JPS61212248A - Nutrient composition for animal - Google Patents

Abstract

PURPOSE:To provide the titled inexpensive composition, containing both threonine and lysine, exhibiting the nutrient effect comparable to that of the 1:1 mixture of the amino acids and meeting the taste of animals, by culturing a microbial strain capable of producing threonine and lysine at the same time and drying the obtained culture liquid. CONSTITUTION:A microbial strain capable of producing threonine and lysine at the same time (e.g. strain of FERM-P 4190) is cultured, and the produced culture liquid is adjusted preferably to 4-6pH, concentrated, dehydrated and dried usually under reduced pressure, to obtain the objective composition composed of impurities originated from the culture liquid and the dried aqueous solution of amino acid mixture containing at least threonine and lysine. It is preferable to use a saccharified starch solution as a carbon source to prevent the coloring and side reactions.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to a novel animal nutritional composition that can be used as an additive to animal feed.

Prior Art Threonine and lysine are essential amino acids for animals such as pigs. Adding threonine and lysine to animal feed,
It is known that when used together, it is more effective for nutritional effects such as weight gain in animals when they are used in combination than when used individually (M, F, Fuller
et at, + Br, J, Nutr, + 41 +3
33.1979: G. J. Bonneau at a
t,, The 62ndAnimal F@eder
s' day Report, P, 132+1983
etc.). Under these circumstances, both are manufactured separately and used in combination.

Problems to be Solved by the Invention An amino acid mixture that contains both threonine and lysine, has nutritional effects comparable to a mixture of equal amounts of threonine and lysine, has stable physical properties, and is preferred by animals. There is a need for the development of a technology to easily manufacture the product at low cost.

The present inventor has discovered that a dried aqueous amino acid solution obtained from a culture solution of a microorganism that has the ability to simultaneously produce threonine and lysine, and containing impurities derived from the culture solution and at least threonine and lysine as amino acids, is an excellent nutritional product. The present inventors have discovered that the product is effective, that impurities derived from the culture solution do not cause problems in feed, and that it has physical properties that do not cause problems during long-term storage.Based on this discovery, the present invention was completed. reached.

Microorganisms that can be used in the present invention need only have the ability to simultaneously produce at least threonine and lysine.5For example, FERM-P 4190, FERM-P 418
2.

FERM-P 4183, FERM-P 4184
, FERM-P 4185.

The culture method is not particularly difficult; for example, Japanese Patent Application Laid-Open No. 54-352
The methods described in No. 85, No. 54-35286, and No. 54-35288 may be used. In this case, any carbon source that can be converted by the microorganism can be used. However, 1. For example, crystalline glucose, sucrose, and starch saccharified liquid have little residue after the fermentation process,
Preferable for preventing coloring and side reactions. The culture solution thus obtained may be used as it is or may be subjected to a drying process after primary seedling formation. At this time, the ratio of threonine and lysine is 1:1 to 10
It is preferably contained in a weight ratio of 8 to 10 to 40 to 1 O. Further, the total amino acid content in the amino acid mixed aqueous solution is about 20 to 70% of the total solid content, preferably 30 to 60%.
Contains some degree.

Further, when concentrating the culture solution, it is desirable to adjust the pH of the culture solution to a value of 4 to -6 in advance from the viewpoint of preventing coloring of the product. - The acid used for adjustment must not contain substances harmful to animals, such as hydrochloric acid or caustic soda, and must not have the possibility of reacting with amino acids.

The concentration temperature is about 110 to 20°C, which is about 80 to 40°C in order to prevent decomposition and improve concentration efficiency.

Concentration and dehydration can of course be carried out under reduced pressure, but adding substances to the culture solution that are not harmful to animals and that form an azeotrope with water and lower the boiling point can lower the solution temperature and
This is effective in preventing loss of coloring and decomposition of products.

The product obtained in this way is stable, is liked by pigs, has the same nutritional value as a mixture with the same ratio of threonine and lysine, and has no other impurities that may interfere with the feed. There isn't.

On the other hand, 1 For example, in the case of barley-based feed for fattening pigs 1
Lysine is usually the first limiting amino acid.

The most effective amount of threonine added is about the same amount as a non-fishing machine, but in this case, the threonine of the present invention is added.

Lysine-containing composition (threonine:lysine=100:30
), the amount of lysine added can be reduced by about 16 units, which is extremely effective in reducing the cost of feed.

Example Hereinafter, the present invention will be explained in detail with reference to Examples.

Example 1 Threonine production medium 81 having the composition shown in Table 1 below was mixed with 2
0 l Jarfsrmentsr and after sterilization 1
Aeration vvva stirring 350 rpm, temperature 31°5°C
While controlling with NH3 water at pH 7.0,
Microorganism FERM-P 419 described in Publication No. 35288
0 was cultured.

Glucose solution was continuously fed during the culture.

A culture solution 101 containing 31//di of threonine and 1.017 di of lysine was obtained.

Table 1 Thr-Lys fermentation liquid medium composition Glucose
l0II/d/! (NHa)2804
3 〃KH2PO40,1// Mg504-7H200,1'/ Fe1o4・7 H2O0-001ttMnSO4'4
H200,001tt Psymine hydrochloride 300μg/l Bithion 100μm1/1 Antifoaming soybean oil
Using 0.01 d/dt threonine 6 lysine fermentation liquid 101, I! by adding hydrochloric acid! After setting it to 15, it becomes 60% moisture.
After concentrating under reduced pressure to
It was dry to the touch. In this process, when the moisture content is 5 g or more, the product temperature is below 60°C, and when the moisture content is below 5 g, the product temperature is 8 ℃.
The degree of vacuum and heating temperature were adjusted so that the temperature was below 0°C.

By the above method, threonine and lysine containing composition 10
01 (threonine 2401, lysine 809) was obtained.

The threonine and lysine content per total solid fraction of the threonine and lysine powder obtained in this way are the same as the threonine and lysine content of the total solid fraction in the fermentation liquid after one adjustment, and the degree of coloring by concentration drying Almost no increase was observed.

On the other hand, the above example was repeated without making any changes except that the voice value of the concentration step was adjusted to 7. A product with slightly noticeable coloring was obtained.

Furthermore, the threonine and lysine powder of the present invention did not have any physical properties that would cause any inconvenience when added to feed.

Example 2 Feeding test A mixture of the product of the present invention and L-lysine hydrochloride was added as threonine to a basal feed containing 97% barley, a mineral mixture, and a vitamin mixture with a crude tanning content of 9.5%.
．． 18% and lysine 0.4%, and the nutritional effect on fattening pigs was tested.

The test results are as follows.

(Test method) Using 4 fattening pigs (2 castrated males, 2 females) in 1 section, 25
kg to 60kl? The animals were reared until the age of 10, and changes in their average body weight were measured.

(Results) The mixture of the product of the present invention and L-L7 back Ct was found to have the same effect of improving daily wage and feed conversion ratio as the mixture of L-LysHCl and L-Thr at the same mixing ratio. It was done.

Effects of the Invention According to the present invention, threonine and lysine are not separately isolated and purified, that is, there is no need for a purification step, and there is no accompanying loss of amino acids. It is prepared at low cost and by simple operations, and the nutrients of threonine and lysine can be utilized to the maximum extent possible. Therefore, it is clear that the present invention is extremely useful industrially.

Claims (1)

[Claims] 1. Obtained from a culture solution of a microorganism that has the ability to simultaneously produce threonine and lysine, and containing impurities derived from the culture solution and a dried product of an amino acid mixed aqueous solution containing at least threonine and lysine as amino acids. Animal nutritional composition. 2. The composition according to claim 1, which contains threonine and lysine in a weight ratio of 1:1 to 10:1. 3. The composition according to claim 1, wherein the amino acid content in the amino acid mixed aqueous solution is 20 to 70% based on the total solid content. 4. The composition according to claim 1, wherein the pH value of the step of concentrating the aqueous solution in the preparation of the dried product is within the range of 4 to 6.