JPS627111Y2 - - Google Patents

Description

[Detailed description of the invention] (Technical field) This invention involves sequentially immersing pieces of biological tissue in various chemical solutions in order to make microscopic specimens of biological tissue.
A one-tank embedding device that finally infiltrates molten paraffin and wraps it in solidified paraffin.The one-tank embedding device allows the tank containing the processing chemical to be easily attached to and removed from the embedding device. This invention relates to a chemical tank connection structure.

After the paraffin solidifies, the biological tissue piece wrapped in paraffin is thinly sliced, stretched and mounted on a specimen glass, and subjected to processes such as removing the paraffin, staining, and attaching a cover glass to form a microscopic specimen.

Figure 1 shows an outline of a single-vessel embedding device. A pipe 3 leads to the upper part of the processing tank 2 covered with an airtight lid 1, and one pipe 5 leads to a vacuum pump 6 through a three-way valve 4, and the other leads to the atmosphere through a pipe 7 through an air filter 8. has been done. The pipe 7 may be connected to a discharge port of a compressor or a vacuum pump. A pipe 9 is connected to the bottom of the processing tank 2, and alcohol,
Multiple chemical tanks 1 for formalin, xylene, etc.
3 and 14 are selectively passed through the processing tank 2. The upper space of each chemical liquid tank 13, 14 is communicated with a collecting pipe 17 through pipes 15, 16, and the collecting pipe 17 is connected to a pipe 18.
to the bottom of the water tank 19, and the water tank 1
The upper space of 9 is a pipe 20, another collecting pipe 21, and a pipe 22.
After the air and gas sent through the pipe 18 are washed with water, the gas is adsorbed through the activated carbon layer, and only the air is released into the atmosphere. The collecting pipes 17 and 21 may be constructed by partitioning one collecting pipe with a partition plate 24 as shown in the figure. The inside of the collector cylinder 17 is connected to the exhaust port of the vacuum pump 6 through a pipe 25.

Since the one-tank embedding apparatus is configured as described above, when the three-way valve 4 is operated and the pressure inside the processing tank 2 is reduced by the vacuum pump 6, the chemical solution tank connected to the processing tank 2 by the rotary valve 10, e.g. tank 13
The chemical solution is sucked into the treatment tank 2 and immerses the liquid-permeable cage 26 containing the biological tissue pieces. Exhaust air is sent to fill the space where the chemical solution has decreased. When the chemical liquid treatment for a predetermined time is completed, the three-way valve 4 is switched to send the atmosphere through the air filter 8 to the processing tank 2, and the chemical liquid is passed through the rotary valve 10.
It flows down to the original chemical tank 13 through the tank 1
The air inside 3 is pushed out into the collecting pipe 17, sent to the water tank 19 through the pipe 18 to be washed with water, and further passes through the second collecting pipe 21 and the activated carbon tank 23, where the gas is adsorbed and released into the atmosphere. Ru. By washing with water and treating with activated carbon in this manner, harmful gases and odors emitted from the chemical solution can be collected and the air in the processing chamber can be prevented from becoming contaminated. In this way, the chemical solution is supplied and discharged to and from the processing tank 2 in sequence for each chemical solution tank, and finally, the biological tissue pieces are treated in the same manner with melted paraffin to embed the biological tissue pieces.

In such a one-tank embedding device, if the chemical solution deteriorates or is consumed, the chemical solution tank must be removed from the embedding device and the drug solution must be replaced. Since a large number of chemical liquid tanks are used, as many as ten or so, it is required that these can be quickly attached and detached, but heretofore there has been no suitable connection structure for this purpose.

(Purpose of the invention) The object of this invention is to obtain a chemical tank connection structure in a single-tank embedding device that allows such a chemical tank to be connected quickly.

(Structure of the present invention) The chemical liquid tank connection structure in the one-tank embedding device of the present invention is such that a chemical liquid passage is connected to a rotary valve by an outer pipe fixed to the tank case via a collection tube and a flexible inner pipe. By forming a double air passage and an air passage leading to the collector pipe, when placing the chemical tank in the tank case, the above-mentioned A double passage is connected into the tank to allow the circulation of the chemical solution and the inflow and outflow of air within the tank, and the double passage is separated by the reverse operation so that the chemical solution tank can be removed.

(Embodiment of the present invention) Figure 2 and subsequent figures illustrate the chemical tank connection structure of the present invention.

The chemical liquid tank 27 has an airtight lid 28 for filling the chemical liquid on the top surface, and a connecting part 29 on the front side (left side in the figure).
It is placed on the mounting plate 30a of the tank case 30 provided in the embedding device. The tank 27 is formed horizontally flat as shown in FIG.
Mounting plate 30 to save space for many tanks
In order to keep the position of the tank constant, the mounting plate 30a has a slightly recessed part on which the tank is placed, or a guide plate 43 is placed on the side of the tank.
is fixed, and the mounting plate is slightly raised at the rear of the tank to prevent the tank from moving back and forth. The tank 27 occupies a certain position on the mounting plate 30a in this way.
A collecting pipe 17 having a rectangular cross section is fixed to the outer surface of the side plate 30b of the case in front of the case by leg pieces 17a at both ends, and a receiving part 31 that airtightly penetrates the collecting pipe 17 immediately before each tank is attached to the collecting pipe 17. In addition to being fixed, the receiving part 31 can be inserted into the connecting part 29 of the tank by passing through the side plate 30b of the case.

The receiving portion 31 is constructed as shown in FIG. That is, the outer tube 32, which has an outer flange 32a and has a side hole 32b, is inserted into the collecting pipe 17 from the right side (left and right refers to FIG. 4. The same applies hereinafter), and the hollow screw 33 is screwed in from the left side. Combine outer tube 3
2. Fix the hollow screw 33 to the collecting pipe 17. 3
4 and 34 are packings for preventing leakage from the collecting pipe 17; and 35 are packings for preventing the outer tube 32 from rotating during the above-mentioned screwing. This is a fastener fixed to 17. A flexible inner tube 37 having an outer diameter smaller than the inner diameter of the outer tube 32 is inserted into the hollow screw 33 while keeping it airtight with an O-ring 36. Inner pipe 37
is connected to the pipe 11 or 12 shown in FIG. 1 by a fitting 42.

The connecting portion 29 of the chemical solution tank 27 is constructed by screwing a perforated cap 39 into a vertical wall 38 surrounding an opening on the front side of the tank to secure the outer flange 40a of the receiver 40. A packing 41 is interposed between the outer flange 40a and the end face of the vertical wall 38 for airtightness. An inner flange 40b is formed on the inner surface of the receiver 40. The receiver 40 and the outer tube 32 are connected to the inner flange 4
Air in the tank 27 from between 0b and the outer pipe 32,
Each is made of semi-rigid synthetic resin to prevent gas from leaking, and has a packing action for elastic connection.

With the above structure, the receiving part 31 is held in the tank case 30 via the collector pipe 17, and the outer pipe 32 and the inner pipe 37 are made to protrude into the case.

To place the tank 27 filled with a chemical solution in a predetermined position inside the case outside the case, first insert the long flexible inner tube 37 into the tank through the cap 39 and the holder 40, and then insert the outer tube 32 into the holder. 40 and is pushed into the case until it touches the side plate 30b of the tank and placed in a predetermined position, the receiving part 31 and the connecting part 29 are connected as shown in FIG. At this time, the inner tube 37 has reached the bottom of the chemical liquid tank. The space above the tank passes through the gap between the inner pipe 37, the receiver 40, and the outer pipe 32, and communicates with the collector pipe 17 through the side hole 32b, and the outer pipe 3 for elastic contact between the outer pipe 32 and the inner flange 40b.
No leakage outside of 2. The pipe 11 or 12 leading to the rotary valve 10 is connected to the inner pipe 3 via a connecting fitting 42.
7 and is fixed to the tank case 30 by the collector pipe 17. Therefore, when the connecting portion 29 of the chemical liquid tank 27 is fitted to the receiving portion 31 as described above, the chemical liquid in the tank is transferred to the rotary valve 10. The air in the tank is sucked into and discharged from the treatment tank 2 through the tank 2, and the air in the tank flows in and out of the collecting pipe 17, so the chemical solution can freely flow in and out of the tank without dissipating harmful vapors emitted from the chemical solution into the work room. You can make them do it.

(Effects of the present invention) As can be seen from the above embodiments, according to the chemical solution tank connection structure of this invention, the tank can be connected while the connecting part 29 attached to the tank is fitted into the receiving part 31 fixed to the tank case 30. When placed in a predetermined position within the case, communication and isolation of the chemical solution and gas passages are automatically performed, allowing for efficient supply and discharge of the chemical solution to and from the processing tank 2. The embedded device can be operated easily and quickly.

[Brief explanation of the drawing]

Fig. 1 is a schematic diagram explaining the outline of a single-tank embedding device, Fig. 2 and the following show embodiments of the present invention, Fig. 2 is a side view showing the external appearance, Fig. 3 is a plan view, and Fig. 4 is a side view showing the external appearance. The figure is an enlarged sectional view taken along the line AA in FIG. 3. 1...airtight lid, 2...processing tank, 3...pipe, 4...
...Three-way valve, 5...Pipe, 6...Vacuum pump, 7...
Pipe, 8... air filter, 9... pipe, 10... rotary valve, 11, 12... pipe, 13, 14... chemical tank, 15, 16... pipe, 17... collecting pipe,
17a...Leg piece, 18...Pipe, 19...Water tank, 20...Pipe, 21...Collection pipe, 22...Pipe,
23... activated carbon tank, 24... partition plate, 25...
Pipe, 26...basket, 27...chemical tank, 28...
Airtight lid, 29...connection section, 30...tank case, 30a...mounting plate, 30b...side plate, 31...
...receiving part, 32...outer tube, 32a...outer flange,
32b... Side hole, 33... Hollow screw, 34... Packing, 35... Fastener, 36... O ring,
37... Inner pipe, 38... Standing wall, 39... Cap, 40... Receiver, 40a... Outer flange, 40
b...Inner flange, 41...Packing, 42...
...Connection fittings, 43...Information board.

Claims (1)

[Scope of utility model registration request] Connecting the outer tube 32 and the hollow screw 33 by airtightly sandwiching the two parallel sides of the collecting pipe 17 attached to the side plate 30b of the tank case 30 and passing through both sides.
The outer pipe 32 and the inside of the collector pipe 17 are connected to each other through the side hole 32 of the outer pipe 32.
b, and the flexible inner tube 37, which has an outer diameter smaller than the inner diameter of the outer tube 32 and communicates with the rotary valve of the single-vessel embedding device, is airtightly connected to the inner surface of the hollow screw 33 to form a hollow screw. 33 and the outer tube 32,
A perforated cap 39 is screwed onto a standing wall 38 that surrounds a hole provided on the side surface facing the outer pipe 32 and inner pipe 37 of the chemical solution tank 27 placed at a predetermined position on the mounting plate 30a of the tank case 30. 38 and cap 39
The outer flange 40a of the receiver 40 is connected to the vertical wall 38 between
The holder 40 has an inner flange 40b which has a gap with the inner tube 37 and comes into elastic contact with the outer surface of the outer tube 32. chemical tank connection structure.