JPS61284660A - Test body - Google Patents

Abstract

PURPOSE:To make easy and exact detection or measurement by providing high water absorptive parts to the circumference of plural detection regions consisting of separate and different reagents on a base. CONSTITUTION:The separate and different reagents or reagent compsns. are provided on the base 2. The high water absorptive parts 6 are provided at spaced intervals to the circumference of the regions 3-5. The non-water absorptive base is consequently interposed between the regions 3-5 and the parts 6. Even if a test body 1 is immersed into a liquid to be inspected, the liquid to be inspected is distributed between the regions 3-5 and the parts 6 and the reagents from the regions 3-5 are absorbed in the parts 6 so as to flow out to the other detection regions without contaminating said regions. The liquid to be tested is also absorbed in the parts 6 without remaining in the detection regions and therefore the change of the color tones in the respective detection regions is exactly judged and the detection or measurement is made exact.

Description

DETAILED DESCRIPTION OF THE INVENTION Technical Field of the Invention The present invention relates to a test specimen that can easily and accurately detect various components in a test fluid, particularly glucose, protein, occult blood, urobilinogen in a body fluid, or the pH of a body fluid. This invention relates to a test specimen that can be detected simultaneously and easily and accurately.

Technical background of the invention and its problems When discovering, diagnosing, and treating diseases, it is extremely difficult to easily and quickly know the presence or absence and amount of various components contained in body fluids such as blood, lymph, and urine. It's important.

For example, quickly and easily knowing the amount of glucose in body fluids such as urine or blood is essential for early detection, diagnosis, and management of diabetes.

In addition, quickly and easily knowing the amount of protein in body fluids, especially urine, plays a major role in early detection, diagnosis, and treatment of kidney diseases. Furthermore, accurately knowing the pl'I of body fluids, especially urine, helps to accurately know whether or not proteins exist in body fluids, as well as the possibility of bacterial beds that cause nephritis, cystitis, etc. It helps in confirming the gender. Furthermore, knowing the presence or absence of occult blood in body fluids, especially urine, plays a major role in the early detection, diagnosis, and treatment of kidney disorders, and knowing the amount of urobilinogen in body fluids, especially urine, is useful for diagnosing liver function. It plays a big role.

It is extremely important to easily and quickly detect glucose, protein, occult blood, urobilinogen, or pH of body fluids in body fluids, especially urine. Bonded test pieces have been used.

This test piece had the advantage that it was easy to operate and that judgments could be made in a short time.

However, there are some drawbacks, such as the impregnating liquid being unstable and the manufacturing process being complicated, discoloration due to exposure to the atmosphere during storage, low sensitivity and a long time required for color development, etc. depending on the target to be detected. There were various difficulties. In addition, test pieces are prepared for each type of test, which has the disadvantage that it takes time and effort to conduct each type of test.

The present inventor has previously developed a test specimen that can solve these difficulties and conduct various tests easily and quickly, and simultaneously, and has filed a patent application (%Gan Sho 39-, No. 337G7).

This ink composition is prepared by dissolving or dispersing reagent compositions for detecting various components in a solvent, and printing or coating this on a support at certain intervals.

More specifically, this test specimen is characterized by having an area on a support coated with at least seven ink compositions (al to (c)) having the following compositions.

(a) An ink composition for detecting glucose, in which a reagent composition comprising a sugar oxidase, a peroxidase, an oxidizable indicator, a binder, and a stabilizer is dissolved or dispersed in a non-aqueous solvent; (b) an ink composition for detecting protein error; (e) a pH indicator; PI (measurement ink composition) in which a reagent composition consisting of a high-grade ammonium salt or amine salt, a binder, and a water-absorbing powder is dissolved or dispersed in a solvent.

In a preferred specific embodiment, this test specimen includes a glucose detection area coated with the ink composition for glucose detection, a protein detection area coated with the ink composition for protein detection, and a pH measurement area on a support. a pH measurement area coated with an ink composition for detecting urobilinogen, and, depending on the case, a urobilinogen detection area coated with an ink composition for urobilinogen detection or an occult blood detection area coated with an ink composition for occult blood detection. have

To prepare this test specimen, the ink composition for glucose detection is coated on a support made of paper, synthetic paper, nonwoven fabric, synthetic resin film, etc., using a coating method such as roll coach ink, spray coating, or silk screen printing method. Glucose detection areas are formed by coating or printing by a printing method such as , intaglio printing or gravure printing, and protein detection areas and pH measurement areas are similarly formed at regular intervals.

When the test specimen prepared in this way is immersed in urine, for example, if glucose or protein is present in the urine, the reagents in the respective detection regions will change color, allowing rapid detection of each.
Similarly, urine pH can be easily measured. Therefore, when using this test sample, glucose, protein, and pH in urine
It can be measured easily and quickly at the same time, and is extremely effective.

In this way, when forming multiple detection areas using different reagents on one support, multiple detection or measurement operations can be performed by using one support and immersing it in urine once. Although it is efficient because it can be carried out simultaneously, on the other hand, a small amount of the detection reagent in one area flows into other detection areas along with urine and contaminates the detection reagent, inhibiting the coloring reaction of the contaminated detection reagent. This may cause errors in color tone.

[Object of the invention and its outline]

Thus, in view of the above circumstances, the present invention provides a test specimen in which a plurality of detection regions made using different reagents are formed on one support, in which each detection region prevents reagents from other detection regions from flowing out. The object of the present invention is to provide a test specimen in which the coloring reactions of the respective detection reagents proceed without any inhibition, and each detection or measurement can be carried out easily and accurately at the same time. It is something to do.

Therefore, the present invention provides a test specimen characterized in that a plurality of detection regions each made of a different reagent are provided on a support, and a highly water-absorbing region is provided around each detection region. be.

[Detailed description of the invention]

The present invention will be explained in detail with reference to FIG. 1, which shows one embodiment of the present invention. Three detection regions 3, p, and r formed by Here, each of the regions is provided in a striped manner, forming a band having a constant width from the upper end to the lower end, and spaced from each other at regular intervals.

Typically, the first detection area 3 is a glucose detection area, the second detection area 3 is a protein detection area, and the third detection area 3 is a protein detection area.
H measurement area.

In the present invention, a highly water-absorbing region 6 is provided around each detection region 3, 5, and 5.
...is established. That is, the highly water-absorbing portions 6 are provided in the form of bands or stripes of a constant width between adjacent detection regions, on the left side of the first detection region 3, and on the right side of the third detection region. In this case, a certain distance is provided between each detection area and each high water absorption part so that they do not come into direct contact with each other.

A plurality of upper stop square detection areas 13, /41, /S are provided,
It is also possible to surround the four sides with super absorbent parts/6. In this case, each detection area is provided at a constant interval, and a constant interval is also provided between a highly water-absorbing part surrounding a certain detection area and a highly water-absorbing part surrounding an adjacent detection area.

The support used here is preferably made of a non-water-absorbing material such as various synthetic resin sheets, and the super-absorbent part is made of a super-absorbent polymer that has the ability to absorb at least 10 times its own weight of pure water. Preferably, it is made from highly absorbent materials such as. As mentioned above, the detection areas 3, a, S and the high water absorption part 6
If a certain distance is provided between the detection area and the superabsorbent area, a non-water-absorbing support will be interposed in between, so even if the specimen is immersed in urine, the urine will be distributed between the detection area and the highly absorbent area. and thus prevent the reagent from flowing out from the detection area.

If there is no non-water-absorbent material intervening between the detection area and the super-absorbent member and they are in direct contact with each other, the detection reagent will be immersed in the sample liquid such as urine between the detection area and the super-absorbent member. Movement occurs;
Contamination of adjacent detection areas thus occurs. On the other hand, if there is no highly absorbent portion and only non-water-absorbing materials are present on both sides of the detection region, absorption of the test fluid (body fluid) remaining in the detection region is small.

This affects color performance, especially when detecting glucose.

As described above, the presence of the non-water-absorbing material serves to appropriately distribute the sample liquid adhering to the test specimen between the detection region and the superabsorbent member and to prevent the reagent from flowing out from the detection region.

The ink composition for forming the super absorbent portion 6 includes a water absorbent powder such as cellulose, starch, super absorbent gel, a hydrophilic or hydrophobic resin, a binder, and an inorganic filler to make the film porous. It contains additives and solvents such as.

The superabsorbent gel used here is polyvinyl alcohol (P
VA), is a crosslinked or graft copolymer mainly composed of acrylate, acrylonitrile, starch, etc., and as a commercially available super absorbent gel, Sumikagel SP rso (Sumitomo Chemical Co., Ltd.), which has a water absorption capacity of 6θ0 times, is a IM-100 (commercially available PVA-acrylate block copolymer), Sunwet (with a water absorption capacity of 7000 times)
0 (acrylic acid grafted starch commercially available from Sanyo Kasei ■), KI gel (pv
reaction products with human-cyclic acid anhydrides), etc.

〔Effect of the invention〕

According to the present invention, a plurality of detection regions each made of a different detection reagent are provided on a support, and a highly water-absorbing region is provided around the detection region.Therefore, when immersed in a test liquid such as urine, detection A small amount of reagent that flows out of the area is absorbed by the surrounding highly absorbent area and does not flow to other detection areas and contaminate that area, and the test liquid is also absorbed by the highly absorbent area. , does not remain in the detection area, therefore it is possible to accurately judge the change in color tone of each detection area, and in turn, various detection,
This is effective because measurement work can be done easily and accurately at the same time.

〔Example〕

A biaxially oriented polystyrene sheet was used as the support. Various ink compositions having the following compositions were printed on this sheet by screen printing. Application amount is 2011 on dry matter basis
/It was a. The resulting printed matter was dried at 60° C. for 30 minutes to form striped detection areas and high water absorption poles. The thickness of each strip was 60 μm, and the width of the stripes was 2 mm. Thereafter, this was cut to prepare a test specimen as shown in FIG.

Ink composition for forming highly water-absorbent areas Sumikagel SP Taco O (Sumitomo Chemical) 3 o parts by weight Microcrystalline cellulose PH-OAM (Asahi Kasei) y.

Byron 3oo (Toyobo) 2 Cyclohexanone 30 parts by weight Tsurupetz≠/Yu 0 2/In addition, ink compositions for glucose detection, ink compositions for protein detection, and ink compositions for pH measurement have been used in the above-mentioned patent application Sho&? The material described in Example 3, q of Specification No. 33717 was used. All of these compositions were prepared by pulverizing and dispersing them using a homomixer.

The test specimen thus produced was immersed in urine, pulled out, left horizontally for about 30 minutes, and then observed for coloring.
Each detection area shows normal coloring, with no contamination from other detection areas. Furthermore, there was no mottling or discoloration due to residual droplets in the detection area.

For comparison, a test specimen was prepared in the same manner as described above except that the superabsorbent material was not provided. In this case, when the test was immersed in urine, contamination occurred due to outflow from the adjacent detection area, resulting in discoloration, etc. This caused accurate detection and measurement.

Although the present invention has been mainly described with respect to the detection of various components in body fluids such as urine, the test specimen of the present invention can also be successfully used for detecting various components in other test liquids.

[Brief explanation of drawings]

Figure 1 is a plan view of an embodiment of the test specimen of the present invention;
The figure is a plan view of the embodiment at the same location. /, lI...test body, /2...support, 3.
lI, t. /3. /II, 7! ...detection area, 6. /6...
・Highly absorbent part. Applicant's agent Kiyoshi Inomata 62 Figure

Claims (1)

[Scope of Claims] 1. A test specimen characterized in that a plurality of detection regions each made of a different reagent are provided on a support, and a highly water-absorbing region is provided around each detection region. 2. The test specimen according to claim 1, wherein the support is made of a non-absorbent material, and the detection region and the highly water-absorbing portion are provided at a constant interval. 3. The test specimen according to claim 1, wherein the super absorbent portion contains a super absorbent polymer with a water absorption capacity of 10 times or more.