JPS6243140B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an improvement in a method for aspirating a test liquid in a blood test performed using a titration plate made of synthetic resin in a university or hospital laboratory.

For example, in the case of blood enzyme immunoassay, a titration plate made of synthetic resin has a large number of test holes arranged vertically and horizontally. Drop antibody-attached beads with antibodies attached to the surface of glass beads into each hole of the plate, and
After injecting the sample into the sample and allowing it to react for a certain period of time, the titration plate is set in a test device that can dispense the test solution using a pump device and remove the dispensed test solution by suction. , one cycle of dispensing a test solution (washing buffer) to each hole and then suctioning out the dispensed test solution is repeated 5 to 6 times.
Repeat several times to wash the inner wall surface of each hole of the titration plate and the surface of the antibody-attached beads, and then
The enzyme activity on the surface of the beads is measured by irradiating the beads with fluorescent light using a fluorophotometer.

When testing blood, etc. using a titration plate made of synthetic resin, the titration plate is made of synthetic resin, so it becomes charged with static electricity. There is a problem that the hole adheres to the inner wall surface and is difficult to peel off, and remains on the inner wall surface of the hole after dispensing the test liquid and cleaning by suction, making the test value inaccurate.

The present invention was made to solve this problem, and after repeated various studies, it was found that the static electricity that builds up on the titration plate during testing can be removed by suctioning the test liquid dispensed into each hole. This occurs when the sample liquid being aspirated and the air flow violently come into contact with the inner wall of the hole during evacuation, especially when the hole is empty at the end of the aspiration process. This is caused by contact with the air flow and the flow of fine particles of the test liquid under the conditions, and the suction of the test liquid must be stopped to the extent that only a small amount of the test liquid remains in the hole. By doing so,
This technology was completed based on the knowledge that the amount of static electricity generated (charged amount) can be significantly reduced.

For this reason, in the present invention, a titration plate made of synthetic resin is set in an inspection device capable of dispensing a test solution and aspirating the dispensed test solution, and a titration plate made of synthetic resin is formed on the titration plate. In a method for testing blood, etc., in which one cycle of dispensing a test liquid using the testing device and suctioning the dispensed test liquid into the hall is repeated several times, the suction of the test liquid during the testing process is as follows: The characteristic is that the suction stroke is set to such an extent that a small amount of test liquid remains at the bottom of the hole, and only the suction of the test liquid in the final step is performed with a suction stroke set to aspirate all of the test liquid in the hole. This paper proposes a method for aspirating a test liquid in a blood test using a titration plate made of synthetic resin.

Next, embodiments will be described in detail with reference to the drawings.

FIG. 3 is a longitudinal cross-sectional view of a titration plate 1 made of synthetic resin, in which numerals 10 indicate a large number of holes aligned in the horizontal direction, and these holes 10 are further aligned in the vertical direction. It has been done.

FIG. 1 is a front view showing an inspection apparatus 2 that performs various inspections using the titration plate 1.

The test device 2 discharges a specified amount of test liquid through the discharge section 3 into each hole 10 of the titration plate 1 which is movably supported by an actuating device (FIG. 4), and the discharged test liquid is It is sufficient that the suction part 5 is configured to suction the liquid, and any suitable means may be used for the suction part 5, the discharge part 3, and the actuating device. Therefore, in the discharge section 3 of this embodiment, as shown in FIG.
A pump support frame 30 mounted and supported by a support frame 22 provided in FIG. The pistons 33 are fitted into each cylinder 32 so as to be able to move up and down freely, and the heads of the pistons 33 are inserted into the respective cylinders 32 so as to correspond to the holes 10 provided in the titration plate 1. The pump device 36 is constructed by being supported by a plate plate 35 that is supported at four positions on the upper surface side of the pump support frame 30 so as to be movable up and down and biased upward by springs 34. By pressing down the plate 35 against the spring 34 by the pressing device 4, each piston 33 plunges into each cylinder 32 and performs a dispensing (dispensing) operation, and by releasing the pressure, the spring 34 Each piston 33 is raised by the operation of the plate 35 which returns and rises, thereby performing a suction operation. The cylinder 3 is designed so that the amount that can be drawn up and dispensed from the dispensing needle 31 at one time is 0.1 ml at the minimum and 3 ml at the maximum.
2 and the pushing stroke of the piston 33 are set respectively.

Further, the pressing device 4 includes a piston pressing plate 4 assembled on the upper surface side of the plate plate 35, as shown in FIG.
At the four corners of the upper surface of 0, threaded cylinders 41 are respectively installed to provide four-point support, and threaded rods 42 are attached to them.
are screwed together, and the upper end portions of the threaded rods 42 are rotatably supported by the support frame 22, and are made to protrude above the support frame 22 on the upper end side, and are respectively attached to the protruding portions. A conduction chain 45 is wound around the sprocket 43 and the sprocket 44 provided on the rotating shaft of the piston push plate driving motor M installed on the side of the support frame 22. The piston push plate 40 is moved up and down by rotating each threaded rod 42 clockwise and counterclockwise through forward and reverse rotation. The piston push plate driving motor M is made for low-speed rotation, and has a rotating shaft provided with the sprocket 44 extending downward, and a disk 47 is attached to the extending shaft 46, and the circular plate 47 is attached to the extending shaft 46. A large number of notch holes 48 that open in the radial direction are provided on the outer periphery of the plate 47 in parallel in the circumferential direction, and switches S and S (phototubes) corresponding to the upper and lower positions are arranged in the upper and lower positions of the notch holes 48,
The operation of the piston push plate driving motor M is controlled by the switches S and S by counting the number of notched holes 48 formed by the rotation of the disk 47, as shown in FIG. Front panel 2 of aircraft a
When the knob 25 is set to the dispensing amount displayed on the digital control board 24 provided at the piston 3, for example 2 milliliters, the piston 3
A piston push plate driving motor M operates to move the piston plate 3 up and down.

Reference numeral 5 denotes a suction section arranged in parallel with the discharge section 3 as shown in FIG.

The suction unit 5 has 50 downward suction needles 51 formed in the same manner as injection needles arranged vertically and horizontally and supported on a bottom plate member 50 of a sealed chamber 52 arranged above the machine stand 21. The bottom plate member 5 of the sealed chamber 52
0 and the suction port 6 of the suction pump 6
1 through a pipe 62, and the suction pump 6 operates to suction from each suction needle 51 at once, and the suction stroke is as follows:
In this embodiment, control is performed using a timer device 7 and a control valve 70 that opens and closes the passage (pipe 62) for a certain period of time according to the timer device 7.

The timer device 7 is a conventionally known device that operates for a set time in response to an activation signal, but can now be set in two stages so that it can be activated in response to an activation signal during the inspection process and a signal at the end of the inspection process. ing. For example, dispensing a test solution and removing it by suction.
When repeating the cycle 5 times, the first stage is from the first cycle to the fourth cycle, and the activation time for each cycle is set to 12 seconds, and the second stage is from the last cycle. If you set it to 6 seconds per stroke, it will operate for 3 seconds each up to 4 times, and by controlling the operation of the control valve 70 so that the passage is open for 6 seconds only in the final stroke, the suction stroke (suction time) will be reduced only in the final stroke. There is a way to make it longer.

Reference numeral 8 denotes a set table which is reciprocated by the actuating device so as to face the lower part of the suction part 5 and the discharge part 3, and which can move forward and backward with respect to the dispensing needles 31 and the suction needles 51; 1 is set.

The set table 8 is constructed by installing a vertical rod 83 that can be raised and lowered on a cart 82 that slides along rails 81 and 81 that span the left and right sides of the machine body a, as shown in FIGS. 4 and 5. Support motor M1,
It is controlled by M2 to freely move up and down and reciprocate left and right. That is, one of the motors M1 and M2, M1, is installed on the machine body a, and a transmission belt 85 is connected to a drive pulley m provided on its output shaft and to rolling wheels 84, 84 arranged on the left and right sides. The carriage 82 is connected to the transmission belt 85, and the drive pulley m is rotated in the normal and reverse directions by the forward and reverse rotation of the motor M1.
The other motor M2 operates to reciprocate left and right (arrow A) the underframe 82 of the truck 82.
A gear 86 installed on the rotating shaft of the motor M2 is engaged with the rack 8a, which is supported by a vertical guide 87 and integrally connected to the set table 8. The rotation of gear 8
6 is rotated in the forward and reverse directions, the setting table 8 is moved up and down (in the direction of arrow B). and,
The positioning of each stroke end in the vertical movement of the set table 8 and the reciprocating movement of the cart 82 is performed on each rail 8.
Counter switch S arranged on the left and right of 1,81
1. Counter switches S3 and S4 arranged above and below S2 and the set table 8 are connected to the trolley 82 and the set table 8.
Each motor M1, M2 is turned on and off by the signals from these switches, which are activated when the switches come in contact with each other.
This is done by controlling it off. At this time, the counter switch S4 located above the setting table 8 simultaneously sends an activation signal to the timer device 7 of the control valve 70. Note that these counter switches S1, S2, S3, and S4 are
When a desired value is set by operating the counter dial 26 provided on the front, the set value decreases each time it is operated, and it is a normal counter switch that stops when the value reaches zero. Reference numeral 9 denotes a pedestal on which a liquid dish 90 containing a test liquid (washing buffer) is placed, as shown in FIG. 4, and is movable up and down by another actuator. The actuating device engages a gear 91 provided on the rotating shaft of the motor M3 assembled in the machine body a with a rack 93 installed on the pedestal 9 supported by a vertical guide 92, thereby operating the motor. The gear 91 is rotated forward and backward by the forward and reverse operation of M2, thereby operating to move the pedestal 9 up and down. The positioning of each stroke end of this vertical movement is achieved by turning the motor M3 on and off by the signals sent when the pedestal 9 comes into contact with counter switches S5 and S6 arranged above and below, which actuate the switches S5 and S6. This is done by controlling it off. In addition, the counter switches S1, S
2, S3, S4, S5, S6 are shown in Figure 6 A, B,
As shown in C, D, E, and F, the sample solution sucked up from the liquid dish 9 in the starting amount is dispensed into each hole 10 of the titration plate 1, and the sample solution is sucked by the suction needle 51... Each motor M1, M
2. Switch and control M3.

Then, the titration plate 1 configured as described above is mounted and set on the setting table 8 of the inspection device 2, and the dispensing and suction of the test solution are repeated for each hole 10. When measuring enzyme activity after washing the antibody-attached beads,
When repeating the operations B, C, D, E, and F five times, for example, the suction time (suction stroke) in FIG.
Leave a small amount of the test solution at the bottom of the tube, and increase the suction time in the final step to aspirate it all.

As explained above, in the method of the present invention, a titration plate made of synthetic resin is set in an inspection device capable of dispensing a test solution and aspirating the dispensed test solution, and a titration plate made of synthetic resin is formed on the titration plate. For inside the hall,
In a method for testing blood, etc., in which one cycle of dispensing a test liquid using the test device and aspirating the dispensed test liquid is repeated several times, the test liquid is aspirated during the test process, and a small amount is deposited at the bottom of the hole. The suction stroke is set to such an extent that only 100% of the test liquid remains, and only the suction of the test liquid in the final step is performed with the suction stroke set to aspirate all of the test liquid in the hole. The static electricity that is generated and charged on the titration plate when the test solution is sucked out of the hole in the titration plate is suppressed significantly, and the cells in the sample are attracted to the inner wall of the hole due to the charged static electricity. , it is possible to effectively solve the problem of inaccurate test values when measuring by irradiating light.

[Brief explanation of the drawing]

Fig. 1 is a front view of the inspection device used for implementing the method of the present invention, Fig. 2 is a partially cutaway front view of the same discharge section,
Fig. 3 is a cross-sectional view with a part of the titration plate made of synthetic resin used in the above device omitted, Fig. 4 is an overall schematic explanatory diagram of the same device, and Fig. 5 is a side view of the setting table of the above device. Figure 6 A, B, C, D, H, and F are action explanatory diagrams. Explanation of drawing symbols, M...motor, S...switch, a...body, m...drive pulley, 1...titration plate, 10...hole, 2...inspection device, 2
1... Machine stand, 22... Support machine frame, 23... Panel, 24... Control board, 25... Knob, 26...
Counter dial, 3...Discharge part, 30...Pump device, 31...Dispensing needle, 32...Cylinder, 33...Piston, 34...Spring, 35...
Plate plate, 4... Pressing device, 40... Piston pushing plate, 41... Threaded tube, 42... Threaded rod, 43,
44...Sprocket, 45...Conduction chain, 4
6... Extension shaft, 47... Disc, 48... Notch hole,
5... Suction part, 50... Bottom plate member, 51... Suction needle, 52... Sealed chamber, 53... Connection port, 6... Pump device, 61... Suction port, 62... Pipe, 7
...Timer device, 70...Control valve, 8...Set stand, 8a...Rack, 81...Rail, 82...
...Dolly, 82a... Underframe, 83... Rod, 9...
...cradle, 90...liquid plate, 91...gear, 92...
Guide, 93...Rack,... Actuation device.

Claims (1)

[Claims] 1. Set a titration plate made of synthetic resin in a test device that can dispense a test solution and aspirate the dispensed test solution, and perform the test using the test device into the hole formed in the titration plate. In a method for testing blood, etc., in which one cycle of dispensing a liquid and aspirating the dispensed test liquid is repeated several times, a small amount of test liquid remains at the bottom of the hole when the test liquid is aspirated during the test process. Perform with a suction stroke set to a certain degree,
Aspiration of test liquid in tests for blood, etc. using a titration plate made of synthetic resin, characterized in that only the aspiration of the test liquid in the final step is performed with a suction stroke set to aspirate all of the test liquid in the hole. Method.