JPS61148343A - Drying of blood smear sample - Google Patents

Abstract

PURPOSE:To enable the drying of a sample within a fixed range of corpuscle size, by turning a slide glass at the revolutions lower than those at the smearing after the end of smearing blood to dry up the smeared surface. CONSTITUTION:A slide glass 1 is placed on a rotary base 2, vein blood containing an anticoagulant is dropped on the glass 1 and a lid 6 is closed. Upon the closing of the lid 6, the rotary base 2 turns to smear blood over the entire surface of the glass 1. After the end of the smearing, the glass 1 is turned at the revolutions lower than those at the smearing to dry up the smeared surface. By this method, drying can be done within a centrifugal type smearer only by a rotating force without use of ant drying fan. Moreover, a sample drying is made possible within a fixed range area of corpuscle size.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a blood smear drying mechanism suitable for a drying method for preparing a blood smear, a preservation method, and automatic classification of blood images.

[Background of the invention]

Blood smears must be dried immediately after smearing.

Generally, most smeared specimens are dried using a drying bed machine such as a fan or dryer installed separately.

As described in Japanese Patent Application Laid-Open No. 56-55579, there is a device in which a smear device and a drying device are integrated to facilitate the drying process, but due to the structure of this device, the smeared specimen cannot be dried in seven units. )K transfer work is required. In addition, the size of the blood cells is greatly affected by indoor temperature, humidity, wind flow, etc., and even if the air volume is kept constant using the adjustment knob, the size of the blood cells may fluctuate significantly.

In addition, especially in the case of a centrifugal smear device, the slide glass
Since blood is painted on the surface, the specimen must be removed using tweezers, gloves, etc., making the operation even more complicated.

[Purpose of the invention]

An object of the present invention is to provide a drying method that allows a blood smear to be dried inside a centrifugal smear device, thereby producing a specimen with a uniform blood cell size within a certain range without being affected by room temperature, humidity, etc. There is a particular thing.

[Summary of the invention]

In a centrifugal smear device that smears blood by placing a slide glass on a blood tray housed in a sealed case and mounted on the rotating shaft of a drive motor, the slide glass is rotated. The method is characterized in that the smeared surface is dried by rotating the slide glass at a lower rotational speed.

That is, in the present invention, after a blood smear is prepared using a centrifugal smear device, the smear is dried by rotating it at a lower rotation speed for several seconds. By keeping the size within a certain range, a specimen suitable for use in an automatic blood image classification device can be obtained.

[Embodiments of the invention]

Next, embodiments of the present invention will be described based on the drawings.

FIG. 1 shows a cross-sectional view of the structure of a centrifugal smearing device for carrying out the present invention. As shown in FIG. 1, a lid 6 is pivotally supported on the case 5 so that it can be opened and closed.
N-01? It is supposed to be F. A drive motor 3 is provided in the case 5 with its rotating shaft oriented vertically, and a rotating table 2 is provided at the upper end of the rotating shaft. code 4
is a blood tray for receiving blood scattered during rotation. An air filter 7 is provided below the case 5 along with an opening, and the air that has passed through this is forcedly blown onto the blood by a fan 8. Reference numeral 1 is a slide glass for making a blood smear. 10 is a control circuit;
When the microswitch 11 is turned on, the drive motor is rotated at a predetermined rotation speed, and after the smearing is completed, a control signal is output to automatically switch to low speed rotation for drying rotation.

In the above configuration, the drying method according to the present invention will be explained below.

First, the sulfide glass 1 is placed on the turntable 2, 200 μt of venous blood containing an anticoagulant is dropped near the center of the slide glass 1, and the lid 6 is closed. When the lid 6 is closed, the motor 3 is driven and the turntable 2 is rotated, and the blood is spread radially and smeared onto the surface of the slide glass 1.

Usually, the rotation speed for smearing is about 500 Or, and the rotation time for p, m is 1.0 to 1.5 seconds.

As an index to determine the optimal conditions for drying, the number of pixels of the nuclear area of white blood cells (lobulated neutrophils) (one pixel is 20.23 μm)
was used. The optimum number of pixels for stably classifying blood cells is 1.100 to 1,300.

Figure 2 shows the change in image *a due to an increase in the number of revolutions.

It has been confirmed through experiments that in order to obtain a pixel count of i, too, a voltage of 6 V or more and a rotation speed of 1.30 Or, p, m or more are required.

Furthermore, the following experiment was conducted regarding the rotation time for drying. Figure 3 shows that after smearing, the rotation speed was 2.80Or;
Dry by rotating at p, m for 5.10, 15.20 seconds, remove from the apparatus, and dry by force or naturally. When the device was rotated for 15 to 20 seconds, there was no difference between forced drying and natural drying, and it was confirmed that drying the inside of the device alone was sufficient.

Figure 4 shows rotation speed 2.80Or, p, m drying rotation time 1
White blood cells (lobulated neutrophils) when drying 50 samples in 0 seconds
This is the result of measuring the number of pixels of the nuclear area.

Optimal number of pixels for automatic blood image classification: 1,100-1,300
It was confirmed that all 50 samples were received during this period and that the drying conditions were constant.

As a result, in the prior art, a dryer is installed separately,
The smear specimen must be removed from the smear device and placed in the dryer, and even with a constant air flow of 77 mph, the room temperature,
The size of blood cells varied considerably due to the influence of humidity, etc., but with this invention, the stems can be dried using only rotational force inside the centrifugal smear device without using a drying fan, and the size of blood cells can be reduced. It became possible to dry specimens within a certain range.

By using the above method, it is possible to dry blood smears inside the centrifugal smear device, and the specimens are not affected by room temperature, humidity, etc., and are suitable for use in automatic blood image classification devices. can get.

Furthermore, this drying method is economical as it does not require the addition of a new hardware mechanism.

Additionally, the blood smear is removed after it has completely dried, which helps prevent infection.

〔Effect of the invention〕

As described above, according to the present invention, a blood smear sample is dried inside a centrifugal smear device, and a sample with a uniform blood cell size within a certain range can be prepared without being affected by room temperature, humidity, etc. can do.

[Brief explanation of drawings]

Figure 1 is a cross-sectional view of a centrifugal smear device for carrying out the present invention, Figure 2 is a characteristic diagram showing the relationship between drying rotation speed and neutrophil contact area, and Figure 3 is a diagram showing the relationship between drying rotation time and preferred FIG. 4 is a characteristic diagram showing the relationship between the neutrophil contact area and the variation in the neutrophil contact area due to multiple samples. DESCRIPTION OF SYMBOLS 1... Slide glass, 2... Turntable, 3... Drive motor, 4... Blood receiver, 5... Case, 6...
・Lid, 7... Air filter, 8... Fan, 10.
...Control unit, 11...Group 'L8: 1 oz A 6 f 10
tt IQ-Voltage (V) 3

Claims (1)

[Claims] 1. In a centrifugal smear device that smears blood by placing a slide glass on a blood tray housed in a sealed case and mounted on the rotating shaft of a drive motor and rotating the slide glass, after smearing is completed, at the time of smearing. A method for drying a blood smear specimen, the method comprising: drying the smear surface by rotating the slide glass at a rotation speed lower than the rotation speed of the slide glass.