JPS62184345A - Method for measuring ion concentration - Google Patents
Method for measuring ion concentrationInfo
- Publication number
- JPS62184345A JPS62184345A JP61025962A JP2596286A JPS62184345A JP S62184345 A JPS62184345 A JP S62184345A JP 61025962 A JP61025962 A JP 61025962A JP 2596286 A JP2596286 A JP 2596286A JP S62184345 A JPS62184345 A JP S62184345A
- Authority
- JP
- Japan
- Prior art keywords
- ion
- measured
- measuring
- measurement
- sensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 8
- 238000005259 measurement Methods 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 239000000126 substance Substances 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 abstract 7
- 230000010354 integration Effects 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 description 37
- 239000003085 diluting agent Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- -1 FET ion Chemical class 0.000 description 4
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 4
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 description 2
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229910001414 potassium ion Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- GTKRFUAGOKINCA-UHFFFAOYSA-M chlorosilver;silver Chemical compound [Ag].[Ag]Cl GTKRFUAGOKINCA-UHFFFAOYSA-M 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000011505 plaster Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
Description
【発明の詳細な説明】
〔発明の技術分野〕
本発明は測定試料液中のとくに、血液など体液中のイオ
ン濃度の測定を行なうＦＥＴイオンセンサ（以下、ｌ５
ＦＨＴともいう）を用いたイオンを一度の測定方法に関
する。Detailed Description of the Invention [Technical Field of the Invention] The present invention relates to an FET ion sensor (hereinafter referred to as 15
It relates to a method for measuring ions once using FHT (also referred to as FHT).
従来より、成牛の特定のイオン一度を選択的に定量でき
るイオン選択性電極が、特定イオンのモニターや水質分
ケ「寺の広い分野で用いられている。Ion-selective electrodes that can selectively quantify specific ions in adult cows have been used in a wide range of fields, including monitoring specific ions and analyzing water quality.
近年では、特に血液中のＮａ、に、Ｃｔ−等の各種イオ
ンの定量などがイオン選択性電極を用いてさかん屹行な
われている。In recent years, ion-selective electrodes have been increasingly used to quantify various ions, particularly Na, Ct-, and the like in blood.
このようなイオン選択性電極の中で、従来のイオン選択
性電極に代わって、絶縁ゲート型のＦＥ’ｌ’のゲート
部にイオン感応膜を形成したＦＥＴイオンセンサが注目
されるようになってきた。これは、ｉ’ｇ’ｒイオンセ
ンサが、トランジスタやＩＣ，ＬＳＩなどをつくるとき
に用いるシリコンプロセスを用いることにより同時に複
数個つくることができ、多重化が容易で、しかも小型化
が容易であることとや、大量生産が可能なため低コスト
化が期待できるなどの理由による。このようなＦｋＴイ
オンセンサは、小型化、多重化に適することから、複数
の測定項目を対象としたマルチタイプのＦＥＴイオンセ
ンサ（以下、マルチｌ５ＦＥＴともいう）が種々考案さ
れている。Among such ion-selective electrodes, FET ion sensors, in which an ion-sensitive membrane is formed on the gate of an insulated gate type FE'l', have been attracting attention instead of conventional ion-selective electrodes. Ta. This is because multiple i'g'r ion sensors can be manufactured at the same time by using the silicon process used to make transistors, ICs, LSIs, etc., making it easy to multiplex and downsize. This is because it can be mass-produced and can be expected to reduce costs. Since such FkT ion sensors are suitable for miniaturization and multiplexing, various multi-type FET ion sensors (hereinafter also referred to as multi-I5FETs) that target a plurality of measurement items have been devised.
ところでこのようなに’ｎＴイオンセンサにおいても、
通常のイオンセンサと同様にイオンＲ＆の測定の際には
基準電極を用いて、基準電極と測定電極の間の電位差を
求める必要がある。従来より、こうした基準電極として
は、たとえば飽和せコウ電極や銀−塩化銀電極などが用
いられてきている。By the way, even in such a 'nT ion sensor,
As with a normal ion sensor, when measuring ions R&, it is necessary to use a reference electrode and determine the potential difference between the reference electrode and the measurement electrode. Conventionally, as such a reference electrode, for example, a saturated plaster electrode, a silver-silver chloride electrode, etc. have been used.
このような基準１！極を安定状態で使うためには、この
基Ｍ［極を構成する内部電極に接して基準電位を発生さ
せるための電解質溶液が必要であり、その電解質溶液を
保持するため、上記の如き基準電極はＦｋ、Ｔイオンセ
ンサの形状に比べて非常に大型にならざるを得なかった
。したがって、これらの基準１Ｊｃ＃Ａをマルチｌ５Ｆ
ＥＴ用として組み込むことは小型化を目指している複数
のマルチタイプを組込だマルチｌ８ＦＥＴの特長が活か
せなくなり、問題であった。Standard 1 like this! In order to use the electrode in a stable state, an electrolyte solution is required to generate a reference potential in contact with the internal electrodes that make up the electrode. had to be much larger than the Fk and T ion sensors. Therefore, these standards 1Jc#A are multi-l5F
Incorporating it for ET was a problem because the features of the multi-l8FET, which incorporates multiple multi-types aimed at miniaturization, could not be utilized.
たとえ仮に、このような基本構成を有する上記基準電極
を小型化してマルチｌ５ＦＥＴ中に組み込んだとしても
、その内部電極に接する電解液量が少くならざるを得な
いため、基準ｔｔ極として安定性に欠は短寿命にならざ
るを得ないという欠点があった〇
一方これに対して、絶縁ゲート型電界効果トランジスタ
のゲート部にイオン非感応性の膜を形成した小型基準電
極が考えられ、種々のテストが繰り返されている。しか
しながらこれまで長寿命なイオン非感応性膜を形成した
基準電極は開発されていない。Even if the above-mentioned reference electrode having such a basic configuration were miniaturized and incorporated into a multi-l5FET, the amount of electrolyte in contact with the internal electrode would inevitably be small, resulting in poor stability as a reference TT electrode. On the other hand, a small reference electrode with an ion-insensitive film formed on the gate of an insulated gate field effect transistor has been considered, and various tests have been repeated. However, no reference electrode with a long-life ion-insensitive membrane has been developed so far.
本発明は上記欠点に対してなされたものであり、安定に
測定物質の検出ができるイオン濃度の測定方法を提供す
ることを目的とする。The present invention has been made to solve the above-mentioned drawbacks, and an object of the present invention is to provide a method for measuring ion concentration that can stably detect a substance to be measured.
本発明は、絶縁ゲート型トランジスタのゲート領域に、
測定試料液中の被測定物質以外の物質を測定対象とする
イオン選択膜を形成してなるＰＥＴイオンセンサを、前
記測定液中の被測定物を測定する際の基準電極として用
いることを特徴とする。In the present invention, in the gate region of an insulated gate transistor,
A PET ion sensor formed with an ion-selective membrane that targets substances other than the substance to be measured in the measurement sample liquid is used as a reference electrode when measuring the substance to be measured in the measurement liquid. do.
このような本発明に係るイオン濃度の測定方法では、測
定に用いるマルチｌ８Ｆｊ（Ｔにおいて被測定物質以外
のそのイオン誼度変動が無視できる物質を測定対象とす
るＦＥＴセンサを基準電極として用いるので、測定用の
ｌ８ＦＥＴとの１体化、小型化が可能である。さらには
従来のｌ８ＦＥＴではない構成のイオン選択性電極を基
準電極として用いた場合では、イオン選択膜の抵抗が大
きく測定中にノイズを拾いやすい等の問題点があるが、
ＦｆｆＴセンサを基準電極として用いた場合には、セン
サ自体がインピーダンス変換器の働きを有しているため
、ノイズの影響をほとんど受けずに測定できる。In the method for measuring ion concentration according to the present invention, a multi-I8Fj (T) FET sensor whose measurement target is a substance whose ion density fluctuation can be ignored other than the substance to be measured is used as a reference electrode. It is possible to integrate it with an 18FET for measurement and make it smaller.Furthermore, when an ion-selective electrode with a configuration other than a conventional 18FET is used as a reference electrode, the resistance of the ion-selective membrane is large, causing noise during measurement. There are some problems, such as the fact that it is easy to pick up
When an FffT sensor is used as a reference electrode, since the sensor itself functions as an impedance converter, measurement can be performed with almost no influence from noise.
本発明に係るイオン濃度の測定方法はイオンセンサとし
て小型化が可能なＩ”ＥＴセンサの特長を損なわずに、
かつ、安定に被測定物質を測定でき、工業的価値は大で
ある。The method for measuring ion concentration according to the present invention does not impair the features of the I"ET sensor, which can be miniaturized as an ion sensor.
In addition, the substance to be measured can be measured stably, and it has great industrial value.
以下、本発明をさらに実施例にもとづき詳しく説明する
。Hereinafter, the present invention will be further explained in detail based on Examples.
第１図の模式図は本発明の一実施例としての流通製マル
チＩＳＦｇＴ及びこのマルチＩＳＦ’ｎＴの駆動部、デ
ータ処理部等を含む測定糸を示す。図中点！ （１）内
は流通型マルチｌ５ｌＩ’ＥＴを模式的に示したもので
あり、（２）は試料の流通路である。（３）は流通路壁
で、（４）、（４ｇ ）、（４ｇ ）はそれぞれ絶縁ゲ
ー） ｆｆ ＦＥＴの表面にイオン感応膜を形成したイ
オン選択性電極であり、（４１）はナトリウムイオンセ
ンサ、（４Ｓ）はカリウムイオンセンサ（４３）は硝酸
イオンセンサである。ここで、硝酸イオンセンサ（４３
）は基準電極として用いるイオン選択性Ｆ］！ｉＴセン
サである。（５）は各々のｌ８ＦＥＴのソース部に接続
されている白金電極であり、測定液との間にアースをと
るためのものである。（６）は前記の各々のｌ５ＦＥＴ
に、ドレンとソースからのリード線によって接続されて
いる前記の各々のｌ８ＦＥＴへの加電圧回路である。さ
らに、（７）は前記各々のＩ Ｓ Ｆ）、：Ｔにおける
ドレン電流値からイオン湿度を算出するためのデータ処
理部、（８）は表示部である。なお、（９，）、（９宜
）、（９ｍ）はそれぞれのＦＥＴの絶縁ゲート部に形成
されたイオン感応部である。本測定系に８いて、試料の
流通路（２）にたとえば血精をＣａ（ＮＯ，）２を数ｌ
ｏｍＭ含有する希釈液で希釈したサンプルを通すことζ
こより、一定量であるＮＯ；イオン縫度を基準に被測定
物資であるＮａ、Ｋ イオン濃度を測定することができ
る。この時、ｆｉｌｌｌ定に先立って標準液により較正
を行なっておく。血液中にはＮＯ；はほとんど存在しな
いのでＮ０７を含む希釈液で測定することにより硝酸イ
オンセンサからの出力を基準電位しして得ることができ
る。なお、血液中ではり、、Ｃａ も微量にしか存在
しないため、Ｌ１イオン選択性ＦＥＴセンサ、Ｃａ
イオン選択性Ｆｌｊ’ｒセンサを基準電極として含むマ
ルチタイプのＦＥＴイオンセンサを用いて血中イオン濃
度の分析も可能である。The schematic diagram in FIG. 1 shows a multi-ISFgT produced by a distribution company as an embodiment of the present invention, and a measuring thread including a driving section, a data processing section, etc. of this multi-ISF'nT. The middle point of the diagram! (1) is a schematic diagram of a flow-through type multi-l5lI'ET, and (2) is a sample flow path. (3) is the flow channel wall, (4), (4g), and (4g) are ion-selective electrodes with an ion-sensitive membrane formed on the surface of an insulating gate (FFFET), and (41) is a sodium ion sensor. , (4S), the potassium ion sensor (43) is a nitrate ion sensor. Here, the nitrate ion sensor (43
) is the ion selectivity F used as a reference electrode! It is an iT sensor. (5) is a platinum electrode connected to the source part of each 18FET, and is used to establish a ground between the electrode and the measurement liquid. (6) is each of the above 15FETs
and a voltage applying circuit to each of the above-mentioned 18FETs connected by lead wires from the drain and source. Furthermore, (7) is a data processing unit for calculating the ion humidity from the drain current value at each of the above-mentioned ISF), :T, and (8) is a display unit. Note that (9,), (9y), and (9m) are ion sensitive parts formed in the insulated gate part of each FET. In this measuring system, for example, several liters of blood semen and Ca(NO,)2 are added to the sample flow path (2).
Passing the sample diluted with a diluent containing omMζ
From this, it is possible to measure the concentration of Na and K ions, which are the substances to be measured, based on the constant amount of NO; ion density. At this time, calibration is performed using a standard solution prior to fill determination. Since there is almost no NO; in blood, by measuring with a diluent containing N07, the output from the nitrate ion sensor can be obtained as a reference potential. In addition, since Ca exists only in trace amounts in blood, L1 ion selective FET sensor, Ca
Blood ion concentration analysis is also possible using a multi-type FET ion sensor that includes an ion-selective Flj'r sensor as a reference electrode.
第２図に第１図で示したマルチＩＳ１！’ｌ’を用いた
測定液、希釈液等の流れを示した。αυは基準電極とし
てのイオン選択性ＦＥＴセンサを組み込んだマルチｌ８
ＦＥＴである。圓は希釈液の容器、０３は測定対象とし
ての血清である。（１４１はポンプであり前記血清ｕｎ
ｉ’や希釈液α４′を試料調製容器α四に導く。Figure 2 shows the multi-IS1 shown in Figure 1! The flow of measurement solution, dilution solution, etc. using 'l' is shown. αυ is a multi-l8 incorporating an ion-selective FET sensor as a reference electrode.
It is an FET. Circle is a container for a diluent, and 03 is serum as a measurement target. (141 is a pump and the serum un
i' and the diluent α4' are introduced into the sample preparation container α4.
ｑｌｊは切換えコックである。ポンプ（１７＞は前記マ
ルチｌ５ＦＥＴαυに希釈欣により所定のｚ軸度に希釈
された測定試料敢を導く働きをする。（１８１は廃液タ
ンクである。なお、翰は前記ポンプα力、Ｑη、コック
αｅを駆動させるための駆動装置である。このようなマ
ルチタイプのＦｇ’ｒセンサーに基準電極としてのに″
ＥＴイオンセンサを組み込んでなる分析装置では、従来
の基準電極を用いた場合と比べて小型になり、ノイズ等
に強く安定した測定ができる。qlj is a switching cock. The pump (17) serves to guide the measurement sample diluted to a predetermined z-axis degree by the dilution pump to the multi-l5FET αυ. (181 is a waste liquid tank. The handles are the pump α force, Qη, and the cock. This is a driving device for driving αe.
An analyzer incorporating an ET ion sensor is smaller than that using a conventional reference electrode, is resistant to noise, and can perform stable measurements.
第１図は本発明の一実施例としての（）ｔｌｌｌｌ決方
法現するためのイオン濃度の測定系を示す模式図、第２
図はマルチＩｆ：１ＦＥＴを中心にした測定歇、希釈液
等の流れを示した模式図である。
（Ｉ）、αυ・・・流通型マルチＩｎｋ″ＪＩＴ、（２
）・・・流通路、（４）・・・ナトリウムイオンセンサ
、（４）・・・カリウムイオンセンサ、（４ｍ）・・・
硝酸イオンセンサ、ｔｌＪ’・・・希釈液、ｕ３１’・
・・測定液、ｕｌ・・・駆動装置。
代理人 弁理士 則 近 憲 佑
同 竹 花 番久男FIG. 1 is a schematic diagram showing an ion concentration measurement system for realizing the ()tllll determination method as an embodiment of the present invention, and FIG.
The figure is a schematic diagram showing the flow of measurement intervals, diluent, etc. centering around the multi-If:1 FET. (I), αυ...Distribution type multi-Ink''JIT, (2
)...Flow path, (4)...Sodium ion sensor, (4)...Potassium ion sensor, (4m)...
Nitrate ion sensor, tlJ'... diluent, u31'.
...Measurement liquid, ul...Drive device. Agent Patent Attorney Nori Chika Yudo Takehana Bank Hisao
Claims (1)
中の被測定物質以外の物質を測定対象とするイオン選択
膜を形成してなるＦＥＴイオンセンサを、前記測定液中
の被測定物質を測定する際の基準電極として用いること
を特徴とするイオン濃度の測定方法。When measuring a substance to be measured in the measurement liquid, an FET ion sensor is formed by forming an ion-selective membrane on the gate region of an insulated gate transistor to measure substances other than the substance to be measured in the measurement sample liquid. A method for measuring ion concentration, characterized in that it is used as a reference electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61025962A JPS62184345A (en) | 1986-02-10 | 1986-02-10 | Method for measuring ion concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61025962A JPS62184345A (en) | 1986-02-10 | 1986-02-10 | Method for measuring ion concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62184345A true JPS62184345A (en) | 1987-08-12 |
Family
ID=12180364
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61025962A Pending JPS62184345A (en) | 1986-02-10 | 1986-02-10 | Method for measuring ion concentration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62184345A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6457161A (en) * | 1987-08-28 | 1989-03-03 | Shindengen Electric Mfg | Electrochemical measuring method |
| JPS6466558A (en) * | 1987-09-07 | 1989-03-13 | Toa Electronics | Oxidizing/reducing potential difference |
| US8502277B2 (en) | 2003-08-29 | 2013-08-06 | Japan Science And Technology Agency | Field-effect transistor, single-electron transistor and sensor using the same |
-
1986
- 1986-02-10 JP JP61025962A patent/JPS62184345A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6457161A (en) * | 1987-08-28 | 1989-03-03 | Shindengen Electric Mfg | Electrochemical measuring method |
| JPS6466558A (en) * | 1987-09-07 | 1989-03-13 | Toa Electronics | Oxidizing/reducing potential difference |
| US8502277B2 (en) | 2003-08-29 | 2013-08-06 | Japan Science And Technology Agency | Field-effect transistor, single-electron transistor and sensor using the same |
| US8766326B2 (en) | 2003-08-29 | 2014-07-01 | Japan Science And Technology Agency | Field-effect transistor, single-electron transistor and sensor |
| US8772099B2 (en) | 2003-08-29 | 2014-07-08 | Japan Science And Technology Agency | Method of use of a field-effect transistor, single-electron transistor and sensor |
| US9506892B2 (en) | 2003-08-29 | 2016-11-29 | Japan Science And Technology Agency | Field-effect transistor, single-electron transistor and sensor using the same |
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