JPS62177446A - Instrument for measuring ionic activity - Google Patents

Instrument for measuring ionic activity

Info

Publication number
JPS62177446A
JPS62177446A JP61019950A JP1995086A JPS62177446A JP S62177446 A JPS62177446 A JP S62177446A JP 61019950 A JP61019950 A JP 61019950A JP 1995086 A JP1995086 A JP 1995086A JP S62177446 A JPS62177446 A JP S62177446A
Authority
JP
Japan
Prior art keywords
liquid
ion
electrodes
porous
distribution member
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP61019950A
Other languages
Japanese (ja)
Other versions
JPH0556819B2 (en
Inventor
Osamu Seshimoto
修 瀬志本
Masaaki Terajima
正明 寺嶋
Yoshio Saito
斉藤 義雄
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Holdings Corp
Original Assignee
Fuji Photo Film Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Photo Film Co Ltd filed Critical Fuji Photo Film Co Ltd
Priority to JP61019950A priority Critical patent/JPS62177446A/en
Priority to US07/009,470 priority patent/US4842712A/en
Priority to DE8787101366T priority patent/DE3766835D1/en
Priority to EP87101366A priority patent/EP0231033B1/en
Publication of JPS62177446A publication Critical patent/JPS62177446A/en
Publication of JPH0556819B2 publication Critical patent/JPH0556819B2/ja
Granted legal-status Critical Current

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Abstract

PURPOSE:To measure a blood sample without errors by supplying each of the liquid to be inspected and reference liq. to the 1st electrodes and 2nd electrodes, providing porous liquid distributing members to the respective electrodes and using non-woven cellulose fabrics as the distributing members. CONSTITUTION:The liquid to be inspected and reference liq. are supplied to each of spotting holes 36 of an upper cap 35 and are stored in a liquid storage tank 34. A fiber bridge 38 is provided between the two spotting holes 36. The porous liquid distributing members 32 are provided under the tanks 34 and the respective liquids are passed through the members 32 so as to flow into the holes 31 of a water impermeable sheet member 30. The liquid to be inspected and reference liq. are passed to a sheet-shaped solid electrode pair 26 having an ion selection layer atop the same. Connecting terminals 27 are provided to the electrodes 26 and a potential difference between the ion selective electrodes 26 is detected to measure the ionic activity. The non-woven fabrics consisting of long fiber cellulose are used as the porous liquid distributing members 32. Since the non-woven fabrics are used for the distributing members 32, the measurement error arising from the hemolysis generated in the stage of measuring the potassium ion activity in blood is eliminated.

Description

【発明の詳細な説明】 [発明の技147分野] 本発明は、水性液体、特に生物体液(血液、尿、唾液等
)中の特定のイ・オンの活量(または濃度)をポテンシ
ョメトリーを利用して定量分析するためのイオン活量測
定器具に関する。
Detailed Description of the Invention [147 Fields of the Invention] The present invention relates to potentiometrically measuring the activity (or concentration) of specific ions in aqueous fluids, particularly biological fluids (blood, urine, saliva, etc.). This invention relates to an ion activity measuring instrument for quantitative analysis.

[発明の背景] 液体(水道水、河川水、下水、産業排水など)や生物体
液(血液、尿、唾液等)の液滴量を用い、その中に含ま
れる特定のイオンの活量をシート状のイオン活量測定器
具を用いてUl定する方法はすでに知られている。
[Background of the invention] Using the droplet volume of liquids (tap water, river water, sewage, industrial wastewater, etc.) and biological fluids (blood, urine, saliva, etc.), the activity of specific ions contained therein is calculated as a sheet. A method for determining Ul using an ion activity measuring instrument is already known.

すなわち、互いに電気的に分離された一対のイオン選択
電極のそれぞれのイオンj1択層表面に参照液および被
検液を付与し、ついでブリッジにより両液体を互いに電
気的に導通させた状態において、各イオン還択電極間の
電位差を測定して、被検液体のイオン活量を測定する方
法である。そのようなイオン活量測定器具の例としては
、特開昭52−142586号、特開昭56−6148
号、特開昭58−211048号、等に記載されている
イオン活量測定器具をあげることができる。
That is, a reference liquid and a test liquid are applied to the surface of each ion selection layer of a pair of ion-selective electrodes that are electrically separated from each other, and then both liquids are electrically connected to each other by a bridge. This method measures the potential difference between ion reduction electrodes to measure the ionic activity of the test liquid. Examples of such ion activity measuring instruments include JP-A-52-142586 and JP-A-56-6148.
For example, the ion activity measuring instrument described in Japanese Patent Application Laid-open No. 58-211048 and the like can be mentioned.

これらのイオン活量測定器具は、基本的には1対のシー
ト状イオン選択電極をイオン選択電極が上側になるよう
に配置し、その上に液点着孔(標準液と被検液のf1与
を行うための開口部)を設けており、上方からそれぞれ
のイオン3’A択層上に、液点着孔を介しピペットなど
を用いて参照液および被検液を付与し、両イオン選択電
極間に発生する電位差を測定することにより、イオン活
量を測定する。一方、1個のイオン活量測定器具に複数
組のイオンJ2u択電極対をmみ込んで、参照液と被検
液とをそれぞれ1回付与することによりtM数種類のイ
オンの活量を測定出来るようにしたものが特開昭58−
211648号で知られている。
These ion activity measuring instruments basically have a pair of sheet-like ion-selective electrodes arranged with the ion-selective electrode facing upward, and a liquid spotting hole (f1 for standard solution and test solution) The reference solution and test solution are applied from above onto each ion 3'A selection layer using a pipette or the like through the liquid spotting hole, and both ions are selected. Ion activity is measured by measuring the potential difference generated between the electrodes. On the other hand, the activities of several types of ions can be measured by incorporating multiple sets of ion J2u selective electrode pairs into one ion activity measurement device and applying the reference solution and test solution once each. This was published in Japanese Unexamined Patent Application Publication No. 58-
It is known as No. 211648.

上記のような複数のシーI・状イオン選択電極対を用い
て腹数種のイオン活量を実質上同時に測定する方法は簡
便で優れた方法であるが、下記の問題があることが判明
した。
Although the above-mentioned method of measuring the ion activities of several species at the same time using multiple C-shaped ion-selective electrode pairs is a simple and excellent method, it has been found that there are the following problems. .

すなわち多孔性液体分配部材として局方包帯、麻かや地
、寒冷じゃ、絽、ろ紙などを用いた場き、全血、全血希
釈液、またはそれに準する、血球(特に赤血球)を含む
血液試料中のイオン活量、特にカリウムイオン活量をj
、pl定するとき、血液試料がイオン活量測定器具内に
おいて溶血をおこし、そのためにカリウムイオン活量の
測定値が血液試料中の真のカリウムイオン活量と異なる
ことがしばしば生じた。これは同じ血液試料から血球を
除去して得た血漿又は血清で測定したカリウノ、イオン
活量との不一致として検出された。
In other words, when a topical bandage, linen fabric, cold cloth, rug, filter paper, etc. are used as a porous liquid distribution member, whole blood, diluted whole blood, or similar blood containing blood cells (especially red blood cells) can be used. The ion activity in the sample, especially the potassium ion activity, is
, pl, the blood sample undergoes hemolysis within the ion activity measuring instrument, which often results in the measured value of potassium ion activity being different from the true potassium ion activity in the blood sample. This was detected as a discrepancy with the ion activity measured in plasma or serum obtained by removing blood cells from the same blood sample.

一方、多孔性液体分配部材として局方ガーゼを用いれば
全血の溶血は比較的少ないが、変形しやすく、一定の寸
法に切断することが非常に難しい。
On the other hand, if pharmacopoeial gauze is used as a porous liquid distribution member, hemolysis of whole blood is relatively small, but it is easily deformed and is very difficult to cut to a certain size.

合成ポリマー繊tしたとえばポリエステル繊維の布も全
血の溶血を余り生じないが、液の展開が遅い。
Synthetic polymer fabrics, such as polyester fabrics, cause less hemolysis of whole blood, but the liquid spreads slowly.

これらの材料はそれ故、複数種のイオン活量を一個の器
具を用いて実質的に同時に測定するための前記のような
イオン活量測定器具の液体分配部材として用いるのに適
しない。
These materials are therefore not suitable for use as liquid distribution members in ion activity measurement devices such as those described above for measuring the ion activities of multiple species substantially simultaneously using a single device.

[発明の目的コ 本発明の目的は、全血など血球を含む血液試料中のカリ
ウムイオン活量測定において見られる上記のような溶血
にもとすく測定誤差を除去したイオン活量測定器具の提
供にある。
[Purpose of the Invention] An object of the present invention is to provide an ion activity measuring instrument that easily eliminates measurement errors due to the above-mentioned hemolysis that occurs when measuring potassium ion activity in blood samples containing blood cells such as whole blood. It is in.

本発明の池の目的は、全血など血球を含む血液試料中の
複数種のイオン活量を一個の器具を用いて実質的に同時
に測定するに有用なイオン活量測定器具であって、カリ
ウムイオン活量pJll定の際の溶血にもとすく測定誤
差を除去したイオン活量測定器具の提供にある。
The object of the present invention is to provide an ion activity measuring device useful for substantially simultaneously measuring the ion activities of multiple species in a blood sample containing blood cells such as whole blood using one device, An object of the present invention is to provide an ion activity measuring instrument that quickly eliminates measurement errors due to hemolysis when determining the ion activity pJll.

本発明の上記目的の−は、特定イオンに選択的に応答す
るイオン選択電極の対と、前記電極の対のうち第一の電
極に被検液を供給できる多孔性液体分配部材と、前記電
極の対のうち第二の電極に参照液を供給できる多孔性液
体分配部材と、各電極に供給される被検液と参照液との
間に電気的導通を達成するための多孔性ブリッジをそな
えるイオン活量測定器具であって、前記液体分配部材が
長繊維セルロースよりなる不織布であることを特徴とす
るイオン活量測定器具によって達成された。
The object of the present invention is to provide a pair of ion-selective electrodes that selectively respond to specific ions, a porous liquid distribution member capable of supplying a test liquid to a first electrode of the pair of electrodes, and a porous liquid distribution member that can supply a test liquid to a first electrode of the pair of electrodes. a porous liquid distribution member capable of supplying a reference liquid to the second electrode of the pair, and a porous bridge for achieving electrical continuity between the test liquid and the reference liquid supplied to each electrode. This has been achieved by an ion activity measuring device characterized in that the liquid distribution member is a nonwoven fabric made of long fiber cellulose.

本発明の上記目的の他の一つは、特定イオンに選択的に
応答するイオン選択電極の対と、前記電極の対のうち第
一の電極に被検液を供給できる多孔性液体分配゛部材と
、前記電極の対のうち第二の電極に参照液を供給できる
多孔性液体分配部材と、各電極に併給される被検液と9
照液との間に電気的導通を達成するための多孔性ブリッ
ジをそなえるイオン活量測定器具であって、前記イオン
選択電極を少なくとも2対そなえ、前記液体分配部材が
長繊維セルロースよりなる不織布であることを特徴とす
るイオン活量測定器具によって達成された。
Another object of the present invention is to provide a pair of ion-selective electrodes that selectively respond to specific ions, and a porous liquid distribution member capable of supplying a test liquid to the first electrode of the pair of electrodes. , a porous liquid distribution member capable of supplying a reference liquid to the second electrode of the pair of electrodes, and a test liquid supplied to each electrode together;
An ion activity measuring device comprising a porous bridge for achieving electrical continuity with a liquid, the device comprising at least two pairs of the ion-selective electrodes, and the liquid distribution member being made of a nonwoven fabric made of long-fiber cellulose. This was achieved by an ion activity measuring instrument having the following characteristics.

長繊維セルロースより成る不織布としては、バインダー
を実質的に含まないセルロース系スパンボンド不繊布が
好ましく、特にコツトンリンターを原料とする長繊維か
らなるものが好ましい、たとえば、コツトンリンターを
シュバイツア液に溶解してなる紡糸液からスパンボンド
法で製造した不織布が好ましい、このような不織布は、
一般に、3秒間に部材の重量の少なくとも10倍の血液
を吸収することができる。
The nonwoven fabric made of long-fiber cellulose is preferably a cellulose-based spunbond nonwoven fabric that does not substantially contain a binder, and is particularly preferably made of long fibers made from cotton linters. Preferably, the nonwoven fabric is produced by a spunbond method from a dissolved spinning solution.
Generally, at least 10 times the weight of the member can be absorbed in 3 seconds.

本発明で用いる多孔性液体分配部材の厚さは約100μ
ないし400μ程度が適当である。
The thickness of the porous liquid distribution member used in the present invention is approximately 100μ.
Approximately 400μ to 400μ is appropriate.

本発明で用いる多孔性液体分配部材は、特願昭59−2
44200号に記載されたような形状とすることもでき
る。
The porous liquid distribution member used in the present invention is disclosed in Japanese Patent Application No. 59-2
It can also have a shape as described in No. 44200.

本発明のイオン活量測定器具は種々の具体的態様をとる
ことができるが、たとえば特開昭58−211648号
に記載されたj:4造とすることができる。たとえば、
第1図に示すように、イオン選択層を上面に有するシー
ト状固体電(か対26、電気接続端子部27、複数の固
体型(斬対を収納する支持枠28、固体電極対の表面を
覆う水工3S性シート部材30、水不透性部材30に設
けられた液供給孔31、液gt給孔31に液を分配する
多孔性部材32、被検液および参照液をそれぞれ受容す
る2つの貯液Pa34、点着孔36と空気抜き孔37と
を有する上蓋35、点着孔36の間を連絡する繊維から
なる多孔性ブリッジ38を備える。
The ion activity measuring instrument of the present invention can take various specific embodiments, and for example, it can be of the j:4 structure described in JP-A No. 58-211648. for example,
As shown in FIG. 1, a sheet-like solid electrode (pair 26) having an ion-selective layer on its upper surface, an electrical connection terminal part 27, a support frame 28 for housing a plurality of solid electrode pairs (pairs), A water engineering 3S sheet member 30 for covering, a liquid supply hole 31 provided in the water-impermeable member 30, a porous member 32 that distributes liquid to the liquid gt supply hole 31, and a 2 for receiving the test liquid and reference liquid, respectively. A top lid 35 having two liquid storage Pa 34, a spotting hole 36 and an air vent hole 37, and a porous bridge 38 made of fiber that communicates between the spotting holes 36 are provided.

特開昭60−155960号、特開昭60−26084
3号、特開昭60−260844−弓に記載されたfl
it造にすることもできる。
JP-A-60-155960, JP-A-60-26084
No. 3, JP-A-60-260844-fl written on the bow
It can also be made from IT.

また特願昭60−148564号に記載された構造とす
ることができる。たとえば第2図に示すように、イオン
選択層を下面に有し両端に電気接続領域を有するシート
状固体電極対11 a、1 lb、llc、点着孔12
と空気抜き孔17とを有し、複数の固体′rrbf!対
を収納する上部枠体18、点着孔12の間を連絡する繊
維からなる多孔性ブリッジ19、固体電極対のイオン選
択層の下面に接して設けられた水不透性シーl一部材2
0、水不透性部材20に設けられた液供給孔13.15
a。
Further, the structure described in Japanese Patent Application No. 60-148564 can be used. For example, as shown in FIG. 2, a pair of sheet-like solid electrodes 11 a, 1 lb, llc, and a spotting hole 12 having an ion-selective layer on the lower surface and electrical connection areas at both ends.
and an air vent hole 17, and a plurality of solid 'rrbf! An upper frame body 18 that houses the pair, a porous bridge 19 made of fibers that communicates between the spotting holes 12, and a water-impermeable seal member 2 provided in contact with the lower surface of the ion-selective layer of the solid electrode pair.
0, liquid supply hole 13.15 provided in water-impermeable member 20
a.

15b、15c、液供給孔15a、1.5L+、15c
に液を分配する多孔性部材16、凹陥部14丙に多孔性
液分配部材16を収容する下部枠体21を備える。固体
電極対11a、llb、llc、上部枠体18、多孔性
ブリッジ19、水不透性シート部材20および多孔性液
分配部材16を除く部材(液供給孔、凹陥部等を含む)
は、被検液および参照液に対し各1対設けられる。
15b, 15c, liquid supply hole 15a, 1.5L+, 15c
A porous member 16 for distributing liquid to the recess 14 and a lower frame 21 for accommodating the porous liquid distributing member 16 in the recess 14 are provided. Members excluding the solid electrode pairs 11a, llb, llc, the upper frame 18, the porous bridge 19, the water-impermeable sheet member 20, and the porous liquid distribution member 16 (including liquid supply holes, recesses, etc.)
One pair each is provided for the test liquid and the reference liquid.

本発明のイオン活量測定2)具は、また特願昭60−1
80358号、特願昭60−180359号、特願昭6
0−180360号に記載されたような構造とすること
も出来る。
The ion activity measuring device 2) of the present invention is also used in Japanese Patent Application No. 60-1
No. 80358, Patent Application No. 180359, Patent Application No. 1983
It is also possible to have a structure as described in Japanese Patent No. 0-180360.

本発明のイオン活ri測定器具は、また第3図のような
構造とすることも出来る。図において、31 a、3 
l b、31 cはイオン選択層を下面に有するシート
状固体T、極対、38は点着孔51を有し、複数の固体
Th極対を収納する上部枠体、39は点着孔51の間を
連絡する繊維からなる多孔性ブリッジ、40は固体電極
対のイオン選択層の下面に接して設けられた水不透性部
材であり、水不透性部材40には液供給孔33’、35
a、35b、35cが設けられている。36は液供給孔
35a、35b、35cに液を分配する多孔性部材、/
11は下部枠体で、凹陥部34内に4つの部分から成る
多孔性液分配部材36を収容する。また下部枠体41に
は空気抜き孔37a、37bが設けられている。多孔性
ブリッジ3つは液供給孔の中心点から偏心した位置を通
っていてもよい。
The ion activity ri measuring instrument of the present invention can also have a structure as shown in FIG. In the figure, 31 a, 3
1 b, 31 c is a sheet-like solid T having an ion selective layer on the lower surface, pole pairs; 38 is an upper frame having spotting holes 51 and housing a plurality of solid Th pole pairs; 39 is a spotting hole 51; A porous bridge 40 made of fibers that connects the solid electrode pair is a water-impermeable member provided in contact with the lower surface of the ion-selective layer of the solid electrode pair, and the water-impermeable member 40 has liquid supply holes 33'. , 35
a, 35b, and 35c are provided. 36 is a porous member that distributes the liquid to the liquid supply holes 35a, 35b, and 35c;
Reference numeral 11 denotes a lower frame body which accommodates a porous liquid distribution member 36 consisting of four parts in a recessed part 34. Further, the lower frame body 41 is provided with air vent holes 37a and 37b. The three porous bridges may pass through positions eccentric from the center point of the liquid supply hole.

これらのイオン活量測定器具を用いて・Cオン活量を測
定するには、3111のイオン選択電極対をそれぞれナ
トリウム、カリウム、塩素各イオンに選択性の電極対と
し、参照液と被検液を液点着孔に点着すると、参照液と
被検液はそれぞれ多孔性液分配部材に浸透し、水不透性
部材に設けられた液供給孔を経て、各イオン選択電極の
表面に供給される。その結果、一対のイオ〉′選択電極
の間にそれぞれ電位差が発生するので、イオン選択電極
対の両端に設けられた電気接続領域を介して、電位差計
で電位差を測定すればよい。
To measure C-on activity using these ion activity measurement instruments, use the 3111 ion-selective electrode pairs as selective electrode pairs for sodium, potassium, and chlorine ions, and separate the reference solution and test solution. When the reference liquid and the test liquid are applied to the liquid spotting hole, the reference liquid and the test liquid permeate the porous liquid distribution member, and are supplied to the surface of each ion-selective electrode through the liquid supply hole provided in the water-impermeable member. be done. As a result, a potential difference is generated between the pair of ion-selective electrodes, and the potential difference can be measured with a potentiometer via electrical connection regions provided at both ends of the pair of ion-selective electrodes.

1体イオン選択電極としては、特開昭58−21164
8号、待17iJ昭60−237351号、特開昭60
−237352号、特開昭61−7460号、特開昭6
1−7461号、特開昭G1−7462号、特願昭60
 232306号に記載されたイオン選択電極を用いる
ことができる。
As a one-body ion selective electrode, Japanese Patent Application Laid-Open No. 58-21164
No. 8, 17iJ No. 1983-237351, JP-A-1983
-237352, JP-A-61-7460, JP-A-6
No. 1-7461, Japanese Patent Application Publication No. 1-7462, Patent Application No. 1983
The ion selective electrode described in No. 232306 can be used.

イオン選択電極は、特開昭58−10214G号、特開
昭58−156848号、特開昭60−243555号
に記載された方法で′IA造することが出来る。
The ion selective electrode can be manufactured by the method described in JP-A-58-10214G, JP-A-58-156848, and JP-A-60-243555.

[実施例1〕     (溶血の程度の比91)下記(
第1表)材↑Eiで出来た大きさ1.8crnX4C1
nの分配部材をそれぞれ血漿分離用の遠心管に入れ、ヘ
パリン採血した全血を500μlずつ加えた。1分間放
置後、分配部材を除き、遠心分1iiaにかけ、血漿を
得た。f3られな血漿中のカリウム・イオン濃度を炎光
光度計(コーニング モデル460)で測定した結果を
第1表に示す。
[Example 1] (Hemolysis degree ratio 91) The following (
Table 1) Size 1.8crnX4C1 made of material ↑Ei
Each of the n distribution members was placed in a centrifuge tube for plasma separation, and 500 μl of heparinized whole blood was added to each tube. After standing for 1 minute, the distribution member was removed and centrifuged 1IIA to obtain plasma. Table 1 shows the results of measuring the concentration of potassium ions in the blood plasma using a flame photometer (Corning Model 460).

第1表 第1表より医療用包帯、ラピアSは全血の展開性は良好
であるが、溶血の程度が大きいことがわかる。
From Table 1, it can be seen that the medical bandage Lapier S has good spreadability with whole blood, but the degree of hemolysis is large.

第1表でベンリーゼ(:S 303と100%ポリエス
テル布は溶血の程度が少ない、しかし、100%ポリエ
ステル布は明断が難しく、全血の展開性が十分でなかっ
た。ベンリーゼにS 303は溶血が少ないだけでなく
、指定の寸法に容易に切断でき。
Table 1 shows that Benliese (:S 303) and 100% polyester cloth had a low degree of hemolysis, but the 100% polyester cloth was difficult to separate, and the spreadability of whole blood was not sufficient. Not only is it small, but it can also be easily cut to specified dimensions.

展開も速かった。The development was also fast.

[実施例2]  (カリウム・イオン濃度測定)特願昭
59−244199号第一図に開示されたイオン活量測
定器具の中央の位置にカリウム・イオン選択電極を組み
込み、下表に示した分配布を用いて多項目イオン活量測
定器具を作製した。ヘパリン採血した全血50μlを試
料液とし、参照液としてカリプレート レベル−2(ワ
ーナー ランバート社 カリウム・イオン濃度4.91
11ea/l )  50μlを用い、点着1分後のカ
リウム・イオンj」択電極の電位値を測定した結果は第
2表の通りであった。
[Example 2] (Measurement of potassium ion concentration) A potassium ion selective electrode was incorporated in the center position of the ion activity measuring device disclosed in Figure 1 of Japanese Patent Application No. 1987-244199, and the concentration shown in the table below was incorporated. A multi-item ion activity measurement device was created using the distribution. 50 μl of heparinized whole blood was used as the sample solution, and the reference solution was Caliplate Level-2 (Warner-Lambert Co., potassium ion concentration 4.91).
Table 2 shows the results of measuring the potential value of the potassium ion selective electrode one minute after spotting using 50 μl of 11ea/l).

第2表 第2表において、本発明によるセルロース製繊f、Ii
不織布は全血でのカリウム・イオン濃度測定値が血漿で
の測定値とほぼ等しく、溶証が少ないことを示している
Table 2 In Table 2, the cellulose fibers f, Ii according to the present invention
The nonwoven fabric showed that the measured potassium ion concentration in whole blood was approximately equal to that measured in plasma, indicating less dissolution.

[実施例3] 第3図に示したイオン活量測定器具の4枚の液分配部材
36としてベンリーゼG5303を用い、多項目イオン
活量測定器具をf1τ製した。参照液としてカリプレー
ト レベル−2(ワーナー ランバート社 カリウム・
イオン濃度4.9 meq/I)50μ!を用い、ヘパ
リン採血した全血またはこの全血を遠心分離後の血漿5
0μlを試料液とし、カリウム・イオン濃度を測定した
。試料液各100検体についてカリウム・イオン濃度を
測定した結果、全血と血漿の測定値が0.5 meq/
I以上ずれるものは一つもなかった。
[Example 3] Using Benliese G5303 as the four liquid distribution members 36 of the ion activity measuring device shown in FIG. 3, a multi-item ion activity measuring device was manufactured by f1τ. Caliplate Level-2 (Warner-Lambert Co., Ltd. potassium) was used as a reference solution.
Ion concentration 4.9 meq/I) 50μ! Whole blood collected with heparin or plasma after centrifugation using
Using 0 μl as a sample solution, the potassium ion concentration was measured. As a result of measuring the potassium ion concentration for each 100 sample solutions, the measured value for whole blood and plasma was 0.5 meq/
There was no deviation of more than I.

【図面の簡単な説明】[Brief explanation of drawings]

第1図、第2図、第3図は本発明のイオン活量測定器具
の実施態様を示す分解斜視図である。 出願人    富士写真フィルム株式会社第  II!
FIGS. 1, 2, and 3 are exploded perspective views showing embodiments of the ion activity measuring instrument of the present invention. Applicant: Fuji Photo Film Co., Ltd. II!
I

Claims (6)

【特許請求の範囲】[Claims] (1)特定イオンに選択的に応答するイオン選択電極の
対と、前記電極の対のうち第一の電極に被検液を供給で
きる多孔性液体分配部材と、前記電極の対のうち第二の
電極に参照液を供給できる多孔性液体分配部材と、各電
極に供給される被検液と参照液との間に電気的導通を達
成するための多孔性ブリッジをそなえるイオン活量測定
器具であって、前記液体分配部材が長繊維セルロースよ
りなる不織布であることを特徴とするイオン活量測定器
具。
(1) a pair of ion-selective electrodes that selectively respond to specific ions, a porous liquid distribution member capable of supplying a test liquid to a first electrode of the pair of electrodes, and a second electrode of the pair of electrodes; An ion activity measuring device comprising a porous liquid distribution member capable of supplying a reference liquid to each electrode, and a porous bridge to achieve electrical continuity between the test liquid and the reference liquid supplied to each electrode. An ion activity measuring instrument characterized in that the liquid distribution member is a nonwoven fabric made of long fiber cellulose.
(2)イオン選択電極を少なくとも2対そなえることを
特徴とする特許請求の範囲(1)のイオン活量測定器具
(2) The ion activity measuring instrument according to claim (1), comprising at least two pairs of ion-selective electrodes.
(3)液体分配部材がコットンリンターを原料とする長
繊維からなることを特徴とする特許請求の範囲(1)の
イオン活量測定器具。
(3) The ion activity measuring device according to claim (1), wherein the liquid distribution member is made of long fibers made from cotton linters.
(4)液体分配部材がバインダーを実質的に含まないセ
ルロース系スパンボンド不織布であることを特徴とする
特許請求の範囲(1)のイオン活量測定器具。
(4) The ion activity measuring device according to claim (1), wherein the liquid distribution member is a cellulose spunbond nonwoven fabric that does not substantially contain a binder.
(5)液体分配部材がコットンリンターをシュバイツァ
液に溶解してなる紡糸液からスパンボンド法で製造した
不織布であることを特徴とする特許請求の範囲(1)の
イオン活量測定器具。
(5) The ion activity measuring device according to claim (1), wherein the liquid distribution member is a nonwoven fabric manufactured by a spunbond method from a spinning solution obtained by dissolving cotton linters in Schweitzer liquid.
(6)前記液体分配部材は3秒間に部材の重量の少なく
とも10倍の血液を吸収する多孔性部材であることを特
徴とする特許請求の範囲(1)のイオン活量測定器具。
(6) The ion activity measuring device according to claim (1), wherein the liquid distribution member is a porous member that absorbs at least 10 times the weight of blood in 3 seconds.
JP61019950A 1986-01-31 1986-01-31 Instrument for measuring ionic activity Granted JPS62177446A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP61019950A JPS62177446A (en) 1986-01-31 1986-01-31 Instrument for measuring ionic activity
US07/009,470 US4842712A (en) 1986-01-31 1987-02-02 Device for measuring ion activity
DE8787101366T DE3766835D1 (en) 1986-01-31 1987-02-02 DEVICE FOR MEASURING ION ACTIVITY.
EP87101366A EP0231033B1 (en) 1986-01-31 1987-02-02 Device for measuring ion activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61019950A JPS62177446A (en) 1986-01-31 1986-01-31 Instrument for measuring ionic activity

Publications (2)

Publication Number Publication Date
JPS62177446A true JPS62177446A (en) 1987-08-04
JPH0556819B2 JPH0556819B2 (en) 1993-08-20

Family

ID=12013482

Family Applications (1)

Application Number Title Priority Date Filing Date
JP61019950A Granted JPS62177446A (en) 1986-01-31 1986-01-31 Instrument for measuring ionic activity

Country Status (1)

Country Link
JP (1) JPS62177446A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077181A (en) * 1988-08-09 1991-12-31 Eastman Kodak Company Optical recording materials comprising antimony-tin alloys including a third element

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004053401A (en) 2002-07-19 2004-02-19 Fuji Photo Film Co Ltd pH ELECTRODE

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077181A (en) * 1988-08-09 1991-12-31 Eastman Kodak Company Optical recording materials comprising antimony-tin alloys including a third element

Also Published As

Publication number Publication date
JPH0556819B2 (en) 1993-08-20

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