JPS61212275A - Bioreactor furnished with actuator - Google Patents

Abstract

PURPOSE:To provide the titled small-sized reactor having simple structure and high airtightness to prevent the intrusion of sundry germs, by forming a rotary motor composed of a stator placed outside of the reactor vessel and a rotor placed in the vessel interposing a nonmagnetic and nonconductive vessel wall between the stator and the rotor. CONSTITUTION:A stator 14 is fixed to the outer circumference of the rotor-space 12 of a closeable vessel 10 made of a nonmagnetic and nonconductive material. A rotor 16 is placed rotatably in the rotor space 12, and the core and coil of the rotor 16 is coated with a synthetic resin 17. The stator 14 is electrified to form a rotating magnetic field and the rotor 16 and the stirring blade 18 are rotated to agitate the reaction solution in the vessel 10.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a bioreactor equipped with an actuator that makes it possible to reliably prevent invasion of germs and outside air.

(Background of the Invention) Conventionally, there are bioreactors that are equipped with an actuator for obtaining rotational force within a container, and that use this actuator to react while stirring a reaction solution to promote an enzyme reaction or the like. In this type of bioreactor, it is necessary to prevent bacteria from entering the reaction solution, and in particular, in the case of an anaerobic reaction, it is required to ensure isolation from the atmosphere. Furthermore, in the case of a pressurized reaction, leakage of the reaction solution must be reliably prevented. For this reason, it is not possible to drive the stirrer inside the container from outside the container via a rotating shaft that passes through the bottom of the solution or the lid.
The seal on the rotating shaft comes into contact with the reaction solution, which not only increases the risk of bacteria invading, but also reduces airtight reliability due to abrasion of the seal, which is undesirable.

Therefore, conventionally, as shown in FIG. 7, a permanent magnet 4 facing a permanent magnet 3 rotated by a rotary electric motor 2 is disposed outside the container 1, and stirring is performed by rotating the permanent magnet 4. A permanent magnet coupling has been proposed. However, in this case, the electric motor 2
The problem is that the entire device becomes larger. (Objective of the Invention) The present invention has been made in view of the above circumstances, and provides a bioreactor with an actuator that is free from the risk of bacterial invasion, has a highly reliable airtight container, is small in size, and has a simple configuration. The purpose is to

(Structure of the Invention) According to the present invention, this object is achieved by providing a bioreactor with an actuator that includes an actuator that generates a rotational output inside the container, while forming at least a part of the container from a non-magnetic and non-conductive material; , with an actuator characterized in that a stator and a rotor are respectively disposed on the outside and inside of the container with the non-magnetic and non-conductive material portion sandwiched therebetween, and the stator and rotor form a rotating electric motor. Achieved by bioreactor.

(Example) FIG. 1 is a side sectional view of one embodiment of the present invention.

In this figure, numeral 10 is glass, some kind of stainless steel plate,
A sealable container made of non-magnetic and non-conductive material such as synthetic resin. A cylindrical rotor accommodating portion 12 is formed at the bottom of the container 10 and projects downward. A stator 14 is fixed to the outer periphery of the cylindrical portion of the rotor accommodating portion 12, and a rotor 14 is fixed inside the rotor accommodating portion 12.
6 is rotatably housed. These stator 14 and rotor 16 sandwich the container 10 to form a known cylindrical gap cage cage induction motor. That is, the rotor 16 is
A coil is provided to flow an induced current due to the rotating magnetic field formed by the stator 14. This rotor 16 is entirely hardened with synthetic resin 17 so as to surround its core and coils. The synthetic resin 17 is preferably a fluororesin, which has low frictional resistance with the inner surface of the container 1o and has excellent wear resistance and corrosion resistance.

A stirring blade 18 is fixed to a shaft 20 extending upward from the center of the rotor 16.

Therefore, when the stator 14 is supplied with current from a control circuit (not shown) and the stator 14 forms a rotating magnetic field, the container 10
The rotor 16 and stirring blades 18 inside rotate. Therefore, the reaction solution in the container 10 is stirred, and the reaction is promoted.

In this embodiment, the center of the magnetic field of the stator 14 is located at the rotor 1.
They are mutually positioned so that they are higher than the center of the magnetic field of No. 6 by h. Therefore, the rotor 16 receives an upward force due to the difference in the centers of the magnetic fields, and the frictional resistance between the rotor 16 and the bottom surface of the rotor accommodating portion 12 decreases, causing the rotor 16 to move upward. The effect of smooth rotation can be obtained.

FIG. 2 is a side sectional view of a second embodiment of the invention. In this embodiment, a stator L4A is disposed below the flat bottom surface of the container 10A, and a rotor 16A is disposed above the stator L4A so as to sandwich the flat bottom surface of the container 10A, thereby creating a radial gap that extends in the half-body direction with respect to the rotation center line. A permanent magnet synchronous motor of the type is formed. That is, the rotor 16A is made of a permanent magnet whose polarity changes alternately in the circumferential direction, and the stirring blade 18A is integrally fixed to the upper surface of the rotor 16A. The rotor 16A is supported by a support shaft 2OA protruding from the bottom of the container 10A via a completely waterproof bearing.

In the conventional device shown in FIG. 7, it is very difficult to detect even if the permanent magnets 3.4 are out of synchronization and a state of adjustment occurs, but according to this embodiment, when the stator 14A is The tax adjustment can be easily detected from current changes, and rotation in a synchronous state is always possible by correcting the rotational speed of the rotating magnetic field of the stator 14A.

FIG. 3 is a side sectional view of the third embodiment, and the actual flow side has an inverted truncated conical rotor accommodating portion 12B on the bottom surface of the container 10B.
The rotor 1.6B, which is made of a permanent magnet, is similarly formed in the shape of an inverted truncated cone. According to this embodiment, the radial movement of the rotor 16B is caused by the rotor accommodating portion 12B.
is regulated by the conical surface of the rotor 16B, and a self-centering effect of the rotor 16B is obtained. Note that 14B is a stator, and 18B is a rotor 16.
A stirring blade is fixed to the top surface of B.

Fig. 4 is a side sectional view of the fourth embodiment, and Fig. 5 is its rotor 1.
6C is a bottom view. In this embodiment, a through hole 22 is formed on the central axis of the rotor 16C, and a plurality of radial notch grooves 24 are formed on the lower surface of the rotor 16C. According to this embodiment, the reaction solution flows in the radial direction from the through hole 22 through the cutout groove 24 and is stirred. Therefore, solids contained in the reaction solution do not precipitate between the rotor 16C and the bottom surface of the container 10, and stirring becomes more reliable.

FIG. 6 is a side sectional view of the fifth embodiment. In this embodiment, a stepped rotor accommodating part 12D is formed in the cylindrical part of the container 10D, a rotor 16D made of an annular permanent magnet is placed therein, and stirring blades 18D are mounted on the inner peripheral surface of the rotor 16D. is fixed in the inner diameter direction. Note that 14D is a stator.

In each of the above embodiments, the container 10. Although the entire 10A-D is made of a non-magnetic and non-conductive material, at least only the portion sandwiched between the stator and the rotor is made of this material,
Of course, other parts may be formed of other materials.

Further, the rotational output of the rotor may be used not only as a stirring blade but also as another power source, and the present invention also includes such a power source. For example, this rotational output may be used as a driving source for a pump housed in a container.

(Effects of the Invention) As described above, the present invention has a stator on the outside and a rotor on the inside, sandwiching the non-magnetic and non-conductive material part of the container, and these form a rotating electric motor. Therefore, there is no need for a rotating shaft passing through the container, there is no fear of invasion of germs, and the airtightness of the container can be improved. Further, unlike the conventional device, there is no need to provide an independent electric motor externally, so the entire device can be made smaller and the configuration can be simplified.

For example, there is no need for a rotating shaft that passes through the container, its bearings, or seals, and the inertial weight of the rotating parts is reduced, resulting in improved efficiency (responsiveness is improved, and the number of permanent magnets is smaller than in conventional permanent magnet couplings). Reduce by half.

[Brief explanation of the drawing]

1 to 4.6 are side sectional views of embodiments of the present invention, respectively;
FIG. 5 is a bottom view of the rotor of the embodiment shown in FIG. 4, and FIG. 7 is a partially sectional side view of the conventional device. 10, l0A-D... Container, 14.14A-D... Stator, 16.16A-D... Rotor.

Claims (4)

[Claims] (1) In a bioreactor with an actuator that includes an actuator that generates rotational output inside the container, at least a part of the container is formed of a non-magnetic and non-conductive material, and a portion of the non-magnetic and non-conductive material A bioreactor with an actuator, characterized in that a stator and a rotor are disposed on the outside and inside of the container, respectively, with the stator and rotor forming a rotating electric motor. (2) The bioreactor with an actuator according to claim 1, wherein the rotary motor is an induction motor and the rotor is a cage rotor. (3) The bioreactor with an actuator according to claim 1, wherein the stator forms a rotating magnetic field, the rotor is made of a permanent magnet, and the stator and rotor form a permanent magnet type synchronous motor. . (4) A bioreactor with an actuator according to claims 1 to 3, wherein the surface of the rotor is covered with a synthetic resin.