JPS61159135A - Particle analyzing device - Google Patents

Particle analyzing device

Info

Publication number
JPS61159135A
JPS61159135A JP59281457A JP28145784A JPS61159135A JP S61159135 A JPS61159135 A JP S61159135A JP 59281457 A JP59281457 A JP 59281457A JP 28145784 A JP28145784 A JP 28145784A JP S61159135 A JPS61159135 A JP S61159135A
Authority
JP
Japan
Prior art keywords
sample liquid
flow
laminar flow
liquid
television
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP59281457A
Other languages
Japanese (ja)
Inventor
Yuji Ito
勇二 伊藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canon Inc
Original Assignee
Canon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Canon Inc filed Critical Canon Inc
Priority to JP59281457A priority Critical patent/JPS61159135A/en
Publication of JPS61159135A publication Critical patent/JPS61159135A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

PURPOSE:To easily decide on the stable state of a laminar flow by picking up the flow of sample liquid by a television camera which is arranged at right angles to the center axis of the flow of sample liquid and displaying its image on a television monitor. CONSTITUTION:A sample liquid intake 2 and a sheath liquid intake 3 are connected to a nozzle 1 and the sample liquid S flows out of the orifice atop the nozzle 1 while covered with sheath liquid as a fast laminar flow. An image forming lens 5 is arranged on the optical axis O of laser light L projected by a laser light source 4 arranged in the direction intersecting orthogonally with the center axis Z of the flow and a condenser lens 6 and a photoelectric detector 7 are arrayed opposite the light source 4. Further, a photographic lens 8 and a television image pickup tube 9 are arranged approximately in the direction intersecting orthogonally with the center axis Z and optical axis O. The state of the flow of the sample liquid S covered with the sheath liquid is projected on the television monitor through the image pickup tube 9 and it is judged whether or not the sample liquid S flows properly on a measured waveform display tube which displays the output of the detector 7.

Description

【発明の詳細な説明】 ［産業上の利用分野］ 本発明は、フローサイトメータ等において、サンプル液
が安定した層流をなして流れているか否かを容易に判定
することを可能とした粒子解析装置に関するものである
。[Detailed Description of the Invention] [Industrial Application Field] The present invention provides particles that make it possible to easily determine whether a sample liquid is flowing in a stable laminar flow in a flow cytometer or the like. This relates to an analysis device.

［従来の技術］ フローサイトメータとは、高速で流れる細胞浮遊溶液、
即ちサンプル液に例えばレーザー光を照射し、その散乱
光・蛍光による光電信号を検出し、細胞の性質・構造を
解明する装置であり、細胞化学、免疫学、血液学、腫瘍
学、遺伝学等の分野で使用されている。[Conventional technology] A flow cytometer is a cell suspension solution that flows at high speed.
In other words, it is a device that irradiates a sample liquid with, for example, a laser beam, detects a photoelectric signal from the scattered light and fluorescence, and elucidates the properties and structure of cells, and is used in cytochemistry, immunology, hematology, oncology, genetics, etc. used in the field of

フローサイトメータにおいては、照射光の光軸に対する
サンプル液の流れる軸の位置を一定に保つことは正確な
測定上欠くことのできない要件であり、そのためにシー
ス液を加圧し層流の性質を保持させながら高速でノズル
先端を流し、この流れの中央にサンプル液を注ぐ方式が
採用されている。このようにすると、サンプル液は高速
で層流の中心軸に沿って流れることになり、このときサ
ンプル液の流速を絞り込み安定した層流を得るために、
サンプル液とシース液との流量比を１対５０程度に保ち
、直径５０４ｍ程度の小さなノズル内を秒速１５ｍ程度
で通過させる。In a flow cytometer, it is essential to maintain the position of the sample liquid flow axis constant with respect to the optical axis of the irradiation light for accurate measurement, and for this purpose, the sheath liquid is pressurized to maintain laminar flow properties. The system uses a method in which the nozzle tip is flowed at high speed while the sample liquid is poured into the center of this flow. In this way, the sample liquid will flow at high speed along the central axis of the laminar flow. At this time, in order to reduce the flow rate of the sample liquid and obtain a stable laminar flow,
The flow rate ratio of sample liquid to sheath liquid is maintained at about 1:50, and the sample liquid and sheath liquid are passed through a small nozzle with a diameter of about 504 m at a speed of about 15 m/s.

このように安定した層流を得るための初期条件を設定し
ても、装置を含む周囲雰囲気の微小変動により、計測期
間中に層流の微小変動が起り得る。そのために常に層流
の状態を監視する必要があり、特にジェット・イン０工
ア方式のフローサイトメータは、空気中を流れるサンプ
ル液に照射光を照射する方式であるので、周囲空気によ
り層流に微小変動が起り易く、散乱光・蛍光からの信号
解析の精度が低下する傾向が大きいためその監視体制が
不可欠である。Even if the initial conditions for obtaining a stable laminar flow are set as described above, small fluctuations in the laminar flow may occur during the measurement period due to small fluctuations in the surrounding atmosphere including the device. For this purpose, it is necessary to constantly monitor the state of laminar flow. In particular, jet-in-air type flow cytometers irradiate the sample liquid flowing in the air with irradiation light, so the laminar flow is caused by the surrounding air. A monitoring system is essential because minute fluctuations are likely to occur, and the accuracy of signal analysis from scattered light and fluorescence tends to decrease.

従来、このような層流の安定性の監視には、顕微鏡によ
る肉眼観察という方法が採用されていた。そのため安定
性の判定にばらつきが生じ易く、しかも判定に時間が掛
かり、迅速な層流補正が不可能で十分に精度の良い計測
ができなかった。Conventionally, the method of monitoring the stability of such laminar flow has been macroscopic observation using a microscope. As a result, variations in the stability judgment tend to occur, and it takes time to make the judgment, making it impossible to quickly correct the laminar flow and making it impossible to measure with sufficient accuracy.

［発明の目的］ 本発明の目的は、サンプル液の流れの中心軸に直交して
配置したテレビカメラでサンプル液の流れを撮影し、テ
レビモニタ上に表示することにより、層流の安定状態を
容易に判別することを可能とした粒子解析装置を提供す
ることにある。[Objective of the Invention] An object of the present invention is to photograph the flow of a sample liquid with a television camera placed perpendicular to the central axis of the flow of the sample liquid and display it on a television monitor, thereby observing the stable state of laminar flow. An object of the present invention is to provide a particle analysis device that enables easy discrimination.

［発明の概要］ 上述の目的を達成するための本発明の要旨は、サンプル
液をシース液で包んで高速の層流にする手段と、該層流
の流れの状態を機影する手段と、該撮影手段による画像
を表示する手段とを有することを特徴とする粒子解析装
置である。[Summary of the Invention] The gist of the present invention for achieving the above-mentioned object is a means for wrapping a sample liquid in a sheath liquid to create a high-speed laminar flow, a means for monitoring the flow state of the laminar flow, This is a particle analysis device characterized by having a means for displaying an image obtained by the photographing means.

［発明の実施例］ 本発明を図示の実施例に基づいて詳細に説明する。[Embodiments of the invention] The present invention will be explained in detail based on illustrated embodiments.

第１図はテレビ観察系を有する粒子解析装置の主要部の
構成図であり、ノズル１にサンプル液流入口２、シース
液流入口３が接続され、ノズル１の先端のオリフィスか
°ら高速層流となったシース液に包まれてサンプル液Ｓ
が波山するようになっている。この流れの中心軸Ｚと直
交する方向にこのレーザー光源４が配され、レーザー光
Ｒ４から出射されたレーザー光りの光軸０上に、サンプ
ル液Ｓに対して、レーザー光源４側に結像レンズ５が配
置され、レーザー光源４と反対側に集光レンズ６、光電
検出器７が順次に配列されている。更に、サンプル液Ｓ
の流れの中心軸Ｚ、及びレーザー光りの光軸０のそれぞ
れにほぼ直交する方向に、撮影レンズ８、テレビ撮像管
９が配置されている。Fig. 1 is a configuration diagram of the main parts of a particle analysis device having a television observation system.A sample liquid inlet 2 and a sheath liquid inlet 3 are connected to a nozzle 1, and a high-speed layer is formed from an orifice at the tip of the nozzle 1. The sample liquid S is surrounded by the flowing sheath liquid.
is starting to rise and fall. This laser light source 4 is arranged in a direction perpendicular to the central axis Z of this flow, and an imaging lens is placed on the laser light source 4 side with respect to the sample liquid S on the optical axis 0 of the laser light emitted from the laser light R4. A condenser lens 6 and a photoelectric detector 7 are sequentially arranged on the side opposite to the laser light source 4. Furthermore, sample liquid S
A photographing lens 8 and a television image pickup tube 9 are arranged in directions substantially perpendicular to the central axis Z of the flow and the optical axis 0 of the laser beam.

第２図は装置本体の外観斜視図であり、粒子解析装置の
測光部が格納されている本体１０の前面には、ブラウン
管から成るテレビモニタ１１及び測定波形表示管１２が
設置され、その近傍に測光部格納部１３が設けられてい
る。テレビモニタ１１にはテレビ撮像管９の出力が接続
され、測定波形表示管１２には光電検出器７の出力が接
続されている。FIG. 2 is an external perspective view of the main body of the device. A television monitor 11 consisting of a cathode ray tube and a measurement waveform display tube 12 are installed on the front of the main body 10 in which the photometric section of the particle analysis device is housed, and a measurement waveform display tube 12 is installed in the vicinity. A photometry section storage section 13 is provided. The output of the television image pickup tube 9 is connected to the television monitor 11, and the output of the photoelectric detector 7 is connected to the measurement waveform display tube 12.

従って、レーザー光源４から出射されたレーザー光りは
、結像レンズ５によりサンプル液Ｓに照射され、このサ
ンプル液Ｓによって散乱された散乱光は、集光レンズ６
を介して光電検出器７に集光され、サンプル液Ｓの性状
が検出される。なお、この粒子解析には、レーザー光が
有する緋定の測定波長領域の光が使用されている。Therefore, the laser light emitted from the laser light source 4 is irradiated onto the sample liquid S by the imaging lens 5, and the scattered light scattered by the sample liquid S is transmitted through the condensing lens 5.
The light is focused on the photoelectric detector 7 through the light, and the properties of the sample liquid S are detected. Note that, in this particle analysis, light in the measurement wavelength range of a laser beam is used.

一方、シース液に包まれたサンプル液Ｓの流れの状態は
、図示しない照射光源により測定波長領域外の光で照射
され、テレビ撮像管９を介してテレビモニタ１１に拡大
して写し出されると共に、光電検出器７の出力を表示す
る測定波形表示管１２上で、サンプル液Ｓが適正な状態
で流れているかどうかが、その出力波形状態から判定さ
れる。On the other hand, the flow state of the sample liquid S wrapped in the sheath liquid is irradiated with light outside the measurement wavelength range by an irradiation light source (not shown), and enlarged and displayed on the television monitor 11 via the television image pickup tube 9. Whether the sample liquid S is flowing in an appropriate state on the measurement waveform display tube 12 that displays the output of the photoelectric detector 7 is determined from the state of its output waveform.

このようにして、本体１０の前面のテレビモニタ１１及
び測定波形表示管１２を監視することにより、サンプル
液Ｓがシース液による層流の中心部を正確に安定して流
れているか否かを容易に知ることができる。また、層流
の状態を肉眼観察でなく、測定波形によって光電的・数
値的に把握可能であるので、観察結果をフィードバック
して自動的に層流の状態を制御することも可能である。In this way, by monitoring the television monitor 11 and measurement waveform display tube 12 on the front of the main body 10, it is easy to check whether the sample liquid S is flowing accurately and stably in the center of the laminar flow caused by the sheath liquid. can be known. Furthermore, since the state of laminar flow can be grasped photoelectrically and numerically from measured waveforms rather than by visual observation, it is also possible to feed back the observation results and automatically control the state of laminar flow.

なお、実施例においてはジェット・イン・エアタイプの
フローサイトメータについて適用しているが、クローズ
ドタイプのフローサイトメータについても同様に用いる
ことができる。In the embodiment, the present invention is applied to a jet-in-air type flow cytometer, but it can be similarly applied to a closed type flow cytometer.

［発明の効果］ 以上説明したように本発明に係る粒子解析装置は、サン
プル液の流れの中心軸にほぼ直交する位置に配置された
撮影手段により、サンプル液の流れの状態を常時撮影し
表示手段に表示することにより、容易にしかも正確に層
流の安定状態の是非を判定することを可能とし、機器の
操作性を向上させ精度の良い計測を可能としている。[Effects of the Invention] As explained above, the particle analysis device according to the present invention constantly photographs and displays the state of the flow of the sample liquid using the photographing means arranged at a position substantially perpendicular to the central axis of the flow of the sample liquid. By displaying the information on the means, it is possible to easily and accurately determine whether the laminar flow is in a stable state, improving the operability of the equipment, and enabling highly accurate measurement.

【図面の簡単な説明】[Brief explanation of drawings]

図面は本発明に係る粒子解析装置の一実施例を示すもの
であり、第１図は主要部の構成図、第２図は本体の外観
斜視図である。 符号ｌはノズル、２はサンプル液流入口、３はシース液
流入口、４はレーザー光源、５は結像レンズ、６は集光
レンズ、７は光電検出器、８は撮影レンズ、９はテレビ
撮像管、ｌＯは本体、１１はテレビモニタ、１２は測定
波形表示管である。The drawings show an embodiment of the particle analysis apparatus according to the present invention, and FIG. 1 is a configuration diagram of the main part, and FIG. 2 is an external perspective view of the main body. Symbol l is a nozzle, 2 is a sample liquid inlet, 3 is a sheath liquid inlet, 4 is a laser light source, 5 is an imaging lens, 6 is a condensing lens, 7 is a photodetector, 8 is a photographing lens, 9 is a television Reference numeral 10 indicates an image pickup tube, 10 indicates a main body, 11 indicates a television monitor, and 12 indicates a measurement waveform display tube.

Claims (1)

【特許請求の範囲】 １、サンプル液をシース液で包んで高速の層流にする手
段と、該層流の流れの状態を撮影する手段と、該撮影手
段による画像を表示する手段とを有することを特徴とす
る粒子解析装置。 ２、前記撮影手段は測定用レーザー光の光軸及び前記サ
ンプル液の層流方向とそれぞれ直交する方向に配置した
特許請求の範囲第１項に記載の粒子解析装置。 ３、前記撮影手段を撮影レンズ及びテレビ撮像管により
構成した特許請求の範囲第１項に記載の粒子解析装置。 ４、前記撮影手段は測定に使用する波長領域外の光で照
射される前記層流の流れの状態を撮影するようにした特
許請求の範囲第１項に記載の粒子解析装置。 ５、前記撮像表示手段をテレビモニタとした特許請求の
範囲第１項に記載の粒子解析装置。[Scope of Claims] 1. It has a means for wrapping a sample liquid in a sheath liquid to create a high-speed laminar flow, a means for photographing the state of the laminar flow, and a means for displaying an image obtained by the photographing means. A particle analysis device characterized by: 2. The particle analysis apparatus according to claim 1, wherein the photographing means is arranged in a direction perpendicular to the optical axis of the measurement laser beam and the laminar flow direction of the sample liquid. 3. The particle analysis apparatus according to claim 1, wherein the photographing means is constituted by a photographing lens and a television image pickup tube. 4. The particle analysis apparatus according to claim 1, wherein the photographing means photographs the state of the laminar flow irradiated with light outside the wavelength range used for measurement. 5. The particle analysis apparatus according to claim 1, wherein the image capturing and displaying means is a television monitor.
JP59281457A 1984-12-31 1984-12-31 Particle analyzing device Pending JPS61159135A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP59281457A JPS61159135A (en) 1984-12-31 1984-12-31 Particle analyzing device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59281457A JPS61159135A (en) 1984-12-31 1984-12-31 Particle analyzing device

Publications (1)

Publication Number Publication Date
JPS61159135A true JPS61159135A (en) 1986-07-18

Family

ID=17639446

Family Applications (1)

Application Number Title Priority Date Filing Date
JP59281457A Pending JPS61159135A (en) 1984-12-31 1984-12-31 Particle analyzing device

Country Status (1)

Country Link
JP (1) JPS61159135A (en)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996012172A1 (en) * 1994-10-14 1996-04-25 University Of Washington Flow cytometer jet monitor system
US5602349A (en) * 1994-10-14 1997-02-11 The University Of Washington Sample introduction system for a flow cytometer
US6248590B1 (en) 1998-02-27 2001-06-19 Cytomation, Inc. Method and apparatus for flow cytometry
US6819411B1 (en) 1997-01-31 2004-11-16 Xy, Inc. Optical apparatus
US6861265B1 (en) 1994-10-14 2005-03-01 University Of Washington Flow cytometer droplet formation system
US7723116B2 (en) 2003-05-15 2010-05-25 Xy, Inc. Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
US7758811B2 (en) 2003-03-28 2010-07-20 Inguran, Llc System for analyzing particles using multiple flow cytometry units
US7772005B1 (en) 1998-07-30 2010-08-10 Xy, Llc Method of establishing an equine artificial insemination sample
US7838210B2 (en) 2004-03-29 2010-11-23 Inguran, LLC. Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations
US7855078B2 (en) 2002-08-15 2010-12-21 Xy, Llc High resolution flow cytometer
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
JP2013513109A (en) * 2009-12-04 2013-04-18 ライフ テクノロジーズ コーポレーション Apparatus, system, method and computer readable medium for acoustic flow cytometry
US9134220B2 (en) 2004-07-27 2015-09-15 Beckman Coulter, Inc. Enhancing flow cytometry discrimination with geometric transformation
US9145590B2 (en) 2000-05-09 2015-09-29 Xy, Llc Methods and apparatus for high purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa
US9422523B2 (en) 1997-12-31 2016-08-23 Xy, Llc System and method for sorting cells
US9879221B2 (en) 2000-11-29 2018-01-30 Xy, Llc Method of in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
KR20190086272A (en) * 2018-01-12 2019-07-22 주식회사 지티에스엠 Wafer-type particle sensor
US11230695B2 (en) 2002-09-13 2022-01-25 Xy, Llc Sperm cell processing and preservation systems

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5876740A (en) * 1981-10-15 1983-05-09 インタ−ナシヨナル・リモ−ト・イメ−ジング・システムズ Method of analyzing grain of dilute liquid sample

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5876740A (en) * 1981-10-15 1983-05-09 インタ−ナシヨナル・リモ−ト・イメ−ジング・システムズ Method of analyzing grain of dilute liquid sample

Cited By (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6861265B1 (en) 1994-10-14 2005-03-01 University Of Washington Flow cytometer droplet formation system
US5602039A (en) * 1994-10-14 1997-02-11 The University Of Washington Flow cytometer jet monitor system
US5602349A (en) * 1994-10-14 1997-02-11 The University Of Washington Sample introduction system for a flow cytometer
US5726364A (en) * 1994-10-14 1998-03-10 The University Of Washington Sample introduction apparatus for a flow cytometer
AU691693B2 (en) * 1994-10-14 1998-05-21 University Of Washington Flow cytometer jet monitor system
WO1996012172A1 (en) * 1994-10-14 1996-04-25 University Of Washington Flow cytometer jet monitor system
US7923252B2 (en) 1994-10-14 2011-04-12 University Of Washington Droplet formation systems for flow cytometers
US7929137B2 (en) 1997-01-31 2011-04-19 Xy, Llc Optical apparatus
US6819411B1 (en) 1997-01-31 2004-11-16 Xy, Inc. Optical apparatus
US9422523B2 (en) 1997-12-31 2016-08-23 Xy, Llc System and method for sorting cells
US6248590B1 (en) 1998-02-27 2001-06-19 Cytomation, Inc. Method and apparatus for flow cytometry
US6589792B1 (en) 1998-02-27 2003-07-08 Dakocytomation Denmark A/S Method and apparatus for flow cytometry
US7772005B1 (en) 1998-07-30 2010-08-10 Xy, Llc Method of establishing an equine artificial insemination sample
US9145590B2 (en) 2000-05-09 2015-09-29 Xy, Llc Methods and apparatus for high purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa
US9879221B2 (en) 2000-11-29 2018-01-30 Xy, Llc Method of in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
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