JPS6249584B2 - - Google Patents
Info
- Publication number
- JPS6249584B2 JPS6249584B2 JP52073055A JP7305577A JPS6249584B2 JP S6249584 B2 JPS6249584 B2 JP S6249584B2 JP 52073055 A JP52073055 A JP 52073055A JP 7305577 A JP7305577 A JP 7305577A JP S6249584 B2 JPS6249584 B2 JP S6249584B2
- Authority
- JP
- Japan
- Prior art keywords
- solution
- silicone
- acid
- test piece
- amino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 229920001296 polysiloxane Polymers 0.000 claims description 15
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 claims description 11
- 229940083618 sodium nitroprusside Drugs 0.000 claims description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 10
- WDJHALXBUFZDSR-UHFFFAOYSA-N Acetoacetic acid Natural products CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 claims description 8
- WOWBFOBYOAGEEA-UHFFFAOYSA-N diafenthiuron Chemical compound CC(C)C1=C(NC(=S)NC(C)(C)C)C(C(C)C)=CC(OC=2C=CC=CC=2)=C1 WOWBFOBYOAGEEA-UHFFFAOYSA-N 0.000 claims description 8
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 150000007524 organic acids Chemical class 0.000 claims description 7
- 239000003960 organic solvent Substances 0.000 claims description 6
- 125000003545 alkoxy group Chemical group 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 150000002367 halogens Chemical class 0.000 claims description 3
- 125000005395 methacrylic acid group Chemical group 0.000 claims description 3
- 239000012928 buffer substance Substances 0.000 claims description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 10
- 239000001488 sodium phosphate Substances 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 150000002576 ketones Chemical class 0.000 description 8
- 239000004471 Glycine Substances 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 210000002700 urine Anatomy 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- YEESUBCSWGVPCE-UHFFFAOYSA-N azanylidyneoxidanium iron(2+) pentacyanide Chemical class [Fe++].[C-]#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.N#[O+] YEESUBCSWGVPCE-UHFFFAOYSA-N 0.000 description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 5
- 229910000397 disodium phosphate Inorganic materials 0.000 description 5
- 235000019800 disodium phosphate Nutrition 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 5
- 229910000406 trisodium phosphate Inorganic materials 0.000 description 5
- 235000019801 trisodium phosphate Nutrition 0.000 description 5
- CYIDZMCFTVVTJO-UHFFFAOYSA-N pyromellitic acid Chemical compound OC(=O)C1=CC(C(O)=O)=C(C(O)=O)C=C1C(O)=O CYIDZMCFTVVTJO-UHFFFAOYSA-N 0.000 description 4
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 229920013822 aminosilicone Polymers 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 208000037157 Azotemia Diseases 0.000 description 1
- 208000007976 Ketosis Diseases 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 239000004349 Polyvinylpyrrolidone-vinyl acetate copolymer Substances 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 239000006087 Silane Coupling Agent Substances 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- -1 alcohol amines Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001642 boronic acid derivatives Chemical class 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000004140 ketosis Effects 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 235000019448 polyvinylpyrrolidone-vinyl acetate copolymer Nutrition 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 150000003462 sulfoxides Chemical class 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 208000009852 uremia Diseases 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Description
本発明は体液、殊に尿中のアセト酢酸またはア
セトン（以下、「ケトン体」という）を検出する
ための試験片に関するものである。
糖尿病、腎不全、消化器疾患、尿毒症等により
ケトージスが生起したアセト酢酸、アセトン等の
ケトン体が血液中に増加し尿中に大量に排泄され
る。従つて、尿中のケトン体を測定することによ
つて上記の如き各種疾患の有無の判定あるいは予
後の判定をすることができる。
従来、体液中のケトン体を検出するため種々の
試薬や方法が提案されたが、その多くはニトロプ
ルシド塩を反応試薬として含有する試験片あるい
は組成物をアルカリ性条件下でケトン体と反応さ
せるものであつた。しかしニトロプルシド塩はPH
７以下でのみ安定でPH８以上では分解してしまう
が、ニトロプルシド塩をケトン体検出のために使
用するにはPH８以上のアルカリ性媒体中でないと
作用しないので試験紙上でアルカリ性物質から保
護することが必要となり、これを解決するために
ポリビニルピロリドン酢酸ビニル共重合体、メチ
ルエチルエーテル−無水マレイン酸共重合体等の
有機フイルム形成物質によりニトロプルシド塩を
保護する方法やアルカリ性物質にアミン、アミノ
酸、アルコールアミン等を使用してニトロプルシ
ド塩を安定化する方法が知られているが、これら
は耐湿性、耐熱性が悪い。
本発明は上記事情を鑑みなされたもので、その
目的とするところは湿気、アルカリ性物質に対し
て安定でしかも検出感度のよい製造の容易なケト
ン体検出用試験片を提供することにある。
上記目的を達成するものは、アルカリ性緩衝物
質および水溶性アミノ酸の乾燥物を含む吸水性担
体に、シリコーンを介してニトロプルシドナトリ
ウムおよび有機溶媒に可溶な有機酸の乾燥物を設
けたアセト酢酸またはアセトン検出用試験片であ
る。また、前記シリコーンが、アルコキシ系、ア
ミノ系、ハロゲン系、メタクリル系シリコーンで
あることが好ましい。
本発明に係る試験片は以下のような方法によつ
て製造することができる。まず、紙、ガラス繊
維紙等の吸収性担体をアルカリ性緩衝剤及びアミ
ノ酸溶液に浸し乾燥する。第２にこうして処理し
た担体をシリコーン溶液に浸し、さらにニトロプ
ルシドナトリウム及び有機溶媒に可溶な有機酸の
溶液に浸し乾燥して作製する。第１の処理に使用
される緩衝剤はPH８〜10を与えるものでとくに有
用なものとしてはリン酸三ナトリウム、リン酸二
ナトリウム、ホウ酸塩、炭酸塩エチレンジアミン
四酢酸等で、アミノ酸としては水溶性アミノ酸で
あれば何でもよいが好ましくはグリシンあるいは
アラニンである。その際、含浸液100ml中緩衝剤
は20〜40ｇ、アミノ酸は10〜20ｇを含有するのが
適当である。
第２の処理をしない、すなわちシリコーン処理
をしない試験片は、多湿でない地域で使用する場
合は、充分使用が可能であるが、多湿地域で使用
する場合は、耐湿性に問題がある。シリコーン
は、湿気に対して有効に作用するもので、アルコ
キシ系、アミノ系、ハロゲン系、メタクリル系な
どのシランカツプリング剤が好ましい。例えば、
アミノ系シリコーン、アルコキシシラン系シリコ
ーンなどが好適に使用できる。
さらに、本発明では有機溶媒に可溶な有機酸を
使用することにより、貯蔵中、ニトロプルシドナ
トリウムをアルカリ性物質の有害作用から保護す
ることが可能となつた。有機溶媒に可溶な有機酸
のうちでも特に塩基度が３以上の物質、例えばク
エン酸、エチレンジアミン四酢酸、ピロメリツト
酸などが有効である。
ニトロプルシドナトリウムと有機酸は有機溶
剤、例えばメタノール、ジメチルスルホキシド、
エタノール、ジメチルホルムアミド及びその混合
物に溶かして用いるが、含浸液100ml中ニトロプ
ルシドナトリウムは１〜５ｇ、有機酸は0.5〜５
ｇを含有するのが適当である。
本発明に係る試験片は、ケトン体特に尿中アセ
ト酢酸濃度５mg％以上で紫色に呈色、陽性呈色反
応は15秒以内におこる。そして、アセト酢酸を含
まない正常尿では、何ら変色せず試験片そのもの
の色である微かつ色のままである。
一般的貯蔵の室温状態（温度10〜20℃、湿度20
〜50％）では、いずれも良好な安定性を保持でき
る。
以下、実施例をもつて本発明を詳細に説明す
る。
実施例 １
溶液
リン酸三ナトリウム・12H2O 10ｇ
リン酸二ナトリウム・12H2O 10ｇ
グリシン 10ｇ
蒸留水 50ml
溶液
ヘキサアルキルシラザン系シリコーン ２ｇ
クロロホルム ８ml
溶液
ニトロプルシドナトリウム 0.1ｇ
クエン酸・1H2O 0.08ｇ
ジメチルスルホキシド 10ml
紙に溶液を含浸し、100℃で乾燥する。乾
燥後、溶液を含浸し、さらに溶液を含浸し80
℃で乾燥する。この試験片で、尿100ml中のアセ
ト酢酸５mgを15秒以内に紫色の呈色によつて検出
することができた。また、正常尿では何ら変色せ
ず試験片のそのものの色である微かつ色のままで
あつた。そして一般的貯蔵で変色しない試験片が
得られる。
実施例 ２
溶液
リン酸三ナトリウム・12H2O 10ｇ
リン酸二ナトリウム・12H2O 10ｇ
グリシン 10ｇ
蒸留水 50ml
溶液
アミノ系シリコーン １ｇ
メタノール 100ml
溶液
ニトロプルシドナトリウム 0.1ｇ
ピロメリツト酸 0.05ｇ
エタノール ５ml
ジメチルスルホキシド ５ml
実施例 ３
溶液
リン酸三ナトリウム・12H2O 10ｇ
リン酸二ナトリウム・12H2O 10ｇ
グリシン 10ｇ
蒸留水 50ml
溶液
アルコキシシラン系シリコーン １ｇ
水 100ml
溶液
ニトロプルシドナトリウム 0.08ｇ
マレイン酸 0.13ｇ
ジメチルスルホキシド ５ml
エタノール ５ml
実施例 ４
溶液
エチレンジアミンテトラ酢酸四ナトリウム塩
1.5ｇ
グリシン １ｇ
蒸留水 ５ml
溶液
アルコキシシラン系シリコーン １ｇ
メタノール 100ml
溶液
ニトロプルシドナトリウム 0.1ｇ
クエン酸 0.1ｇ
ジメチルスルホキシド ５ml
エタノール ７ml
上記実施例２〜４による試験片の製造は、実施
例１に記載の方法で行なう。検出感度、安定性に
おいて実施例１と同程度の試験片が得られる。
そして、本願発明の試験片の耐熱性、耐湿性試
験の結果を以下の比較例とともに記載する。
比較例
溶液Ａ
リン酸三ナトリウム・12H2O 10ｇ
リン酸二ナトリウム・12H2O 10ｇ
グリシン 10ｇ
蒸留水 50ml
溶液Ｂ
ニトロプルシドナトリウム 0.1ｇ
ジメチルスルホキシド 10ml
紙に溶液Ａを含浸し、100℃で乾燥する。乾
燥後、溶液Ｂを含浸し80℃で乾燥させたものを比
較例とする。
尚、耐熱性試験は、37℃オーブン中に放置して
行い、耐湿性試験は室温25℃、湿度70％に放置し
て行つた。
The present invention relates to a test piece for detecting acetoacetic acid or acetone (hereinafter referred to as "ketone bodies") in body fluids, particularly in urine. Ketone bodies such as acetoacetic acid and acetone, which are caused by ketosis due to diabetes, renal failure, digestive disorders, uremia, etc., increase in the blood and are excreted in large quantities in the urine. Therefore, by measuring ketone bodies in urine, it is possible to determine the presence or absence of various diseases as described above or to determine the prognosis. In the past, various reagents and methods have been proposed for detecting ketone bodies in body fluids, but most of them involve reacting test pieces or compositions containing nitroprusside salts as a reaction reagent with ketone bodies under alkaline conditions. It was hot. However, nitroprusside salt has a PH
It is stable only at pH 7 or lower and decomposes at pH 8 or higher, but when using nitroprusside salt to detect ketone bodies, it only works in an alkaline medium with pH 8 or higher, so it is necessary to protect it from alkaline substances on the test paper. To solve this problem, the nitroprusside salt is protected by organic film-forming substances such as polyvinylpyrrolidone-vinyl acetate copolymer and methyl ethyl ether-maleic anhydride copolymer, and alkaline substances such as amines, amino acids, alcohol amines, etc. It is known that nitroprusside salts are stabilized using The present invention was made in view of the above circumstances, and its purpose is to provide a test piece for detecting ketone bodies that is stable against moisture and alkaline substances, has good detection sensitivity, and is easy to manufacture. What achieves the above purpose is acetoacetic acid or acetone, in which sodium nitroprusside and a dry organic acid soluble in an organic solvent are provided on a water-absorbing carrier containing an alkaline buffer substance and a dry water-soluble amino acid via silicone. This is a test piece for detection. Further, it is preferable that the silicone is an alkoxy silicone, an amino silicone, a halogen silicone, or a methacrylic silicone. The test piece according to the present invention can be manufactured by the following method. First, an absorbent carrier such as paper or glass fiber paper is soaked in an alkaline buffer and an amino acid solution and dried. Second, the thus treated carrier is immersed in a silicone solution, further immersed in a solution of sodium nitroprusside and an organic acid soluble in an organic solvent, and dried. The buffer used in the first treatment is one that gives a pH of 8 to 10, and particularly useful ones include trisodium phosphate, disodium phosphate, borates, carbonates, ethylenediaminetetraacetic acid, etc., and amino acids are water-soluble. Any amino acid may be used, but glycine or alanine is preferred. In this case, it is appropriate that 100 ml of the impregnating solution contain 20 to 40 g of the buffer and 10 to 20 g of the amino acid. A test piece that is not subjected to the second treatment, that is, without silicone treatment, can be used satisfactorily when used in a region that is not humid, but has a problem in moisture resistance when used in a humid region. The silicone has an effective effect on moisture, and is preferably an alkoxy-based, amino-based, halogen-based, or methacrylic-based silane coupling agent. for example,
Amino silicones, alkoxysilane silicones, and the like can be suitably used. Furthermore, in the present invention, by using an organic acid soluble in an organic solvent, it has become possible to protect sodium nitroprusside from the harmful effects of alkaline substances during storage. Among organic acids soluble in organic solvents, substances with basicity of 3 or more, such as citric acid, ethylenediaminetetraacetic acid, and pyromellitic acid, are particularly effective. Sodium nitroprusside and the organic acid are mixed in an organic solvent such as methanol, dimethyl sulfoxide,
It is used by dissolving it in ethanol, dimethylformamide, or a mixture thereof. Sodium nitroprusside is 1 to 5 g, and organic acid is 0.5 to 5 g in 100 ml of impregnating solution.
It is appropriate to contain g. The test piece according to the present invention turns purple when the concentration of ketone bodies, especially urinary acetoacetic acid, is 5 mg% or more, and a positive color reaction occurs within 15 seconds. In addition, normal urine that does not contain acetoacetic acid does not change color at all and remains the same faint color as the test piece itself. General storage room temperature conditions (temperature 10~20℃, humidity 20℃)
~50%), all can maintain good stability. Hereinafter, the present invention will be explained in detail with reference to Examples. Example 1 Trisodium phosphate solution 12H 2 O 10g Disodium phosphate 12H 2 O 10g Glycine 10g Distilled water 50ml Solution Hexaalkylsilazane silicone 2g Chloroform 8ml Solution Sodium nitroprusside 0.1g Citric acid 1H 2 O 0.08g Dimethyl Impregnate 10 ml of sulfoxide paper with the solution and dry at 100℃. After drying, impregnate with solution and further impregnate with solution 80
Dry at °C. With this test strip, 5 mg of acetoacetic acid in 100 ml of urine could be detected within 15 seconds by purple coloration. In addition, normal urine did not change color at all and remained the same faint color as the test piece itself. A test piece that does not discolor during general storage is obtained. Example 2 Trisodium phosphate solution 12H 2 O 10g Disodium phosphate 12H 2 O 10g Glycine 10g Distilled water 50ml Solution Amino silicone 1g Methanol 100ml Solution Sodium nitroprusside 0.1g Pyromellitic acid 0.05g Ethanol 5ml Dimethyl sulfoxide 5ml Example 3 Trisodium phosphate solution 12H 2 O 10g Disodium phosphate 12H 2 O 10g Glycine 10g Distilled water 50ml Solution Alkoxysilane silicone 1g Water 100ml Solution Sodium nitroprusside 0.08g Maleic acid 0.13g Dimethyl sulfoxide 5ml Ethanol 5ml Example 4 Solution ethylenediaminetetraacetic acid tetrasodium salt
1.5g Glycine 1g Distilled water 5ml Solution alkoxysilane silicone 1g Methanol 100ml Solution Sodium nitroprusside 0.1g Citric acid 0.1g Dimethyl sulfoxide 5ml Ethanol 7ml The test pieces according to Examples 2 to 4 above were manufactured by the method described in Example 1. Let's do it. A test piece with detection sensitivity and stability comparable to that of Example 1 was obtained. The results of the heat resistance and moisture resistance tests of the test pieces of the present invention will be described together with the following comparative examples. Comparative Example Solution A Trisodium phosphate 12H 2 O 10g Disodium phosphate 12H 2 O 10g Glycine 10g Distilled water 50ml Solution B Sodium nitroprusside 0.1g Dimethyl sulfoxide 10ml Paper is impregnated with Solution A and dried at 100°C. After drying, the sample was impregnated with solution B and dried at 80°C as a comparative example. The heat resistance test was performed by leaving the product in an oven at 37°C, and the moisture resistance test was performed by leaving the product at a room temperature of 25°C and a humidity of 70%.
【表】【table】
Claims (1)
乾燥物を含む吸水性担体に、シリコーンを介して
ニトロプルシドナトリウムおよび有機溶媒に可溶
な有機酸の乾燥物を設けたことを特徴とするアセ
ト酢酸またはアセトン検出用試験片。 ２ 前記シリコーンが、アルコキシ系、アミノ
系、ハロゲン系、メタクリル系シリコーンである
特許請求の範囲第１項に記載のアセト酢酸または
アセトン検出用試験片。[Scope of Claims] 1. A water-absorbing carrier containing an alkaline buffer substance and a dried water-soluble amino acid is provided with sodium nitroprusside and a dried organic acid soluble in an organic solvent via silicone. Test piece for detecting acetoacetic acid or acetone. 2. The test piece for detecting acetoacetic acid or acetone according to claim 1, wherein the silicone is an alkoxy-based, amino-based, halogen-based, or methacrylic silicone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7305577A JPS547993A (en) | 1977-06-20 | 1977-06-20 | Test piece for detecting keton member |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7305577A JPS547993A (en) | 1977-06-20 | 1977-06-20 | Test piece for detecting keton member |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3205082A Division JPS57211067A (en) | 1982-03-01 | 1982-03-01 | Test specimen for detection of ketone body |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS547993A JPS547993A (en) | 1979-01-20 |
| JPS6249584B2 true JPS6249584B2 (en) | 1987-10-20 |
Family
ID=13507281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7305577A Granted JPS547993A (en) | 1977-06-20 | 1977-06-20 | Test piece for detecting keton member |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS547993A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2733426C3 (en) * | 1977-07-23 | 1981-08-06 | Behringwerke Ag, 3550 Marburg | Diagnostic agent for the detection of ketone bodies in liquids and process for its manufacture |
| JPH01224667A (en) * | 1988-03-03 | 1989-09-07 | Kokusai Shiyaku Kk | Appliance for detecting ketone body |
-
1977
- 1977-06-20 JP JP7305577A patent/JPS547993A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS547993A (en) | 1979-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3406015A (en) | Means for detecting the fertile period | |
| JPS6412343B2 (en) | ||
| US3585004A (en) | Test composition and device for detecting couplable compounds | |
| JPS6250782B2 (en) | ||
| JP3631300B2 (en) | Creatinine test method | |
| Bin Chen et al. | Determination of allantoin in biological, cosmetic, and pharmaceutical samples | |
| CN101000309A (en) | Test paper for quickly investigating formol content in aquatic product | |
| JPH0511908B2 (en) | ||
| US4440724A (en) | Composition for detecting ketone bodies and method of preparation | |
| US3447905A (en) | Test formulation and device and method for creating an acid ph environment therein | |
| NO742321L (en) | ||
| JPH09145711A (en) | Specimen to detect protein in urine | |
| US4184850A (en) | Diagnostic agent for the detection of ketone bodies in fluids and process for its manufacture | |
| Berry et al. | Detection of metabolic disorders: Chromatographic procedures and interpretation of results | |
| JPS6249584B2 (en) | ||
| JPH0532040B2 (en) | ||
| US20030170905A1 (en) | Diagnostic beads for the detection of blood in animal excreta and a method for production thereof | |
| JPH11153602A (en) | All-protein detecting method | |
| Turner et al. | Ortho-phthalaldehyde as a spray reagent for thin layer Chromatograms | |
| JPH0257673B2 (en) | ||
| JPS6130216B2 (en) | ||
| JPH0623744B2 (en) | Test piece for nitrite detection | |
| Rajagopal et al. | A new method for estimation of ethylene glycol in biological material | |
| JPS623903B2 (en) | ||
| US4543336A (en) | Indicator for determining sulfur dioxide |