JPS62263468A - Ink composition and examination body for detecting glucose - Google Patents

Abstract

PURPOSE:To obtain an ink composition capable of forming a glucose examination body having excellent sensitivity and quantification capacity, by dissolving or dispersing a reagent composition consisting of glucose oxidase, peroxidase, an oxidizable indicator, a sensitivity controller, a stabilizer, a pH buffer, a binder and a water absorbable powder in a non-aqueous solvent. CONSTITUTION:Glucose oxidase is used in a state of a purified freeze-dry product and peroxidase is enzyme catalyzing the oxidation of various org. substances due to hydrogen peroxide and an oxidazable indicator forms a color by oxidation. Further, the linearity of the concn. of glucose contained in a specimen and the coloration density of an examination reagent part is improved by a sensitivity controller and a stabilizer contributes to the stabilization of a reagent composition and a pH buffer is used in order to hold a pH value near to pH allowing the oxidizable indicator to generate pref. color change. A binder must have a property exerting no effect on the component in a specimen solution and pH of said solution and a water absorbable powder is added in order to develop function for enhancing the water absorbability of the reagent composition.

Description

[Detailed description of the invention]

[Industrial Application Field 1] The present invention relates to an ink composition suitable for forming a test body capable of easily and quickly detecting glucose in a solution, particularly a body fluid such as urine, and a test body formed using the ink composition. Regarding. [Prior art 1: Quickly and easily detecting glucose levels in body fluids such as urine, blood, or lymph fluid is useful for detecting the ψ stage of urinary disease 1
Essential for diagnosis and management. To detect glucose in body fluids, particularly in the home, test specimens coated with glucose test reagents have conventionally been used. This test specimen had the advantage of being easy to operate and allowing determination to be made in a short time. By the way, in a test sample for detecting glucose in body fluids, glucose reacts with oxygen in the air due to the action of glucose oxidase, and is finally oxidized to gluconic acid and hydrogen peroxide. This hydrogen peroxide produces nascent oxygen by the action of peroxidase, and this oxygen immediately reacts with an oxidizable indicator such as guaiac butter or o-tolidine to cause the indicator to develop color. A test sample for detecting glucose in body fluids using this principle is
After dissolving or dispersing a reagent composition consisting of 1 g of oxidase, peroxidase, and an oxidizable indicator in water or a water-alcoholic solvent, and impregnating a mouthpiece with the resulting liquid,
After drying, the opening paper is pasted onto a plastic film and cut to an appropriate size. However, this method has the following problems. (a) When a reagent composition consisting of sugar oxidase, peroxidase, and an oxidizable indicator is dissolved or dispersed in water or a water-alcoholic solvent to prepare an impregnating solution, the enzyme is unstable and easily deactivated. Moreover, the impregnating liquid deteriorates rapidly. For this reason, it is necessary to impregnate the opening paper in multiple steps immediately after preparing the impregnating liquid. Furthermore, even if the paper is impregnated immediately, there is a problem that some of the enzymes are deactivated and the impregnating liquid is partially deteriorated. (b) As mentioned above, since the impregnating liquid is unstable and the manufacturing process is complicated, it is difficult to maintain a constant quality of the test specimen obtained, and it is necessary to ensure the test accuracy and reliability of the test specimen. requires special care and skill, tends to reduce manufacturing efficiency, and increases manufacturing costs. For this reason, the development of test specimens that can simplify the manufacturing process and are suitable for mass production is progressing, and in Japanese Patent Publication No. 44-25953, enzymes are predissolved in a water-alcohol mixed solution.
An indicator, a pH buffer, a polymeric binder, a water-absorbing carrier, etc. are mixed with this to prepare an ink composition suitable for printing or coating, and this ink composition is printed (coated) on a support. A method has been proposed in which the test specimen is manufactured by drying the test specimen. However, in this method, the enzymes are partially dissolved in water and therefore unstable and rapidly deactivated, so it was necessary to print immediately after producing w4. Furthermore, it is necessary to dry at a low temperature to prevent deactivation of the enzyme, and furthermore, in order to obtain long-term storage stability, it is necessary to minimize the amount of residual moisture in the coated reagent layer. Under these circumstances, the present applicant filed the
No. 9995, an ink composition is prepared by dispersing enzymes in a nonaqueous solvent that hardly dissolves the enzymes, and then further dissolving or dispersing an indicator, a buffer, a binder, and a water-absorbing carrier. We proposed a method for manufacturing test specimens by printing a composition on a support. According to this method, a test specimen that exhibits excellent quantitative performance and sensitivity for glucose can be obtained, but when this test specimen is exposed to the atmosphere for a long time, the color gradually becomes unrestricted and unclear. In addition, high-concentration coloring occurs and becomes saturated while the glucose concentration in the sample is low, making it difficult to quantify except for very low concentrations of glucose. For this reason, when testing body fluids, there is a risk of incorrect judgment being made, and a solution to these problems has been strongly desired.

[Problem to be solved by the invention 1] As a result of the inventors' research to solve the above problems,
The decrease in color uniformity and sharpness in the above-mentioned glucose detection test strip is caused by the presence of trace amounts of the oxidizable indicator in the reagent composition, especially the oxidizable indicator contained therein, which is affected by filtration oxidizing substances, etc. I found out that this is due to this. As a result of further research, by adding a sensitivity regulator and a stabilizer to the reagent composition, the proportional relationship between the concentration of grape 'Is in the sample and the color density was improved, and the concentration of glucose from low to high concentration was improved. It was found that the color density increases linearly over time, quantification becomes easier, and the color uniformity and sharpness are also good.Furthermore, by appropriately selecting the oxidizable indicator, more favorable results can be obtained. The present invention seeks to solve the drawbacks associated with the prior art and has the following objects: (a) glucose detection with excellent sensitivity and quantitative performance; To provide an ink composition capable of forming a body and a glucose detector formed using the same. (b) An ink composition for glucose detection that is stable even when stored in the atmosphere for a long time and does not cause discoloration. To provide an ink composition and a glucose detector formed using the ink composition. To provide an ink composition for detecting glucose in body fluids according to the present invention.
A reagent composition consisting of a sugar oxidase, peroxidase, an oxidizable indicator, a sensitivity regulator, a stabilizer, a pH buffer, a binder, and a water-absorbing powder is dissolved or dispersed in a non-aqueous solvent. . Further, the glucose detector according to the present invention has a glucose detection area formed by coating a glucose detection ink composition having the above composition on a support. Glucose in body fluids reacts with oxygen in the air through the action of glucose oxidases such as glucose oxidase, and is finally oxidized to gluconic acid and hydrogen peroxide. The generated hydrogen peroxide produces nascent oxygen by the action of peroxidase, and this oxygen immediately reacts with an oxidizable indicator such as guaiac butter or O-tolidine to cause the indicator to develop color. Based on the degree of color development, the presence or absence of glucose in the body fluid and its amount are determined semi-quantitatively. Each component constituting the ink composition for glucose detection used in forming the test object according to the present invention will be described in detail below. B) Glucose oxidase Glucose oxidase as a sugar oxidase is used in the form of a purified freeze-dried product. For example, when this enzyme is used with a titer of 100 units/ff1g, the enzyme activity is 0.0% based on the solid content of the ink composition.
It is desirable to be present in an amount of 2 to 2% by weight, preferably 0.2 to 1.8% by weight. Peroxidase Peroxidase is an enzyme that oxidizes various minerals with hydrogen peroxide or organic peroxide, and is mainly extracted from horseradish. For example, when a freeze-dried product with a titer of 100 units/Rg is used, this enzyme is present in an amount of 0.002 to 1% by weight, preferably 0.02 to 0.2% by weight based on the solid content of the ink composition. It is desirable that it be present in the range of %. C) Oxidizable Indicator The oxidizable indicator is an indicator that develops color when oxidized by oxygen, and conventionally known compounds such as benzidines and N-alkylated benzidines can be widely used. Fat. O-tolidine is preferred, and among these, it is particularly preferred to use guaiac butter. By using guaiac butter as an oxidizable indicator and also using a sensitivity adjusting agent described below, the ink composition for glucose detection and the test material for glucose detection are more effective than when using a commonly used benzidine derivative. Safety, storage stability, and color stability are improved. The oxidizable indicator is preferably 0.5 to 10% by weight based on the solid content of the ink composition, preferably 0.6 to 6% by weight.
Preferably, it is present in weight percentages. 2) Sensitivity regulator In the present invention, by using an aliphatic carboxylic acid ester of ascorbic acid as a sensitivity regulator, it is possible to improve the quantitative properties of a test sample for glucose detection, that is, the glucose concentration contained in the sample and the test reagent portion. It is possible to improve linearity with color density. If an aliphatic carboxylic acid ester of ascorbic acid is not added, a high 21:1 coloration occurs while the glucose concentration in the sample is low and becomes saturated, making it difficult to quantify except for very low concentrations of glucose. is difficult. Preferred examples of the aliphatic carboxylic acids include aliphatic saturated carboxylic acids having a total number of saturated carbon atoms of CC, particularly C12 to C2°. Lower carboxylic acid esters of ascorbic acid are easily soluble in water, and those with 022 or higher are compatible with other components, WJ
It is not preferred due to its solubility in agents and its low sensitivity adjustment effect compared to its weight. The added layer of the sensitivity modifier is based on the solid content of the ink composition',
0.02 to 5% by weight is desirable. e) Stabilizer Stabilizers include sugar oxidase, peroxidase, oxidizable indicator, sensitivity regulator, p■ buffer, and binder. and contributes to stabilizing the reagent composition consisting of the water-absorbing powder. Among these, oxidizable indicators have a tendency to change color due to the action of peroxides in the atmosphere, as described above, and the main role of the stabilizer is to prevent this. The fatty acid ester of ascorbic acid, which is the sensitivity regulator, also functions as a stabilizer. Reducing agents such as ascorbic acid have the property of inhibiting the reaction system for glucose detection, which is the purpose of this test, that is, the oxidation reaction of the oxidizable indicator, resulting in a decrease in sensitivity. By selectively using an aliphatic saturated carboxylic acid having a carbon number of C1 to C3o, it is possible to avoid coloration inhibition of the oxidizable indicator caused by the aliphatic carboxylic acid ester of ascorbic acid. Therefore, it is possible to use an aliphatic ester of ascorbic acid as a stabilizer to prevent oxidation of the oxidizable indicator during storage of a glucose detection specimen. , a compound having antioxidant activity, a specific surfactant typified by glycerol esters, or a mixture thereof may be added as appropriate. The layer to which these stabilizers are added is not particularly limited, but preferably 0.02 to 5% by weight based on the solid content of the ink composition. to) all! The agent pH11 is used to maintain a pH value close to the pH at which the oxidizable indicator causes a preferable color change in the ink composition for detecting glucose. The pea WiI agent may be any agent as long as it can impart a predetermined pH value (for example, pH 5>) to the ink composition, and specifically, a combination of citric acid and sodium citrate is preferably used. G) Binding agent The binding agent should be one that does not affect the components in the sample body fluid and I)H, etc., and does not affect the reagents, especially enzymes and oxidizable indicators, and does not interfere with the color reaction. something is required. Binders that have been confirmed to meet these requirements include (I> polyester resins, alkyd resins, polyurethane resins, polystyrene resins; 9. acrylic resins; epoxy resins, vinyl chloride resins, vinyl chloride copolymers; 4.
1rl resin, polyvinyl butyral resin, polyvinyl alcohol resin, polyvinylpyrrolidone resin. Synthetic resins such as maleic anhydride copolymers, (I) Cellulose derivatives such as methylcellulose, ethylcellulose, hydroxyethylcellulose, and carboxymethylcellulose, (II) Natural polymers such as starch, polysaccharides, gelatin, casein, or sodium alginate. etc. are used. Furthermore, two or more of these binders may be combined. This binder is 0.1% based on the solid content of the ink composition.
It is desirable to be present in an amount of ~20% by weight, preferably 0.5-10% by weight. H) Water-absorbing powder Addition of the water-absorbing powder increases the water absorption of the reagent composition provided on the support, promotes contact between the target liquid and the reagent composition, and promotes the coloring reaction of the indicator. Such a water-absorbing powder that has the function of:
Those exhibiting extreme acidity or alkalinity are not preferred, and those with high whiteness are preferred. Specifically, kaolin, synthetic silica, glass, cellulose block, microcrystalline cellulose, ion exchange cell O-su, ion exchange resin, calcium carbonate, magnesium carbonate, aluminum silicate, etc. can be used. The water-absorbing powder has a content of 30 to 9 based on the solid content of the ink composition.
Preferably it is present in an amount of 0% by weight. B) Non-aqueous solvent Each of the above components is dissolved or dispersed in a non-aqueous solvent that does not substantially contain water. Examples of such non-aqueous solvents include (a) aromatic hydrocarbons such as benzene and toluene;
b) Aliphatic ketones such as methyl ethyl ketone, (C) esters such as ethyl acetate, or (d) alcohols such as n-butanol are used. Among alcohols,
C-C2 lower alcohols are not preferred because they lead to deactivation of 8. It is preferable that the non-aqueous solvent does not substantially contain water, and therefore it is preferable that the solvent is dehydrated before use. n) Other components In addition to the above-mentioned components, a humectant may be added to the glucose detection ink composition depending on the case. As wetting agents, nonionic surfactants, anionic surfactants, cationic surfactants, zwitterionic surfactants, polyethylene glycols, etc. are used, and these wetting agents include:
It helps in dispersing each reagent, promotes the formation of a uniform reagent layer, and improves water wettability. The wetting agent is preferably present in an amount of 0.5 to 5% by weight based on the solids content of the ink composition. Further, in order to make the color tone of the indicator more visible, a background dye such as oil yellow may be added. Note that when a glucose detection area is formed using the glucose detection ink composition having the composition described above to detect glucose in a body fluid, reducing substances such as ascorbic acid, glutathione, or cysteine coexist in the body fluid. There is also the advantage that, even when these substances are present, there is hardly any adverse effect on the color reaction caused by these substances. The glucose detection ink composition as described above is applied onto a support to form a glucose detection area, thereby obtaining a test object according to the present invention. Application techniques include printing methods, coating methods (e.g. roll coating, spray coating, dip coating, solid coating)
etc. can be used. In the present invention, since it is preferable that the amount of the ink composition applied is relatively large and constant, silk screen printing method and intaglio printing method are used. Preferably, the ink composition is provided on the support by a gravure printing method or the like. The coating amount varies depending on the type of ink composition, but is generally preferably 2 to 150 g/m<dry. The support is preferably one that does not react with the reagent composition and does not inhibit the coloring of the reagent, and specifically, for example, paper 2 synthetic paper, nonwoven fabric, synthetic resin film, or a combination of paper and synthetic resin film. A laminate or the like is used. The test body according to the present invention, in which a glucose test area is provided on such a support, may be formed into a stick shape, a roll shape, a tape shape, or the like. Alternatively, the support itself may be formed into a shape capable of collecting the body fluid to be tested, such as a pot shape, a test tube shape, a tray shape, or a dropper shape, and a glucose test area may be provided on the support body, according to the present invention. It may also be used as a test object. (Example) The present invention will be explained below with reference to Examples, but the present invention is not limited to these Examples. Example 1 Glucose detection ink having the following composition. After dispersing, the thickness is 30 mm by screen printing.
It was printed on a 0μ white polystyrene sheet so that each side was a 5M square. The screen plate used was 80 mesh, and the total thickness of the resist and screen gauze was 130μ.
Met. Ink composition for glucose detection Grape WM convertase (manufactured by Toyobo; Grade fl)
3.6 parts by weight peroxidase (manufactured by Toyobo; Grade m >2
．． 4 parts by weight Guaac butter 4.8 parts by weight Sorbitan monolaurate (manufactured by Kao Soap; Span 20) 7.2 parts by weight 1 to 0.48 parts by weight citric acid 2.8 parts by weight Sodium citrate 11. Offfffi part Polyvinylpyrrolidone (manufactured by BASF; Koliton 90) 12.6
Parts by weight Polyvinyl butyral (Sekisui Chemical Co., Ltd.: S-LEC BX-1) 2.25 parts by weight Cellulose fine powder (Kyakasei Co., Ltd.: Avicel SF) 171 Parts by weight n-amyl alcohol 228 Parts by weight Butyl cellosolve acetate 33.5 11 parts obtained The printed matter was dried at 60° C. for 40 minutes and then cut into sticks to obtain test pieces for glucose detection. 50q/dl of β-D-glucose in normal urine and normal urine
, 100I#g/old, 250■/d+,
The sticks were dissolved to concentrations of 500q/old and 2000ay/old, and the sticks were taken out immediately after immersion, left to stand for 1 minute, and the color tone of the test reagent portion was observed. The color of the test reagent part is uniform and bright, and the color density is 1! It increased stepwise as the laI degree increased, and the glucose concentration in the sample could be clearly determined within the above range. No change in color tone was observed even when the colored specimen was allowed to stand at room temperature for 5 minutes. The obtained specimen was sealed in a glass container and heated at 40°C for 12
After storage for several months, a test was conducted in the same manner as above, and the color of the test reagent was uniform, clear, and stable as before storage, and the concentration of buto-91g in the sample was the same as before storage. It was clearly distinguishable. Comparative Example 1 A test specimen for glucose detection was obtained in the same manner as in Example 1 using the same ink composition for glucose detection as in Example 1 except that the ascorbyl stearate shown in Example 1 was not used. Normal urine and normal urine with β-D-glucose at 5ormg/
Old, 100q/old, 250IRy/old, 5
The sticks were dissolved to a concentration of 0h+y/old and 2000q/dI and used as specimens, and the sticks were taken out immediately after immersion, left to stand for 1 minute, and the color tone of the test reagent portion was observed. The coloration of the test reagent part was uniform and clear as in Example 1. Moreover, it was stable. However, when the glucose concentration in the sample is in the range of 50 μ/di to 250 IRy/dl, the color density increases rapidly as the glucose concentration increases.
In addition, when the glucose concentration in the sample is in the range of 250I#g/dl to 20007IIF/dI, the color density gradually increases as the glucose concentration increases, and the glucose concentration in the sample can be clearly determined within the above range. It was difficult to distinguish. In addition, the obtained specimen was sealed in a glass container and heated to 40°C.
After storage for 6 months, a test was conducted in the same manner as above, and the color of the test reagent part became unrestricted and unclear compared to before storage, and it was difficult to distinguish the 31% glucose in the sample. Ta. Example 2 A specimen for glucose detection was prepared in the same manner as in Example 1 using the ink composition for glucose detection having the composition shown below. Ink composition for glucose detection Glucose oxidase (manufactured by Toyobo: Grade II)
3.6 Parts by weight Peroxidase (manufactured by Toyobo; Grade M) 2
．． 4 parts by weight Guaiac butter 4.8 parts by weight Sorbitan monolaurate (Kao Soap ¥J Nispan 20) 7.2 parts by weight Ascorbyl palmitate 0.48 parts by weight Citric acid 2.8 parts by weight Sodium citrate 11. Part OII: 7 methyl alcohol ester of methyl vinyl ether/anhydride-inic acid copolymer (manufactured by G, A, F companies: Gantrez AN-169) 7.0 parts by weight Cellulose fine powder (manufactured by Fukaisei; Avicel SF) 171 Parts by weight n-amyl alcohol 228 Parts by weight Putilce O-solve acetate 33.5 Parts by weight Normal urine and β-D-glucose in normal urine 501#g/dl
, 100119/dl, 250IRg/dl, 5
00IIlil/dl and 2000119/old were used as specimens, and the sticks were taken out immediately after immersion, left to stand for 1 minute, and the color tone of the test reagent portion was observed. The coloration of the test reagent portion was uniform and clear, and no change in color tone was observed even when the colored test specimen was left standing at room temperature for 5 minutes. Furthermore, as in Example 1, the color density increased stepwise as the glucose concentration in the sample increased, making it possible to discriminate the glucose concentration in the sample. The obtained specimen was sealed in a glass container and incubated at 40°C for 12
After storage for several months, a test was conducted in the same manner as above, and the coloration of the test reagent portion was as uniform, clear, and stable as before storage. Comparative Example 2 A test specimen for glucose detection was obtained in the same manner as in Example 2 using the same glucose detection ink composition as in Example 2 except that ascorbyl palmitate was not used. . Using a sample prepared in the same manner as in Example 2, the stick was taken out immediately after immersion, left to stand for 1 minute, and the color tone of the test reagent portion was observed. The coloration of the test reagent body was uniform and clear, and no change in color tone was observed even when the colored test body was allowed to stand at room temperature for 5 minutes. However, as described in Comparative Example 1, it was difficult to determine the glucose concentration in the sample. The obtained specimen was sealed in a glass container and stored at 40°C for 6 months, and then tested in the same manner as above.
Compared to before storage, the coloration of the test reagent part became unrestricted and unclear. Example 3 A test specimen for glucose detection was obtained in the same manner as in Example 1 using an ink composition for glucose detection having the following composition. Ink composition for glucose detection Glucose oxidase (manufactured by Toyobo; Grade ■) 3.
6 Heavy Φ peroxidase (manufactured by Toyobo; Grade II [)
2.4 Parts by weight 0-Tolidine 10.0 Parts by weight Sorbitan monolaurate (Nispan 20 manufactured by Kao Soap) 7.2 Parts by weight Ascorbyl stearate 0.5 Parts by weight Citrus M
2.8 Heavy Sodium Citrate 11. Offflffl part: Amyl alcohol ester of methyl vinyl ether/maleic anhydride copolymer (manufactured by G, A, F companies; Gauntletz N-169) 7.0 parts by weight Cellulose fine powder (manufactured by Fukaisei: Avicel SF) R7 parts by weight n-
7 mil alcohol 228 parts by weight Butyl cellosolve acetate 33.5 parts by weight Normal urine and β-D-glucose in normal urine 50ffig/dl,
1100I1/dl, 250IRg/dl, 500
The sample was dissolved to a concentration of 1 g/dl and 2000 ml/di, and the stick was taken out immediately after immersion, left to stand for 1 minute, and the color tone of the test reagent portion was observed. The coloration of the test reagent part is uniform and clear, and the color density increases stepwise as the glucose concentration in the sample increases.
It was possible to clearly determine the glucose concentration within the above range. When the colored specimen was allowed to stand still for 1 minute, the color density decreased significantly. The obtained specimen was sealed in a glass container and stored at 40°C for 6 months, and then tested in the same manner as above.
The coloration of the test reagent portion was uniform, clear, and stable compared to before storage, and the glucose concentration in the sample was also clearly distinguishable as before storage. Comparative Example 3 A test specimen for glucose detection was colored blue in the same manner as in Example 1 using the ink composition for glucose detection having the composition shown below. Ink composition for glucose detection Glucose oxidase (manufactured by Toyobo; Grade ■) 3.
6 parts by weight peroxidase (manufactured by Toyobo; Grade l[)2
．． 4 parts o-tolidine io, o parts by weight Sorbitan monolaurate (manufactured by Hanatamaishi υ; Span 20) 7.2 parts by weight citric acid
2.8 parts by weight sodium citrate 11. Oli part: Medemethyl vinyl ether/indic acid anhydride copolymer <manufactured by GAF; Gauntletz N-169) amyl alcohol esterified product 7.0 parts by weight Cellulose fine powder (manufactured by Renkasei; Avicel SF) 171 Heavy suspension part - Amyl alcohol: 228 parts by weight Butyl cellosolve acetate: 33.5 parts by weight Using a specimen prepared in the same manner as in Example 3, the stick was immediately taken out after soaking for 81 minutes, left to stand for 1 minute, and the color tone of the test reagent portion was observed. Immediately after taking out the test reagent from the immersion liquid, the coloration of the test reagent portion was uniform and clear, but when the colored test sample was allowed to stand at room temperature for 1 minute, the color density decreased significantly. Grape U concentration in the sample is from 501#g/d+ to 25
In the range of 0q/d1, the color density is Grape I! When the concentration of glucose in the sample ranges from 250q/dI to 2000/IjiF/Old, the color concentration gradually increases as the concentration of glucose increases, and the above It was difficult to clearly determine the glucose concentration in the sample within this range. The obtained specimen was sealed in a glass container and stored at 40°C for one month, and then tested in the same manner as above.
Compared to before storage, the coloration of the test reagent part became noticeably uneven and unclear.

【Effect of the invention】

The ink composition for glucose detection according to the present invention and the specimen formed using the same can be used to detect glucose as described in the above Examples by using an aliphatic carboxylic acid ester of ascorbic acid as a sensitivity adjusting agent. Improvement in the quantitative performance of the detection test sample, that is, the proportional relationship between the glucose concentration in the sample and the color density of the test reagent part has been improved, and the glucose concentration in the sample can be linearly measured from low to high concentrations. The degree of color development increases, making quantitative determination easier. As described in the comparative example, when the aliphatic carboxylic acid ester of 7-scorbic acid is not added, a high concentration of coloration occurs while the glucose concentration in the sample is low, and no color change occurs in the range of high glucose concentration. Since the amount is extremely small, it is difficult to quantify except at extremely low glucose concentrations. In addition, since the aliphatic carboxylic acid ester of ascorbic acid also has the effect as a stabilizer, it is stable even when the ink composition for glucose detection and the test specimen are stored in the atmosphere for a long time. No discoloration phenomenon is observed. Furthermore, the glucose detection test body according to the present invention is suitable for mass production, and the manufacturing process can be simplified, since the glucose detection region can be formed on the support by direct coating, especially by printing.

Claims (6)

[Claims] (1) A reagent composition consisting of a sugar oxidase, peroxidase, an oxidizable indicator, a sensitivity regulator, a stabilizer, a pH buffer, a binder, and a water-absorbing powder is dissolved or dispersed in a non-aqueous solvent. An ink composition for glucose detection characterized by the following. (2) The ink composition for glucose detection according to claim 1, wherein the sensitivity regulator is an aliphatic carboxylic acid ester of ascorbic acid. (3) The ink composition for glucose detection according to claim 1, wherein the oxidizable indicator is guaiac butter. (4) A reagent composition consisting of a sugar oxidase, peroxidase, an oxidizable indicator, a sensitivity regulator, a stabilizer, a pH buffer, a binder, and a water-absorbing powder is dissolved or dispersed in a non-aqueous solvent. A test body for detecting glucose, which is obtained by applying an ink composition for detecting glucose onto a support. (5) The test material for glucose detection according to claim 4, wherein the sensitivity regulator is an aliphatic carboxylic acid ester of ascorbic acid. (6) The test material for glucose detection according to claim 4, wherein the oxidizable indicator is guaiac butter.