JPS61133842A - Evaluation for quality of large sea weed - Google Patents
Evaluation for quality of large sea weedInfo
- Publication number
- JPS61133842A JPS61133842A JP59256361A JP25636184A JPS61133842A JP S61133842 A JPS61133842 A JP S61133842A JP 59256361 A JP59256361 A JP 59256361A JP 25636184 A JP25636184 A JP 25636184A JP S61133842 A JPS61133842 A JP S61133842A
- Authority
- JP
- Japan
- Prior art keywords
- quality
- fluorescence
- intensity
- curve
- evaluation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N2021/635—Photosynthetic material analysis, e.g. chrorophyll
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、大型藻類の品質の評価方法に関するものであ
る。詳しくは１本発明は大型藻類の色素活性を螢光光度
法を用いて測定し、非破壊的にその品質を評価する万ε
に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for evaluating the quality of macroalgae. In detail, 1. The present invention is a method for measuring the pigment activity of macroalgae using fluorophotometry and non-destructively evaluating its quality.
It is related to.
従来、大型藻類の色素含量がその品質と密接な関係にあ
ることは公知であり、その際１色素含量の測定は、藻類
の試料片をホモジナイズし。It has been known that the pigment content of macroalgae is closely related to its quality, and the pigment content can be measured by homogenizing a sample piece of algae.
色素を抽出した後、色素の吸収スペクトル或は螢光スペ
クトルを測定することによって行われていた。This was done by extracting the dye and then measuring the absorption spectrum or fluorescence spectrum of the dye.
しかしながら、試料片のホモジナイズ、色素の抽出は操
作が繁雑であるばかりでなく、時間も要するので、より
簡便な方法で大型藻類の色素含量を測定し、その品質を
評価する万去が強く望まれている。However, homogenizing sample pieces and extracting pigments are not only complicated operations but also time-consuming, so there is a strong desire for a simpler method to measure the pigment content of macroalgae and evaluate its quality. ing.
本発明は大型藻類の品質を正確にしかも短時間で簡単に
評価できる方法を提供しようとするものである。The present invention aims to provide a method for easily evaluating the quality of macroalgae accurately and in a short period of time.
本発明は、大型藻類の試料片に励起光を照射してその螢
−ｙｔ、強度を測定し、得られた測定値を予め設定され
た螢光強度一品質検定曲線に照合し１品質を評価するこ
とよりなる大型藻類の品質評価方圧を要旨とするもので
ある。The present invention measures the fluorescence intensity by irradiating excitation light onto a sample piece of macroalgae, and compares the obtained measurement value with a preset fluorescence intensity-quality test curve to evaluate the quality. This article summarizes the methods for evaluating the quality of macroalgae.
以下１本発明を更に詳細に説明する。The present invention will be explained in more detail below.
本発明方法における大型藻類は１、アオナ目。The macroalgae used in the method of the present invention are 1, Aonidae.
ミハ目等の緑藻類、′ア゛ミジクサ目、力カモノリ目−
コンブ目−ヒバマタ目等の褐藻類、ウシケノリ目、テン
グサ目、スギノリ目等の紅藻類などの大型で、かつ植物
色素を持つ藻類である。Green algae such as the order Mihaliformes;
They are large algae that have plant pigments, such as brown algae of the order Laminata - order Fucus, and red algae of the orders Order Laminaria, Order Amanita, and Order Order Laminaria.
本発明において藻類の螢光強度を測定するにすればよい
。In the present invention, the fluorescence intensity of algae may be measured.
測定に使用する装置としては、この種の測定に一般に用
いられているものから選ばれ１通常螢光光度計又は顕微
鏡光度計が使用される。The apparatus used for the measurement is selected from those commonly used for this type of measurement, and is usually a fluorometer or a microscope photometer.
螢元元度計で螢光強度を測定するための藻類の色素とし
ては各種のものが挙げられ、例えばクロロフィルａ、ｂ
−又はＣ−フィコエリスリン、フィコシアニン−カロチ
ノイド等が挙げられる。セし７て−これらの色素のうち
から螢光強度やこれと品質との相関関係において、その
藻類に最も効果的な色素を選び測定するのがよい。Various algae pigments can be used to measure fluorescence intensity with a fluorometer, such as chlorophyll a and b.
- or C-phycoerythrin, phycocyanin-carotenoid, and the like. From among these pigments, it is best to select and measure the most effective pigment for the algae in terms of fluorescence intensity and the correlation between this and quality.
この場合、励起光を受は取る色素と螢光を発する色素は
必ずしも同一である必要はない。In this case, the dye that receives and receives the excitation light and the dye that emits fluorescence do not necessarily have to be the same.
螢光強度は品質σ〕よい藻類のものほどその色素活性度
が高く品質に比例してほぼ直線的に高くなる。従って本
発明においては予め螢光強度−品質曲線を求めて検量線
を設定しておき、これを利用して品質を評価するのであ
る。The fluorescence intensity is determined by the quality σ] The better the algae, the higher the pigment activity, which increases almost linearly in proportion to the quality. Therefore, in the present invention, a fluorescent intensity-quality curve is determined in advance to set a calibration curve, and this is used to evaluate the quality.
なお、螢光強度−品質曲線を求める際の大型藻類グ）品
質のメイキャップは１例えば、異なる条件下に試料片を
熱処理して光合成酸素発生量の異なる試料を調整するこ
とによって行うことができる、。In addition, when determining the fluorescence intensity-quality curve, the makeup of macroalgae quality can be done by, for example, heat-treating sample pieces under different conditions to prepare samples with different amounts of photosynthetic oxygen production. ,.
次に本発明暑実施例により更に詳細に説明するが１本発
明はその要旨を超えない限り以下の実施例に限定される
ものではない。Next, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited to the following Examples unless the gist thereof is exceeded.
実施例／
試料片としての約／ｄのスサビノリ葉片を二ｃｃの培地
が入った試験管に入れる。この試験管ｙ（５ｏ℃の水浴
で夫々θ秒、／り秒、３０秒、６０秒、ノー０秒熱処理
したのちノリ葉片を螢ｉ５１計（日立フローレツセンス
スペクトロホトメータＦ、ＱＱ−８Ｊのホルダーにセン
トし。Example/A sample piece of Japanese cabbage leaf of approximately /d is placed in a test tube containing 2 cc of medium. After heat-treating this test tube y in a water bath at 5oC for θ seconds, /sec, 30 seconds, 60 seconds, and 0 seconds, the nori leaf pieces were heated using a firefly i51 meter (Hitachi Florescens Spectrophotometer F, QQ-8J). Put a cent in the holder.
３ｔｌ□ｎ、ｍの光で色素を励起して生じた螢光のうち
−ＡｇＱｎｍの光を選び螢光強度を測定する。−万、同
じ様に熱処理した夫々のノリを酸素びんに入れ、それぞ
れ−０℃で約／ ０，０００ ｌｕｘの光を二時間照射
した後の光合成酸素発生量をウィンクラ−法で測定した
。藻類の活性Ｃ品質ンを反映すると広（認められている
光合成酸素発生量と先に求めた色素の螢光強度の相関を
図示すると第１図のようになり良い相関関係が得られ１
色素の螢光強度が藻類の生体活性を反映している事が確
認された。図中−横軸には、光合成酸素発生量（１０ｇ
Ｏ２）を縦軸には、相対螢光強度を示す。Among the fluorescence produced by exciting the dye with 3tl□n,m light, -AgQnm light is selected and the fluorescence intensity is measured. - 10,000 pieces of seaweed that had been heat-treated in the same way were placed in an oxygen bottle and irradiated with light of about 0,000 lux at -0°C for 2 hours, after which the amount of photosynthetic oxygen generated was measured using the Winkler method. When the active C quality of algae is reflected, the correlation between the recognized amount of photosynthetic oxygen generated and the previously determined fluorescence intensity of the pigment is shown in Figure 1, and a good correlation is obtained.
It was confirmed that the fluorescence intensity of the dye reflected the biological activity of the algae. In the figure, the horizontal axis shows the amount of photosynthetic oxygen generated (10g
The vertical axis indicates relative fluorescence intensity.
以上のように一本発明による品質評価広では正確でしか
も簡便・短時間で測定できる上、非 ′破壊的に試料の
測定ができろため測定後の試料は再度、培養、養殖等が
できるという大きな利点を有する。As described above, the quality evaluation method according to the present invention is accurate, simple, and quick to measure, and since the sample can be measured non-destructively, the sample after measurement can be cultured, cultured, etc. again. Has great advantages.
第７図は、海苔の光合成酸素発生量（ｌｏｇｏ２）と相
対螢光強度との関係を示すグラフであり。
横軸は光合成酸素発生量を、縦軸は相対螢光強度を示す
。FIG. 7 is a graph showing the relationship between the amount of photosynthetic oxygen generated (logo2) and the relative fluorescence intensity of seaweed. The horizontal axis shows the amount of photosynthetic oxygen generated, and the vertical axis shows the relative fluorescence intensity.
Claims (1)
度を測定し、得られた測定値を予め設定された螢光強度
一品質検定曲線に照合し、品質を評価することを特徴と
する大型藻類の品質評価方法。(1) Measure the fluorescence intensity by irradiating a sample piece of macroalgae with excitation light, compare the obtained measurement value with a preset fluorescence intensity-quality test curve, and evaluate the quality. Characteristic method for evaluating the quality of macroalgae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59256361A JPS61133842A (en) | 1984-12-04 | 1984-12-04 | Evaluation for quality of large sea weed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59256361A JPS61133842A (en) | 1984-12-04 | 1984-12-04 | Evaluation for quality of large sea weed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS61133842A true JPS61133842A (en) | 1986-06-21 |
Family
ID=17291609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59256361A Pending JPS61133842A (en) | 1984-12-04 | 1984-12-04 | Evaluation for quality of large sea weed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61133842A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2005062027A1 (en) * | 2003-12-19 | 2007-07-12 | 浜松ホトニクス株式会社 | Hazardous substance evaluation method and hazardous substance evaluation kit |
| JP2015049227A (en) * | 2013-09-04 | 2015-03-16 | 独立行政法人国立高等専門学校機構 | Growth diagnostic system and method for large sized algae |
-
1984
- 1984-12-04 JP JP59256361A patent/JPS61133842A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2005062027A1 (en) * | 2003-12-19 | 2007-07-12 | 浜松ホトニクス株式会社 | Hazardous substance evaluation method and hazardous substance evaluation kit |
| JP4699214B2 (en) * | 2003-12-19 | 2011-06-08 | 浜松ホトニクス株式会社 | Hazardous substance evaluation method and hazardous substance evaluation kit |
| US9448170B2 (en) | 2003-12-19 | 2016-09-20 | Hamamatsu Photonics K.K. | Harmful substance evaluating method and harmful substance evaluation kit |
| JP2015049227A (en) * | 2013-09-04 | 2015-03-16 | 独立行政法人国立高等専門学校機構 | Growth diagnostic system and method for large sized algae |
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