill components and a sample U device for the test.

That is, in the present invention, after a blood sample for a blood test is collected from a sample chamber, the remaining blood in the sample chamber is sent from the sample chamber to a separation chamber under the action of centrifugal force, and then, under the action of centrifugal force. After separating the coagulated components and stopping the centrifugal force, the supernatant is returned from the separation chamber to the sample chamber by its own weight, and a sample of the supernatant is collected from the sample chamber for component analysis. The present invention relates to a method for testing blood components, and the present invention also provides the following features:
It is characterized by comprising a first chamber, a second chamber communicating with the first chamber, an opening located above the first chamber, and a weir located at the boundary between the first chamber and the second chamber. It is in a sample container for blood component testing.

In the present invention, collected blood is stored in a sample chamber, and a blood sample for a blood test is collected from the sample chamber. Thereafter, plasma or serum is separated from the remaining blood in the sample chamber for blood chemical component analysis.In the present invention, centrifugal force due to rotation or revolution is applied to the sample chamber, The remaining blood is sent to a separation chamber by its centrifugal force, and plasma or serum is separated by the centrifugal force. The plasma or serum separated as a supernatant is returned to the sample chamber by its own weight after the centrifugal force is stopped, and a sample for blood chemical component analysis is collected.

Since the hemacrit value of blood is usually 45 to 50%, the separation chamber is placed at a predetermined position or height based on this hemacrit value so that only the supernatant liquid flows from the separation chamber to the sample. It is preferable to provide a communication channel, a weir, etc. because only plasma or serum can be returned.

In the present invention, in order to send the collected blood in the sample chamber to the separation chamber by centrifugal action, the separation chamber is separated from the sample chamber and is formed with a communication path at its opening. In this case, in order to automatically transfer the separated plasma or serum after centrifugation to the sample chamber by gravity, the separation chamber and the communication path from the sample chamber to the separation chamber are connected to the collected blood stored in the sample chamber. Formed above the liquid level.

In the sample container of the present invention, a double container is provided separated by a weir. Among these, chamber - is used as a sample container for whole blood and serum or plasma, and the other chamber functions as a centrifuge tube when preparing serum or plasma from blood. .

Therefore, the second chamber is formed at a right angle or an acute angle with respect to the first chamber so that the separated serum or plasma can be easily transferred to the first chamber.

When the second chamber is connected at right angles or nearly right angles to the first chamber, it is possible to prevent precipitated blood clots from flowing into the first chamber together with the separated serum or plasma when the centrifuge stops operating. A weir member is provided.

However, if the second chamber is connected to the first chamber at an acute angle, the connection between the first and second chambers will function as a weir.
There is no need to provide a special weir member, and the position where the second chamber is provided relative to the first chamber is at a height that allows the liquid in the first chamber to easily move to the second chamber by centrifugal action.

Although the first chamber and the V-second chamber can be made of any suitable material, it is preferable that they be made of a transparent material so that the inside can be seen.

Further, the cross-sectional shapes of the first chamber and the second chamber can be formed into an appropriate shape such as circular or rectangular.

In the second chamber, blood clotting promoters such as blood clotting enzymes in snake venom, heparin, double oxalate, etc. are placed on the chamber wall, as is conventionally used, so that serum or plasma can be easily prepared. Alternatively, it is preferable to attach an anti-blood coagulant such as EDTA by coating or adding.

(E) Function In the present invention, the collected blood is automatically shuttled from the sample chamber to the separation chamber separated from the sample chamber by the action of centrifugal force due to rotation or revolution. Moreover, in the separation chamber, plasma or serum is subsequently separated by the action of this centrifugal force, but by stopping this centrifugal force, the separated plasma or serum can be separated from the sample while leaving the chamber. automatically returned to the room. Thus, for example, plasma, sample or serum samples for analysis of chemical components in blood can be collected from the sample chamber.

Thus, in the present invention, for example, morphological examination,
Collection of blood samples for blood tests such as hemostatic coagulation tests and blood transfusion tests, separation of plasma or serum from blood, and chemical components in blood for clinical chemistry tests, immunological tests, biochemical tests, etc. Sample collection operations for analysis can be performed as a consistent series of steps. Therefore, these inspections and analyzes can also be performed along the line.

The sample container of the present invention has a second chamber dedicated to blood ↑a and plasma separation attached to the first chamber, so that the first chamber can be used as a V vessel for the collected blood, and the collected blood can be automatically transferred by centrifugation or the like. Therefore, the sample container of the present invention can be operated by placing the collected blood directly into the first chamber and placing the sample container into a centrifuge or after placement. Samples for blood tests such as red blood cell count, white blood cell count, hematocrit, red blood cell index, etc. can be taken beforehand. With respect to the remaining blood from which a sample for such a blood test has been collected, plasma or serum can be separated by applying a centrifugal action to the sample container. The plasma or serum thus obtained is subjected to analysis of chemical components in the blood.

Therefore, the sample container of the present invention is moved along the line, and the blood initially collected from the blood collection chamber is collected for testing of the formed components in the blood, such as morphological testing, hemostatic coagulation testing, and blood transfusion testing. etc., to examine the test items, then move to the next step to separate plasma or serum, and then analyze this separated plasma or serum for chemical components in the blood. For example, it can be subjected to clinical chemistry tests, body fluid/electrolyte chamber/acid-base balance tests, immune serum tests, etc., and the respective analysis items can be analyzed.

Furthermore, if two sample containers such as those described above are placed side by side and integrally formed as one sample container, for example, for the same patient's blood, an anticoagulant is added to perform plasma separation, and a coagulant is added to perform serum separation. Plasma and serum samples can be prepared in two sample containers, so inspection and IJ analysis for an extremely wide range of test and analysis items can be carried out on the line where the sample containers move. It can be done inside.

(F) Example Hereinafter, one embodiment of the present invention will be described with reference to the accompanying drawings, but the present invention is not limited in any way by this description and exemplification.

The figure is a schematic side sectional view showing one embodiment of the present invention.

The sample container 1 is integrally formed with a container wall 2.
A part of the leg forms the leg ff1S3. An opening 4 is provided at one end of the top plate of the sample container 1, and the opening 4 is located above the first chamber 5. The first chamber 5 is connected to the second chamber 7 with the weir member 6 as a boundary. The second chamber 7 is located above the first chamber 5, and is located at the bottom of the first chamber 5! lt8 is the second chamber 7
It is formed so that blood in the first chamber 5 can easily move to the second chamber 7 by the action of centrifugal force.

Since the sample container 1 of this example is formed in this way, first, an anticoagulant (I! Then, the collected blood is injected into the first chamber 5.The sample container 1 containing the collected blood in the first chamber is then sent to a blood component test line (not shown).Sample container 1 When the blood reaches the morphological examination department (not shown), a certain amount of blood is collected from the first chamber 4 and sent to, for example, a hematocrit test device (not shown), where the hematocrit value is determined. Furthermore, when the sample container 1 moves through the blood test line and reaches the plasma separation section (not shown), it is loaded into a sample holder (not shown) of a centrifuge. When all the sample containers have been set, the centrifuge is operated to separate plasma and blood clots.When the centrifuge is operated, the blood placed in the first chamber 5 is separated by centrifugal action. , moves over the weir member 6 to the second chamber 7, where the plasma is separated by the action of an anticoagulant.After a certain period of time has elapsed after the operation of the centrifuge, the operation of the centrifuge is stopped and the plasma is separated. Transferring the separated plasma to the first chamber 5 When the operation of the centrifuge is stopped, the separated plasma automatically moves over the weir member 6 to the first chamber 5, but blood clots are removed from the weir. The plasma in the first chamber 5 is dammed by the member 6, so that there is no leakage.

An autopipettor (
Not shown. ), a plasma sample is collected from the first chamber 5 of the sample container 1 into a reaction tube of an automatic biochemical analyzer and analyzed for desired analysis items.

As described above, the sample container 1 of this example can automatically test and analyze a wide range of test items and analysis items I by moving the blood component test line.

(G) Effect of A of the Invention In the present invention, a second chamber exclusively for serum and plasma fraction m is attached to the first chamber, so that the first chamber can be used as a container for the collected blood, and the collected blood cannot be transferred manually. For example, the serum or plasma can be automatically transferred to a container for serum or plasma separation by centrifugation, unlike in conventional blood component tests.
This eliminates the need for manual operations such as dividing the collected blood into two, transferring it from the sample container to the centrifuge tube, and further transferring it to the sample cup, which can shorten analysis work time and make it easier to handle serum or plasma. It is possible to reduce the risk of infection of pathogens, etc. to analysis personnel, etc.

In addition, the present invention eliminates the operation of transferring blood, serum, and plasma from a blood collection container to a sample container or centrifuge tube, etc., thus avoiding human errors caused by mistaken transfer compared to conventional devices. It is possible to improve the reliability of the obtained analytical values.

Furthermore, according to the sample container of the present invention, it is possible to separate plasma or serum from blood in the sample container, so that it is possible to accommodate a blood sample at the time of blood collection, and also to carry out tests for formed components in blood such as blood tests. Test samples or analysis samples can be provided for chemical component analysis in blood such as clinical chemistry tests and serum tests.

As described above, the present invention provides a step for testing formed components in blood,
The plasma or blood 1n separation step and the chemical component analysis step in the blood are connected to each other and act as a continuous process. It not only makes it possible to combine and automate J analysis processes, but also improves analysis accuracy, which has a large impact.

[Brief explanation of drawings]

The figure is a schematic side sectional view showing one embodiment of the sample container of the present invention. In the figure, as for the reference numerals, 1 is a sample container, 2 is a vessel wall, 3 is a leg, 4 is an opening, 5 is a first chamber, 6 is a weir member, 7 is a second chamber, and 8 is a bottom wall. Procedural amendment, document 60.10.17 Showa year, month, day 1, indication of case Patent application 2 filed on September 30, 1985
, Title of the invention Blood component testing method and test sample container 3, Relationship with the amended person's case Name of patent applicant (1,99) Shima t1N Seisakusho Co., Ltd. 4, Agent ~ 5, Date of reasons for refusal Spontaneous request 6, column for scope of patent request for amendment and column for detailed description of invention I-
7. Contents of amendment (1) The claims column will be amended as shown in the attached sheet. (2) The detailed explanation column for Gyomei will be corrected as follows. ■ In the 11th line of page 6 of the specification, the statement ``The sample is returned to the sample chamber by its own weight,'' has been corrected to ``The sample is allowed to flow into the sample chamber by its own weight.'' ■ In the 17th line of page Pt5a of the specification, the statement "It is preferable because only serum can be returned" has been corrected to "It is preferable because only serum can flow in or be returned." ■ The second line of page 7 of the Ming #Ill book says, “You will be automatically dismissed.”
The statement has been amended to read "automatically transferred or replaced." Scope of Patent No. n+'l (1) After a blood sample for blood testing is collected from a sample chamber, the remaining blood in the sample chamber is sent from the sample chamber to a separation chamber by the action of centrifugal force, and then, The coagulated components are separated under the action of centrifugal force, and after the centrifugal force is stopped, the supernatant liquid is caused to flow from the separation chamber into the sample chamber by its own weight, and a sample of the supernatant liquid is collected from the sample chamber for component analysis. A blood component testing method characterized by performing the following. (2) The first chamber, the second chamber communicating with the first chamber, and the first chamber'
A sample container for blood component testing characterized by having a sole opening located above and a weir located at the boundary between a first chamber and a second chamber. (3) Blood component test according to claim 2, characterized in that a blood coagulation promoter, anti-blood e, or coagulant is attached to at least a part of the wall of the second chamber. sample container.

Claims (3)

[Claims] (1) After a blood sample for blood testing has been collected from the sample chamber, the remaining blood in the sample chamber is sent from the sample chamber to the separation chamber by the action of centrifugal force, and then the coagulated components are removed under the action of centrifugal force. A blood component test characterized in that after separating and stopping the centrifugal force, the supernatant is returned by its own weight from the separation chamber to the sample chamber, and the supernatant is sampled from the sample chamber for component analysis. Method. (2) Comprising a first chamber, a second chamber communicating with the first chamber, a unique opening located above the first chamber, and a weir located at the boundary between the first and second chambers. A sample container for blood component testing characterized by: (3) A sample for blood component testing according to claim 2, wherein a blood coagulation promoter or an anticoagulant is attached to at least a part of the wall of the second chamber. container.