JPS62263193A - Water-soluble extract derived from aloe - Google Patents
Water-soluble extract derived from aloeInfo
- Publication number
- JPS62263193A JPS62263193A JP61107505A JP10750586A JPS62263193A JP S62263193 A JPS62263193 A JP S62263193A JP 61107505 A JP61107505 A JP 61107505A JP 10750586 A JP10750586 A JP 10750586A JP S62263193 A JPS62263193 A JP S62263193A
- Authority
- JP
- Japan
- Prior art keywords
- aloe
- liquid
- water
- leaves
- stems
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000011399 aloe vera Nutrition 0.000 title claims abstract description 43
- 241001116389 Aloe Species 0.000 title claims abstract description 41
- 239000000284 extract Substances 0.000 title claims abstract description 19
- 125000000837 carbohydrate group Chemical group 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 26
- 239000007788 liquid Substances 0.000 abstract description 25
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract description 9
- 239000002537 cosmetic Substances 0.000 abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 8
- 150000001720 carbohydrates Chemical class 0.000 abstract description 8
- 239000000287 crude extract Substances 0.000 abstract description 8
- 239000000706 filtrate Substances 0.000 abstract description 4
- 230000007062 hydrolysis Effects 0.000 abstract description 4
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 3
- 235000013402 health food Nutrition 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000012141 concentrate Substances 0.000 abstract description 2
- 230000001376 precipitating effect Effects 0.000 abstract description 2
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 description 20
- 150000004676 glycans Chemical class 0.000 description 15
- 229920001282 polysaccharide Polymers 0.000 description 15
- 239000005017 polysaccharide Substances 0.000 description 15
- 239000000843 powder Substances 0.000 description 15
- 229920002678 cellulose Polymers 0.000 description 10
- 239000001913 cellulose Substances 0.000 description 10
- 235000000346 sugar Nutrition 0.000 description 7
- 150000008163 sugars Chemical class 0.000 description 7
- 235000013305 food Nutrition 0.000 description 6
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 5
- 150000004056 anthraquinones Chemical class 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- AFHJQYHRLPMKHU-XXWVOBANSA-N Aloin Natural products O=C1c2c(O)cc(CO)cc2[C@H]([C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)c2c1c(O)ccc2 AFHJQYHRLPMKHU-XXWVOBANSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229940069521 aloe extract Drugs 0.000 description 3
- CPUHNROBVJNNPW-UHFFFAOYSA-N aloin A Natural products OC1C(O)C(O)C(CO)OC1OC1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 CPUHNROBVJNNPW-UHFFFAOYSA-N 0.000 description 3
- AFHJQYHRLPMKHU-WEZNYRQKSA-N aloin B Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@H]1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 AFHJQYHRLPMKHU-WEZNYRQKSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- AFHJQYHRLPMKHU-UHFFFAOYSA-N isobarbaloin Natural products OC1C(O)C(O)C(CO)OC1C1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 AFHJQYHRLPMKHU-UHFFFAOYSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 235000002961 Aloe barbadensis Nutrition 0.000 description 2
- YDQWDHRMZQUTBA-UHFFFAOYSA-N Aloe emodin Chemical compound C1=CC=C2C(=O)C3=CC(CO)=CC(O)=C3C(=O)C2=C1O YDQWDHRMZQUTBA-UHFFFAOYSA-N 0.000 description 2
- 244000186892 Aloe vera Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000013065 commercial product Substances 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000003957 anion exchange resin Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 235000020845 low-calorie diet Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- -1 tetraose Chemical class 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 150000003641 trioses Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Abstract
Description
【発明の詳細な説明】
〔イ〕 発明の目的
本発明は、アロエの茎葉の有効利用に関するものであり
、その要旨は、新鮮なアロエ茎葉をもとに、これより加
水分解を主体として得られる、分子量が１万以下の糖類
からなる抽出物に関する。[Detailed Description of the Invention] [A] Purpose of the Invention The present invention relates to the effective use of aloe stems and leaves. , relates to an extract consisting of saccharides with a molecular weight of 10,000 or less.
１産業上の利用分野」
本発明によるアロエの茎葉を出発原料として得られたｗ
Ｍは、水に溶解性が良好であり、溶解後の液は沈澱する
ことなく、化粧料（化粧品類全般）をはじめ、健康食品
や飲料などに、配合して用いることが出来る。1. Field of Industrial Use” W obtained using aloe stems and leaves as a starting material according to the present invention
M has good solubility in water, and the solution after dissolution does not precipitate and can be blended into cosmetics (cosmetics in general), health foods, drinks, and the like.
１従来の技術。1 Conventional technology.
アロエの茎葉を出発原料となし、これより得られる、さ
まざまな抽出物は、すでに医薬品をはじめ、化粧品や食
品（飲料）などに利用されてきている。Aloe vera stems and leaves are used as the starting material, and various extracts obtained from this have already been used in pharmaceuticals, cosmetics, and foods (drinks).
それを具体的に示す文献としては、例えば、次の刊行物
に示されている。Examples of documents specifically showing this are shown in the following publications.
ニューフードインダストリー ＶＯｌ、２６ ！１＆
１４ｐ、３０〜３６：食品資材研究会発行（１９８４）
フレグランスジャーナル Ｎ１１６０ （ＶＯｌ、ｌ
ｌ侮３）ｐ、１０７〜１１４：フレグランスジャーナ
ル社発行 （１９８３）
従来のアロエ茎葉の抽出物は、これを大別すれば、アワ
イン等々のアントラキノン系成分を主体にする抽出物と
、多糖体を主体とする抽出物に大別されるが、さらに、
全般的なアロエの従来の技術的な手段と、その用途につ
いてみれば、次のごシ ｌ −ｒＩ 諷 ス
アロエの新鮮な茎葉は、大量の水分を保持しており、そ
の茎葉の保水性能は、内部のゲル状組織繊維質により維
持されている。又、外皮は硬い繊維質で覆われており、
従来、この内部のゲル状組織に含む多量の液をしぼり、
これより、多種の抽出物が得られてきた。New Food Industry Vol. 26! 1&
14p, 30-36: Published by Food Materials Research Group (1984)
Fragrance Journal N1160 (VOl, l
3) p., 107-114: Published by Fragrance Journal (1983) Conventional extracts of aloe stems and leaves can be roughly divided into extracts containing mainly anthraquinone components such as awine, and extracts containing polysaccharides. It is broadly classified into the main extracts, but in addition,
Looking at the conventional technical means of aloe in general and its uses, the following is a summary of the following. It is maintained by an internal gel-like tissue fibrous substance. In addition, the outer skin is covered with hard fibers,
Conventionally, a large amount of liquid contained in this internal gel-like tissue was squeezed out,
From this, various types of extracts have been obtained.
つまり、しぼった液中には、樹脂質、多糖体をはじめ、
アントラキノン系の色素成分などが含まれ、これを加熱
乾固させたものは、日周アロエ、又、それを粉砕したも
のは、日周アロエ末として収載されている。In other words, the squeezed liquid contains resins, polysaccharides, etc.
It contains anthraquinone-based pigment components, etc., and the product obtained by heating and drying this is listed as diurnal aloe, and the crushed product is listed as diurnal aloe powder.
このアロエの新鮮な茎葉のしぼり液は、常温では急速に
褐変化が進むと共に、沈澱が進み、この際、一旦、沈澱
形成された成分は、高分子な多糖体が主体であり、再び
水には溶解しない性質を有している。At room temperature, this squeezed liquid from fresh stems and leaves of aloe rapidly browns and begins to precipitate.At this time, once the precipitate is formed, the components that have formed are mainly high-molecular polysaccharides, which are then rehydrated in water. has the property of not dissolving.
具体的には、従来の食用又は、化粧料用に用いられてき
たところの抽出物について述べれば、上述の沈澱物を除
去したところのしぼり液中には、黄色系の色素成分であ
る、アントラキノン系色素（アロイン、アロエエモジン
）などと共に、水に可溶な多糖体が主体に含まれており
、これを、そのまま精製した液体（例えば市販品名ニア
ローケープリキッド）、ξらに、両成分を、それぞれ分
取して、アロインを主体に精製した液体（例えば市販品
名ニアローケープリキッドＢ−７）、水に可溶な多糖体
を主体に精製した無色透明な液体（例えば市販品名：ア
ロエベラリキッド）などがある。Specifically, regarding extracts that have been conventionally used for food or cosmetics, the squeezed liquid after removing the above-mentioned precipitate contains anthraquinone, a yellow pigment component. It mainly contains polysaccharides that are soluble in water, along with other pigments (aloin, aloe emodin), etc., and both components are added to a purified liquid (for example, commercially available product name: Near Cape Liquid), ξ, etc. A liquid purified mainly from aloin (e.g. commercial product name: Near Cape Liquid B-7) and a colorless and transparent liquid purified mainly from water-soluble polysaccharides (e.g. commercial product name: Aloe Vera Liquid) and so on.
一方、上述のアロエ茎葉からのしぼり液中の沈゛ 澱物
には、水に不溶性の高分子の多糖体の他に、アント・ラ
キノン系の成分などが含まれているが、脱色等々の処理
を加えると共に、アントラキノン系成分や樹脂質を除去
して、白色状の多糖体を主体とする粉末が得られている
。この粉末は、我が国では、通常、アロエホワイトパウ
ダーとも称し、水には、はとんど溶解しない欠点があっ
た。On the other hand, the precipitate in the above-mentioned squeezed liquid from aloe stems and leaves contains anthraquinone-based components in addition to water-insoluble polymeric polysaccharides; At the same time, anthraquinone components and resins are removed to obtain a white powder mainly composed of polysaccharides. This powder is commonly called Aloe White Powder in Japan, and has the disadvantage that it hardly dissolves in water.
したがって、化粧品類においては、クリームや乳液中に
、他の分散剤と共に併用して用いるケースもあった。こ
のアロエホワイトパウダーは、アロエセルロースとも呼
び、アロエの茎葉中に含まれる水分保留能を有する、ゲ
ル組繊細胞を構成する多糖体ｍ維質を主体とする抽出物
のことである。Therefore, in cosmetics, it has sometimes been used in combination with other dispersants in creams and emulsions. This aloe white powder is also called aloe cellulose and is an extract mainly composed of polysaccharide m-fiber that constitutes gel fiber cells and has the ability to retain water contained in aloe stems and leaves.
このゲル組織を構成する多糖体成分は、多量に含まれる
アロエ葉中の水分存在下では、溶解性を示しているも、
一旦、圧搾などを行ない、その葉に含まれる水分を除去
すると、これにともなって、水にはまったく不溶となっ
て沈澱する。このような状態では、もはや肌や化粧品自
体（製品）の保湿、湿潤性能も発揮しないものとなって
しまう。Although the polysaccharide components that make up this gel tissue are soluble in the presence of a large amount of water contained in aloe leaves,
Once the water contained in the leaves is removed by squeezing, etc., the leaves become completely insoluble in water and precipitate. In such a state, the moisturizing and moisturizing properties of the skin and the cosmetic itself (product) are no longer exhibited.
アロエ茎葉に含まれる、このゲル質を構成する多糖体を
、通常、アロエセルロースとも呼び、分子量は、４５万
〜６５万にあるとも言われているが、一度、凝集、凝固
して、沈澱されたものは、水に不溶となり、その精製パ
ウダーは、現況では、化粧品分野に一部利用きれている
が、その他、健康食品向けのスリーム剤（肥満体防止剤
）や、ノンカロリー食として、顆粒や錠剤となして用い
られている。しかし、水に対する溶解性が低いために、
充分な利用は、進まないでいたのが現況であった。The polysaccharides that make up this gel substance contained in aloe stems and leaves are usually called aloe cellulose, and it is said to have a molecular weight of 450,000 to 650,000, but once it aggregates, coagulates, and precipitates. At present, the refined powder is partially used in the cosmetics field, but it is also used in granules as slimming agents (obesity prevention agents) for health foods and as non-caloric food. It is used as eggplant and tablets. However, due to its low solubility in water,
The current situation was that full utilization was not progressing.
ずなわち、アロエ茎葉中に含まれるアロエセルロースに
ついては、これが水に可溶な状態となすことが出来れば
、さらに広く利用が可能であったが、現況では、アロエ
茎葉のしぼり液の水溶１部を主体に用いられ、水に不溶
性のアロエセルロースについては、充分な利用がなされ
ないままであった。In other words, the aloe cellulose contained in aloe stems and leaves could be used more widely if it could be made soluble in water. Water-insoluble aloe cellulose, which is mainly used in water, has not been fully utilized.
〔口〕 発明の構成
本発明は、アロエ茎葉をもとに、分子量が１万以下の糖
類を得ることにある。[Mouth] Structure of the Invention The present invention is to obtain saccharides with a molecular weight of 10,000 or less based on aloe stems and leaves.
その具体的な手段については、次に示すごとくである。The specific means for doing so are as follows.
ｒ問題点の解決のための手段。rMeans for solving problems.
アロエ茎葉に含まれる、水に不溶性のアロエセルロース
について、完全な水溶性となす技術は、今だ公開されて
いない。As for the water-insoluble aloe cellulose contained in aloe stems and leaves, the technology to make it completely water-soluble has not yet been published.
本発明は、前項で述べた様に、アロエ茎葉のし＋？り液
にみられる、沈澱現象をとらえ、この沈澱物の有効利用
のために、水に可溶となすための手段について、種々の
検討を加えてきた。そして、アロエ茎葉のしぼり液から
沈澱物を除去し、その残った液中に含まれる、水に可溶
であるところのｍＭを分析してみると、液中に、熱にも
、低温下においても、安定的に沈澱を生じない糖類が確
認呼れ、それを分子量から求めるとき、１万以下にある
ことを見出すことが出来た。As mentioned in the previous section, the present invention is based on the aloe vera stems and leaves +? In order to capture the precipitation phenomenon observed in liquid solutions and to make effective use of this precipitate, various studies have been conducted on ways to make the precipitate soluble in water. After removing the precipitate from the squeezed liquid of aloe stems and leaves, we analyzed the water-soluble mM contained in the remaining liquid. In addition, we were able to identify a saccharide that does not form a stable precipitate, and when we calculated it from its molecular weight, we found that it was less than 10,000.
すなわち、アロエ茎葉中には、高分子な多糖類をはじめ
、低分子な糖類まで、種々含まれているが、これらの糖
類中、アロエ茎葉からのしぼり液において、沈澱を生じ
ない糖類となると、約０゜１〜０．３％であり、この部
分は、無色透明な液体として得られる。又、従来の多糖
体を主体とするアロエセルロースと呼ばれる乳白色状の
パウダーには、その１ｋｇ中からは、可溶性の多糖体、
及び低分子な可溶性のｍ類が、８〜１３．ｇａ度金言れ
ていることがわかった。ところが、これらの可溶性成分
は、経時的に沈澱が進み、次第に水に不溶となることで
ある。In other words, aloe leaves contain a variety of sugars, including high-molecular polysaccharides and low-molecular sugars, but among these sugars, there are sugars that do not precipitate in the squeezed liquid from aloe leaves. It is approximately 0.1 to 0.3%, and this portion is obtained as a colorless and transparent liquid. In addition, the conventional milky white powder called aloe cellulose, which is mainly composed of polysaccharides, contains soluble polysaccharides,
and low-molecular-weight soluble m-classes are 8 to 13. I found out that I can say a lot about money. However, these soluble components tend to precipitate over time and gradually become insoluble in water.
そこで、本発明者は、アロエ中の高分子多糖体であるア
ロエセルロースについて、種々の可溶化の検討を加えた
結果、以下に示すごとくの、加水分解による抽出法を採
用することにより、水に可溶な安定性のあるアロエセル
ロース由来の抽出物を得ることに成功した。Therefore, as a result of various studies on solubilization of aloe cellulose, which is a high-molecular polysaccharide in aloe, the present inventors found that it can be dissolved in water by adopting the extraction method by hydrolysis as shown below. We succeeded in obtaining a soluble and stable extract derived from aloe cellulose.
つまり、本発明の引き金となった技術手段は、アロエし
ぼり液中で、沈澱することなく、溶解が保持移れてきた
糖類に注目し、その分子量を求めるとき、１万以下の糖
類であることをたたき台となし、沈澱物の加水分解を試
み、低分子化することによって、従来のアロエセルロー
スと呼ばれる、水に不溶性の抽出物を、水に対して可溶
化させたことにある。In other words, the technical means that triggered the present invention focuses on saccharides that remain dissolved without precipitating in the aloe squeeze solution, and when determining their molecular weight, it is determined that the saccharides are 10,000 or less. As a starting point, we attempted to hydrolyze the precipitate and reduced the molecular weight, thereby making the conventional water-insoluble extract called aloe cellulose solubilized in water.
１実施例−１゜
（１）前処理工程
新鮮なアロエ茎葉１ｋｇを水洗いした後、その茎葉を、
高速回転式のミンチカッターにより、細断破砕し、圧搾
して得られた液を分取した後、Ｏ〜１５℃程度の冷暗所
に静１−’せ、上層水着液と、下層部沈澱液の２層に分
離し、上層水着液は、従来から公知な、アロイン等々を
含むエキス、又Ｉｔ、ＵＳＰ規格のアロエエキス（粉体
）のアロエ抽出物（水溶性）の粗原料用となす。1 Example - 1゜(1) Pretreatment step After washing 1 kg of fresh aloe stems and leaves with water, the stems and leaves were
After separating the obtained liquid by crushing it into pieces using a high-speed rotary mince cutter and squeezing it, it was placed in a cool, dark place at a temperature of about 0 to 15°C for 1-' to separate the upper layer swimsuit liquid and the lower layer precipitate liquid. It is separated into two layers, and the upper layer swimsuit liquid is used as a crude raw material for aloe extract (water-soluble), which is a conventionally known extract containing aloin, etc., or aloe extract (powder) according to the USP standard.
（２）加水分解工程
上述の工程で分離された下層部沈澱液を分取し、水分が
８０％〜６０％程度になるまで濃縮するか、そのままの
状態の液に、水酸化ナトリウムを加え、液中の水酸化ナ
トリウムの量が３０％程度の濃度になるまで加えた後、
約１゛〜２時間程、攪拌しながら加熱（８０℃前後）を
行なう。(2) Hydrolysis step The lower precipitate separated in the above step is collected and concentrated until the water content is about 80% to 60%, or sodium hydroxide is added to the solution as it is. After adding the amount of sodium hydroxide in the solution until it reaches a concentration of about 30%,
Heat (around 80°C) while stirring for about 1 to 2 hours.
（３）粗抽出物分取の工程
上述工程で加熱処理後は、−昼夜放置した後、塩酸で中
和を行ない、濾過を行なって、その濾液を取り出し、水
分が４０〜６０％程度になるまで濃縮した後、２〜３倍
量のエタノールを加えてから、よく攪拌して、−昼夜放
竺後、□沈澱物を分取する。この沈澱物は、本発明が目
的とするアロエ由来の糖類を主体とする、水に可溶な粗
抽出物である。(3) Step of crude extract fractionation After heat treatment in the above step, - After being left for day and night, neutralize with hydrochloric acid, filter, and take out the filtrate, which has a moisture content of about 40 to 60%. After concentrating to 100%, add 2 to 3 times the amount of ethanol, stir well, and after standing for a day and night, □ separate the precipitate. This precipitate is a water-soluble crude extract mainly composed of saccharides derived from aloe, which is the object of the present invention.
（４）精製工程
上述の工程において得られた粗抽出物は、今だこれを水
に溶解すると、若干の濁りを生じ、又、その溶解液は、
多少の色調を有し、清澄性が悪い場合が多い。(4) Purification process When the crude extract obtained in the above-mentioned process is dissolved in water, it becomes slightly cloudy, and the solution is
It has some color tone and often has poor clarity.
そこで、粗抽出物は、再び、水を加えて加熱する。加熱
の際には、少量の希硫酸を、粗抽出物に加えた後、水を
加えて加熱するか、アミラーゼを加えて、加水分解を行
なった後、加熱しても良いが、ここでのポイントは、ヨ
ウ素呈色試験によって、ヨウ素の呈色反応を示きなくな
るまで分解することが良い。Then, water is added to the crude extract and heated again. When heating, you can add a small amount of dilute sulfuric acid to the crude extract, then add water and heat, or add amylase to perform hydrolysis, and then heat. The important point is to decompose the iodine color test until it no longer shows any iodine color reaction.
もちろん、酸類を工程中で使用した際には、例えば、炭
酸カルシウムなどで中和し、それから濾過を行ない、得
られた濾液を取り、濃縮後は、再度、エタノールを用い
て、それによって得られた沈澱物を、濃縮乾燥させて、
本発明のアロエ由来の精製粉末となす。Of course, when acids are used in the process, they should be neutralized with, for example, calcium carbonate, then filtered, the resulting filtrate taken, and after concentration, ethanol may be used again. The precipitate was concentrated and dried,
Purified powder derived from aloe of the present invention.
収量は、前記（２）の工程中で分別した沈澱液１６Ａ、
／−ｍ４Ｊ、ＬＬＩＡＪｎ／ｅ？ｊｌＱＳ−ｎ４１１／
−Ｊ／ｌ＋−ｊ、ＭＮは、６〜８ｇ、上述の精製粉末は
、４〜７ｇ１ｊｌ後である。The yield is 16A of the precipitate separated in the step (2) above,
/-m4J, LLIAJn/e? jlQS-n411/
-J/l+-j, MN is 6 to 8 g, and the above purified powder is 4 to 7 g1jl.
ｒ実施例−２Ｊ
実施例−１で示される工程の（３）で得られた粗抽出物
、又は、実施例−１の工程の（４）で得られた精製粉末
をもとに、３〜６倍の水を加えて溶解後、セライトを加
えて濾過し、その得られた濾液を、アルミナ、陽イオン
交換樹脂、陰イオン交換樹脂、活性炭を用いて、順次通
した後、ペリフンカセットシステム（日本ミリボア・リ
ミテッド製）を用い、分子量が１万のペリコン膜を通し
て、溶出した溶液を得て、これを濃縮して粉末となすか
、適当な濃縮液の状態となし、本発明のアロエ由来の抽
出物を得る。r Example-2J Based on the crude extract obtained in step (3) of Example-1 or the purified powder obtained in step (4) of Example-1, After dissolving by adding 6 times the amount of water, celite is added and filtered, and the resulting filtrate is sequentially passed through alumina, cation exchange resin, anion exchange resin, and activated carbon. (manufactured by Nippon Millibore Ltd.) to obtain the eluted solution through a Pellicon membrane with a molecular weight of 10,000, and concentrate it into a powder or form an appropriate concentrated liquid. Obtain an extract of
収量は、粗抽出物からは、２５〜４８％程度が得られる
が、精製粉末からは、８０〜９５％の収率で得られる。The yield is approximately 25 to 48% from the crude extract, while the yield is from 80 to 95% from the purified powder.
〔ハ〕 発明の効果
上記の実施例−１又は２・で得られた、アロエ茎葉から
の分解抽出物は、水に対する溶解性が良く、経時的にも
沈澱物の発生が少ないこと、耐熱、耐酸性に優れ、冷水
にも可溶であり、又、エタノールを３０％含有する水と
の混液中にも、白濁を生ずることがないために、化粧料
、食品類への添加が容易であることが、本発明の優れた
効果として上げることが出来る。[C] Effects of the invention The decomposed extract from aloe stems and leaves obtained in Example 1 or 2 above has good solubility in water, little occurrence of precipitates over time, heat resistance, It has excellent acid resistance, is soluble in cold water, and does not become cloudy even when mixed with water containing 30% ethanol, making it easy to add to cosmetics and foods. This can be cited as an excellent effect of the present invention.
次表（第１表）は、前述した効果について、従来のアロ
エ茎葉からしぼったところの沈澱物をもとに、脱色等の
処理を加えて得られたところの白色粉末と、対比したと
ころの物性を示したものであるが、その優れた溶解性は
、分子量から、１万以下の糖類を分取することによって
達成出来たものと言える。The following table (Table 1) shows the above-mentioned effects compared to a white powder obtained by applying treatments such as decolorization based on the precipitate squeezed from conventional aloe stems and leaves. Although the physical properties are shown, it can be said that the excellent solubility was achieved by fractionating saccharides with a molecular weight of 10,000 or less.
尚、末法による抽出物を構成する糖類についてみると、
ブドウ糖は少なく、全体を１００とするとき、ブドウ糖
は２〜３％、マルトースが４〜７％程度、トリオースが
８〜１５％、その他、テトラオース、ペンタオース、ヘ
キサオース、ペンタオース及び、ベプタオース以上の糖
類で、６８〜８２％を占めており、分子量からすれば、
１万以下の糖類で構成きれている。 ・
つまり、第１表で示すごとくの優れた溶解性は、従来の
アロエセルロースを主体となす抽出物では、５〜７万に
、その分子量のピークがあり、本発明によるｍ＊の構成
比率とは大きな違（１カ（ある。Regarding the sugars that make up the extract obtained by the powder method,
Glucose is small, and when the total is 100, glucose is 2 to 3%, maltose is about 4 to 7%, triose is 8 to 15%, and other sugars such as tetraose, pentaose, hexaose, pentaose, and veptaose are It accounts for 68-82%, and considering the molecular weight,
It is made up of less than 10,000 sugars.・In other words, the excellent solubility as shown in Table 1 is due to the molecular weight peak of 50,000 to 70,000 in the conventional extract mainly composed of aloe cellulose, and the m* composition ratio according to the present invention. There is a big difference (1ka).
つまり、本発明によるアロエ抽出物は、アロエ中の高分
子な多糖体をもとに、それを低分子化させることにより
、水に可溶化させたこと番こよって、化粧品や健康食品
に用いられやすくしたことにある。又、ブドウ糖含量が
少なく、〕、〕ンカロリー食にも有利の条件を有してい
る。In other words, the aloe extract according to the present invention is based on the high-molecular polysaccharides in aloe, and is made solubilized in water by reducing the molecular weight thereof. The reason is that we made it easier. In addition, it has a low glucose content, making it advantageous for low-calorie diets.
Claims (1)
を特徴とする、アロエ由来水溶性抽出物。(1) A water-soluble extract derived from aloe, characterized in that its main component is saccharide with a molecular weight of 10,000 or less.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61107505A JPS62263193A (en) | 1986-05-09 | 1986-05-09 | Water-soluble extract derived from aloe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61107505A JPS62263193A (en) | 1986-05-09 | 1986-05-09 | Water-soluble extract derived from aloe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62263193A true JPS62263193A (en) | 1987-11-16 |
| JPH0253441B2 JPH0253441B2 (en) | 1990-11-16 |
Family
ID=14460908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61107505A Granted JPS62263193A (en) | 1986-05-09 | 1986-05-09 | Water-soluble extract derived from aloe |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62263193A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001520019A (en) * | 1997-10-10 | 2001-10-30 | ユニバーラ ファーマスーティカルズ，インコーポレイテッド | Production of immunomodulatory polysaccharides from aloe |
| KR20030088201A (en) * | 2002-05-13 | 2003-11-19 | (주)제노덤 | Compositions for aloe vera mask solution and processing make the same |
| US10449143B2 (en) | 2014-04-17 | 2019-10-22 | Conopco, Inc. | Aloe vera extract for personal care compositions |
| US10456353B2 (en) | 2014-04-17 | 2019-10-29 | Conopco, Inc. | Personal care compositions |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49132211A (en) * | 1973-04-21 | 1974-12-18 |
-
1986
- 1986-05-09 JP JP61107505A patent/JPS62263193A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49132211A (en) * | 1973-04-21 | 1974-12-18 |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001520019A (en) * | 1997-10-10 | 2001-10-30 | ユニバーラ ファーマスーティカルズ，インコーポレイテッド | Production of immunomodulatory polysaccharides from aloe |
| EP1036187A4 (en) * | 1997-10-10 | 2002-03-27 | Univera Pharm Inc | Process for the preparation of immunomodulatory polysaccharides from aloe |
| US6436679B1 (en) | 1997-10-10 | 2002-08-20 | Unigen Pharmaceuticals, Inc. | Process for the preparation of immunodulatory polysaccharides from aloe |
| KR20030088201A (en) * | 2002-05-13 | 2003-11-19 | (주)제노덤 | Compositions for aloe vera mask solution and processing make the same |
| US10449143B2 (en) | 2014-04-17 | 2019-10-22 | Conopco, Inc. | Aloe vera extract for personal care compositions |
| US10456353B2 (en) | 2014-04-17 | 2019-10-29 | Conopco, Inc. | Personal care compositions |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0253441B2 (en) | 1990-11-16 |
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