JPS62158480A - Method for processing raw test specimen - Google Patents

Abstract

PURPOSE:To prevent various germs from invading a zone of the aimed raw test specimen, by irradiating the surroudings of the raw test specimen to be processed with sterilizing light rays and preventing invasion of various germs. CONSTITUTION:At a calculation treatment part 14 control actions to carry out processing of raw test specimen is done depending upon content of an image memory 12 and indication position detected, by a light pen 13'. At the calculation treatment part 14, a zone except a zone wherein the raw test specimen exist in a container, namely the surroundings of existing zone of the raw test specimen is subjected to control wherein surroundings are irradiated with ultraviolet rays (sterilizing light rays) generated from an ultraviolet light source 30 and various germs are destroyed. Consequently, the various germs can be prevented from invading the existing zone of the aimed test specimen.

Description

DETAILED DESCRIPTION OF THE INVENTION [Uses of the Invention] The present invention relates to a raw sample processing method for performing necessary processing on a raw sample by irradiating the raw sample with a solid line for processing.

[Invention! 'Scenery]

It is generally known that processing light (laser light, ultraviolet light, etc.) can be used to process raw samples such as cells and microorganisms (bacteria, viruses, etc.). refers to amputation, perforation, etc.

The technology for processing cells etc. using laser light was developed in 1974.
Technical magazine published in November [Science Mo1
．． 186J (8CIINCE vol,
186) P700-P705, and the technical magazine "Science ■ol, 21, published on July 31, 1981.
P2O of 3J (8CIENCI3 Mo1.213)
3 to P513, etc.

[Purpose of the invention]

An object of the present invention is to provide a method for processing a raw sample that can prevent bacteria from entering the area of the target raw sample.

[Requirements for invention]

The present invention provides for the surrounding area of a raw sample to be processed to be
It is characterized by irradiating germicidal light to prevent the invasion of germs, etc.

[Embodiments of the invention]

Hereinafter, the present invention will be explained in detail based on specific examples.

FIG. 1 shows a system according to one embodiment of the invention. m
In Figure 1, l is a sample holder that holds the raw sample to be processed, 2 is a stage, 3 is a lamp for transmitted illumination, 4 is a condenser lens, 5 is a mirror, 6 is a deflection device that deflects the incident light beam, Reference numeral 7 indicates a two-dimensional scanning unit that adjusts the deflection device 6, and reference numeral 8 indicates a stage position driving unit that moves the stage 2 in two-dimensional directions of X-Y. 9 is a microscope device. lO is the camera,
10' is an eyepiece, U is an A/D converter that converts an analog image (1) into a digital image signal, 12 is an image memory that stores the digital image signal, 13 is a monitor/TV, and 13' is a light pen. Reference numeral 14 denotes an arithmetic processing section, and the arithmetic processing section 14 performs control operations for processing the raw sample based on the contents of the image memory and the indicated position detected by the light pen 13'. The calculation processing unit 14 in the container (retainer) 1
Control is performed to irradiate a sterilizing light beam (sterilizing light beam) to a region outside the fB region where the raw sample exists, that is, around the region where the raw sample exists.

This process will be described later. A laser light source generates a light beam, in this case a laser beam, for processing a raw sample. 21 irradiates the raw sample with laser light (ON)
Alternatively, it is a shutter for non-irradiation (OFF). n
is the beam sublifter. The chrysanthemum is used to generate light rays (sterilizing rays) to kill germs and the like, and here it is an ultraviolet light source that generates ultraviolet rays. A shutter 31 turns on and off the ultraviolet rays. The support is the beam position adjuster.

The operation of the device shown in the m1 diagram is as follows. First, a raw sample to be processed is cultured in a holder 1 and is deposited on a cover glass attached to a window provided above or below the holder 1. When culturing a live sample, this cover glass is attached to the window of the holder I using a substance that does not dissolve in the culture medium, such as grease, and cells are cultured on this cover glass. A raw sample such as cultured m-cells is placed on the stage 2 along with the holder 1.

In this case, as shown in Figure ff12, the cover glass is placed so that each position can be observed with the microscope bag WL9. In FIG. 2, 41 is a region where cells to be processed exist (this region may be arbitrarily determined by the operator,
Alternatively, it may be determined after actually observing with the microscope bag fi9. ) is shown.

By the way, as mentioned above, the outside and surrounding area of the cover glass is in direct contact with the outside air, and bacteria etc.
There is a possibility that it may enter the interior through the gap between the glass and the retainer. Rather, it is more natural to think that bacteria and the like are invading the area around the cells to be processed. Therefore, in this embodiment, the ultraviolet rays generated from the ultraviolet light source I are used to make the area 4 in FIG. The surrounding area is irradiated with ultraviolet light to kill germs. When the holder 1 is placed on the stage 2 as shown in FIG.
captures the enlarged image obtained through the microscope device t9, and
The image is stored in the image memory via D conversion 'a11. Prior to laser machining or during the machining period, the arithmetic processing unit 14 determines whether the center position under observation is within or outside the region 41 in FIG. 2, using image signals stored in the image memory. to judge. This can be achieved using normal image processing techniques. In other words, for example, if the edge of the cover glass 40 is found through image processing, it is possible to distinguish between the inside and outside of the area 41 by specifying that a position shifted by a certain distance from this position is the boundary point of the area 41. is possible. If a program for performing such processing is stored in the arithmetic processing section 14, it can be determined whether the object is inside or outside the area 41 described above. The arithmetic processing section 14 can arbitrarily move the stage 2 in the X-Y directions by outputting a position control signal to the stage position driving section 8. By this movement, the position of the cover glass and the position of the cells can be relatively moved to the desired position of the image. Therefore, along with this movement, the arithmetic processing unit 14 opens the shutter 31 outside (around) the area 41 and emits ultraviolet rays at 32.22.
6. 4 to the holder l on the stage. This irradiation kills germs around the cover glass chrysanthemum, especially around the area where the live sample is present. this is,
This is extremely useful in preventing contamination when culturing raw samples after laser processing. It is also effective in removing unintended raw samples.

In addition, processing of the raw sample by laser exposure is performed using a 5-t arithmetic processing device.
14 is cyqy 4gllh, laser beam is n, 6°4
This is done by irradiating the raw sample through the irradiation method.

In the above-mentioned embodiments, it has been described that germs outside the area where the raw sample exists are killed by ultraviolet rays.

This takes advantage of the fact that ultraviolet light has a germicidal effect. Note that extermination of female teeth using ultraviolet rays can be achieved without using the method described above. That is, a shade may be attached around the condensing lens portion of the microscope device 9, and only the area around the sample may be irradiated with a germicidal lamp from outside.

Next, another embodiment of the present invention will be described using FIG.

Since each device with the same reference numeral as in the above-described embodiment is the same, a description thereof will be omitted. In Figure 3, the illusion is the tlJ! of the laser beam! This is a strong a regulator that adjusts L. That is, by providing this strength adjuster, it is possible to adjust the strength within the region where the raw sample exists to the strength that followed the processing thereof, and to adjust the strength outside the region to the level suitable for killing germs.

In the area 41 in FIG. 2, the arithmetic processing unit 14 controls the intensity adjuster n so that it can output a laser beam suitable for processing a raw sample. Outside the area 41, the intensity controller ntl controls the laser beam so that it can output enough laser light to exterminate germs. In this embodiment, since laser light from a laser light source provided for processing is used, the apparatus is simple.

As mentioned above! In the embodiment shown in Figure @3, the intensity of the laser beam is adjusted, but the present invention can be similarly realized by changing the irradiation time of the laser beam inside and outside the target area. That is, within the region 41 in FIG. 2, the shutter is opened for the laser light irradiation time suitable for processing, and outside the region 41, the shutter is opened so that the laser light irradiation time is suitable for exterminating (killing) germs. open. In this case, the strength adjusting device shown in FIG. 3 is also unnecessary, and the structure becomes even simpler.

〔Effect of the invention〕

As explained above, according to the present invention, it is possible to prevent bacteria and the like from entering the region where the target raw sample exists.

[Brief explanation of drawings]

FIG. 1 is a diagram showing one embodiment of the invention, FIG. 2 is a diagram for explaining the invention in detail, and FIG. 3 is a diagram showing another embodiment of the invention. 1... Sample holder, 2... Stage,
3... Lamp, 4... Condensing lens, 5...
...Mirror, 6...Deflection device, 7...
...Two-dimensional scanning unit, 8... Stage position drive unit, 9... Microscope device, lO... Camera, 11... Person/D conversion device, 12... image memory, 13... monitor/television, 13'.
...Light pen, 14... Arithmetic processing unit,
m...Laser light source, 21...Shutter, n...Beam splitter, addition...Ultraviolet light source, 31...Shutter,! ...Beam position adjuster, illusion...Intensity adjuster

Claims (1)

[Claims] 1. In a raw sample processing method in which a raw sample is irradiated with processing light to process the raw sample, sterilizing light is irradiated around the area where the raw sample to be processed exists to prevent the invasion of germs, etc. A raw sample processing method characterized by: