JPS6276442A - Capillary type isokinetic electrophoresis device - Google Patents

Abstract

PURPOSE:To permit the continuation of analysis under the determined migration conditions even with a sample which produces a resulted product by the reaction with a terminal liquid by permitting the discharge of the resulted product of reaction near a sample injection port. CONSTITUTION:A change-over member 14 is turned to close the respective holes 12, 13 of a joint member 11 with the inside wall surface of the member 14 after injection of the sample and migration current is supplied, then some sample S induces the reaction with the terminal liquid T and produces the solid matter A formed by the reaction. Only the unreacted material S' in the sample moves to a migration tube 2 side and the solid matter A is left in the terminal liquid T upon lapse of specified time. The member 14 is turned to open a discharge port 13 at this point of the time, then the solid matter A is discharged together with the terminal liquid to the outside of the tube. The sample injection port 12 and discharge port 13 are again closed to continue the migration after the end of the discharge. Then the solid matter no longer exists in the migration tube 2 and the pipe 3 and the optimum migration current flows. The analysis with high accuracy is thus made possible.

Description

[Detailed description of the invention] B. Industrial application fields The present invention relates to a capillary isotachophoresis device.

In a conventional capillary tube type isotachophoresis device, one end of a capillary tube is closed and the other end contains a leading liquid, and one end of a pipe containing a terminal liquid is connected so that both liquids are in contact with each other. Inject the sample into the interface between the two liquids by pushing out the sample, fill the pipe on the terminal liquid side with the sample, and then place the terminal electrode between the leading electrode and the terminal electrode.
It is separated into components by passing through a tube.

By the way, depending on the sample, bubbles and precipitates are generated due to chemical reactions with the terminal liquid, and these bubbles and precipitates flow into the electrophoresis tube during electrophoresis, changing the electrophoresis conditions and causing a decrease in separation accuracy. Problem 6 C. Purpose In view of these problems, the present invention aims to provide a capillary type isotachophoresis device that can separate a sample with high precision regardless of the reaction with the leading liquid. do.

The structure of the twenty-first invention, that is, the feature of the present invention is that the reaction product can be discharged near the sample injection port.

E. Embodiments The details of the present invention will be explained below based on illustrated embodiments.

FIG. 1 shows an embodiment of the present invention, and in FIG. 5, tube number 1 indicates one end of the migration tube 2 containing the leading liquid and 11! the terminal liquid. At the same time as the connection connecting one end of the pipe 3, as shown in FIG. A discharge port 13 is bored on the lower surface side, and a through hole switching part *A14 is provided on the outer periphery of the joint member 11 in an airtight and rotatable manner. The &A holes 15 and 16 that can communicate with the outlet 13 are connected to the hole 12 of the joint member 11.
．． 13 and is bored at a position offset from the arrangement angle θ. In addition, the code 4 in the figure is the leading liquid 4P! 5 and 7 represent a constant voltage circuit that supplies electrophoresis current through a terminal liquid tank 6, and a detector, respectively.

In this embodiment, the through hole switching member 14 is rotated to connect the through hole 15 side to the sample injection port 12 of the indium member 11!
14, the sample outlet 13 of the joint member 11 is turned 1'('!) by the inner peripheral surface of the through hole switching member 14. In this state, the sample injection port 12 and the through hole 1
When the sample is injected through the vibrator 5, the sample S is accommodated on the terminal liquid side of the vibrator 3 while pushing the terminal liquid T (FIG. 3) back to the terminal tank 6 side. After pouring the sample 1, rotate the switching member] 4 to open the 4 holes 12 and 13 of the joint member 11.
When the switching member 14 is closed with the inner wall surface of the switching member 14 and an electrophoretic current is supplied, some of the sample S reacts with the terminal liquid T to produce a reaction product solid A (FIG. 2). In this way, after a certain period of time has elapsed, only the unreacted substance S in the sample moves to the electrophoresis tube 2 side, and the solid substance A produced by one reaction moves to the terminal liquid T.
(Illustration II). At this point, switching member 1
4 to open the discharge port 13, the reaction product solid A is discharged out of the tube together with the terminal liquid. After the discharge is completed,
The sample injection port 12 and discharge port 13 are closed again to continue electrophoresis. As a result, no solid matter is present in the electrophoresis tube 2 and the vibrator 3, and an optimal electrophoresis current flows, allowing highly accurate analysis.

Furthermore, if gas is generated as a reaction product, as soon as the sample injection port 12 side is opened, the joint member 11
Another hole may be drilled in the upper side of the tube, and the liquid may be discharged through this hole.

FIG. 4 shows the second embodiment of the present invention together with its operating state. When a reaction product A is generated after pouring 4 test tubes, an unreacted sample S' is transferred to the electrophoresis tube. At the point when it is moved to the side, the terminal liquid containing vibrator 3 is removed from the migration tube 2 by the vibrator exchange mechanism (122), and replaced with a new vibrator 3' filled with a new terminal liquid T'. (II).

(Effects) As described above, according to the present invention, it is possible to discharge the terminal liquid at a location where the leading liquid and the terminal liquid form an interface.

Even if a sample produces a product due to reaction with the terminal liquid, the reaction product can be quickly removed from the analysis system and analysis can be continued under predetermined electrophoresis conditions.

[Brief explanation of drawings]

FIG. 1 is a configuration diagram showing an embodiment of a device in which the present invention is required, and FIG. FIG. 3 is a perspective exploded view, and FIG. 3 is an explanatory diagram showing the operation of the same device; FIG. ■...Both connections 2...Migration tube 3...
... Terminal vibe 4 ... Joint member 12 ... Sample inlet 13 ... Outlet port 14
...Through hole switching member

Claims (1)

[Claims] A capillary isokinetic electrophoresis device in which a pipe containing a terminal liquid and a pipe containing a leading liquid are connected via a means for extracting the terminal liquid.