CN101000309A - Test paper for quickly investigating formol content in aquatic product - Google Patents

Test paper for quickly investigating formol content in aquatic product Download PDF

Info

Publication number
CN101000309A
CN101000309A CN 200610124302 CN200610124302A CN101000309A CN 101000309 A CN101000309 A CN 101000309A CN 200610124302 CN200610124302 CN 200610124302 CN 200610124302 A CN200610124302 A CN 200610124302A CN 101000309 A CN101000309 A CN 101000309A
Authority
CN
China
Prior art keywords
test paper
formaldehyde
milliliters
content
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN 200610124302
Other languages
Chinese (zh)
Inventor
鲁统部
李晶平
陆慧宁
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sun Yat Sen University
Original Assignee
Sun Yat Sen University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sun Yat Sen University filed Critical Sun Yat Sen University
Priority to CN 200610124302 priority Critical patent/CN101000309A/en
Publication of CN101000309A publication Critical patent/CN101000309A/en
Pending legal-status Critical Current

Links

Landscapes

  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The invention discloses aquatic product formaldehyde content fast detecting test paper. It improves color developing reagent formula that 10-50ml ethanol, 2ml acetylacetone, 10-25g ammonium acetate are adding water to make up to volume at 100ml. The test paper can be cut into needed strip, packaged by black packing material. While liquid drops one the test paper, or it is directly immerged into the liquid, the wetting part of the test paper will turn yellow in two minutes if the formaldehyde content is more than 20ppm. Then it is compared with the standard color contrasting card to judge formaldehyde content in the detecting liquid. The detecting test paper of the invention can improve detecting sensitivity and stability, shorten drying time, and increase production efficiency.

Description

Test paper for quickly investigating formol content in the aquatic products
Technical field
The present invention relates to the food safety detection field, specifically, relate to a kind of can the fast detecting aquatic products in the test paper of content of formaldehyde.
Background technology
Formaldehyde has another name called formaldehyde; English name is: Formaldehyde or Methanal; Chemical formula is CH 2O; Under the normal temperature a kind of colourless, gas with intense stimulus, fusing point-92 ℃, boiling point-19.5 ℃, relative density 0.815; Multiple organic solvents such as soluble in water and ethanol, concentration of aqueous solution reaches as high as 55%.Formaldehyde in rare formalin almost all changes into its hydrate methylene glycol CH 2(OH) 2Denseer formalin (as formalin) through the placement of long period or make it slowly to evaporate, then aggregate into paraformaldehyde (CH 2O) n, n=6~50.
Formaldehyde is poisonous, sucks that formaldehyde vapors can cause nausea, rhinitis, bronchitis and conjunctivitis etc.Formaldehyde directly contacts with skin and can cause and burn.In June, 2004, the report delivered of IARC of the World Health Organization (WHO) claimed that the formaldehyde of high concentration can cause ear, nose and laryngocarcinoma, also mentioned also simultaneously causing leukaemia.
Formaldehyde in food if enter human body through the oral cavity, can exert an influence to digestion, nerve, circulation and urinary system, and severe patient is with threat to life.Wrongly take formalin, can cause oral cavity, oesophagus, gastrointestinal damage, cause the alimentary canal perforation, or cause shock, stupor and liver, renal damage.Digestive system shows as: can occur feeling sick, vomitting.As be oral ingestion, can cause mouth, pharynx, oesophagus and stomach burn feeling, the erosion of companion's oral mucosa, epigastric pain rejects the band bloody vomit; Gastric-intestinal perforation and liver damage take place when serious.Because formaldehyde is converted into methyl alcohol in vivo, can produce anesthesia to nervous system, performance: headache, giddy, eye-blurred and weak constitutional symptom are arranged; Stupor can appear when serious.Circulation system performance: for heart rate is accelerated elevation of blood pressure; Blood pressure drops, shock can appear when serious, even bradycardia, ventricular fibrillation, cardiac arrest.The urinary system performance: serious oral ingestion can cause kidney damage performances such as albuminuria, renal shutdown, uremia.The per os lethal quantity of wrongly taking formalin is about 10~20ml.
Formaldehyde must not detect in most of food for the explicitly provided adjuvant that uses in food forbidden of country, and Ministry of Agriculture's regulation water-soaked food Background Value of Formaldehyde is limited the quantity of and is 10ppm.The aquatic products that use formalin to soak can be anticorrosion, and can brighten, change color and luster, sends out aquatic products various so some illegal retailer steeps with it, extending the shelf life, and increase toughness and crisp sense; Also some illegal retailer fumigates even it is directly joined in wheaten food, mushroom or the bean product with it, reaches the purpose that increases certainly, changes color and luster, thus serious threat people's health.
The method of measuring content of formaldehyde at present has vapor-phase chromatography, liquid phase chromatography, gas-matter coupling method, variable color acid system, acetylacetone method etc.Yet above detection method complex operation, strongly professional is not suitable for the ordinary people and uses.To use simple, result to judge clear and detect test paper, is fit to popular the use.
The at present known patent that detects test paper about formaldehyde in food has only two kinds: a kind of for " being used to detect test paper, test solution and the detection method thereof of formaldehyde, methanol content " (CN1256421), this test paper is to be suctioned after Schiff's reagent or class be Dick's reagent by common filter paper, dry forming under the cryogenic vacuum condition.Its shortcoming is in measuring aquatic products during content of formaldehyde, and the protein in the aquatic products seriously disturbs its chromogenic reaction, thereby has influence on the accuracy of testing result.Another for " be not subjected to protein disturb fast detecting water send out or aquatic products in the test paper of formaldehyde composition " (CN1487296), this test paper is that common test paper is cut into strip, soak into fully the aqueous solution reagent of forming by diacetone-ammonium acetate 2 minutes, take out back drying under room temperature lucifuge condition and form.Be slightly soluble in neutral water owing to diacetone is soluble in acidulous water, therefore its diacetone of reagent of allotting of the developer prescription that provides by this patent can not be fully and water miscible, cause the on the low side and skewness of diacetone content in water.Simultaneously,, easily resolve into acetic acid and acetone, cause this developer instability, need matching while using, thereby bring very big constant to practical application because diacetone is unstable in water.Its sensitivity that detects formaldehyde of test paper that goes out with this developer formulation only is 30ppm, can not detect the formaldehyde that contains in the following aquatic products of 30ppm, thereby can't guarantee the safety of aquatic products.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of highly sensitive, developer good stability, the test paper of content of formaldehyde in the convenient and practical fast detecting aquatic products.
For achieving the above object, the present invention improves the developer prescription.
Developer prescription of the present invention is to contain 10~50 milliliters of ethanol in per 100 ml solns, 2 milliliters of diacetones, and ammonium acetate 10~25 grams add water and are settled to 100 milliliters.Optimum formula is to contain 50 milliliters of ethanol in per 100 ml solns, 2 milliliters of diacetones, and ammonium acetate 25 grams add water and are settled to 100 milliliters.
The preparation method who detects test paper is: common filter paper is soaked taking-up after 2 minutes in developer, at room temperature vacuum drying forms then.Test paper can be cut into the strip of suitable size as required, and packs with the black wrappage, with lucifuge and prevent that oxidation by air from making the test paper flavescence.
Detection method is: when detecting the aquatic products content of formaldehyde, with drop to be measured to test paper, or directly immerse test paper in the liquid to be measured, when content of formaldehyde is greater than 20ppm in the liquid to be measured, flavescence in about 2 minutes of the test paper wetted portions contrasts the content that can judge formaldehyde in the liquid to be measured with the yellow color depth and the standard color comparison card that shows.
Compared with prior art, the present invention has following beneficial effect: the present invention is by improving the existing developer prescription that detects test paper:
(1) by in developer, adding absolute ethyl alcohol, improved the solubleness of diacetone in developer, the test paper sensitivity of making reaches 20ppm, surpasses the 30ppm of prior art.
(2) by in developer, adding absolute ethyl alcohol, improve the stability of diacetone, prolonged the stationary phase of developer.
(3) by in developer, adding absolute ethyl alcohol, shorten the drying time of test paper, improved production efficiency.
Embodiment
Embodiment 1:
Formaldehyde detection test paper with method for preparing detects a collection of formalin of randomly drawing out (concerning using formaldehyde test paper person from the formaldehyde titer of concentration known, the concentration of formaldehyde all is unknown in this batch solution), the user according to the result of chromogenic reaction judge have in the solution formaldehydeless, and and normal concentration contrast the correctness of judging testing result, judge that with this self-control formaldehyde detects the practicality of test paper.Testing result sees Table 1:
Table 1
1 2 3 4 5 6 7 8 9 10
The result - + + - + + - - - -
Concentration (ppm) 15 50 35 10 25 40 5 0 10 10
Annotate: "+" representative colour developing is the result be positive, and "-" representative colour developing is the result be negative
Embodiment 2:
Formaldehyde detection test paper with method for preparing detects a collection of formalin of randomly drawing out (concerning using formaldehyde test paper person from the formaldehyde titer of concentration known, the concentration of formaldehyde all is unknown in this batch solution), the user according to the result of chromogenic reaction judge have in the solution formaldehydeless, and and normal concentration contrast the correctness of judging testing result, judge that with this self-control formaldehyde detects the practicality of test paper.Testing result sees Table 2:
Table 2
1 2 3 4 5 6 7 8 9 10
The result - + + + - + + + + -
Concentration (ppm) 0 30 50 25 10 30 35 40 50 5
Annotate: "+" representative colour developing is the result be positive, and "-" representative colour developing is the result be negative
Embodiment 3:
Formaldehyde detection test paper with method for preparing detects a collection of formalin of randomly drawing out (concerning using formaldehyde test paper person from the formaldehyde titer of concentration known, the concentration of formaldehyde all is unknown in this batch solution), the user according to the result of chromogenic reaction judge have in the solution formaldehydeless, and and normal concentration contrast the correctness of judging testing result, judge that with this self-control formaldehyde detects the practicality of test paper.Testing result sees Table 3:
Table 3
1 2 3 4 5 6 7 8 9 10
The result - + + + - - - - + +
Concentration (ppm) 0 25 35 25 5 15 0 10 20 40
Annotate: "+" representative colour developing is the result be positive, and "-" representative colour developing is the result be negative
By above-mentioned three embodiment, detect 30 samples altogether, result and normal concentration contrast show accurately to detect the formaldehyde sample of concentration greater than 20ppm.

Claims (3)

1, the quick detection test paper of content of formaldehyde in a kind of aquatic products comprises test paper and the reagent for preparing test paper, it is characterized in that:
(1) reagent of preparation test paper is developer, and prescription is to contain 10~50 milliliters of ethanol in per 100 ml solns, 2 milliliters of diacetones, and ammonium acetate 10~25 grams add water and are settled to 100 milliliters;
(2) test paper is soaked in developer, vacuum drying at room temperature forms after the taking-up.
2, quick detection test paper as claimed in claim 1, the prescription that it is characterized in that described developer are to contain 50 milliliters of ethanol in per 100 ml solns, 2 milliliters of diacetones, and ammonium acetate 25 grams add water and are settled to 100 milliliters.
3, utilize the method for content of formaldehyde in claim 1 or the 2 described detection paper aquatic products, it is characterized in that: with drop to be measured to test paper, or directly immerse test paper in the liquid to be measured, when content of formaldehyde is greater than 20ppm in the liquid to be measured, flavescence in about 2 minutes of the test paper wetted portions contrasts the content that can judge formaldehyde in the liquid to be measured with the yellow color depth and the standard color comparison card that shows.
CN 200610124302 2006-12-19 2006-12-19 Test paper for quickly investigating formol content in aquatic product Pending CN101000309A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200610124302 CN101000309A (en) 2006-12-19 2006-12-19 Test paper for quickly investigating formol content in aquatic product

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200610124302 CN101000309A (en) 2006-12-19 2006-12-19 Test paper for quickly investigating formol content in aquatic product

Publications (1)

Publication Number Publication Date
CN101000309A true CN101000309A (en) 2007-07-18

Family

ID=38692367

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200610124302 Pending CN101000309A (en) 2006-12-19 2006-12-19 Test paper for quickly investigating formol content in aquatic product

Country Status (1)

Country Link
CN (1) CN101000309A (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102221546A (en) * 2010-11-25 2011-10-19 苏州工业园区安泽汶环保技术有限公司 Detection test paper for formaldehyde and/or acetaldehyde in air, detection method thereof and preparation method for detection test paper
CN102353673A (en) * 2011-09-30 2012-02-15 哈尔滨工业大学 Formaldehyde test paper and preparation method thereof
CN103196902A (en) * 2012-01-04 2013-07-10 窦荣艳 Air formaldehyde self-detecting box
CN103616380A (en) * 2013-12-03 2014-03-05 广东中测食品化妆品安全评价中心有限公司 Reagent and method for rapidly detecting formaldehyde in foods
CN104034682A (en) * 2014-06-27 2014-09-10 宜特科技(昆山)电子有限公司 Method for measuring formaldehyde emission amount of interior decoration material
CN104076034A (en) * 2014-07-17 2014-10-01 北京福德安科技有限公司 RGB quantitative quick detecting test paper for formaldehyde residue in food
CN104359902A (en) * 2014-11-13 2015-02-18 上海应用技术学院 Formaldehyde colour-rendering test paper, and preparation method and application thereof
CN107741420A (en) * 2017-10-16 2018-02-27 祁忆青 A kind of device and method for preparing test paper of Rapid Detection of Formaldehyde burst size
CN113777098A (en) * 2020-06-10 2021-12-10 四川精卫食品检测科技有限公司 Method for rapidly determining content of methanol in white spirit
CN113984753A (en) * 2021-11-04 2022-01-28 长春美泰仪器有限公司 Formaldehyde detection test paper, preparation method thereof and formaldehyde detection system
CN117969500A (en) * 2024-03-27 2024-05-03 山东新蓝环保科技有限公司 Method for detecting aldehydes in urea aqueous solution of nitrogen oxide reducing agent

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102221546A (en) * 2010-11-25 2011-10-19 苏州工业园区安泽汶环保技术有限公司 Detection test paper for formaldehyde and/or acetaldehyde in air, detection method thereof and preparation method for detection test paper
CN102221546B (en) * 2010-11-25 2012-11-07 苏州工业园区安泽汶环保技术有限公司 Detection test paper for formaldehyde and/or acetaldehyde in air, detection method thereof and preparation method for detection test paper
CN102353673A (en) * 2011-09-30 2012-02-15 哈尔滨工业大学 Formaldehyde test paper and preparation method thereof
CN103196902A (en) * 2012-01-04 2013-07-10 窦荣艳 Air formaldehyde self-detecting box
CN103616380A (en) * 2013-12-03 2014-03-05 广东中测食品化妆品安全评价中心有限公司 Reagent and method for rapidly detecting formaldehyde in foods
CN104034682A (en) * 2014-06-27 2014-09-10 宜特科技(昆山)电子有限公司 Method for measuring formaldehyde emission amount of interior decoration material
CN104076034A (en) * 2014-07-17 2014-10-01 北京福德安科技有限公司 RGB quantitative quick detecting test paper for formaldehyde residue in food
CN104359902A (en) * 2014-11-13 2015-02-18 上海应用技术学院 Formaldehyde colour-rendering test paper, and preparation method and application thereof
CN104359902B (en) * 2014-11-13 2017-05-03 上海应用技术学院 Formaldehyde colour-rendering test paper, and preparation method and application thereof
CN107741420A (en) * 2017-10-16 2018-02-27 祁忆青 A kind of device and method for preparing test paper of Rapid Detection of Formaldehyde burst size
CN113777098A (en) * 2020-06-10 2021-12-10 四川精卫食品检测科技有限公司 Method for rapidly determining content of methanol in white spirit
CN113984753A (en) * 2021-11-04 2022-01-28 长春美泰仪器有限公司 Formaldehyde detection test paper, preparation method thereof and formaldehyde detection system
CN117969500A (en) * 2024-03-27 2024-05-03 山东新蓝环保科技有限公司 Method for detecting aldehydes in urea aqueous solution of nitrogen oxide reducing agent
CN117969500B (en) * 2024-03-27 2024-06-07 山东新蓝环保科技有限公司 Method for detecting aldehydes in urea aqueous solution of nitrogen oxide reducing agent

Similar Documents

Publication Publication Date Title
CN101000309A (en) Test paper for quickly investigating formol content in aquatic product
JPS6370151A (en) Luminescent oxygen sensor based on lanthanide complex
CN102262088A (en) Quick methanol detection device and manufacturing method thereof
US4328182A (en) Sterilization indicators containing amino acid(s) and pH indicator
CN104198448B (en) Probe for detecting aluminum ion, preparation method and kit including the probe
CN102590193B (en) Method for measuring sulfur dioxide content in food
Ogunwale et al. A novel method of nicotine quantification in electronic cigarette liquids and aerosols
JP3377005B2 (en) How to diagnose intestinal inflammatory conditions
CN112858508B (en) Method for determining furfural compounds in coffee product
Nishizawa et al. Presence of peroxyradicals in cigarette smoke and the scavenging effect of shikonin, a naphthoquinone pigment
Zhang et al. Pycnogenol® in cigarette filters scavenges free radicals and reduces mutagenicity and toxicity of tobacco smoke in vivo
Goodspeed et al. Sensitive and specific gas-liquid chromatographic-spectrophotometric screening procedure for trace levels of five sulfonamides in liver, kidney, and muscle tissue
CN101700267B (en) Detection method of kaempferol and quercetin content of persimmon leaf
JP5796863B1 (en) Tumor examination composition and tumor examination test paper
US4325908A (en) Theophylline test
CN103926229A (en) Novel reagent and method for detecting formaldehyde in food
Richter et al. The rate of removal of amines from the blood
CN107162930B (en) Fluorescence probe of superoxide anion and its preparation method and application, fibre-optical probe and preparation method thereof for identification
CN1967217A (en) Easy and rapid method for detecting formaldehyde in environment and food
Stein et al. Determination of fluorocarbon in blood
Filer et al. Decoquinate: I.—An absorption and elimination study in broiler chickens using 14C‐labelled decoquinate
CN112680216B (en) Fluorescent probe for ammonia detection and preparation method and application of test paper thereof
JPH0380260B2 (en)
Meijer et al. Exhaled nitric oxide concentration is influenced by alcohol containing disinfectants
James The oxidation of sulphanilamide and sulphapyridine by hydrogen peroxide

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication