JPS61291525A - Food for preventing allergy and adult disease and produced from rengyo and/or related species thereof - Google Patents

Abstract

PURPOSE:To obtain a food for preventing allegy and adult diseases, capable of preventing allergy and adult diseases such as thrembosis at the same time, and takable easily as a food, by using RENGYO (Forsythia supensa Vahl) or its related species as an essential component. CONSTITUTION:The objective food for preventing allergy and adult diseases can be produced by using RENGYO or its related species as an essential component. Preferably, the dried powder, extract or concentrated extract of the fruit of RENGYO or the purified product of formula I or formula II is used in the form of cake, refreshing drink, staple food, powder, etc. The simultaneous effective prevention of allergy and adult diseases such as thrombosis, which could not be attained heretofore, can be carried out by the use of the present food.

Description

Detailed Description of the Invention (Field of Industrial Application) The present invention relates to a food for preventing allergies and adult diseases made from Forsythia and/or its related species, and its purpose is to
To provide an allergy and adult disease preventive food made from forsythia and/or its closely related species, which can prevent allergies and adult diseases at the same time.

(Prior art and its drawbacks) In general, an allergic reaction is when a foreign protein, etc. is taken into a living body and an antigen-antibody reaction occurs, which is a biological reaction. 5R5-A and the like are released, and this is understood to be an in vivo reaction that damages tissues.

Therefore, in order to suppress allergic reactions, it is necessary to use 5RS-A, a precursor of 5RS-A, which is one of the causes of allergic reactions.
-HETH production can be inhibited, and this 5-HETE
is an arachidonic acid glue poxygenase metabolite, so 5
-Those that specifically inhibit HETE are considered to have high anti-allergic effects.

In addition, thrombosis, a type of adult disease, is caused by damage to the vascular endothelium.
A condition in which platelets aggregate in blood vessels and blood clots due to inflammation, changes in blood flow, restoration of blood coagulation ability, etc.

This symptom is caused by TXBz, a metabolite of arachidonic acid,
This is increased by the increase in HIT, and this TX
B2, HHT is a metabolite of the arachidonic acid produced by the platelet enzyme cyclooxygenase.

In addition, PGI is activated by the leukocyte enzyme cyclooxygenase.
2 will be produced. This PGI2 is TXA2 (TXB2)
It has an antagonistic effect on preventing thrombosis and inhibiting platelet aggregation.

In other words, arachidonic acid has two metabolic systems, one by the enzyme cyclooxygenase and the other by the enzyme lipoxygenase, and these metabolic systems differ depending on leukocytes and platelets, and if the former metabolic system by platelet cyclooxygenase functions, it will lead to thrombosis. Also, allergic reactions occur when the metabolic system by leukocyte lipoxygenase functions.

Furthermore, if the metabolic system by lipoxygenase is suppressed and the metabolic system by platelet cyclooxygenase is activated, thrombosis tends to occur.

Moreover, there are only two metabolic systems for arachidonic acid, and there are no medicines that can simultaneously suppress thrombosis and allergies.Not only is there no food available, but as mentioned above, if you prevent thrombosis too much, allergies will develop. There was a truly difficult and contradictory problem in that over-prevention of allergic diseases could lead to the development of thrombotic syndrome.

Therefore, in recent years, as the number of obese adults and children due to high carbohydrate and high fat diets has been increasing, the incidence of thrombotic syndromes such as allergies and arteriosclerosis due to obesity is increasing. There are some people who exhibit symptoms or even both symptoms, and preventive foods that eliminate the harmful effects of obesity in people (adults and children) before they develop allergies and adult diseases (thrombotic syndrome, etc.) are needed to treat obesity. There has been an urgent need among people and industry to create a food product that preventively avoids the harmful effects of obesity.

(Progress of the Invention) Therefore, the inventor conducted intensive research to eliminate all of the above-mentioned conventional drawbacks, and surprisingly, an allergy and adult disease preventive food made from Forsythia and/or its related species was discovered. It was found that allergic reactions and adult diseases (such as various thromboses) can be inhibited simultaneously.

As a result, it was discovered that if a food is prepared using Forsythia and/or its related species as a raw material, it can be easily consumed as a food, and it is highly effective in preventing allergies and adult diseases (such as various thrombosis). reached.

(Solution Means) That is, this invention solves the problem of Forsythia 5.
The present invention relates to an allergy and adult disease preventive food made from forsythia and/or its related species as an essential ingredient.

(Effect of the invention) Forsythia used in this invention is Oleaceae (OL).
eaceae), and its scientific name is Forsythia 5upensa V.
ahl).

In this invention, this forsyth (Forsyth)
ia 5upensa Vahl), such as Forsythia viridissm
Closely related species of deciduous shrubs of the Oleaceae family, such as A Lindl), can be used.

In this invention, the whole part of the forsythia is used, preferably the dried mature fruit, that is, the dried fruit.

In this invention, in order to obtain a preventive food for allergies and adult diseases from all parts of Forsythia, preferably dried fruit, dry powder (lyophilized product, hot air dried product, natural dried product, extracted dried product) or extract is used. do it.

For example, in order to obtain an extract, first extraction is performed from dried fruits of Forsythia or Forsythia.

The extraction method is either hot water extraction or extraction with oxygen-containing solvents, alcohols, ethers, ketones, esters, etc.

In this invention, a hot water extract from the dried fruit of Forsythia or Forsythia (hereinafter referred to as Forsythia etc.) is obtained as follows.

That is, cut the dried fruit of Forsythia and cut this cut into 1~
10hrs preferably 5hrs 60-80℃ preferably 7
The dried fruit is immersed in hot water at 0°C to extract the hot water-soluble components in the dried fruit cut into the hot water, and then filtered to obtain a filtrate containing the hot water extract.The filtrate is concentrated and further sprayed. Dry to obtain a hot water extract in powder form.

Depending on the case, a concentrated extract may be used.

Further, in the present invention, an extract using an oxygen-containing organic solvent from dried fruits such as forsythia can be obtained as follows.

That is, an oxygen-containing organic solvent, preferably ethanol, and in some cases acetone is added to dried fruits such as forsythia, and the mixture is heated at 20 to 60°C, preferably 40°C.
Leave for 4 hours, preferably 4 hours. In this case, room temperature (2
5°C), it may be left overnight.

However, after the solvent-soluble components in dried fruits such as forsythia are extracted into a solvent, filtration is performed, and the filtrate is concentrated and further spray-dried to obtain a solvent extract in the form of a powder. The solvent extract may remain in the form of a concentrated extract.

If necessary, the hot water extract or solvent extract obtained as described above is purified by column chromatography.

Through this purification, the purified product (I) obtained from the extract of Forsythia
side ] (the following formula (1)), and the purified product (If) obtained from the extract of the dried fruit of Psyllium sinensis is acteoside [Acteoside] (the following formula (2))
It is.

Allergy and adult disease prevention food made from forsythia powder, extract powder, concentrated extract, purified product (I), (■) of forsythia and/or its related species obtained as above. To obtain it, it can be carried out according to conventional methods, that is, it can be prepared into confectionery, soft drinks, staple foods, powders, and granules, and fillers, flavoring agents, sweeteners, and excipients may be added as necessary. You may add additives such as.

In addition, the allergy and adult disease preventive food according to the present invention usually contains 50 ml of the above-mentioned forsythia powder per adult.
It may be prepared so that it can be eaten from 0 to 10,000 square meters, preferably about 1,000 square meters.

(Effects of the Invention) As described above, forsythia and/or forsythia containing extracts extracted from forsythia and/or its related species are essential ingredients.
Foods for preventing allergies and adult diseases made from this closely related species are not only non-medicinal, so they can be easily used at home, and can also be used to effectively treat both allergies and adult diseases such as thrombosis, which was previously impossible. It has the effect of being able to prevent this.

Examples of the allergy and adult disease preventive food made from forsythia and/or its related species according to the present invention are as follows:
The effects of this invention will be made clearer by describing experimental examples.

(Example 1) Dried forsythia fruit was cut, and 1000 g of the cut material was cut.
The dried fruit was immersed in hot water 31 at 70°C for 5 hours to extract the hot water-soluble components in the dried fruit, and then filtered to obtain a filtrate containing the hot water extract, and the filtrate was concentrated, and further A hot water extract was obtained in powder form by spray drying.

The powder of this hot water extract was purified by column chromatography to obtain purified product (I) from the hot water extract of Forsythia
Purified product (n) actioside was obtained from extracts of , Forsitiaside, and Cinnamon.

500 μg of fructose was added to each of the purified products (1) and (n) to prepare a powder of 3 g per package, which was used as a food for preventing allergies and adult diseases.

(Example 2) Dried fruits of Psyllium were cut into pieces, and 50% of the cut pieces were
Add 51 ethanol to 0g and heat at 40°C.
After leaving for hours to extract the ethanol-soluble components in dried fruits such as forsythia into etaler, filtration is performed, the filtrate is extracted, and the fruit is further spray-dried to obtain an ethanol extract in the form of a powder extract.

This powder extract was purified by column chromatography to obtain purified product (I) forsitiaside from the ethanol extract of Forsythia, and purified product (n) actioside from the ethanol extract of Forsythia.

Fructose was added to 500 mg of each of the purified products (1) and (I[) to prepare a powder of 3 g per package, which was used as a food for preventing allergies and adult diseases.

(Test Example 1) Wistar male rafts were used, and the rat intraperitoneal polynuclear leukocytes were washed with HEPS-saline buffer (PH7,4), suspended in the same buffer, and subjected to ultrasound treatment. was used as an enzyme solution for arachidonic acid metabolism. This platelet homocynate and forsythiaside (For-sy-thi)
a 5ide) and acteoside (Acteosid
e) with various concentrations shown in the following table.
Incubate at 7°C for 5 minutes, then add [1-C] arachidonic acid (0
, 05 μci) and incubated for 5 minutes.

The reaction was stopped with formic acid (pH 3), and arachidonic acid metabolites were extracted with ethyl acetate and subjected to silica gel thin layer chromatography (
It was separated and quantified using TL (3) (developing solution: ethyl acetate:
2.2.4-Trimethylpentane:acetic acid:water=100
:50:20:100. V/V, upper layer, TLC is me/L
/ri574B).

Radioactive substances were detected by autoradiography, the spots were cut out, and radioactivity was quantified using a liquid scintillation counter.

The results are summarized in Tables 1 to 4.

(Leaving space below) Table 1 *The unit of forsithiaside is 1 mol x 10 millet index ※Comparative example at concentration 0 Table 2 *The unit of actioside is 1 mol x 10 millet index ※Comparative example of concentration O Table 3 *The unit of forsitiaside is the millet index of 1 mole x 10 ※※Comparison example of concentration O Table 4 *The unit of actioside is the millet index of 1 mole x 10**
Comparative Example of Concentration O As is clear from the above results, forsythiaside or acteoside, which is purified extract from Forsythia forsythia or Forsythia forsythia, inhibits the production of 5-HETE and also inhibits the production of 6-Keto. -PGFI cx (PC
In other words, health foods containing extracts extracted from Forsythia or Forsythia as an essential ingredient are effective in preventing allergies and adult diseases (
It can be seen that it has an excellent effect on the treatment of various types of thrombosis, etc.).

(Test Example 2) Twenty obese men and women were divided into two groups, and the first group was given the powder of Example 1, and the second group was given one packet of the powder of Example 2 in the morning and evening. We looked at the degree of blood pressure drop and allergic symptoms (contact allergy).

Contact allergy was determined using the Ba-ch test against irritating fragrances.

The conditions before the test are shown in Table 5, and the results after 4 weeks are shown in Table 6.

Table 5 Table 6

Claims (3)

[Claims] (1) Forsythia supens
An allergy and adult disease preventive food made from forsythia and/or its related species as an essential ingredient. (2) Forsythia supens
a Vahl) and/or related species in its dried form and/or
The allergy and adult disease preventive food made from forsythia and/or its closely related species according to claim (1), characterized in that it is a forsythia or an extract thereof. (3) Forsythia supe
Claims (1) or (2), characterized in that the extract extracted from the following formula (I) and/or (II) Allergy and adult disease preventive food made from forsythia and/or its related species. ▲There are mathematical formulas, chemical formulas, tables, etc.▼...(I) ▲There are mathematical formulas, chemical formulas, tables, etc.▼...(II)