JPS60155960A - Ion activity measuring instruments - Google Patents

Abstract

PURPOSE:To enable the measurement of ion activity at a high accuracy by providing a vent hole section with a porous bridge. CONSTITUTION:In an activity measuring instruments for three types of ions, ion selection electrode pairs 210, 220 and 230 comprising pairs of ion selection electrodes 211 and 212, 221 and 222, 231 and 232 the same in the type are arranged on a lower support frame 100. A non-water permeable member layer 30 having through holes 3100 (six in total) for passing liquid is arranged on the electrode pairs 210-230. A porous liquid portion member 41 is bonded on the layer to house liquid storage tanks 511 and 512. Three through holes each are provided on an upper frame 500 in such a manner as to communicate with the liquid storage tanks 511 and 512 and the through holes 5221 and 5222 serve as liquid supply hole and the remaining ones 5211, 5231, 5212 and 5232 as vent hole. A porous bridge 530 is provided on an area linking the holes 5231 and 5232 and a sunk area 550 in the extension thereof and the liquid being inspected and a reference liquid diffuse therethrough resulting in a potential difference corresponding to the activity of reference ion between the electrode pairs 210-230.

Description

[Detailed Description of the Invention] [Technical Field of the Invention] The present invention is a method for quantitatively analyzing the activity (or concentration) of specific ions in liquid samples, particularly biological fluids (blood, urine, saliva, etc.) by phototensiometry. Concerning an ion activity measuring instrument for.

[Prior art]

We use the droplet volume of liquid samples (tap water, river water, sewage, industrial wastewater, etc.) and biological fluids (blood (whole blood, plasma, serum), urine, saliva, etc.) to determine the concentration of specific ions contained therein. An ion activity measurement device that can measure the activity is published by JP-A-Shoj Co., Ltd.
112! Fl/, (US patent 013311), JP-A-Shoj! - Koo4I Tata, JP-A-Shozr-, 2iitμ, etc.

Among these, the ion activity measuring device disclosed in JP-A-111-21/1 IAI combines seven porous bridges with multiple pairs of solid ion-selective electrodes of the same or the same type. This was an epoch-making device that could measure the activity of several types of ions by simply supplying droplets of a reference liquid and a liquid sample once each. This ion activity measurement device has the appearance shown in Figure 1 (this figure shows i!) Three pairs of ion-selective electrodes (with an embedded device, the activities of three types of ions can be measured), and a twisted thread. The reference liquid and the total droplet amount of the liquid sample are supplied to each of the two through holes 6221 and 6222, which are provided with a porous bridge 530 made of porous liquid distribution member 41 made of gauze. and 42
(not shown) and reaches the portion corresponding to each electrode of the three pairs of electrodes and contacts the ion-selective layer, while on the other hand, both liquids diffuse and move through the porous bridge to form a porous bridge. At the center of the two liquids, the interfaces of both liquids touch and the electric current is generated.
electrical conduction is achieved, and a potential difference appears between each pair of ion-selective electrodes corresponding to the activity of the ions to be measured (three types in this case) in the liquid sample. In this device, the through holes provided with porous bridges also serve as liquid supply holes (each serving as a reference liquid and liquid sample supply hole), so that each of the two porous liquid distribution members for both liquids 7c The interface of both liquids comes into contact in the porous bridge at an earlier time than the time it takes to diffuse and reach each ion-selective layer, and then both liquids enter the other liquid near the contact interface of the two liquids. Due to mutual diffusion, mixing of both liquids □ occurs near the contact interface, and as a result, instability of the potential difference such as impression, disturbance, and large drift of the potential difference occurs, and the measured value of the activity of the target ion eventually stops. In some cases, the potential difference becomes -
It has been found that there is a problem in that it becomes impossible to set the

[Purpose of the Invention J The present invention solves the above-mentioned problems in the ion activity measurement device that can measure the activity t of multiple types of ions with one device, as shown in FIG. The purpose of the present invention is to provide an ion activity measuring instrument having a structure that allows measurement of ion activity.

(Structure of the Invention) The present invention includes (a) a lower support frame, and (b) a plurality of ion-selective electrode pairs disposed on the support frame, each of the electrode pairs having a first and a second electrode. Consists of an ion-selective electrode,
In each pair of electrodes, the first and second ion-selective electrodes are of the same type, but each set is of a different type; (d) a first porous liquid distribution member disposed over the seventh electrode of each set and a third electrode of each set through the through-hole of the water-impermeable member layer; a second porous liquid distribution member disposed over the electrode;
It has independent first and second □ liquid storage tanks in the lower part that can each accommodate three current distribution members, and at least one hole penetrating each of the liquid storage tanks is provided at a position corresponding to each of the electrodes. and an upper frame in which a porous bridge is provided between two through-holes corresponding to first and second electrodes of a pair of polarizations in the through-holes. In the measuring instrument, 1! of one set of said through holes. Two through-holes corresponding to the first and second electrodes of the pole pair are respectively used as liquid supply holes, and two of the remaining through-holes correspond to the first and second electrodes of one pair of electrodes. This is an ion activity measuring instrument in which a porous bridge is provided between through holes.

Next, the active M of three different ions shown in FIG. 2 (schematic diagram showing the appearance) and FIG. 3 (schematic diagram showing the structure), which are representative preferred embodiments of the present invention, were measured. The explanation will be based on the ion activity measuring instrument that can be used. As shown in FIG. 3, one of the ion-selective electrode pairs 210 also serves as a first ion-selective electrode 211 and a second ion-selective electrode 212, and the electrodes 211 and 212 are the same ion-selective electrode.

Similarly, the other ion-selective electrode pairs 2201 and 230 are electrodes 221 and 222, respectively; electrodes 231 and 232 are the same ion-selective electrodes. and three electrode pairs 210.22
0.230ij are mutually different ion selective polarization pairs. (Each electrode pair uses the ion-selective electrode pair disclosed in Japanese Patent Application Laid-Open No. 2003-100013.) These three ion-selective electrode pairs are arranged on the lower support frame 100.

A water-impermeable member layer (pressure-sensitive adhesive layer is provided on both sides. H+) ester is provided with through-holes a1oo (total 6 holes) for allowing liquid to pass over these three pairs of ion-selective electrodes. A film (so-called double-sided adhesive tape) 30 is placed. The water-impermeable member [aO] is provided with an adhesive layer on both sides, one surface of which is adhered to the ion-selective layer, and the other surface is adhered to porous liquid distribution members 41 and 42, which will be described later. There is. Between the water-impermeable material layer and the ion-selective layer, there is an adhesive layer (or a film-like adhesive layer with an adhesive layer) that does not adversely affect the ion-selective layer or has good affinity with the ion-selective layer. member) can be interposed.

The porous liquid distribution member (pure colored webbing or cotton gauze) is connected to the first electrode 211.221. of the three ion-selective electrode pairs.
231 into three through holes 3111.3.2□, 3.3. □
House 1. O/(Q, □ 1st material 41 and 2nd electrode 212.
222.232 with three through holes 3112.3122.3
The porous liquid distribution member 42 is made up of two porous liquid distribution members 42 that are covered with a tube 132 in between. It needs to be placed in Two multi-porous liquid distribution members 41 and 42 each have an upper frame (upper lid) which will be described later.
The liquid distribution member 5001C is housed in two independent liquid storage tanks 511 and 512 which also serve as liquid distribution member storage chambers. By being housed inside the liquid storage tank and surrounded by a water-impermeable member layer having an adhesive layer, the two porous liquid distribution members retain the liquid contained in each without contacting each other and do not penetrate through the liquid. Liquid can contact each ion selective layer through the holes. The upper frame (upper lid) SOO (made of molded electrically insulating organic polymer) has liquid storage tanks 511 and 5.
Three through holes each communicating with 12 (!-) are provided at positions substantially corresponding to the through holes provided in the water impermeable member layer 30.

Among these, through holes 5221 and 522214 in the center that communicate with the liquid storage tanks 511 and 512 are liquid supply holes through which liquids (reference liquid and liquid sample) are supplied to the porous liquid distribution members 41 and 42, respectively. , remaining through hole 5211,
5231; 5212.5232 Each butterfly has an air vent hole. Surroundings of the liquid supply holes 5221 and 5222 VCl-t each have a rising region 5 in which the thickness of the upper frame is larger on the upper side.
42 and 542, these raised areas prevent the liquid supplied from overflowing onto the upper surface of the upper frame and also act as guides to facilitate and ensure liquid supply. A concave area 550 is provided in the upper frame in the area connecting the two air vent holes 5231 and 5232 and its extension area, and the two air vent holes 5231 and 5232' (iH passes through the concave area 550). porous bridge (
A polyester fiber twisted yarn bridge 530 is provided with its both ends fixed (heat fused) near the edge of the upper frame. The remaining two air vent holes 5211 and 5212i$
It acts simply as an air vent.

Each of the above-mentioned components is a preferred/embodiment of the present invention having the appearance shown in FIG. 2 by joining and fixing (thermal fusion) the lower support frame and the upper frame so that their edges match. An ion activity measuring instrument capable of measuring the activities of three types of ions is obtained. In addition, the notches 1eo;56oH provided on the opposite sides of the lower support frame and the upper frame are located in matching positions, and have functions such as displaying the direction when using the instrument.
- Hatasu.

The lower support frame used in the ion activity measuring device of the present invention described based on the representative preferred embodiments described above,
Water-impermeable material layer, porous liquid distribution member, upper frame (upper lid)
, a porous bridge consisting of seven strands) can be used in the present invention according to the materials and processing methods disclosed in JP-A-11-2//441, respectively.

In particular, the upper frame (upper lid) is molded to include a liquid storage tank, through holes (liquid supply hole and air vent hole), a recessed area for locating the porous bridge, and, if necessary, a recessed area for fixing the porous bridge. By forming an integral member with a heat-sealing portion or a receiving hole for a fixing stopper, the device of the present invention can be assembled easily and reliably, and is easy to operate during use.

It goes without saying that the ion activity measuring instrument of the present invention is not limited to the number of ion-selective electrode pairs Fi, but can be implemented with two or more pairs of ion-selective electrodes. Furthermore, the present invention can be modified in various ways without departing from its basic technical concept, and if necessary, a magnetic code or bar code may be provided to display the ion species to be measured. can do.

〔Effect of the invention〕

As explained above, by not providing a porous bridge at the liquid supply hole but by providing a porous bridge at the air vent hole, after supplying the reference liquid and liquid sample, each liquid reaches the ion selective electrode and bridges. The contact between both liquids in the bridge is well controlled, and the contact interface between the two liquids in the bridge is stable during potential difference measurement, so potential fluctuations and drifts are extremely reduced, improving the accuracy of ionic activity measurement. The effect of improving repeatability is also achieved.

Furthermore, as a side effect, when supplying liquid to the liquid supply hole, the liquid supply/vessel equipment such as a micropipette or liquid supply tube does not come into contact with the porous bridge, especially the thin twisted thread bridge, making it easier to operate. This has the advantage that it is easy to use, and there is no risk of the liquid supply air damaging the bridge.

[Brief explanation of drawings]

FIG. 1 is a schematic perspective view of a conventional ion activity measuring instrument capable of measuring the activities of three types of ions. FIGS. 2 and 3 are diagrams showing an ion activity measuring instrument capable of measuring the activity i of three types of ions, which is a preferred embodiment of the present invention. FIG. 2 is a schematic perspective view of the external appearance, and FIG. 3 is a schematic exploded perspective view for explaining the configuration. 100... Lower support frame 210.220.230... Three ion selection electrode pairs 211.221.231... First ion selection electrode 2
12.222.232...first ion selection electrode 30
...Water-impermeable member layer 3100; 3111.3121, 3131.3112., which has through holes and has adhesive layers on both sides.
3122.3132...Through hole 41;42...7th
, and the first porous liquid distribution member soo; soo...upper frame (upper lid s1'i; s12...first and second
Liquid storage tank 6221.5222; +22x, a 222
...Liquid supply hole 5211.5231.5 '212.52az; 5i1
1.6231.6212.6232... Air vent S a O = - Porous bridge (twisted bridge) 53
9... Twisted thread bridge fixing part (heat fusion) 639...
Twisted thread bridge fixing part (hole and plug) 541, S42...
Raised area sso...Concave area 160.560...Notch to indicate direction of use, etc. Patent applicant: Fuji Photo Film Co., Ltd. Engraving of the drawing (no change in content) Figure 1 II 2 Figure 113 ;4) 230 Procedural amendment dated March 9, 1927, Mr. Commissioner of the Japan Patent Office 1, Indication of the case, 1920 patent application No. 117u4A 2,
Title of the invention Ion activity measuring device 3, relationship with the case of the person making the amendment Patent applicant Location 210 Nakanuma, Minamiashigara City, Kanagawa Prefecture Name (52)
0) Fuji Photo Vilm Co., Ltd. Telephone (406) 2537
'〜°−' 4. Subject of amendment Drawing 5. Details of amendment We will submit an engraving of the drawing (no changes to the content). Procedural amendment (method) Showa! New year! Mr. Commissioner of the Japan Patent Office, 1, Indication of the case, 1920 Showa JR Patent Application No. 7, Department No. 2, Invention 1, Ion activity measuring device 3, Relationship with the amended person case, Patent applicant 4, Date of amendment order Showa! January ≠ 85, we will submit a revised version of the amended specification 6 (no changes to the content).

Claims (4)

[Claims] (1) (a) Lower support frame, (b) Arranged above the support frame! a plurality of ion-selective electrode pairs, each of which includes a first and a second ion-selective electrode;
Although the ion-selective electrodes are of the same type, each set is of a different type. (c) a water-impermeable member layer having one through hole at a position corresponding to each of the electrodes; a first porous liquid distribution member disposed over the first electrode and a second porous liquid distribution member disposed over the second electrode of each set;
a liquid distribution member, (e) having independent first and first liquid storage tanks at the lower part capable of respectively accommodating the two liquid distribution members, and having at least one of the liquid storage tanks at a position corresponding to each of the electrodes; an upper portion having a hole penetrating the liquid reservoir, and a porous bridge provided between one through hole corresponding to the first and second electrodes of the seven electrode pairs; In an ion activity measuring instrument having a frame, twelve through-holes corresponding to the first and second electrodes of one pair of electrodes among the through-holes are respectively set to supply a liquid, and the remaining An ion activity quantification device characterized in that a porous hole is provided between two of the through holes corresponding to the first and first electrodes of one pair of electrodes. (2) The ion activity measuring device according to claim 1, wherein the porous bridge is a twisted yarn made of fibers. (3) Around the two liquid supply holes, the upper frame has a raised region whose thickness increases toward the upper side, and the porous bridge is formed in a recessed region toward the lower side of the upper frame. The ion activity measuring instrument according to claim 1 or 2, wherein the ion activity measuring instrument is arranged. (4) The water-impermeable member layer is coated with adhesive on both surfaces.
the lower adhesive layer adheres to the top layer of each electrode around the through-hole of the water-impermeable material layer, and the upper adhesive layer adheres around the two liquid storage layers. The ion activity measuring instrument according to claim 1, which is adhered to the lower surface of the upper frame.