JPS6155956B2 - - Google Patents
Info
- Publication number
- JPS6155956B2 JPS6155956B2 JP3355679A JP3355679A JPS6155956B2 JP S6155956 B2 JPS6155956 B2 JP S6155956B2 JP 3355679 A JP3355679 A JP 3355679A JP 3355679 A JP3355679 A JP 3355679A JP S6155956 B2 JPS6155956 B2 JP S6155956B2
- Authority
- JP
- Japan
- Prior art keywords
- lysine
- leucine
- corynebacterium
- valine
- mutant strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 10
- 235000019766 L-Lysine Nutrition 0.000 claims description 9
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 9
- 239000004472 Lysine Substances 0.000 claims description 9
- 229960003136 leucine Drugs 0.000 claims description 6
- 241000186216 Corynebacterium Species 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 229960004295 valine Drugs 0.000 claims description 5
- 239000004395 L-leucine Substances 0.000 claims description 4
- 235000019454 L-leucine Nutrition 0.000 claims description 4
- 241000186146 Brevibacterium Species 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 claims 1
- 241000894006 Bacteria Species 0.000 description 3
- 241000186226 Corynebacterium glutamicum Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 1
- UKIDUMMXBQMTKO-UHFFFAOYSA-N 1-methyl-1-nitro-2-nitrosoguanidine Chemical compound [O-][N+](=O)N(C)C(=N)NN=O UKIDUMMXBQMTKO-UHFFFAOYSA-N 0.000 description 1
- YBJHBAHKTGYVGT-ZXFLCMHBSA-N 5-[(3ar,4r,6as)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid Chemical compound N1C(=O)N[C@H]2[C@@H](CCCCC(=O)O)SC[C@H]21 YBJHBAHKTGYVGT-ZXFLCMHBSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241001485655 Corynebacterium glutamicum ATCC 13032 Species 0.000 description 1
- 241000807905 Corynebacterium glutamicum ATCC 14067 Species 0.000 description 1
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 1
- GHSJKUNUIHUPDF-BYPYZUCNSA-N L-thialysine Chemical compound NCCSC[C@H](N)C(O)=O GHSJKUNUIHUPDF-BYPYZUCNSA-N 0.000 description 1
- 241001467578 Microbacterium Species 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960003767 alanine Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229960002989 glutamic acid Drugs 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Description
【発明の詳細な説明】
この発明は発酵法によるＬ―リジンの製法に関
する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing L-lysine by fermentation.
本発明者らは、より効率の高いＬ―リジン生産
菌を誘導すべく研究した結果、従来知られている
ブレビバクテリウム属、コリネバクテリウム属の
Ｌ―リジン生産菌にＬ―リジン又はＬ―ロイシン
を唯一の窒素源として親株以上の生育度を示すよ
うな変異株が、従来のＬ―リジン生産菌より高い
収率でＬ―リジンを生産することを見い出した。 As a result of research aimed at inducing more efficient L-lysine producing bacteria, the present inventors found that L-lysine or L- We have discovered that a mutant strain that uses leucine as its sole nitrogen source and exhibits a higher growth rate than the parent strain produces L-lysine at a higher yield than conventional L-lysine producing bacteria.
この発明は、この知見に基づいて完成されるに
到つたのである。 This invention was completed based on this knowledge.
本発明において用いられる微生物は、ブレビバ
クテリウム属、またはコリネバクテリウム属に属
し、ホモセリン要求性，Ｓ―（２―アミノエチ
ル）―Ｌ―システイン（以下AECと記す）耐
性、その他のＬ―リジン生産性を有するために必
要な性質のほか、Ｌ―バリン又はＬ―ロイシンを
唯一の窒素として親株以上の生育度を示す変異株
即ち、Ｌ―バリン又はＬ―ロイシンの分解活性が
強化された変異株である。 The microorganisms used in the present invention belong to the genus Brevibacterium or Corynebacterium, and are homoserine auxotrophic, S-(2-aminoethyl)-L-cysteine (hereinafter referred to as AEC) resistant, and other L-lysine auxotrophs. In addition to the properties necessary for productivity, a mutant strain that uses L-valine or L-leucine as the only nitrogen and exhibits higher growth than the parent strain, i.e., a mutation with enhanced L-valine or L-leucine degrading activity. It is a stock.
本発明の変異株を誘導する際に用いられる親株
は、従来Ｌ―グルタミン酸生産菌として知られて
いるブレビバクテリウム属又はコリネバクテリウ
ム属に属する微生物であり、例えば、ブレビバク
テリウム・フラバムATCC 14067，ブレビバクテ
リウム・ラクトフエルメンタム ATCC13869，
コリネバクテリウム・グルタミカム ATCC
13032，コリネバクテリウム・アセトアシドフイ
ラムATCC 13870，コリネバクテリウム・アセト
グルタミカム ATCC 15806，ミクロバクテリウ
ム・アンモニアフイラムATCC 15354などがあ
る。 The parent strain used to induce the mutant strain of the present invention is a microorganism belonging to the genus Brevibacterium or Corynebacterium, which is conventionally known as an L-glutamic acid producing bacterium, such as Brevibacterium flavum ATCC 14067. , Brevibacterium lactofermentum ATCC13869,
Corynebacterium glutamicum ATCC
13032, Corynebacterium acetoacidophyllum ATCC 13870, Corynebacterium acetoglutamicum ATCC 15806, and Microbacterium ammoniaphyllum ATCC 15354.
以下に本発明の変異株について、その具体的誘
導法の１例と、得られた菌株についてＬ―バリン
又はＬ―ロイシンを唯一の窒素源とした培地に於
ける生育度を示す実験例を示す。 Below, we will show one example of a specific induction method for the mutant strain of the present invention, and an experimental example showing the growth rate of the obtained strain in a medium with L-valine or L-leucine as the sole nitrogen source. .
実験例
ブレビバクテリウム・ラクトフエルメンタム
AJ 3990（FERM―Ｐ 3387）（AECr，ML（γ
―メチルリジン）r，Ala-）を、250μｇ／mlのＮ―
メチル―Ｎ―ニトロ―N′―ニトロソグアニジン
にて30℃で30分処理し、以下に示す最小培地に唯
一の窒素源としてバリン又はロイシン400mg／dl
添加した培地で生育したコロニーを採取した。Experimental example Brevibacterium lactofermentum
AJ 3990 (FERM-P 3387) (AEC r , ML (γ
- Methylysine) r , Ala - ), 250 μg/ml N-
Treatment with methyl-N-nitro-N'-nitrosoguanidine for 30 minutes at 30°C and 400 mg/dl of valine or leucine as the sole nitrogen source in the following minimal medium:
Colonies that grew on the added medium were collected.
最小培地組成：
グルコーズ 2.0ｇ／dl
酢酸ナトリウム 0.1ｇ／dl
KH2PO4 0.1ｇ／dl
K2HPO4 0.3ｇ／dl
MgSO4・7H2O 1.0mg／dl
FeSO4・7H2O 1.0mg／dl
MnSO4・7H2O 1.0mg／dl
Ｌ―アラニン 30 mg／dl
ニコチン酸アミド 0.5mg／dl
ビオチン 50μｇ／
サイアミン・塩酸塩 100μｇ／
PH 7.2
分離用培地：
酵母エキス 1.0ｇ／dl
「ポリペプトン」 1.0ｇ／dl
NaC1 0.5ｇ／dl
グルコース 0.2ｇ／dl
寒 天 2.0ｇ／dl
PH 7.2
この様にして得られた変異株の内、Ｌ―リジン
生産能のすぐれた変異株として
AJ 11324（FERM―Ｐ 4855）（AEC〓―MLr・
Al・Valdog Minimum medium composition: Glucose 2.0g/dl Sodium acetate 0.1g/dl KH 2 PO 4 0.1g/dl K 2 HPO 4 0.3g/dl MgSO 4・7H 2 O 1.0mg/dl FeSO 4・7H 2 O 1.0mg/ dl MnSO 4・7H 2 O 1.0mg/dl L-Alanine 30 mg/dl Nicotinamide 0.5mg/dl Biotin 50μg/ Thiamine Hydrochloride 100μg/PH 7.2 Isolation medium: Yeast extract 1.0g/dl "Polypeptone" 1.0 g/dl NaC1 0.5g/dl Glucose 0.2g/dl Agar 2.0g/dl PH 7.2 Among the mutant strains obtained in this way, AJ 11324 (FERM-P) is a mutant strain with excellent L-lysine production ability. 4855) (AEC〓―ML r・
Al・Val dog
Claims (1)
ウム属に属し、Ｌ―バリン又はＬ―ロイシンを唯
一の窒素源として親株以上の生育度を示し、かつ
Ｌ―リジン生産能を有する変異株を培養し、生成
蓄積したＬ―リジンを採取することを特徴とする
発酵法によるＬ―リジンの製造法。1. Cultivate a mutant strain belonging to the genus Brevibacterium or Corynebacterium that exhibits a growth rate higher than that of the parent strain using L-valine or L-leucine as the sole nitrogen source and has the ability to produce L-lysine, and produce and accumulate A method for producing L-lysine by a fermentation method, which comprises collecting L-lysine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3355679A JPS55124496A (en) | 1979-03-22 | 1979-03-22 | Production of l-lysin through fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3355679A JPS55124496A (en) | 1979-03-22 | 1979-03-22 | Production of l-lysin through fermentation process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS55124496A JPS55124496A (en) | 1980-09-25 |
| JPS6155956B2 true JPS6155956B2 (en) | 1986-11-29 |
Family
ID=12389820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3355679A Granted JPS55124496A (en) | 1979-03-22 | 1979-03-22 | Production of l-lysin through fermentation process |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55124496A (en) |
-
1979
- 1979-03-22 JP JP3355679A patent/JPS55124496A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS55124496A (en) | 1980-09-25 |
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