JPS6155975B2 - - Google Patents
Info
- Publication number
- JPS6155975B2 JPS6155975B2 JP52096223A JP9622377A JPS6155975B2 JP S6155975 B2 JPS6155975 B2 JP S6155975B2 JP 52096223 A JP52096223 A JP 52096223A JP 9622377 A JP9622377 A JP 9622377A JP S6155975 B2 JPS6155975 B2 JP S6155975B2
- Authority
- JP
- Japan
- Prior art keywords
- medium
- sperm
- column
- convection
- spermatozoa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000004027 cell Anatomy 0.000 claims description 78
- 238000000034 method Methods 0.000 claims description 50
- 238000001556 precipitation Methods 0.000 claims description 49
- 238000001962 electrophoresis Methods 0.000 claims description 22
- 230000004087 circulation Effects 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 16
- 210000000582 semen Anatomy 0.000 claims description 16
- 238000004062 sedimentation Methods 0.000 claims description 14
- 210000002593 Y chromosome Anatomy 0.000 claims description 9
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 239000004471 Glycine Substances 0.000 claims description 4
- 210000001766 X chromosome Anatomy 0.000 claims description 4
- 235000013345 egg yolk Nutrition 0.000 claims description 4
- 210000002969 egg yolk Anatomy 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 claims description 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 description 52
- 238000000926 separation method Methods 0.000 description 24
- 241000283690 Bos taurus Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 241000282412 Homo Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 230000035699 permeability Effects 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 241000283086 Equidae Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 241000288906 Primates Species 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 239000012737 fresh medium Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000009182 swimming Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 208000013403 hyperactivity Diseases 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 229960000901 mepacrine Drugs 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 210000003739 neck Anatomy 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- GPKJTRJOBQGKQK-UHFFFAOYSA-N quinacrine Chemical compound C1=C(OC)C=C2C(NC(C)CCCN(CC)CC)=C(C=CC(Cl)=C3)C3=NC2=C1 GPKJTRJOBQGKQK-UHFFFAOYSA-N 0.000 description 2
- UKOBAUFLOGFCMV-UHFFFAOYSA-N quinacrine mustard Chemical compound C1=C(Cl)C=CC2=C(NC(C)CCCN(CCCl)CCCl)C3=CC(OC)=CC=C3N=C21 UKOBAUFLOGFCMV-UHFFFAOYSA-N 0.000 description 2
- 241000030939 Bubalus bubalis Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000219173 Carica Species 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- YZCKVEUIGOORGS-UHFFFAOYSA-N Hydrogen atom Chemical compound [H] YZCKVEUIGOORGS-UHFFFAOYSA-N 0.000 description 1
- 208000017924 Klinefelter Syndrome Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 208000026928 Turner syndrome Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 231100000690 decreased sperm viability Toxicity 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 208000012978 nondisjunction Diseases 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000009589 serological test Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Cell Biology (AREA)
- Reproductive Health (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Artificial Fish Reefs (AREA)
- Farming Of Fish And Shellfish (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Techniques For Improving Reliability Of Storages (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/734,243 US4092229A (en) | 1975-12-17 | 1976-10-20 | Thermal convection counter streaming sedimentation and forced convection galvanization method for controlling the sex of mammalian offspring |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5356381A JPS5356381A (en) | 1978-05-22 |
| JPS6155975B2 true JPS6155975B2 (OSRAM) | 1986-11-29 |
Family
ID=24950870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9622377A Granted JPS5356381A (en) | 1976-10-20 | 1977-08-12 | Method and apparatus for separating sperm cell from seminal fluid |
Country Status (12)
| Country | Link |
|---|---|
| JP (1) | JPS5356381A (OSRAM) |
| AR (1) | AR215016A1 (OSRAM) |
| AU (1) | AU518797B2 (OSRAM) |
| BE (1) | BE857726A (OSRAM) |
| BR (1) | BR7705336A (OSRAM) |
| CA (1) | CA1088025A (OSRAM) |
| FR (1) | FR2368299A1 (OSRAM) |
| MX (1) | MX148863A (OSRAM) |
| NL (1) | NL7708738A (OSRAM) |
| NO (1) | NO147473C (OSRAM) |
| NZ (1) | NZ184883A (OSRAM) |
| PH (1) | PH15407A (OSRAM) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2511445B2 (ja) * | 1987-03-06 | 1996-06-26 | 日清製粉株式会社 | 豚のｙ―精子の検定方法 |
| US6673225B1 (en) | 1998-05-29 | 2004-01-06 | Industrial Research Limited | Method and apparatus for concentrating and/or positioning particles or cells |
| AU2013234096B2 (en) * | 2012-03-16 | 2018-08-09 | Fertility Innovations Limited | Processing of sperm cells |
| AU2013309137B2 (en) | 2012-08-29 | 2016-11-10 | Inguran, Llc | Magnetic removal or identification of damaged or compromised cells or cellular structures |
| US10379026B2 (en) | 2012-08-29 | 2019-08-13 | Inguran, Llc | Cell processing using magnetic particles |
-
1977
- 1977-07-19 CA CA283,060A patent/CA1088025A/en not_active Expired
- 1977-08-03 AU AU27565/77A patent/AU518797B2/en not_active Expired
- 1977-08-08 NL NL7708738A patent/NL7708738A/xx not_active Application Discontinuation
- 1977-08-10 AR AR268746A patent/AR215016A1/es active
- 1977-08-10 NO NO772814A patent/NO147473C/no unknown
- 1977-08-10 NZ NZ184883A patent/NZ184883A/xx unknown
- 1977-08-11 FR FR7724795A patent/FR2368299A1/fr active Granted
- 1977-08-12 JP JP9622377A patent/JPS5356381A/ja active Granted
- 1977-08-12 BR BR7705336A patent/BR7705336A/pt unknown
- 1977-08-12 PH PH20113A patent/PH15407A/en unknown
- 1977-08-12 MX MX170215A patent/MX148863A/es unknown
- 1977-08-12 BE BE2056155A patent/BE857726A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BE857726A (fr) | 1977-12-01 |
| NL7708738A (nl) | 1978-04-24 |
| NZ184883A (en) | 1979-08-31 |
| AU2756577A (en) | 1979-02-08 |
| MX148863A (es) | 1983-06-28 |
| PH15407A (en) | 1982-12-24 |
| BR7705336A (pt) | 1979-03-13 |
| NO772814L (no) | 1978-04-21 |
| CA1088025A (en) | 1980-10-21 |
| FR2368299B3 (OSRAM) | 1980-06-20 |
| AR215016A1 (es) | 1979-08-31 |
| NO147473C (no) | 1983-04-20 |
| FR2368299A1 (fr) | 1978-05-19 |
| JPS5356381A (en) | 1978-05-22 |
| NO147473B (no) | 1983-01-10 |
| AU518797B2 (en) | 1981-10-22 |
Similar Documents
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