JPS6249257A - Inspection paper set for quick qualitative inspection of vanillyl mandelate in urine and use thereof - Google Patents

Abstract

PURPOSE:To enable quick and handy qualitative inspection of VMA (vanillyl mandelate), by using three kinds of inspection papers dried after impregnated respectively with a paranitroaniline chloric acid solution, a sodium nitrile aqueous solution and a potassium carbonate aqueous solution. CONSTITUTION:For example, filter papers are impregnated with a paranitroaniline chloric acid solution, a sodium nitrile aqueous solution and a potassium carbonate aqueous solution at the concentration of 1%, 2% and 15% respectively and dried to make a A inspection paper 2, a B inspection paper 3 and a C inspection paper 1. Then, the C inspection paper 1 is laminated with the A inspection paper 2 and the B inspection paper 3 and then, a small amount of urine 5 is dropped with a pipet 4 from above the B inspection paper 3. The urine 5 dropped makes a reaction while passing through the B inspection paper 3 and the A inspection paper 2 and exudes to the C inspection paper 1. VMA presents purple with a color reaction to check for the presence of VMA in the urine. This enables quick and accurate qualitative inspection of VMA.

Description

Detailed Description of the Invention (Industrial Field of Application) The present invention relates to a qualitative test strip set and method for urinary vanillylmandelic acid (hereinafter abbreviated as VMA).
A test strip set that quickly and easily performs a qualitative test of VMA using three types of dry test strips that have been mixed with paranitroaniline hydrochloric acid solution, sodium nitrite aqueous solution, and potassium carbonate aqueous solution. Regarding inspection methods.

(Prior art) Conventionally, as a rapid test method for VIA in urine, a patient's urine is taken into a pipette, a drop of the urine is dropped onto a piece of paper, and the weight is smeared onto a piece of paper so that it is about 5 to 10 fins in diameter. After drying, a chemical solution consisting of a 1:1:2 mixture of 0.1% paranitroaniline hydrochloric acid solution, 0.2% sodium nitrite aqueous solution, and 10% potassium carbonate aqueous solution (mixed How to spray (7) and see the VIA turn purple (
(hereinafter referred to as the spot method), or a method in which the patient's urine 1517 is placed in a small test tube, the same amount of the mixture is added to it, and the coloration is observed as a wine-red color against a white paper background (hereinafter referred to as the test tube method). )
The inspection is being conducted by.

(Problems to be Solved by the Invention) 1) However, if the mixed solution used in the spot method or test tube method is not used immediately after preparation, the potency will decrease or disappear. The liquid is kept separately at 2°C to 10°C.
When storing the product under refrigeration and carrying out the test, a mixed solution had to be prepared, which was a very complicated operation.

(Means for Solving the Problem) The present invention provides a rapid qualitative test strip for VMA in urine that can eliminate and improve the drawbacks and problems of conventional methods and means, and a test using the test strip. The object of the present invention is to provide a method for reducing V in urine as described in the claims.
The above object can be achieved by providing a rapid qualitative test strip set for MA and a test method using the test strip set. That is, this invention provides an A test paper that is impregnated with a paranitroaniline hydrochloric acid solution and then dries and peels;
1. Nitrous acid) IJum aqueous solution. Let it soak in and dry.
There are three types of test paper: B test paper, which lasts a long time, and C test paper, which is soaked with an aqueous potassium carbonate solution, weighed down, and dried. Place A test paper with an area smaller than the area of the mount on top of it (7. Place A test paper on the A test paper).
After weighing a test strip B, which has approximately the same area as the test strip, the urine dropped onto the test strip B permeates through the test strip A and then into the test strip C, resulting in a color reaction that causes vanillyl mandel in the urine to be detected. This article relates to a test strip set for rapid qualitative testing of acids and its usage.

Examples of the test strip and test method of the present invention will be described in detail below.

The test paper of the present invention is a piece of paper, for example, that has been impregnated with the following three types of chemicals and dried.

That is, one paper was mixed with paranitroaniline 1y + concentrated hydrochloric acid I
Qm/, add distilled water to bring the total volume to 100 ml,
Immerse it in a 1% paranitroaniline hydrochloric acid solution, drain off the excess chemical solution, and quickly dry it at an appropriate temperature. One piece of dried paper is cut into discs with a diameter of about 8 squares and used as A test paper.

Next, add a new piece of paper to 21% of sodium nitrite. distilled water 981
Immerse it in a 2% solution of sulfur and lithium nitrite, drain off the excess chemical solution, and quickly dry it at an appropriate temperature. 1. Cut the dried paper into disks about 8 mm in diameter. and use it as the B test strip.

Next, add a new piece of paper to carbonation power 'J 15 S' + distilled water 8
It is soaked in a 15% potassium carbonate aqueous solution consisting of 5IIt, the excess chemical solution is removed, and it is rapidly dried by drying at an appropriate temperature.

The dried paper is cut into a size that is easy to test and used as 1°C test paper.

To dry the test strips at the appropriate temperature, select the optimal temperature for each test strip.
The process is carried out rapidly at a temperature between 30 and 150°C for a certain period of time in a gas flow such as air or nitrogen. If necessary, drying may be carried out by vacuum drying at low temperatures. In the above example, the concentration of the paranitroaniline hydrochloric acid solution is 1%, the sodium nitrite solution is 2%, and the potassium carbonate solution is 15%. All you have to do is decide the concentration.

Next, the inspection method will be explained.

FIG. 1 shows the procedure of the inspection method of the present invention. Place the A test paper 2 on top of the C test paper 1, then place the B test paper 3 on top of that. Drip a small amount of urine 5 onto the B test paper 3 using a pipette 4. 5 drops of urine B test paper 3. While passing through the A test strip 2, it reacts and oozes out onto the C test strip 1 [7], producing a purple color reaction of VIA, confirming the presence of VIA in the urine. If necessary, place a transparent glass plate or resin plate with a hole in the center on top of the C test paper, and insert the A test paper and B into the hole.
If a test paper is inserted, there will be no misalignment between A and B, and an appropriate spacing will be maintained, which is convenient when testing a large amount.

In addition, reactions can be clearly seen at a glance, and each person's name can be written on the transparent plate on the top, so confusion with others can be prevented.

In the method of the present invention, each drug used is soaked and weighed on a separate P paper, so the amount of drug used can be adjusted by the size of one paper, and therefore the size of one paper is determined by the amount of urine used. Furthermore, the ratio of chemicals to be used can be determined by the sizes of the A and B test strips.

In the test method of the present invention, an A- test strip is placed on a G test strip, and a B test strip is placed on top of the G test strip, and an appropriate amount of urine is dripped on top of the B test strip. Through the paper 1. As it oozes out, a chemical reaction occurs sequentially with an appropriate amount of the drug.] 2) Qualitative testing of VMA can be performed quickly and accurately.

4...pipette, 5...urine.

(Effects of the present invention) In the conventional testing method, test chemical solutions had to be stored separately in a refrigerator and a mixed solution had to be prepared each time a test was performed, but in the method of the present invention, Since the drug is soaked onto a piece of paper and dried, it can be stored for a long time without losing its effectiveness by storing it in the refrigerator.When using it, all you have to do is take out the fP paper and stack it, so you can mix it each time you use it. The operation of preparing medicine can be omitted.

Furthermore, since the amount of drug used can be reduced to the size of one paper, the ratio of drug to urine volume can be set appropriately, so that the chemical reaction is sufficiently completed while the urine oozes through the test paper. The operation is carried out simply by folding 71 pieces of paper and dropping urine from -F Hoyone Shosha, which makes it possible to conduct a qualitative test of VMA very quickly and accurately, which is extremely effective for medical treatment and testing.

[Brief explanation of drawings]

FIG. 1 is an explanatory diagram showing the procedure of the inspection method of the present invention.

Claims (1)

[Claims] 1. A test paper impregnated with paranitroaniline hydrochloric acid solution and then dried; B test paper impregnated with an aqueous sodium nitrite solution and then dried; A test paper impregnated with an aqueous potassium carbonate solution. The A test paper is made of three types of test paper: a C test paper that is dried after drying; After placing test paper B, which has approximately the same area as test paper A, on top of the paper, the urine dropped onto test paper B permeates through test paper A and test paper C, resulting in a color reaction that causes the color reaction in the urine to occur. Test paper set for rapid qualitative testing of vanillylmandelic acid. 2. In the rapid qualitative test method for vanillylmandelic acid in urine: A test paper impregnated with paranitroaniline hydrochloric acid solution and dried; B impregnated with an aqueous sodium nitrite solution and dried.
Test paper; Test paper C soaked with an aqueous solution of potassium carbonate and dried; Test paper A and Test paper B made into small sections of approximately equal area, and a large area A test paper A is placed on the C test paper, and then a B test paper is placed on the A test paper; a trace amount of urine is dropped onto the B test paper to cause a color reaction on the C test paper. Rapid qualitative test method for vanillylmandelic acid.