JPH079425B2 - Test body - Google Patents

Description

TECHNICAL FIELD OF THE INVENTION The present invention relates to a test body capable of simply and accurately detecting various components in a test liquid, and particularly to glucose, protein, occult blood, urobilinogen in body fluid, pH of body fluid and the like. The present invention relates to a test body capable of simultaneously and accurately detecting.

Technical background of the invention and its problems When discovering, diagnosing and treating a disease, it is extremely important to easily and quickly know the presence and amount of various components contained in body fluids such as blood, lymph and urine. That is.

Rapid and easy knowledge of the amount of glucose in body fluids such as urine or blood is essential for early detection, diagnosis and management of diabetes. Also, knowing the amount of protein in body fluids, especially urine, quickly and easily plays a major role in early detection and diagnosis and treatment of renal diseases. Furthermore, knowing the pH of body fluids, especially urine, helps to know whether or not proteins are present in body fluids, and the possibility of bacteriuria that causes nephritis, cystitis, etc. Helps to confirm. Furthermore, knowing the presence or absence of occult blood in body fluids, especially urine, is an early detection of renal disorders,
It plays a major role in diagnosis and treatment, and knowing the amount of urobilinogen in body fluids, especially in urine, plays a major role in the diagnosis of liver function.

Thus, it is extremely important to easily and quickly detect the pH of glucose, protein, occult blood, urobilinogen or body fluid in body fluids, especially urine. Test pieces that have been attached have been used. This test piece has the advantages that it is easy to use and can be judged in a short time.

However, on the other hand, there are some drawbacks, such as the impregnating liquid is unstable and the manufacturing process is complicated, the color changes due to exposure to the atmosphere during storage, the sensitivity is low, and the color development takes time. There were various difficulties. In addition, test pieces were made for each of the various tests, and it took time and labor to perform the various tests.

The inventor of the present invention first developed a test sample which could solve these problems and could carry out various tests easily and quickly at the same time, and filed a patent application (Japanese Patent Application No. 59-33787). This is a method in which a reagent composition for detecting various components is dissolved or dispersed in a solvent to prepare an ink composition, which is printed or coated on a support at a certain interval.

More specifically, this test body is characterized by having a region in which at least one or more of the ink compositions (a) to (c) having the following compositions are applied on a support.

(A) a glucose detection ink composition in which a reagent composition comprising a sugar oxidase, a peroxidase, an oxidizable indicator, a binder and a stabilizer is dissolved or dispersed in a non-aqueous solvent, (b) a protein error An ink composition for protein detection, which comprises a reagent composition comprising an indicator, a pH buffer, a protein-adsorptive ion exchanger, a binder and a water-absorbing powder dissolved or dispersed in a solvent, (c) a pH indicator, Quaternary ammonium salt or amine salt,
An ink composition for pH measurement, wherein a reagent composition comprising a binder and a water-absorbing powder is dissolved or dispersed in a solvent.

In a preferred specific embodiment, this test body is a glucose detection region formed by coating the glucose detection ink composition on a support, a protein detection region formed by coating the protein detection ink composition, and pH measurement. Has a pH measurement area formed by applying the ink composition for use, and in some cases, a urobilinogen detection area formed by applying the urobilinogen detection ink composition or an occult blood detection area formed by applying the occult blood detection ink composition. Have.

In making this test body, for example, a support made of paper synthetic paper, non-woven fabric, synthetic resin film or the like is coated with a glucose detection ink composition by a roll coating method, a spray coating method, a silk screen printing method, or an intaglio printing method. A glucose detection area is formed by applying or printing by a printing method such as a printing method or a gravure printing method, and similarly, a protein detection area and a pH measurement area are formed at regular intervals.

When the test body thus prepared is dipped in urine, for example, if glucose or protein is present in the urine, the reagents in the respective detection regions are discolored and each of them can be rapidly detected.
Similarly, the pH of urine can be easily measured. Therefore, this test sample is very effective because it can simultaneously and quickly measure glucose, protein and pH in urine.

Thus, when forming a plurality of detection regions formed by using different reagents on one support, a plurality of detection or measurement operations can be performed by immersing the support once in urine. Although it is efficient to do at the same time, on the other hand, a small amount of the detection reagent in a certain area flows out to other detection areas together with urine and contaminates the detection reagent, inhibiting the coloring reaction of the contaminated detection reagent, There is a risk of causing an error in color tone.

[Object of Invention and Outline thereof]

Thus, in view of such circumstances, the present invention provides a test body in which a plurality of detection regions made by using different reagents are formed on one support, and each detection region has a reagent outflow of another detection region. It is an object of the present invention to provide a test body which is not contaminated by the reaction, and therefore can proceed without any inhibition of the coloring reaction of each detection reagent and can simultaneously and accurately perform each detection or measurement. To do.

Therefore, the present invention provides a test body characterized in that a plurality of detection regions made of different reagents are provided on a support, and a highly water-absorbing portion is provided around each detection region. is there.

[Specific Description of the Invention]

The present invention will be described in detail with reference to FIG. 1 showing an embodiment thereof. Reference numeral 1 denotes a strip-shaped test body and support 2
Three detection regions 3, 4 and 5 formed by applying or printing different reagents or reagent compositions are provided on each. Here, a strip having a constant width is formed from the upper end to the lower end, and the respective regions are provided in a striped pattern at regular intervals. Typically, the first detection region 3 is a glucose detection region, the second detection region 4 is a protein detection region, and the third detection region 5 is a pH measurement region.

In the present invention, the highly water-absorbent portion 6 is provided around each of the detection areas 3, 4, and 5.
... is provided. That is, the highly water-absorbing portions 6 are provided between adjacent detection areas, on the left side of the first detection area 3 and on the right side of the third detection area in strips or stripes having a constant width. In that case, a certain space is provided between each detection region and each high water absorption portion so that they do not come into direct contact with each other.

As shown in FIG. 2, a plurality of rectangular or substantially square detection regions 13, 14, 15 are provided in the widthwise center of the non-water-absorbent support 12 of the strip-shaped test body 11, and the four sides thereof are surrounded. It is also possible to enclose the above with the highly water absorbent portion 16. At this time, each detection region is also provided with a constant interval between the high water absorption part surrounding a certain detection region and the high water absorption part surrounding the adjacent detection region.

The support used here is preferably made of a non-water-absorbing material such as various synthetic resin sheets, and the high water-absorbing part is made of a high water-absorbing polymer having an ability to absorb pure water 10 times or more its own weight. It is preferably made of such a superabsorbent material. If a certain distance is provided between the detection regions 3, 4, 5 and the high water absorption portion 6 as described above, a non-water-absorbing support will be interposed therebetween, so that this test body is immersed in urine. However, urine is distributed between the detection area and the superabsorbent section, and thus the outflow of the reagent from the detection area can be prevented.

If there is no non-water-absorbent material between the detection area and the superabsorbent member and they are in direct contact with each other, the detection reagent between the detection area and the superabsorbent member after immersion in the test solution such as urine Movement occurs,
Thus, contamination of the adjacent detection area occurs. On the other hand, if there is no highly absorbent portion and only the non-water-absorbing material is present on both sides of the detection area, the test liquid (body fluid) remaining in the detection area is less absorbed. This affects the color development performance, especially when glucose is detected.

As described above, the presence of the non-water-absorbing material serves to properly distribute the test liquid on the test body between the detection region and the high-absorption member and prevent the reagent from flowing out from the detection region.

The ink composition for forming the highly water-absorbing part 6 includes a water-absorbing powder such as cellulose, starch and super-water-absorbing gel, a hydrophilic or hydrophobic resin, a binder and an inorganic filler for making the film porous. Such additives and solvents are included.

The super absorbent gel used here is polyvinyl alcohol (PV
A), a cross-linked or graft copolymer mainly composed of acrylic acid salt, acrylonitrile, starch and the like, and as a commercially available super absorbent gel, Sumika Gel (registered trademark) SP520 (having a water absorption capacity of 600 times) PVA-acrylate block copolymer commercially available from Sumitomo Chemical Co., Ltd., Sunwet (registered trademark) IM-1000 having a water absorption capacity of 1000 times
(Acrylic acid grafted starch commercially available from Sanyo Kasei Co., Ltd.), KI gel (trademark) (PV that also has a water absorption capacity of 1000 times)
A reaction product with A-cyclic acid anhydride) and the like.

〔The invention's effect〕

According to the present invention, a plurality of detection regions made of different detection reagents are provided on a support, and a highly water-absorbing portion is provided around the detection regions, so that the detection regions are immersed in a test liquid such as urine. A small amount of reagent that flows out from is absorbed by the surrounding high-absorbent portion, does not flow out to other detection areas and contaminates that area, and the test liquid is also absorbed by the high-absorbent portion, It does not remain in the detection area, so it is possible to accurately judge the change in color tone of each detection area, and by extension various detections.
This is effective because the measurement work can be performed easily and accurately at the same time.

〔Example〕

Biaxially oriented polystyrene sheet was used as a support. Various ink compositions having the following compositions were printed on this sheet by a screen printing method. The coating amount was 20 g / m ² on a dry matter basis. The resulting printed matter was dried for 30 minutes at 60 ° C. to form striped patterns of each detection region and superabsorbent part. Each thickness is 160μm,
The width of the stripe was 2 mm. Thereafter, this was cut to prepare a test body as shown in FIG.

Ink composition for forming highly water-absorbing part Sumikagel registered trademark SP520 (Sumitomo Chemical) 30 parts by weight Microcrystalline cellulose PH-06M (Asahi Kasei) 10 Byron (registered trademark) 300 (Toyobo) 9 Cyclohexanone 30 parts by weight Solvetso (trademark) # 150 21 In addition, the glucose detection ink composition, the protein detection ink composition, and the pH measurement ink composition described in Examples 1, 3 and 4 of Japanese Patent Application No. 59-33787 are used. I was there. Each of these compositions was prepared by homogenizing and dispersing with a homomixer.

The test body produced in this manner was immersed in urine and pulled up, and after standing horizontally for about 30 minutes, the coloring situation was observed.Each detection area shows a normal coloring situation, and contamination from other detection areas does not occur. I was not able to admit. In addition, there was no mottle or fading due to the residual droplets in the detection area.

For comparison, a test piece was prepared in the same manner as above except that the super absorbent member was not provided. In this case, when it was immersed in urine and tested, contamination due to outflow from the adjacent detection region occurred, resulting in discoloration and the like, and accurate detection and measurement could not be performed.

Although the present invention has been mainly described with respect to the detection of various components in body fluids such as urine, the test body of the present invention can be favorably used for the detection of various components in other test liquids.

[Brief description of drawings]

FIG. 1 is a plan view of an embodiment of the test body of the present invention, FIG.
The drawing is a plan view of another embodiment. 1,11 …… Test body, 2,12 …… Support body, 3,4,5,13,14,15 ……
Detection area, 6,16 ... High-absorbency part.

Claims (2)

[Claims] 1. A plurality of detection regions made of different reagents are provided on a support, and a highly water-absorbing portion containing a highly water-absorbent polymer having a water absorption capacity of 10 times or more is provided on the outer periphery of each detection region. A test body characterized by. 2. The test body according to claim 1, wherein the support is a non-absorbent substance, and the detection region and the highly water-absorbing portion are provided at a constant interval.