JPS6263863A - Blood-substituting fluid for research of artificial organ or the like - Google Patents

Abstract

PURPOSE:To obtain an inexpensive blood-substituting fluid by mixing microcapsules contg. fluid such as physiological salt soln. or glycerol as a core material with Neuton fluid such as physiological salt soln. or glycerol soln. CONSTITUTION:The microcapsules in which the physiological salt soln. is included, have approximately the same size as the size of the red blood cells in blood and have 7-8 micron average particle size are mixed with the physiological salt soln. which is the Neuton fluid at 45% similar to the volumetric ratio of the blood cells. The microcapsules are formed by using a synthetic resin material such as, for example, vinyl chloride, as a film and including the physiological salt soln. thereon. The microcapsules are formed to disk-shaped flat bodies by heating, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to a blood substitute fluid for research on artificial organs and blood flow in living bodies.

BACKGROUND OF THE INVENTION In recent years, artificial organs such as artificial hearts, artificial blood vessels, and blood pumps have been actively developed and are steadily being put into practical use.

In developing and researching these artificial organs,
Operation and function tests are conducted using physiological saline and the blood of animals such as cows.

Problems to be Solved by the Invention However, physiological saline is a Newtonian fluid and has completely different fluid characteristics from blood, which is a non-Newtonian fluid, and it is completely impossible to simulate blood cell destruction. In addition, although animal blood is similar to human blood, it has problems such as putrefaction and deterioration, so it must be laced with drugs and must be specially preserved at low temperatures or frozen, which limits the number of places where it can be handled. It is something that will be done. In addition, fluid characteristics and blood cell strength vary greatly depending on the animal (individual individual, time elapsed since blood collection, etc.), making it difficult to collect reproducible standard data.

Means for Solving the Problems The present invention was made in view of the above points, and uses physiological saline,
Microcapsules with a diameter of several microns to a dozen microns are encapsulated with cheap, easy-to-handle, and easily-obtained Newtonian fluids such as glycerin solutions, physiological saline, and glycerin solutions. At the same time, the volume ratio of the above-mentioned two-ton fluid and microcapsules is adjusted to the plasma water in blood in order to obtain fluid characteristics that are the same or similar to blood for the development of artificial devices, research on operating characteristics, blood flow, etc. It can be mixed and formed with almost the same volume ratio as blood cells, and the required fluid etc. can be easily obtained and easily produced.
In addition, it is easy to handle, is a non-Newtonian fluid similar to blood, has standard fluid characteristics as a substitute for blood, and can simulate blood cell destruction, greatly promoting the development and research of artificial organs. The purpose is to provide blood substitute fluids for research purposes.

Example Hereinafter, the present invention will be explained based on examples.

First Embodiment An embodiment of the blood substitute fluid of the present invention is produced by mixing Newtonian fluid of physiological saline with microcapsules encapsulating physiological saline. Physiological saline is a Newtonian fluid made into an isotonic solution for normal medical use, and the average particle diameter of this Newtonian fluid saline is approximately the same size as red blood cells in blood containing physiological saline. Microcapsules of ~8 microns are mixed at a volume ratio of approximately 45%, which is similar to the volume ratio of plasma water and blood cells in blood. Microcapsules are coated with a synthetic resin material such as vinyl chloride and encapsulate physiological saline. Then, the microcapsules are made into disk-shaped flat bodies by heating, etc., and the mixture is filtered through a filter with a predetermined mesh size, resulting in granular bodies with a diameter of 7 to 8 microns similar to the above-mentioned red blood cells. It is.

If the granules thus produced are mixed with physiological saline at a volume ratio of 45%, a desired blood substitute fluid can be produced.

Function: If the blood substitute fluid produced as described above is heated to a predetermined temperature and used in an artificial heart device or an artificial heart-lung device, the blood substitute fluid will be able to maintain the state of plasma water and red blood cells, which are the main parts of blood. Because of this configuration, it is possible to obtain fluid characteristics extremely similar to blood, which is a non-Newtonian fluid, making it possible to accurately perform simulation tests on artificial heart devices and artificial heart-lung devices, and to evaluate the operating status and functions of artificial hearts and blood pumps. Accurately reproducible standard data with no variation in characteristics can be collected. Furthermore, by measuring the amount of microcapsule destruction using a microscope or the like after the above simulation test, it is possible to quantify and measure the destruction of blood cells caused by an artificial heart or blood pump.

In addition, it has become possible to visualize flow distribution in artificial hearts and blood pumps, flow distribution in artificial blood vessels, stagnation, etc., and to easily collect reproducible and detailed data. , it is possible to accurately improve the functionality of artificial organs from the viewpoint of fluid dynamics.

In addition, as described in the above examples, the constituent fluids and materials are inexpensive and easily available, are not difficult to manufacture, and are extremely easy to handle, so simulation tests of artificial organs have been carried out extensively. It can be performed by people in the legal field, making it possible to conduct a wide range of research on artificial organs, and the test data is easy to compare and study, unlike those using animal blood such as cows.

Second Example In this example, microcapsules encapsulating physiological saline in which a certain amount of sucrose is uniformly dissolved are produced and mixed with physiological saline at a predetermined volume ratio in the same manner as above to obtain a blood substitute fluid. It constitutes.

In this example, the amount of microcapsule damage caused by an artificial heart or blood pump can be calculated by measuring the concentration of sucrose that has flowed into the blood substitute fluid. In particular, it is preferable because the destruction rate of microcapsules can be measured stationarily using a densitometer.

Note that glucose or other sugars can be dissolved instead of sucrose, and the state of destruction can also be measured by chromaticity, etc. by encapsulating a dye substance with a pigment in microcapsules in the same way as above. .

Third Embodiment In this embodiment, an oily substance such as silicone oil is encapsulated to form a microcapsule.

In this example, it is easy to observe the adhesion of the oily substance flowing out from the destroyed microcapsules to the wall surface of the channel of an artificial organ, etc., and it is possible to measure the growth of a thrombus.

Other Examples In the above examples, physiological saline was used as the fluid for mixing the microcapsules, but it is also possible to use other Newtonian fluids such as glycerin solutions that are available at low cost.
In addition, the mixing ratio with microcapsules is preferably approximately 45% of the ratio of blood plasma water to blood cells, but it may be varied as necessary from a few percent to 90% for purposes such as research on abnormal conditions. be.

Furthermore, although the microcapsules were formed into a disc shape that is the same as the shape of red blood cells that make up the majority of blood cells, they can also be formed into simple spherical shapes.

Furthermore, coating materials for microcapsules include vinylidene chloride, polyethylene, polypropylene, nylon,
Synthetic resin materials such as silicone and polymeric materials such as gelatin can also be encapsulated in the same manner as above, and known chemical, physical,
This can be done using mechanical equipment.

Although the artificial heart device, the heart-lung machine, and the artificial blood vessel have been described as artificial organs, the present invention can also be used for the development and research of artificial organs such as an artificial kidney and an artificial liver.

Effects of the Invention As described above, the present invention allows the necessary fluids to be easily obtained and produced, does not require special storage such as low-temperature storage or frozen storage, and is easy to handle. It is possible to obtain standard fluid properties for non-Ninton fluids similar to blood, and it is also possible to accurately collect simulation data such as destruction of blood cells by artificial devices and blood flow distribution.
This will further promote the development and research of artificial organs.

Claims (3)

[Claims] (1) Cheap, easy-to-handle, easily-obtained Newtonian fluids such as physiological saline and glycerin solutions, and core materials of several microns in diameter using inexpensive, easy-to-handle, and easily obtainable fluids such as physiological saline and glycerin solutions. In addition to mixing microcapsules of about 10-10 microns in size, the volume ratio of the Newtonian fluid and microcapsules is determined in order to obtain fluid characteristics that are the same or similar to blood for research on the development of artificial organs, operating characteristics, blood flow, etc. A blood substitute fluid for artificial organ research, etc., characterized by being formed by mixing plasma water and blood cells in a volume ratio that is almost the same as in blood. (2) A blood substitute fluid for use in artificial organ research, etc., as set forth in claim 1, wherein microcapsules are formed into disk-shaped flat bodies like the shape of red blood cells. (3) The fluid encapsulated in microcapsules to measure the destructive characteristics of blood cells through the use of artificial organs may be different from the Newtonian fluid to be mixed, or even if mixed with the Newtonian fluid, the concentration, chromaticity, weight, etc. A blood substitute for artificial organ research, etc., as set forth in claim 1 or 2, which is loaded with sugar, dye, oil, reaction liquid, etc. so that the degree of destruction of blood cells can be measured by analysis. fluid.