JPS62143687A - Inclusion immobilization method - Google Patents
Inclusion immobilization methodInfo
- Publication number
- JPS62143687A JPS62143687A JP60285352A JP28535285A JPS62143687A JP S62143687 A JPS62143687 A JP S62143687A JP 60285352 A JP60285352 A JP 60285352A JP 28535285 A JP28535285 A JP 28535285A JP S62143687 A JPS62143687 A JP S62143687A
- Authority
- JP
- Japan
- Prior art keywords
- microorganisms
- microorganism
- polyvinyl acetate
- mixture
- immobilization method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Biological Treatment Of Waste Water (AREA)
- Treatment Of Biological Wastes In General (AREA)
Abstract
Description
【発明の詳細な説明】
産業上の利用分野：
末完ＦＪ］は、微生物、場合によっては、微生物の含む
酵素を包括固定化する方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] Seikan FJ] relates to a method for entrapping immobilization of microorganisms and, in some cases, enzymes contained in microorganisms.
本発明は微生物というのは例えば活性汚泥法で使用され
るＰｓｅｕｄｏｌｎｏｎａｓ％Ｆｌａｖｏｂａｃｔｅｒ
ｉｕｍ、 ＡｃｈｒｏｎｎｂａｃＬａｓｐなどである。In the present invention, microorganisms include, for example, Pseudolnonas% Flavobacter used in activated sludge method.
ium, AchronbacLasp, etc.
本発明で酵素というのは例えば、アスパルターゼ、アミ
ノアシラーゼ、グルコ−女オキシラーゼ、カタラーゼ、
セルラーゼ等である。In the present invention, enzymes include, for example, aspartase, aminoacylase, glucooxylase, catalase,
Cellulase etc.
従来技術：
微生物を固定化する方法として、ゲルで包括する方法が
知られている［例えば、子細ほか：固定化酵素、高分子
＠２９巻３月号（１９８０）、Ｂｅｒｎｆｅｌｄ ｅｔ
ａｌ、 ； ５ｃｉｅｎｃｅ第１４２巻、６７８頁（１
９６３）、、Ｎ１５ｈｉｄａ ｅｔ＠１ 、 Ｂｉｏｔ
ｅ６ｋ 、 Ｂｉｏｅｎｇ 、 、第２１巻１６９７頁
（１９７９）などにその記載がある。］。これらの中で
、合成高分子の一種であるポリアクリルアミドゲルや、
天然ポリマーであるマンナン特にカラギーナンのゲルに
ついての記載がある。Prior art: As a method of immobilizing microorganisms, a method of entrapping them in a gel is known [for example, Kosa et al.: Immobilized Enzymes, Polymers @ Vol. 29, March issue (1980), Bernfeld et al.
al, ; 5science Vol. 142, p. 678 (1
963), N15hida et@1, Biot
It is described in e6k, Bioeng, Vol. 21, p. 1697 (1979). ]. Among these, polyacrylamide gel, which is a type of synthetic polymer,
There are descriptions of gels of the natural polymer mannan, particularly carrageenan.
解決しようとする問題点：
ポリアクリルアミドグルは加水分解して一部アクリルア
ミドモノマーを生じるので、微生物を死滅させる恐れが
あり、カラギーナンゲルの場合、その溶液は常温で固化
し、温度を５０’〜６０°に上昇させると微生物が死滅
し易い欠点がある。Problems to be solved: Polyacrylamide gel hydrolyzes to produce some acrylamide monomers, which may kill microorganisms.In the case of carrageenan gel, the solution solidifies at room temperature, and the temperature is raised to 50'-60°C. There is a disadvantage that microorganisms are easily killed when the temperature is raised to 15°C.
問題点を解決するｔめの手段：
本発明Ｃは、包括剤としてポリ酢酸ビニルの低重合物（
重合度１０００〜３０００、高粘度液）ｆ、用いる。す
なわち−微生物に対し、このものを加えて常温まｔけ微
生物の生息を妨げない加温下で攪拌混合し、空気中で放
置して重合を進め固化する。Tth means for solving the problem: The present invention C uses a low polymer of polyvinyl acetate (
Polymerization degree 1000-3000, high viscosity liquid)f is used. That is, - the product is added to the microorganisms, left at room temperature, stirred and mixed under heating that does not inhibit the inhabitation of the microorganisms, and left in the air to proceed with polymerization and solidify.
重合速度を増す九め、空中放置のかわりに、水に浸漬し
ても良い。水中ＩＣ分赦した微生物の場合、遠心分離・
濾過などの操作で微生物を濃縮しておいた方が、同化速
度が大である。Nineth, to increase the polymerization rate, instead of leaving it in the air, it may be immersed in water. In the case of microorganisms subjected to underwater IC, centrifugation and
The assimilation rate is faster if the microorganisms are concentrated through operations such as filtration.
前記含括処理のための条件は、微生物（乾燥物基準）１
重量品に対し、１〜１０重量部のポリ酢酸ビニルを加え
、１時間以内、望ましくは１．５〜３０分間撹拌する。The conditions for the above-mentioned comprehensive treatment are microorganisms (dry matter basis) 1
Add 1 to 10 parts by weight of polyvinyl acetate to the weight product and stir for within 1 hour, preferably for 1.5 to 30 minutes.
生成物は、空中放置の場合半日〜１日、水中浸漬の場合
１０分〜１２０ＩＩ＋で固化する。The product solidifies in half a day to one day when left in air, and in 10 minutes to 120II+ when immersed in water.
水中浸漬の場合、水温は低い方が固化し易り、５℃で５
分〜６０分で充分である。When immersed in water, the lower the water temperature, the easier it will solidify.
Minutes to 60 minutes is sufficient.
作 用：
微生物がポリ酢酸ビニルにより包括されると微生物の生
命維持は、ポリ酢酸ビニル層を通しての基質および、代
謝産物の拡散により行われる。Action: When microorganisms are encapsulated in polyvinyl acetate, their life is supported by the diffusion of substrates and metabolites through the polyvinyl acetate layer.
包括物質としてのポリ酢酸ビニルは、液状で、微生物と
混合しｔ後、重合して固化するが、基質および代謝産物
に対する拡散抵抗が小さり、シかも包括作用が勝れてい
る。Polyvinyl acetate as an entrapping material is in a liquid state, and after being mixed with microorganisms, it polymerizes and solidifies, but it has low diffusion resistance to substrates and metabolites, and has an excellent entrapping effect.
実施例：
第１図におりて、微生物と、重合度２０００のポリ酢酸
ビニルを、重量比で１：５の割合Ｃ混合槽＋１）へ注入
し、撹拌機＋２１で１０分間混合する。生成物はポンプ
（３）により水槽（４）に導き、パン状板（５）へ吐出
する。常温で約３０分浸漬状態を続けるとフィルム状も
しくは板状製品が得られる。Example: In FIG. 1, microorganisms and polyvinyl acetate having a degree of polymerization of 2000 are poured into a mixing tank C (+1) at a weight ratio of 1:5 and mixed for 10 minutes using a stirrer +21. The product is led to a water tank (4) by a pump (3) and discharged onto a pan-shaped plate (5). If the soaking state is continued for about 30 minutes at room temperature, a film-like or plate-like product can be obtained.
発明の効果：
本発明は微生物を、低重合ポリ酢酸塩化ビニルが空気中
ま乏ホ水中Ｃ１さらに重合を起こすことを利用し、簡単
な操作で、安価に包括・固定化する点Ｃメリットがある
。微生物のかわりに、微生物の行う生物作用中、化学反
応を直接仲介する酵素を用いても良く、相似の効果が得
られることは言うまでもない。また、ポリ酢酸ビニルは
、微生物の生息に悪影響を与えない。Effect of the invention: The present invention utilizes the fact that low-polymerized polyvinyl acetate chloride further polymerizes in water that is scarce in the air, and has the advantage of being able to encapsulate and immobilize microorganisms with simple operations and at low cost. . It goes without saying that enzymes that directly mediate chemical reactions during the biological actions performed by microorganisms may be used instead of microorganisms, and similar effects can be obtained. Furthermore, polyvinyl acetate does not adversely affect the habitat of microorganisms.
第１図は本発明実施の１例を示す工程図である。
＋ｉ）・・・混合槽 １２）・・・撹拌機 （３）
・・・ポンプ＋４１−・・水槽 （６）・・・パン状
板。
出願人 株式会社 タ り マ
第１図FIG. 1 is a process diagram showing one example of implementing the present invention. +i)...Mixing tank 12)...Agitator (3)
... Pump +41- ... Water tank (6) ... Bread-shaped plate. Applicant: Tarima Co., Ltd. Figure 1
Claims (1)
は水中で、該微生物が死滅しない程度の低温度に保持し
、さらに重合させることを特徴とする包括固定化方法。 ２ 該微生物が死滅しない程度の低温度が２℃〜３５℃
である第１項に記載の包括固定化方法。 ３ 微生物のかわりに、酵素を用いた第１項または第２
項に記載の包括固定化方法。[Scope of Claims] 1. An entrapping immobilization method characterized by mixing microorganisms and polyvinyl acetate, maintaining the mixture in air or water at a low temperature that does not kill the microorganisms, and further polymerizing the mixture. 2. The temperature is low enough that the microorganisms will not die from 2°C to 35°C.
The comprehensive immobilization method according to item 1. 3 Paragraph 1 or 2 using enzymes instead of microorganisms
Comprehensive immobilization method described in Section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60285352A JPH0634718B2 (en) | 1985-12-16 | 1985-12-16 | Comprehensive immobilization method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60285352A JPH0634718B2 (en) | 1985-12-16 | 1985-12-16 | Comprehensive immobilization method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62143687A true JPS62143687A (en) | 1987-06-26 |
| JPH0634718B2 JPH0634718B2 (en) | 1994-05-11 |
Family
ID=17690447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60285352A Expired - Fee Related JPH0634718B2 (en) | 1985-12-16 | 1985-12-16 | Comprehensive immobilization method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0634718B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7192765B2 (en) | 2002-01-25 | 2007-03-20 | Hitachi Plant Engineering & Construction Co., Ltd. | Method for producing a nitrification carrier and for removing nitrogen |
-
1985
- 1985-12-16 JP JP60285352A patent/JPH0634718B2/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7192765B2 (en) | 2002-01-25 | 2007-03-20 | Hitachi Plant Engineering & Construction Co., Ltd. | Method for producing a nitrification carrier and for removing nitrogen |
| US7655455B2 (en) | 2002-01-25 | 2010-02-02 | Hitachi Plant Technologies, Ltd. | Method of producing a nitrification carrier containing ammonia-oxidizing bacteria for removing nitrogen |
| US7704733B2 (en) | 2002-01-25 | 2010-04-27 | Hitachi Plant Technologies, Ltd. | Nitrogen removing apparatus comprising nitrification carrier containing ammonia-oxidizing bacteria |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0634718B2 (en) | 1994-05-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |