JPS62115365A - Reagent for detecting bad breath and method and instrument for measuring bad breath - Google Patents

Abstract

PURPOSE:To quickly and easily measure bad breath with high accuracy by using bis(4-dialkylaminophenyl)methanol (where, the alkyl group excludes a methyl group) or bis(4-alkylideneaminophenyl)methanol as a reagent. CONSTITUTION:The test paper is prepd. by impregnating the reagent contg. the bis(4-dialkylaminophenyl)methanol expressed by formula I and/or the bis(4- alkylideneaminophenyl)methanol expressed by formula II to a carriage 2 such as filter paper. The carrier 2 dropped with the saliva contg. mercaptan which is the bad breath component is imposed on a transparent plate 3 and a light emitting element 4 is made to emit light. The light reflected by the carriage 2 is received by a light receiving element 6 and is passed through an amplifier and an analog-to-digital converter 7. The intensity of the reflected light is displayed 8. The correspondence of the functional evaluation of the bad breath to the increase rate of the intensity of the reflected light measured by using the test paper is thus objectively executed by using the prescribed regression equation preliminarily determined from the relation between the concn. of the mercaptan and the increase rate of the intensity of the reflected light.

Description

Detailed Description of the Invention (Industrial Field of Application) The present invention relates to a halitosis detection reagent, a single odor measurement method, and an apparatus therefor, in particular, a method that enables dentists and dental hygienists to immediately diagnose bad breath in the examination room. The present invention relates to a quick, high-intensity, and high-accuracy halitosis detection reagent 2-b odor measurement method that enables a simple check of halitosis, and an apparatus therefor.

(Prior art) It is well known that hydrogen sulfide and methyl mercaptan, which are produced by the decay of sulfur-containing proteins in the oral cavity, are the main causes of bad breath (accounting for about 90% of bad breath).
For example, 0ral Surg, + Vol, 45°N
o, 4. P, 560-567 (1978)).

In the dental field, since patients are rarely confused about their own bad breath, doctors have a desire to inform patients of their own bad breath using some kind of measured value (quantitative value). Furthermore, dental hygienists have a desire to display the level of oral cleanliness, which is the result of brushing instructions for the teeth they are in charge of, using a simple method or device. On the other hand.

In cases of autohalitosis, where people mistakenly perceive their own physiological bad breath as a pathological bad odor, it is necessary to convince them by showing measured values that there is no such bad odor.

Measuring halitosis is based on two methods, as the main component of halitosis is sulfur compounds.
It is desirable to measure the total sulfur content. The most common method for this purpose is to oxidize sulfur compounds to produce sulfur dioxide gas, which is then measured by a chemical method, solution conductivity method, infrared gas analysis method, detector tube method, or the like. However, all of these measurement methods require a complicated pretreatment operation called oxidation treatment.

Examples of methods for directly measuring sulfur compounds without requiring such pretreatment include the above-mentioned OralSurg,
discloses a method for quantifying the concentration of volatile sulfur compounds in exhaled breath using gas chromatography. Gas chromatography analysis is excellent in its sensitivity and quantitative performance, but it is expensive and requires special instruments to collect samples (exhaled breath), making it difficult for dentists and dental hygienists who require halitosis analysis. I don't fit in. Arch, 0ral
Biol, Vol, 9+P, 39-45 (1964
) discloses that saliva is cultured and the concentration of sulfur-containing compounds produced in the medium is measured by gas chromatography or sensory testing. Although this method is easy to collect saliva samples, it requires a long time to culture and also employs gas chromatography and sensory testing, so it cannot be called a quick method for measuring halitosis. Furthermore, JP-A No. 57-135360 discloses that 4,4'-bisdimethylaminodiphenyl rubitol releases carbonium ions under acidic conditions, exhibits a blue color, and fades when it reacts with thiol groups. , discloses that mercaptan can be calculated by adding saliva to this compound and quantifying the degree of discoloration using a colorimetric method or a test paper method. Although this method has excellent accuracy, it takes a long time of about 30 minutes and requires a colorimeter, so it is not suitable for measuring halitosis in a consulting room where dentists and dental hygienists are present. The test strip method has low sensitivity for compounds.

Quantitative judgment with the naked eye is impossible.

(Problems to be Solved by the Invention) The present invention solves the above-mentioned conventional problems, and its purpose is to provide a highly sensitive halitosis detection reagent 1-bodor measuring method and an apparatus therefor. It is in. Another object of the present invention is to provide a halitosis detection reagent that can easily measure halitosis with high precision in a short period of time.

An object of the present invention is to provide a halitosis measuring method and a device therefor.

(Means for Solving the Problems) The present invention provides bis(4-
dimethylaminophenyl)methanol (BDC-OH,
Bis(4-bis(dimethylamino)henshydrol or Michler-Hydrol)
-dialkylaminophenyl)methanol (the alkyl group excludes the methyl group) or bis(4-alkylideneaminophenyl)methanol increases the sensitivity of the reagent and therefore allows for a shorter period of time compared to traditional halitosis assays. Bad breath can be easily measured with high precision. It was completed based on the inventor's knowledge.

The halitosis detection reagent of the present invention comprises bis(4-dialkylaminophenyl)methanol represented by formula 1 and/or
Bis(4-alkylideneaminophenyl) represented by the formula
It contains methanol, thereby achieving the above objective.

Here, R1 is an alkyl group having 2 or more carbon atoms, and R1 is an alkyl group having 2 or more carbon atoms.
z is alkylidene The halitosis measurement method of the basic invention is (bis(4) shown by the formula 111)
-Dialkylaminophenyl)methanol and/or bis(4-alkylideneaminophenyl)methanol represented by the formula A process of reacting with a certain mercaptan.

OH Here, R1 is an alkyl group having 2 or more carbon atoms H Here, R2 is an alkylidene group (2) The support is irradiated with light having the maximum absorption wavelength of the reagent, and the intensity of the reflected light from the support is measured. and (3) applying the measured values to a calibration curve showing the relationship between the rate of increase in reflected light and mercaptan concentration obtained in advance from steps (1) and (2) above to determine the mercaptan in the sample saliva. Including the step of reading the concentration.

This achieves the above objective.

The halitosis measuring device of the present invention has a screw (111 type) (
4-dialkylaminophenyl) methanol and/or a carrier impregnated with a halitosis detection reagent containing bis(4-alkylideneaminophenyl)tutanol represented by formula 1.

(ill) Here, R1 is an alkyl group H having 2 or more carbon atoms. Here, RZ is an alkylidene group. (2) a light emitting element that emits light in the maximum absorption wavelength region of the reagent to the support; (3) A light-receiving element having sensitivity in the wavelength range and receiving reflected light from the carrier of the light of the light-emitting element; A halitosis measuring device comprising a display means for displaying a change in the intensity of reflected light received by the light receiving element.

Bis(4-dimethylaminophenyl)methanol CBD
C-0) 1) is known for colorimetric determination of mercapto groups in proteins.
ry) 52, 127-142 (1973)). However, since this bis(4-dimethylaminophenyl)methanol (BDC-OH) has low sensitivity, it is impossible to use it in the form of a test paper to quantify color changes with the naked eye. Therefore.

The above bis(4-dimethylaminophenyl)methanol (
An attempt was made to improve the sensitivity by substituting a part of BDC-OH) with other groups. If 9, for example, a benzene ring is substituted with a group other than a methyl group, the electronic state of the compound will change significantly, which may result in the loss of deep blue coloration due to carbonium ions or discoloration due to reaction with thiol groups, as described below. There is. Therefore, we considered a compound of the present invention in which only the methyl group was substituted with another group.

In the acidic region, the compound of the present invention has D'! It becomes a bis(4-dialkylaminophenyl)methyl cation shown by the formula or a bis(4-alkylideneaminophenyl)methyl cation shown by the formula (2), and exhibits a deep blue color.

Here, R1 and R2 are the same as defined above.

This carbonium ion reacts with a compound having a mercapto group (R-3l+) to form an alkylbis(4-dialkylaminophenyl)methyl sulfide represented by the formula (■) or an alkylbis(4-alkylideneamino) represented by the formula (■). (phenyl) methyl sulfide and discolors. Therefore, a compound having a mercapto group can be quantified by irradiating carbonium ions with light of a certain wavelength and measuring the degree of light absorption.

S + R1 and R2 are the same as defined above.

R is an alkyl group.

The absorption wavelength of the above bis(4-dialkylaminophenyl)methyl cation and bis(4-alkylideneaminophenyl)methyl cation is in the red region on the long wavelength side.

Therefore, when colorimetrically quantifying this compound, the absorption wavelength is not affected by proteins in saliva. In particular, bis(4-diethylaminophenyl) methyl cation is preferable because the p++ region of maximum color development is an acidic region close to neutrality, and when it is made into a test paper, the stability of the dye is high. In this regard, bis(4-diethylaminophenyl)methanol (BEC-0) 1 ) and bis(4-dimethylaminophenyl)methanol (
Regarding BDC-O1+), the extent of dark blue (615 nm) color development with respect to changes in pl) value was investigated. Analytical Biochemistry
o-chemistry) Vol. 52, P, 127-1
42 (1973), a certain amount of an acetone solution of these compounds was added to pl+ 5.0°5.5.6.0.6
．． 5.7.0 in 100mM citrate buffer.

As a result, the maximum color development of bis(4-dimethylaminophenyl)methanol (BDC-011) was p++s.

On the other hand, bis(4-diethylaminophenyl)
Maximum color development of methanol (BFC-Kano) occurs at pH 6.0 to 6.
．． 5, which was almost in the neutral range. This color development is achieved by adding an anionic surfactant.

It will be further strengthened. Examples of anionic surfactants include higher fatty acid salts (soaps), alkanesulfonates,
Hydroxyalkanesulfonate.

Alkylbenzene sulfonate, N-alkyl sulfosuccinic acid monoamide salt, alkyl sulfate ester salt, polyoxyethylene alkyl ether sulfur 'fJ1 ester salt,
There are fatty acid monoglyceride sulfate ester salts, alkyl phosphate ester salts, and polyoxyethylene alkyl ether phosphate ester salts.

Bis(4-dialkylaminophenyl)methanol and bis(4-alkylideneaminophenyl)methanol contained in the halitosis detection reagent of the present invention are represented by lithium aluminum hydride (
It is synthesized by replacing the carbonyl group of a substituted henzphenone with LiAIIIn).

(Margin below) Here, R6 and R2 are the same as defined above.

Any compound of the present invention can be synthesized by such a synthetic method. In particular, bis(4-diethylaminophenyl)methanol (BIEC-OH) is easily obtained. Such compounds are used in the form of solutions or test strips to detect bad breath.

The above reagent can be made into a test paper by simply impregnating a carrier such as filter paper with a buffer solution containing the reagent and air-drying it. When the support is dried, the reagent solution may be concentrated in the periphery of the support surface, or if a saliva stopper is dropped at the center of the support surface, the impregnated reagent may migrate to the periphery due to capillary action, causing concentration of the reagent solution in the sample. There are cases where the measured value of bad breath components does not show the true value (window frame phenomenon). In order to suppress such phenomena, additives (window-frame phenomenon suppressants) such as methylcellulose and polyethylene glycol are added to the reagent solution. When a support is impregnated with a reagent solution containing additives, the viscosity of the liquid phase increases when the support is dried or a sample is dropped, suppressing the movement of solutes, and as a result, the window frame phenomenon described above is suppressed. .

The reagent solution used usually contains polyethylene glycol (average molecular weight 20,000) as an additive at 0.1 to 1.0% (
For example, in a citrate buffer containing (preferably 0.4%)
Bis(4-diethylaminophenyl)methanol (BE
C-OH) in acetone solution (2.4 .mu.M) is added dropwise and the color is sufficiently developed.

This reagent solution is then impregnated onto a piece of filter paper.

This is dried by freeze-drying, cut into thin pieces and pasted on a piece of white plastic to be used as a halitosis test paper for testing.

(The following is a blank space) The halitosis measuring method of the present invention is carried out using the halitosis detection reagent of the present invention in the form of a solution or a test paper.

Breath odor measurement in the form of a solution is carried out, for example, as follows: Bis(4-diethylaminophenyl)methanol (BI
An acetone solution of EC-OH) is added dropwise to an acidic buffer solution (preferably pl+ 6.0 to 6.5) to develop a dark blue color, thereby forming a halitosis detection reagent solution. A specimen is added to this halitosis detection reagent solution and mixed, and the absorbance (615 nm) of the mixed solution is measured using a colorimeter. The measured values are applied in advance to a calibration curve showing the relationship between mercaptan concentration and absorbance, and the mercaptan concentration in the sample is read.

To measure halitosis using a test paper, several liters of the sample (saliva) is poured onto the above test paper impregnated with a reagent such as bis(4-diethylaminophenyl)methanol (BEC-OB).
7! Drop it and observe the fading of the deep blue color due to the reaction between the reagent and mercaptan. Qualitative methods for determining the presence or absence of bad breath include: 1) The concentration of mercaptan contained in the saliva of a person whose breath is detected is set as a standard value, and the dark blue color of the reagent fades after a certain period of time at this concentration of mercaptan. Prepare the reagent concentrations as follows. A sample is dropped onto a test paper impregnated with the same concentration of reagent, and the presence or absence of bad breath is determined by the fading of the deep blue color after the same amount of time. Further, as a method for quantitatively determining bad breath, there is a method using a standard colorimetric table prepared by dropping a known concentration of mercaptan onto a test paper. However, there is a more accurate measurement of bad breath.

For example, the apparatus shown in FIG. 1 may be used. This halitosis measuring device 1 includes a transparent plate 3 on which a carrier 2 impregnated with a reagent is placed, a light emitting element 4 disposed below the transparent plate 3, and a light emitting element 4 disposed through a stray light prevention plate 5. It has a light receiving element 6 disposed near the light receiving element 6, and a display means 8 for numerically displaying the intensity of the reflected light from the carrier 2 detected by the light receiving element 6. In order to accurately display the intensity of this reflected light on the display means 8, the display means 8 is electrically connected to the light receiving element 6 via an amplification/measuring circuit/AD converter 7. The light emitting element 4 and the amplification/measuring circuit/A-D converter 7 are connected by a measuring switch 9.

As the light emitting element 4, for example, a light emitting diode having an emission spectrum in the vicinity of 600 to 620 nm (for example, EAA5504S manufactured by Stanley; emission spectrum 6) is used.
05 nm, Δλ30 nm, or GL-58D10 manufactured by Sharp Corporation. As the light receiving element 6.

For example, a silicon photodiode (eg, 51226-5BQ manufactured by Hamamatsu Photonics Co., Ltd.) is used as the photodetecting element 2 that is sensitive to light having a wavelength around 600 to 620 nm. The means 8 for numerically displaying the intensity of the reflected light has a built-in microprocessor, and a calibration curve representing the relationship between the change in the intensity of the reflected light and the mercaptan concentration and/or the sensory evaluation value of bad breath is previously input into its RAM. Is possible.

(Function) Using the halitosis measuring device 1 of the present invention, the main components of bad breath are measured as follows. A holder 2 impregnated with a reagent is attached to the tip of a white plastic strip 21, and a sample (saliva or standard solution for preparing a calibration curve) is dropped onto the holder 2. At the same time as the sample is dropped, a timer (not shown) is turned on, and at the same time, the support 2 is placed on the transparent plate 3 with the support 2 facing the transparent body 3. Close the light shielding cover 22.

Then, the measurement switch 9 is turned on over time to cause the light emitting element 4 to emit light. The light reflected by the carrier 2 is received by a light receiving element 6, passes through an amplification/measuring circuit/A-D converter 7, and then a display means 8 numerically displays the intensity of the reflected light.

(Example) The present invention will be described below with reference to examples.

(1) Synthesis of bis(4-diethylaminophenyl)methanol (BEC-011): 4.4'-bis(diethylamino)benzophenone (
(manufactured by Aldrich) 1.6g of dry ether 70m7
! It was dissolved in While stirring this solution under water cooling, lithium aluminum hydride (manufactured by Merck & Co., Ltd.) 0 and Ig were added little by little to react. Return the reaction solution to room temperature and heat for about 1
After stirring for an hour, disappearance of the starting material was confirmed using silica gel thin layer chromatography (using a hexane/ethyl acetate mixed solvent with a volume ratio of 5=1 as the developing solvent). A small amount of wood chips was added to the reaction solution, and the mixture was diluted with ether 3Qm6. The ether layer was dehydrated with anhydrous sodium sulfate and then filtered. When the filtrate was concentrated under a nitrogen stream, 1.5
3 g of white crystals were obtained (95% yield). When this crystal was recrystallized from a hexane-ether system and subjected to NMR analysis, it was confirmed to be bis(4-diethylaminophenyl)methanol (BIEC-OH). The melting point of the crystals was 79-80°C (literature value 78.5-79°C).

J, Ar1enL & J, Dvorak, Che
mlisty 5vazek 48 (1954)
, 1581-1582).

(2) Preparation of bad breath detection reagent solution: Bis(4-diethylaminophenyl)methanol (BE
C-OH) 7.7 mg (24 μmol) in acetone 1
Dissolved in 0 m#. 0.28m6 of this solution was 10%
100 μl of sodium dodecyl sulfate (SDS) and 10
0mM citrate buffer (pH 6,5) 9.9ml! When added dropwise to a mixed solution of
The halitosis detection reagent solution m) was obtained.

(3) Measurement: Add 100mM citrate buffer (pH 6) to the spectrometer cell.
,5)2 mj! Add 0.5 mg of the above reagent, and add 0.05 mg of the sample (saliva) with known mercaptan concentration.
ml and mixed. After 30 minutes, the absorbance of the mixture (6
15 nm) was measured. FIG. 2 shows the relationship between absorbance (615 nm) and mercaptan concentration (ppm). What is the regression equation?

(^bs) = 0.896-0.00735 X (p
pm), R=-0,992, which was an almost linear relationship.

Relative carbon dioxide bis(4-diethylaminophenyl)methanol (rl
In place of Ec-'0, 6.4 mg (24, crmol) of bis(4-dimethylaminophenyl) methanol (BDC-OH, manufactured by Wako Pure Chemical Industries, Ltd.) was used, and 100 m
Except that the pH of the M citrate buffer was 5.01.
A halitosis detection reagent was obtained in the same manner as in Example 1. About this reagent.

Absorbance (605 nm) was measured by the same method as in Example 1. Absorbance (605 nm) and mercaptan concentration (
Fig. 2 shows the relationship with ppm). What is the regression equation?

(Abs) -0,446-0,00173X (ppm
), R = -0,985.

As is clear from FIG. 2, the bis(4-diethylaminophenyl)methanol (BEC-011) contained in the halitosis detection reagent of the present invention is different from the conventional bis(4-dimethylaminophenyl)methanol (BDC-OH). Compared to
Extremely sensitive. The sensitivity ratio is 0.0073510.
00173 = 4.2 times.

Main part (1) Preparation of bad breath test paper: Bis(4-diethylaminophenyl)methanol (BEC-OH) obtained by the same method as in Example 1 7.
7+ng (24 umol) was dissolved in acetone 10+1. 2 ml of this solution was added to polyethylene glycol (PEG2000) with an average molecular weight of 20,000 as a window frame phenomenon suppressant.
0, manufactured by Wako Pure Chemical Industries, Ltd.) into 8 ml of 100 mM citrate buffer (pH 6,5), and
Add mM citrate buffer to make 10 ml. After leaving for 30 minutes.

A deep blue halitosis detection reagent (maximum wavelength 615 nm) was obtained. A filter paper (manufactured by Toyo Roshi Co., Ltd., N1131) was impregnated with the reagent and freeze-dried for 2 hours. The obtained pale blue filter paper was cut into a size of 5 mmXIQu+ and fixed to a piece of white plastic with double-sided tape to prepare a breath odor test paper.

(2) Measurement: The halitosis test paper was fixed to the carrier 2 of the halitosis measuring device shown in FIG. To this, 20 μl of a mercaptan solution of known concentration was added dropwise. Mercaptan't8? (Turn on the timer (not shown) at the same time as dropping l.

At the same time, this carrier 2 was placed on a transparent plate 3 with the side facing the transparent body 3. The light shielding cover 22 was closed, and the measurement switch 9 was turned on over time to cause the light emitting element 4 to emit light. The light reflected by the carrier 2 was received by a light receiving element 6, passed through an amplification circuit, a measuring circuit, and an A-D converter 7, and the intensity of the reflected light was displayed numerically by a display means 8.

FIG. 3 shows the relationship between elapsed time and reflected light intensity.

As is clear from FIG. 3, the intensity of reflected light increases as one hour passes after dropping the mercaptan solution. In particular, the intensity of reflected light increases significantly up to 30 seconds. Therefore,
After dropping the mercaptan solution, the change value of the reflected light intensity for 20 seconds from 10 seconds to 30 seconds is calculated as the reflected light intensity increase rate (
Δ13), and this was used as a guideline for the reflected light intensity. Vary the mercaptan concentration.

The rate of increase in reflected light intensity (Δ1.) was measured for each concentration. FIG. 4 shows the relationship between the rate of increase in reflected light intensity (Δ13) and mercaptan concentration (ppm). What is the regression equation?

Δ+i=1, I5+3.30X (ppm), R=
It was 0.988.

(3) Confirmation of the correlation between the rate of increase in reflected light intensity and the sensory evaluation of halitosis: The sensory evaluation value of halitosis based on the following criteria and the sample (saliva) corresponding to that evaluation value were dropped onto a halitosis test paper and measured. The relationship with the reflected light intensity increase rate (Δ3.) is shown in FIG.

Breath odor sensory evaluation (OR) 0: No to low odor, low degree of acceptable odor.

1: Low to medium-low odor, medium-low halitosis, mostly tolerable, some with unpleasant odor.

2: Moderate to strong bad odor, moderate and strong bad breath, mostly unpleasant odor.

3: Strong bad odor, unpleasant (strong) bad breath.

As is clear from FIG. 5, the reflected light intensity increase rate (Δ13) measured using the halitosis test paper of the present invention has a high correlation with the sensory evaluation of halitosis. Therefore, (1) the halitosis test paper of the present invention can be fully utilized for objective evaluation of halitosis (2).

Comparison I Bis(4-diethylaminophenyl)methanol (BE
C-011), bis(4-dimethylaminophenyl)methanol (BDC-OH, manufactured by Wako Pure Chemical Industries, Ltd.)
．． A halitosis test paper was prepared in the same manner as in Example 1, except that 4 mg (24 u mol) was used and p++ of 100 mM citrate buffer was set to 5.01. using this test paper.

The reflected light intensity increase rate (Δ1.
) was measured. FIG. 4 shows the relationship between the rate of increase in reflected light intensity (Δ13) and mercaptan concentration (ppm).

What is the regression equation?

Δ13=6.06+1.38X (I)pm), R
=0.994.

As is clear from FIG. 4, the bis(4-diethylaminophenyl)methanol (BEC-OH) contained in the breath test paper of the present invention is different from the conventional bis(4-dimethylaminophenyl)methanol (BDC-011). The sensitivity is significantly higher than that of The sensitivity ratio is 3.30/1.38 #2.
It is 4 times more.

When the saliva of a person with a 3 halitosis sensory rating of 2 was applied to the halitosis test paper prepared according to Main Example 2 of Fuba M, after 20 seconds, the color faded to the extent that the blue color could hardly be seen with the naked eye.

Furthermore, even when the saliva of a person with a breath odor sensory rating of 1 was applied to a similar test paper, the color fading progressed after 30 seconds or more, and blue color was hardly observed.

For this reason, the presence or absence of bad breath can be easily and quickly determined by applying saliva to the halitosis test paper of the present invention and checking with the naked eye the state of discoloration 20 to 30 seconds later.

Comparative Example 3 The fading status of the halitosis test paper containing bis(4-dimethylaminophenyl)methanol (BDC-011>) prepared in Comparative Example 2 was examined in the same manner as in Example 3. As a result, the fading state The time required was longer than 1 minute, the sensitivity was low, and complete discoloration could not be obtained.

(Effects of the Invention) According to the present invention, a halitosis detection reagent having higher sensitivity than conventional reagents can be obtained. Using this halitosis detection reagent, it is possible to easily measure mercaptan, which is the main component of bad breath, in a short period of about 30 seconds with high precision. Therefore, it becomes possible for dentists and dental hygienists to perform immediate quantitative halitosis diagnosis in the examination room, which was previously impossible, and patient guidance becomes extremely effective. Furthermore, qualitative analysis of halitosis can be easily done using halitosis test strips, so it is recommended that people who are concerned about bad breath socially
A simple method for checking bad breath can be provided.

[Brief explanation of drawings]

FIG. 1 is a schematic diagram showing one embodiment of the halitosis measuring device of the present invention, and FIG. 2 is a schematic diagram showing an embodiment of the halitosis measuring device of the present invention. Figure 3 is a graph showing the relationship between the absorbance of the reagent and the mercaptan concentration when a sample with a known concentration is added. FIG. 4 is a graph obtained by measuring the rate of increase in reflected light intensity over time using the device of the present invention, and shows the results obtained using the device and method of the present invention for the halitosis detection reagent of the present invention and the conventional halitosis detection reagent. Figure 5 shows the correlation between the rate of increase in reflected light intensity measured by the halitosis detection reagent, device, and method of the present invention and the sensory evaluation of bad breath. It is a graph. DESCRIPTION OF SYMBOLS 1...Halitosis measuring device, 2...Carrier, 3...Transparent plate. 4... Light emitting element, 5... Stray light prevention plate, 6... Light receiving element. 7... Width increaser/measuring circuit/A-D converter, 8... Display means, 9... Measurement switch, 21... Carrier holding strip, 22... Light shielding cover. that's all

Claims (1)

[Claims] 1. Bis(4-dialkylaminophenyl)methanol represented by formula I and/or bis(4-dialkylaminophenyl) methanol represented by formula II
A halitosis detection reagent containing 4-alkylideneaminophenyl)methanol. ▲There are mathematical formulas, chemical formulas, tables, etc.▼... I Here, R_1 is an alkyl group having 2 or more carbon atoms ▲There are mathematical formulas, chemical formulas, tables, etc.▼...II Here, R_2 is an alkylidene group 2, the above The halitosis detection reagent according to claim 1, wherein the bis(4-dialkylaminophenyl)methanol is bis(4-diethylaminophenyl)methanol. 3. The bad breath detection reagent according to claim 1, which is in the form of a solution. 4. The bad breath detection reagent according to claim 1, which is in the form of a test paper. 5. The bad breath detection reagent according to claim 4, which is impregnated with a window pane phenomenon suppressant. 6. The bad breath detection reagent according to claim 5, wherein the window pane phenomenon suppressant is methylcellulose or polyethylene glycol. 7, (1) A carrier impregnated with a halitosis detection reagent containing bis(4-dialkylaminophenyl)methanol represented by formula I and/or bis(4-alkylideneaminophenyl)methanol represented by formula II, The process of applying sample saliva and reacting with mercaptan, which is the main component of bad breath. , tables, etc.▼...II Here, R_2 is an alkylidene group (2) A step of irradiating the support with light having the maximum absorption wavelength of the reagent and measuring the intensity of the reflected light from the support; and (3) a step of reading the mercaptan concentration in the sample saliva by applying the measured value to a calibration curve showing the relationship between the reflected light increase rate and mercaptan concentration obtained in advance from steps (1) and (2) above. A halitosis measurement method that includes. 8. The halitosis measuring method according to claim 7, wherein the bis(4-dialkylaminophenyl)methanol is bis(4-diethylaminophenyl)methanol. 9. The method for measuring bad breath according to claim 7, wherein the carrier is in the form of a test paper. 10. The method for measuring bad breath according to claim 9, wherein the test paper-like carrier is impregnated with a window pane phenomenon suppressant. 11. The method for measuring bad breath according to claim 10, wherein the window pane phenomenon inhibitor is methylcellulose or polyethylene glycol. 12, (1) A carrier impregnated with a halitosis detection reagent containing bis(4-dialkylaminophenyl)methanol represented by formula I and/or bis(4-alkylideneaminophenyl)methanol represented by formula II; ▲There are mathematical formulas, chemical formulas, tables, etc.▼... I Here, R_1 is an alkyl group with 2 or more carbon atoms ▲There are mathematical formulas, chemical formulas, tables, etc.▼...II Here, R_2 is an alkylidene group (2) (3) a light-emitting element that emits light in the maximum absorption wavelength region of the reagent to the carrier; and (3) a light-receiving element that is sensitive to the wavelength region and receives reflected light from the carrier of the light from the light-emitting element. (4) A display means for irradiating the light from the light-emitting element onto the carrier to which the sample saliva has been applied and displaying a change in the intensity of the reflected light received by the light-receiving element. . 13. The breath odor measuring device according to claim 12, wherein the bis(4-dialkylaminophenyl)methanol is bis(4-diethylaminophenyl)methanol. 14. Claim 1, wherein the carrier is in the form of a test paper.
The breath odor measuring device according to item 2. 15. The breath odor measuring device according to claim 14, wherein the test paper-like carrier is impregnated with a window pane phenomenon suppressant. 16. The breath odor measuring device according to claim 15, wherein the window pane phenomenon suppressant is methylcellulose or polyethylene glycol. 17. Claim 12, wherein the light emitting element is a light emitting diode having a wavelength maximum around 600 to 620 nm.
The breath odor measuring device described in . 18. The breath odor measuring device according to claim 12, wherein the light receiving element is a photodiode.