JPS62277548A - Ethanol sensor - Google Patents
Ethanol sensorInfo
- Publication number
- JPS62277548A JPS62277548A JP61121833A JP12183386A JPS62277548A JP S62277548 A JPS62277548 A JP S62277548A JP 61121833 A JP61121833 A JP 61121833A JP 12183386 A JP12183386 A JP 12183386A JP S62277548 A JPS62277548 A JP S62277548A
- Authority
- JP
- Japan
- Prior art keywords
- ethanol
- electrode
- concn
- value
- vessel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 109
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 210000000170 cell membrane Anatomy 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 abstract description 12
- 239000007789 gas Substances 0.000 abstract description 9
- 238000011088 calibration curve Methods 0.000 abstract description 8
- 230000007423 decrease Effects 0.000 abstract description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 5
- 239000001301 oxygen Substances 0.000 abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 229920005597 polymer membrane Polymers 0.000 description 6
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 5
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 4
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000005515 coenzyme Substances 0.000 description 3
- 229950006238 nadide Drugs 0.000 description 3
- 229920001220 nitrocellulos Polymers 0.000 description 3
- 108010025188 Alcohol oxidase Proteins 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005199 ultracentrifugation Methods 0.000 description 2
- 241000589236 Gluconobacter Species 0.000 description 1
- 241000185994 Pseudarthrobacter oxydans Species 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 229940101270 nicotinamide adenine dinucleotide (nad) Drugs 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
３、発明の詳細な説明
［産業上の利用分野］
本発明は溶液中のエタノールを選択的に感知してその濃
度を測定することができるエタノールセンサーに関する
。Detailed Description of the Invention 3. Detailed Description of the Invention [Field of Industrial Application] The present invention relates to an ethanol sensor that can selectively sense ethanol in a solution and measure its concentration.
［従来の技術］
溶液中のエタノールを感知するセンサーとしては、古く
は溶液中に浸したガス透過性チューブにキャリヤーガス
を流し、チューブ内に透過したエタノールを外部の検出
器（ガスクロマトグラフィー、赤外線ガス検出器など）
で検知する装置が知られている。このものはオンライン
計測が可能であり、実際の発酵液に適用してエタノール
測定に使用された例もあるが、装置が複雑で大型である
ため、エタノール測定を簡便に行えない不都合があった
。[Conventional technology] In the past, a sensor for sensing ethanol in a solution used a carrier gas flowed through a gas-permeable tube immersed in the solution, and the ethanol that permeated into the tube was detected using an external detector (gas chromatography, infrared rays, etc.). gas detector, etc.)
Devices that detect this are known. This device allows online measurement and has been used to measure ethanol in actual fermentation liquids, but the device is complex and large, making it difficult to measure ethanol easily.
これに対して、比較的小型のアルコールセンサーとして
は、酵素を用いたものが知られており、具体的には酵素
としてアルコールセンサ−ゼ（ＡＯＤ）を使用したもの
と、アルコールデヒドロゲナーゼ（ＡＤＩ（）を補酵素
であるニコチンアミドアデニンジヌクレオチド（ＮＡＤ
）と共に使用したものが開発されている。On the other hand, relatively small alcohol sensors that use enzymes are known, specifically those that use alcohol sensorase (AOD) and alcohol dehydrogenase (ADI). nicotinamide adenine dinucleotide (NAD), which is a coenzyme.
) has been developed for use with
（ＡＯＤ）
Ｒ−ＣＨ２０Ｈ＋ Ｏ，□
Ｒ−ＣＨ○ ＋ Ｈ２Ｏ２・・（１）
（ＡＤｌｌ）
Ｒ−ＣＨ２０Ｈ＋ ＮＡＤ”：
Ｒ−ＣＨ○ ＋Ｎ Ａ Ｄ Ｈ＋ Ｈ’ ・・（２）
前者は式（１）によって生成したＨ２Ｏ２又は消費さ九
た０２を、Ｈ２Ｏ，電極又は０２電極で測定するセンサ
ーであって、構成が簡単で比較的容易に製作できること
から、ビール、ワイン等のエタノール分析に利用された
実例がある。(AOD) R-CH20H+ O, □ R-CH○ + H2O2...(1) (ADll) R-CH20H+ NAD": R-CH○ +N A D H+ H'...(2)
The former is a sensor that measures H2O2 generated by formula (1) or consumed 02 with a H2O electrode or 02 electrode, and because it has a simple configuration and can be manufactured relatively easily, it is suitable for use with ethanol such as beer and wine. There are examples that have been used for analysis.
一方、後者のＡＤＨを用いるセンサーは、上式（２）に
よって生成したＮＡＤＨを電極でＮＡＤに酸化し、この
時流れる電流を測定するものである。On the other hand, the latter sensor using ADH oxidizes NADH generated by the above formula (2) to NAD using an electrode, and measures the current flowing at this time.
また、アルコール資化性酵母（Ｔｒｉｃｈｏｓｐｏｒｏ
ｎｂａｓｓｉｃａｅ）を０２電極上に固定し、酵母が資
化して呼吸活性が上昇することによる酸素の減少を。In addition, alcohol-assimilating yeast (Trichosporo
nbassicae) was immobilized on the 02 electrode, and the decrease in oxygen was caused by yeast assimilation and increased respiratory activity.
０２電極で測定するアルコールセンサーも従来知られて
いる。Alcohol sensors that measure using 02 electrodes are also conventionally known.
［発明が解決しようとする問題点］
従来知られている小型のアルコールセンサーのなかにあ
って、アルコールセンサ−ゼを使用したものは、現在使
用可能なアルコールオキシターゼ自体の選択性が低く、
メタノールにもエタノールと同様に感応するため、メタ
ノールが共存するエタノール溶液では、そのエタノール
濃度を正確に測定できない。一方、アルコールデヒドロ
ゲナーゼを使用するものは、選択性が高く、メタノール
には応答しない利点を備えているものの、電極の構成が
複雑であり、しかも高価なニコチンアミドアデニンジヌ
クレオチドを、補酵素として併用しなければならない不
利がある。[Problems to be Solved by the Invention] Among the conventionally known small-sized alcohol sensors, those using alcohol sensorase have low selectivity compared to currently available alcohol oxidase itself.
Since it is sensitive to methanol in the same way as ethanol, it is not possible to accurately measure the ethanol concentration in an ethanol solution in which methanol coexists. On the other hand, those that use alcohol dehydrogenase have the advantage of having high selectivity and not responding to methanol, but they have a complicated electrode structure and use expensive nicotinamide adenine dinucleotide as a coenzyme. There are disadvantages that must be met.
また、アルコール資化性酵母を使用したセンサーは、構
成が簡単で比較的容易に製作できる利点を有しているが
、低ｐＨでは酢酸等の有機酸にも、応答してしまう不都
合がある。Further, a sensor using alcohol-assimilating yeast has the advantage of having a simple structure and being relatively easy to produce, but has the disadvantage that it responds to organic acids such as acetic acid at low pH.
［問題、慨を解決するための手段］
本発明者等は、補酵゛素を必要としないアルコールデヒ
ドロゲナーゼと電子伝達系酵素とが共役した複合酵素群
（アルコール酸化酵素系）が。[Means for Solving the Problems and Problems] The present inventors have developed a complex enzyme group (alcohol oxidase system) in which alcohol dehydrogenase and electron transport enzyme are conjugated, which does not require co-fermenting elements.
酢酸菌の細胞膜に存在していることに着目し。We focused on its presence in the cell membrane of acetic acid bacteria.
この細胞膜を利用した新しいエタノールセンサーを開発
した。We developed a new ethanol sensor using this cell membrane.
すなわち、本発明に係るエタノールセンサーは、ｏ２電
極上に酢酸菌の細胞膜画分を固定化したことを特徴とし
、必要に応じて、前記細胞膜画分を固定化した。２電極
は、ガス透過性膜で覆うことができる。That is, the ethanol sensor according to the present invention is characterized in that the cell membrane fraction of acetic acid bacteria is immobilized on the O2 electrode, and the cell membrane fraction is immobilized as necessary. The two electrodes can be covered with a gas permeable membrane.
本発明で使用する酢酸菌の細胞膜画分は、酢酸菌として
一般的なアセトバクター（Ａｃｅｔｏｂａｃｔｓｒ）属
、グルコノバクタ−（Ｇｌｕｃｏｎｏｂａｃｔｅｒ）属
の菌株を１例えばフレンチプレス法、乳鉢による磨砕法
などの慣用の菌体破壊法で破砕し、この破砕物から超遠
心分離、限外濾過などの手段で得ることができる。また
、こうして取得された細胞膜画分を、ｏ２電極に固定化
する手段としては、ニトロセルロース等の多孔質高分子
膜に吸着させる吸着固定化法が採用できる外、天然又は
合成の高分子化合物内に、細胞膜画分を包括固定化する
包括固定化法も採用可能である。The cell membrane fraction of acetic acid bacteria used in the present invention can be prepared using a conventional method such as a French press method or a grinding method using a mortar. It can be crushed by the body destruction method and obtained from this crushed product by means such as ultracentrifugation and ultrafiltration. In addition, as a means of immobilizing the cell membrane fraction obtained in this way on the O2 electrode, adsorption and immobilization methods in which it is adsorbed on a porous polymer membrane such as nitrocellulose can be adopted, and in addition, in addition to the adsorption and immobilization method in which it is adsorbed on a porous polymer membrane such as nitrocellulose, Additionally, a comprehensive immobilization method in which cell membrane fractions are comprehensively immobilized can also be employed.
ガス透過性膜としては、多孔質テフロン膜等の疎水性多
孔質高分子膜が使用できる。As the gas permeable membrane, a hydrophobic porous polymer membrane such as a porous Teflon membrane can be used.
［作 用コ
本発明のエタノールセンサーをエタノール含有液に浸漬
すると、液中のエタノールは上記した細胞膜画分の固定
化膜内に浸透し１次式の通りアルデヒドに酸化されて０
２を消費する。[Function] When the ethanol sensor of the present invention is immersed in an ethanol-containing solution, the ethanol in the solution permeates into the immobilized membrane of the cell membrane fraction described above and is oxidized to aldehyde according to the linear equation.
Consumes 2.
（ＡＤＨ）
Ｒ−ＣＨ，○Ｈ−Ｒ−ＣＨ○＋２Ｈ”＋２ ｅ−＝（３
）全体としての反応は次のように示される。(ADH) R-CH,○H-R-CH○+2H"+2 e-=(3
) The overall reaction is shown as:
従って、この時の酸素消費量を０２電極で測定すること
により、液中のエタノール濃度を計測することができる
。Therefore, by measuring the amount of oxygen consumed at this time using the 02 electrode, the ethanol concentration in the liquid can be measured.
電極がガス透過性膜で覆われている場合には、エタノー
ルはこのガス透過性膜内を拡散透過して細胞膜画分の固
定化膜に到達するが、ガス透過性膜を付設することによ
り、エタノールセンサーは、広いｐＨ域で使用できるよ
うになり。When the electrode is covered with a gas-permeable membrane, ethanol diffuses through the gas-permeable membrane and reaches the cell membrane fraction immobilization membrane, but by attaching the gas-permeable membrane, Ethanol sensors can now be used over a wide pH range.
また、測定試料液中に混在する夾雑物が、電極及び固定
化膜に及ぼす悪影響を排除することができる。Further, it is possible to eliminate the adverse effects of contaminants mixed in the measurement sample liquid on the electrode and the immobilized membrane.
進んで、エタノール濃度の測定操作の一例を説明する。Next, an example of an operation for measuring ethanol concentration will be explained.
第１図に示す如く、０２電極１に酢酸菌細胞膜画分の固
定化膜２を装着し、さらにこれをガス透過性膜３で囲繞
して０リング７で固定した本発明のエタノールセンサー
を、記録計４に接続する。次いで、例えばウオタージャ
ケント付きビーカーのような液温を一定温度に保持する
ことができる容器５に０２ｆｆｉ極１を挿入し、容器内
を水又はＢ新液で満たしてマグネティックスクーラー６
等で攪拌する。容器内の液の溶存酸素が一定状態になる
と、０２電極の出力も安定する。この時の０２電極の出
力電流値を測定する。次に、濃度既知のエタノール溶液
を容器に添加すると、出力電流が減少する。As shown in FIG. 1, the ethanol sensor of the present invention has an 02 electrode 1 equipped with an immobilized membrane 2 of an acetic acid bacterium cell membrane fraction, which is further surrounded by a gas permeable membrane 3 and fixed with an 0 ring 7. Connect to recorder 4. Next, the 02ffi pole 1 is inserted into a container 5 that can maintain the liquid temperature at a constant temperature, such as a beaker with a water jacket, the container is filled with water or new liquid B, and the magnetic cooler 6 is inserted.
Stir with etc. When the dissolved oxygen in the liquid in the container becomes constant, the output of the 02 electrode also becomes stable. At this time, the output current value of the 02 electrode is measured. Next, when an ethanol solution of known concentration is added to the container, the output current decreases.
再び出力電流が一定になった時の電流値を測定して電流
減少値を計測する。種々の濃度のエタノール溶液を用い
て上と同様な操作を繰り返し、エタノール濃度と電流減
少値との関係を示す検量線をまず作成する。そのうえで
、濃度未知のエタノール含有試料液を用いて上と同様な
操作を行い、この時の電流減少値を求め、その値に対応
するエタノール濃度を先に作成した検量線で読み取るこ
とにより、試料液のエタノール濃度を知ることができる
。Measure the current value when the output current becomes constant again and measure the current reduction value. The same operation as above is repeated using ethanol solutions of various concentrations to first create a calibration curve showing the relationship between ethanol concentration and current reduction value. Then, perform the same operation as above using a sample solution containing ethanol of unknown concentration, find the current reduction value at this time, and read the ethanol concentration corresponding to that value using the calibration curve created earlier. You can know the ethanol concentration of
以上は１回の電流値測定毎に容器内の液を交換する所謂
バッチ方式の測定操作を説明したが。The so-called batch-type measurement operation in which the liquid in the container is replaced every time a current value is measured has been described above.
フローセルを用いた連続測定にも、本発明のエタノール
センサーが適用できることは勿論である。Of course, the ethanol sensor of the present invention can also be applied to continuous measurement using a flow cell.
［実 施 例］
（１）細胞膜画分の調製
培養した酢酸菌（Ｇｌｕｃｏｎｏｂａｃｔｅｒ ５ｕｂ
ｏｘｙｄａｎｓ丁Ｆ０１２５２８）の菌体を遠心分離（
５０００Ｘ Ｇ、 １０分）して集菌し、この菌体をフ
レンチプレスにより破砕した。この破砕物を超遠心分離
（６８０００Ｘ Ｇ、９０分）した後、沈殿を凍結乾燥
して細胞膜画分を得た。[Example] (1) Preparation of cell membrane fraction Cultured acetic acid bacteria (Gluconobacter 5ub)
The bacterial cells of P. oxydans F012528) were centrifuged (
5,000×G for 10 minutes) to collect the bacteria, and the cells were crushed using a French press. This crushed product was subjected to ultracentrifugation (68,000×G, 90 minutes), and then the precipitate was freeze-dried to obtain a cell membrane fraction.
（２） エタノールセンサーの製作
上記の如くして得た細胞膜画分２０ｍｇを、０．１Ｍ酢
酸緩衝液（ｐｔ＋５．５）１ｍｌに溶解し、この溶液を
多孔質高分子膜に注いで吸引濾過することにより、細胞
膜画分を多孔質高分子膜に吸着固定した。(2) Production of ethanol sensor 20 mg of the cell membrane fraction obtained as above was dissolved in 1 ml of 0.1M acetate buffer (pt+5.5), and this solution was poured onto a porous polymer membrane and filtered by suction. By this, the cell membrane fraction was adsorbed and fixed on the porous polymer membrane.
この場合の多孔質高分子膜には、東洋濾紙（株）製の多
孔質ニトロセルロースフィルター（７ｙｐｅＮＣ。In this case, the porous polymer membrane is a porous nitrocellulose filter (7ypeNC manufactured by Toyo Roshi Co., Ltd.).
直径２５ＩＩＩＩＩ＋、細孔径０．４５μｍ）を使用し
た。diameter 25III+, pore size 0.45 μm) was used.
次に、上記の固定化膜を石川製作所（株）製の０２電極
（Ｔｙｐｅ ＤＧ−５）上に装着した後、さらにその外
側をガス透過性膜で囲繞し、０リングで固定することに
より、本発明のエタノールセンサーを製作した。ガス透
過性膜として、本例ではミリボア社製の多孔質テフロン
膜（Ｔｙｐｅ ＦＨ，細孔径０．５μＩｎ）を使用した
。Next, after mounting the above-mentioned immobilization membrane on the 02 electrode (Type DG-5) manufactured by Ishikawa Seisakusho Co., Ltd., the outside of the membrane was further surrounded by a gas permeable membrane and fixed with an 0 ring. An ethanol sensor of the present invention was manufactured. In this example, a porous Teflon membrane (Type FH, pore diameter 0.5 μIn) manufactured by Millibore was used as the gas permeable membrane.
（３） エタノール濃度の測定
測定はエタノールセンサーを第１図に示す如く記録計４
に結線して行った。本例で使用した０２電極はアンペロ
メトリックな測定原理を利用しているので、出力は電流
イ直の変化として出力される。従って、第１図に示す如
く、端子間に１にΩの抵抗を入れることにより、電流値
を電圧値に変換して記録した。(3) Measurement of ethanol concentration The ethanol sensor is connected to the recorder 4 as shown in Figure 1.
I connected it to. Since the 02 electrode used in this example utilizes the amperometric measurement principle, the output is output as a change in the current I. Therefore, as shown in FIG. 1, by inserting a resistor of 1 to Ω between the terminals, the current value was converted into a voltage value and recorded.
１０ｍ１の水又は緩衝液を容器５に収めて恒温２５℃に
保持し、液をマグネテイツクスターラーで攪拌すること
により、溶存酸素を飽和状７１とした。これにエタノー
ルセンサーを挿入し、出力電流が安定した時の初期電流
Ｉｏを得る。次に、濃度既知のエタノール溶液１０μｍ
を注入すると、出力電流が変化し、約５分で定常状態に
なり、定常電流Ｉｓを得る。この時の電流減少値、すな
わちＩｏ−ＩｓをΔ丁とする。この操作を濃度既知のエ
タノール溶液について、エタノール濃度を変化させなが
ら繰り返すことにより、第２図に示すような検量線を作
成した。10 ml of water or a buffer solution was placed in a container 5 and maintained at a constant temperature of 25° C., and the solution was stirred with a magnetic stirrer to bring the dissolved oxygen to a saturated state of 71°C. An ethanol sensor is inserted into this to obtain the initial current Io when the output current is stabilized. Next, 10 μm of an ethanol solution with a known concentration
When injected, the output current changes and reaches a steady state in about 5 minutes to obtain a steady current Is. The current reduction value at this time, that is, Io-Is, is assumed to be Δd. By repeating this operation for ethanol solutions of known concentrations while changing the ethanol concentration, a calibration curve as shown in FIG. 2 was created.
この検量線から明らかな通り、濃度Ｏ〜３０ｍ１の範囲
で、エタノール濃度と電流減少値Δ■との間には、認め
られるのでこの検量線と上記したエタノールセンサーを
使用して、Ｓ度未知のエタノール溶液について、エタノ
ールの走破を行うことができた。As is clear from this calibration curve, there is a difference between the ethanol concentration and the current reduction value Δ■ in the concentration range from O to 30ml, so using this calibration curve and the above-mentioned ethanol sensor, For the ethanol solution, we were able to run ethanol.
［発明の効果］
本発明のエタノールセンサーは、アルコールデヒドロゲ
ナーゼを利用している関係で、メタノールに感応するこ
とがなく、エタノールに対してのみ選択的に感応する。[Effects of the Invention] Since the ethanol sensor of the present invention utilizes alcohol dehydrogenase, it is not sensitive to methanol and is selectively sensitive only to ethanol.
また、本発明で使用される酢酸菌由来のアルコールデヒ
ドロゲナーゼは、補酵素を必要としないので、電極の構
造が簡酢になり、容易に製作できる。さらに、ガス透過
性膜を付設した本発明のエタノールセンサーは、広いｐ
Ｈ域での使用が可能であるばかりでなく、測定液中に種
々の妨害物質が溶存している場合にも、その影響を受け
ない利点がある。従って、本発明のエタノールセンサー
は、血液や発酵液等の様々な物質が混在している試料液
のエタノール濃度を測定する際に、極めて有用である。Furthermore, since the alcohol dehydrogenase derived from acetic acid bacteria used in the present invention does not require a coenzyme, the electrode structure is simple and can be easily produced. Furthermore, the ethanol sensor of the present invention equipped with a gas permeable membrane has a wide p
Not only can it be used in the H range, but it also has the advantage of not being affected by various interfering substances dissolved in the measurement liquid. Therefore, the ethanol sensor of the present invention is extremely useful when measuring the ethanol concentration of a sample liquid containing various substances such as blood and fermentation liquid.
第１図は本発明のエタノールセンサーの説明図、第２図
は本発明のエタノールセンサーを用いてエタノール濃度
を測定する場合の検量線の一例である。
に０２電極 ２：固定化膜
３：ガス透過性膜 ４：記録計
５：恒温容器 ６：マグネテイツク７：Ｏリング
スターラー
特許出願人 朝日麦酒株式会社
１面
篤２図
エタノール ＬｍｇムηFIG. 1 is an explanatory diagram of the ethanol sensor of the present invention, and FIG. 2 is an example of a calibration curve when measuring ethanol concentration using the ethanol sensor of the present invention. 02 electrode 2: Immobilization membrane 3: Gas permeable membrane 4: Recorder 5: Constant temperature container 6: Magnetic 7: O-ring stirrer Patent applicant Asahi Beer Co., Ltd.
Claims (1)
して設けたことを特徴とするエタノールセンサー。1. An ethanol sensor characterized in that a cell membrane fraction of acetic acid bacteria is provided as an immobilized membrane on an O_2 electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61121833A JPS62277548A (en) | 1986-05-27 | 1986-05-27 | Ethanol sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61121833A JPS62277548A (en) | 1986-05-27 | 1986-05-27 | Ethanol sensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62277548A true JPS62277548A (en) | 1987-12-02 |
| JPH0416743B2 JPH0416743B2 (en) | 1992-03-25 |
Family
ID=14821057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61121833A Granted JPS62277548A (en) | 1986-05-27 | 1986-05-27 | Ethanol sensor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62277548A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5209275A (en) * | 1987-07-09 | 1993-05-11 | Junkosha Co., Ltd. | Liquid dispensing apparatus and method by sensing the type of liquid vapors in the receiver |
| US6740215B1 (en) | 1999-11-16 | 2004-05-25 | Matsushita Electric Industrial Co., Ltd. | Biosensor |
-
1986
- 1986-05-27 JP JP61121833A patent/JPS62277548A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5209275A (en) * | 1987-07-09 | 1993-05-11 | Junkosha Co., Ltd. | Liquid dispensing apparatus and method by sensing the type of liquid vapors in the receiver |
| US6740215B1 (en) | 1999-11-16 | 2004-05-25 | Matsushita Electric Industrial Co., Ltd. | Biosensor |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0416743B2 (en) | 1992-03-25 |
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| LAPS | Cancellation because of no payment of annual fees |