JPS61128168A - Immunological analysis - Google Patents
Immunological analysisInfo
- Publication number
- JPS61128168A JPS61128168A JP24997484A JP24997484A JPS61128168A JP S61128168 A JPS61128168 A JP S61128168A JP 24997484 A JP24997484 A JP 24997484A JP 24997484 A JP24997484 A JP 24997484A JP S61128168 A JPS61128168 A JP S61128168A
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- Prior art keywords
- particle
- particles
- antigen
- antibody
- reaction mixture
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
【発明の詳細な説明】
(産業上の利用分野)
本発明は不溶性担体粒子を使用する免疫分析方法に関す
る。DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to an immunoassay method using insoluble carrier particles.
(従来の技術) 本発明は、抗原抗体反応の測定方法に関する。(Conventional technology) The present invention relates to a method for measuring antigen-antibody reactions.
従来、ラテックス等の不溶性担体粒子に担持させた抗体
又は抗原と抗原又は抗体めるいはその混合物とを液体媒
体中で反応させ、その反応の進行く伴う反混合物の透過
率の減少(すなわち吸光度の増加)からその抗原抗体反
応の速度を測定し、さらにその速度から被検体中の抗原
又は抗体の濃度を定量する方法が知られている(特公超
IF−//Jr7Jr号公報)。Conventionally, antibodies or antigens supported on insoluble carrier particles such as latex are reacted with antigens or antibodies or a mixture thereof in a liquid medium, and as the reaction progresses, the transmittance of the anti-mixture decreases (i.e., the absorbance decreases). A method is known in which the rate of the antigen-antibody reaction is measured based on the increase in the number of antigens and antibodies, and the concentration of the antigen or antibody in the subject is determined from the rate (Japanese Patent Publication No. 7, JR7Jr).
そして、この方法によれば、抗原又は抗体の濃度を高い
精度で、迅速に定量しうる。According to this method, the concentration of antigen or antibody can be determined rapidly and with high accuracy.
(発明が解決しようとする問題点)
しかしながら、この方法では、感度の点で、数n y
/d程度までしか信頼しうる測定値は得られず生長ホル
モy、インスリン、ジゴキシン等のさらに高感度の測定
を必要とする項目については、必ずしも十分に満足しう
るものではない。(Problem to be solved by the invention) However, in this method, in terms of sensitivity, the number n y
Reliable measurement values can only be obtained up to about /d, and items that require even more sensitive measurements such as growth hormone y, insulin, and digoxin are not necessarily fully satisfied.
そこで、本発明者らは、上記のような抗原抗体反応の測
定において、さらに感度を向上させるぺく、種々検討を
重ねた結果、本発明に到達し九。Therefore, the present inventors conducted various studies to further improve the sensitivity in the measurement of antigen-antibody reactions as described above, and as a result, they arrived at the present invention.
(問題点を解決するための手段)
本発明の要旨は、不溶性担体粒子に抗体又は抗原を支持
させ、この支持された抗体又は抗原に、抗原又は抗体あ
るいはその混合物を液体媒体中で反応させて、該反応の
進行く伴う反応混合物の凝集の度合を測定する方法にお
いて、上記担体粒子として磁化可能粒子と標識粒子とを
用いて反応させ、ついで反応混合物に磁場を付与するこ
とKよシ磁化した磁化粒子又は磁化粒子を含む凝集粒子
を反応混合物から分離し、この凝集粒子中の標識粒子を
、その標識によって検知することにより、反応混合物の
凝集の度合を標ml!![度の増加として測定すること
を特徴とする免疫分析方法にろる。(Means for Solving the Problems) The gist of the present invention is to support an antibody or antigen on an insoluble carrier particle, and react the supported antibody or antigen with the antigen or antibody or a mixture thereof in a liquid medium. In the method of measuring the degree of aggregation of the reaction mixture as the reaction progresses, the reaction mixture is caused to react using magnetizable particles and label particles as the carrier particles, and then a magnetic field is applied to the reaction mixture, so that the reaction mixture is magnetized. Magnetized particles or aggregated particles containing magnetized particles are separated from the reaction mixture, and labeled particles in the aggregated particles are detected by the label to determine the degree of aggregation of the reaction mixture. ! [This is an immunoassay method characterized by measuring as an increase in the degree of
以下、本発明の詳細な説明する。The present invention will be explained in detail below.
まず、本発明は、上記のとおり不溶性担体粒子に抗体又
は抗原を支持させ、この支持された抗体又は抗原に、抗
原又は抗体あるいはその混合物を液体媒体中で反応させ
て、この反応混合物の凝集の度合を測定する方法におい
て適用される。First, the present invention involves supporting an antibody or an antigen on an insoluble carrier particle as described above, reacting the supported antibody or antigen with the antigen or antibody, or a mixture thereof in a liquid medium, and causing aggregation of the reaction mixture. Applied in the method of measuring degree.
不溶性担体粒子としては、測定を行なう時に用いられる
液体媒体に実質的に不溶性で、約10μ程度以下、好ま
しくは、O,OS〜!、0μの平均粒径を有する有機高
分子物質の微粒子、たとえばポリスチレン、スチレン−
ブタジェン共重合体のような乳化重合によシ得られる有
機高分子のラテックス、おるいはシリカ、シリカ−アル
ミナ、アルミナのよう々無機酸化物等が用いられる。The insoluble carrier particles are substantially insoluble in the liquid medium used in the measurement and have a size of about 10μ or less, preferably O,OS~! , fine particles of organic polymeric substances having an average particle size of 0μ, such as polystyrene, styrene-
Organic polymer latex obtained by emulsion polymerization such as butadiene copolymer, or inorganic oxides such as silica, silica-alumina, and alumina are used.
本発明方法においてはこのような不溶性担体粒子(たと
えばラテックス粒子)に、測定しようとする被検体中の
抗原及び/又は抗体と反応しうる抗体又は抗原を担持さ
せる(感作する)。In the method of the present invention, such insoluble carrier particles (for example, latex particles) are made to carry (sensitize) an antibody or antigen that can react with the antigen and/or antibody in the analyte to be measured.
この場合、担体に対し抗体又は抗原を物理的に吸着させ
てもよいし、化学的に吸着させてもよい。抗体は蛋白質
で構成されておシ、一方抗原はたとえは蛋白質、ポリペ
プチド、ステはイド、多!S類、脂賀、花粉等種々のも
のから々る。不溶性担体粒子にこのような抗体又は抗原
を担持させる方法はすでに多くの方法が提案されている
。不溶性担体に九とえばハプテンのような不完全抗原を
担持させる場合は、その担体をたとえばカップリング剤
により化学的に変性して、その抗原を化学的に吸着させ
るのが有利である。In this case, the antibody or antigen may be physically or chemically adsorbed onto the carrier. Antibodies are made up of proteins, while antigens are made up of proteins, polypeptides, antibodies, and polypeptides. It comes from various things such as S species, Shiga, pollen, etc. Many methods have already been proposed for carrying such antibodies or antigens on insoluble carrier particles. When an insoluble carrier carries an incomplete antigen such as a hapten, it is advantageous to chemically modify the carrier, for example with a coupling agent, and chemically adsorb the antigen.
上記抗体又は抗原を感作し念不溶性担体粒子(たとえば
ラテックス粒子)の濃度が通常0.00 /重量%以上
の懸濁液、好ましくは0.0 / −/重量%程度の懸
濁液を用いる。A suspension is used to sensitize the above antibody or antigen and the concentration of insoluble carrier particles (e.g., latex particles) is usually 0.00/wt% or more, preferably about 0.0/-/wt%. .
前記感作担体を液体媒体中において、抗原又は抗体6る
いはその混合物と実質的に一定条件下で反応させる。The sensitized carrier is reacted with the antigen or antibody 6 or a mixture thereof in a liquid medium under substantially constant conditions.
本発明方法においては、上記不溶性担体粒子として、磁
化可能粒子と標識粒子の二種類が用いられる。In the method of the present invention, two types of insoluble carrier particles are used: magnetizable particles and labeled particles.
磁化可能粒子(以下、0粒子という)は、反応時に磁化
しておらず磁場を付与することにより磁化し、容易に液
体媒体から分離できる粒子を意味し、たと几ば、マグネ
タイト、フェライト等が好適に用いられる。たとえば、
この場合、マグネタイト、フェライト等を含んだラテッ
クス粒子を用いて、感作し、反応に供される。Magnetizable particles (hereinafter referred to as 0 particles) refer to particles that are not magnetized during a reaction but become magnetized by applying a magnetic field and can be easily separated from a liquid medium, such as magnetite, ferrite, etc. used for. for example,
In this case, latex particles containing magnetite, ferrite, etc. are used for sensitization and reaction.
また、標識粒子(以下、0粒子という)としては、ケイ
光色素を含む粒子、ラジオアイソトープ(R工) 11
1工等を含む粒子、ま九、有機溶媒を添加することによ
り容易に溶媒中に溶出してくるような色素、ペルオキシ
ダーゼ等の酵素などが挙げられる。In addition, as label particles (hereinafter referred to as 0 particles), particles containing fluorescent dyes, radioisotope (R engineering) 11
Examples include particles containing particles such as silver, pigments that can be easily eluted into a solvent by adding an organic solvent, and enzymes such as peroxidase.
たとえばケイ光色素を用いる場合1.不溶性担体粒子は
、たとえばラテックス重合の際に反応液にケイ光色素を
混合し、ラテックス粒子内に色素をとシ込んでいるもの
でも、ラテックス重合後、化学結合あるいは物理吸着等
を利用してラテックスを染色したものでもよい。For example, when using a fluorescent dye, 1. Insoluble carrier particles, for example, may be those in which a fluorescent dye is mixed with the reaction solution during latex polymerization and the dye is injected into the latex particles. It may be dyed.
ケイ光色素としてはローダミン系色素(たとえばローダ
ミン−4G、テトラメチルローダミン)、アクリジン系
色素(たとえばアクリジンオレンジ)その他、シアニン
系色素、希土類、エオシン、フルオレツセイン、クマリ
ン誘導体、スチルベン誘導体などが挙げられる。Examples of fluorescent dyes include rhodamine dyes (eg, rhodamine-4G, tetramethylrhodamine), acridine dyes (eg, acridine orange), cyanine dyes, rare earths, eosin, fluorescein, coumarin derivatives, stilbene derivatives, and the like.
磁化可能粒子と標識粒子の使用割合は、特に制限されな
いが1通常、等を程度が用いられる。The ratio of the magnetizable particles to the labeled particles is not particularly limited, but a ratio of about 1, etc. is usually used.
抗原抗体反応に際しては、−抗体法、二抗体法が応用で
きる。−抗体法の場合、■、0粒子に同一の抗体を感作
し、抗原を含む溶液中に緩衝液と共に添加すると、抗原
抗体反応によシ粒子の凝集がおこり、0粒子と0粒子と
が同時に反応することにより■−■、■−■、■−■や
これらの組み合わさった凝集がおこる。一定時間インキ
エベートした後反応混合物に磁場を与えることによシ■
粒子わるいは0粒子を含む凝集粒子を反応混合物から容
易に分離することができる。For antigen-antibody reactions, -antibody method and double antibody method can be applied. - In the case of the antibody method, (1) When 0 particles are sensitized with the same antibody and added together with a buffer into a solution containing an antigen, the antigen-antibody reaction causes aggregation of the 2 particles, and the 0 particles are separated from each other. Simultaneous reactions cause agglomeration of ■-■, ■-■, ■-■, or a combination of these. By applying a magnetic field to the reaction mixture after incubation for a certain period of time,
Agglomerated particles, including little or no particles, can be easily separated from the reaction mixture.
二抗体法の場合、たと、?、ばbる抗原(Oとする)に
対して反応する2種の抗体を゛用い、0粒子、0粒子に
それぞれ異なる抗体を感作し抗原と反応させることによ
り■−0.■−〇−■、〇−■なる粒子を得る。一定時
筒抜の反応液に磁場を与えることによシ■粒子又は0粒
子と0粒子が凝集したものを容易に分離することができ
る。In the case of the two-antibody method, what? , by using two types of antibodies that react with each antigen (denoted as O), by sensitizing 0 particles and 0 particles with different antibodies and allowing them to react with the antigen, ■-0. ■−〇−■, 〇−■ particles are obtained. By applying a magnetic field to the reaction solution that has been removed from the tube for a certain period of time, it is possible to easily separate 2 particles or 0 particles and aggregates of 0 particles.
これらの場合、反応容器の底に0粒子又は0粒子と0粒
子が凝集したものを集めて上清液を除去し、残った粒子
中の標識物を測定すると、反応液中の抗原濃度に比例し
た標識物が観察される。In these cases, if you collect 0 particles or aggregates of 0 particles and 0 particles at the bottom of the reaction container, remove the supernatant, and measure the labeled substance in the remaining particles, it will be proportional to the antigen concentration in the reaction solution. A labeled substance is observed.
標識物の測定には、たとえばケイ光色素の場合、励起光
及びケイ光を吸収しないような反応容器の底に集めた後
、容器の底から励起光を当てて出てくるケイ光を11定
する系を用いると。To measure a labeled substance, for example, in the case of a fluorescent dye, it is collected at the bottom of a reaction vessel that does not absorb excitation light and fluorescence, and then the excitation light is irradiated from the bottom of the vessel and the fluorescent light that comes out is quantified for 11 seconds. If you use a system that
上清の除去は不要とをる。ま光、上清を除去した後、ア
ルコール等の有機溶媒を添加して、粒子から色素の溶出
を訃こなわせると、よシ高感度でff度の高い測定とな
る。There is no need to remove the supernatant. After removing the supernatant, an organic solvent such as alcohol is added to elute the dye from the particles, resulting in a highly sensitive measurement with a high degree of FF.
また、たとえば標識粒子としてケイ光色素を含んだラテ
ックスを用いフローセルを用いる測定法として、フロー
七ルの横方向からa#ht−与え、フローセルを流れる
反応液中の0粒子あるいは0粒子を含む凝集粒子が、磁
力により横方向にひきよせもれ之ところを、充分小さく
絞った励起光を当てることにより0粒子との凝集により
引きよせもれたケイ光粒子の数の増加を、度応液中の抗
原#度の増加と関連させることができる。In addition, for example, as a measurement method using a flow cell using latex containing a fluorescent dye as a label particle, a#ht- is applied from the lateral direction of the flow cell, and 0 particles or agglomerates containing 0 particles in the reaction solution flowing through the flow cell. By applying sufficiently focused excitation light to the areas where particles are attracted and leaked in the horizontal direction due to magnetic force, it is possible to increase the number of fluorescent particles that are attracted and leaked due to aggregation with zero particles. It can be associated with an increase in antigenicity.
測定は、通常反応開始後一定時間イン中ユペートシた後
行なわれる。Measurements are usually carried out after a certain period of incubation after the start of the reaction.
上記の方法により、反応混合物の凝集の度合を、一定時
間後の標識強度の増加として測定し、予め濃度既知の試
料によシ作製した抗原又は抗体の検量線を用いて、検体
中の抗体又は抗原の濃度を測定することができる。By the above method, the degree of agglutination of the reaction mixture is measured as an increase in label intensity after a certain period of time, and a calibration curve of antigen or antibody prepared in advance with samples with known concentrations is used to determine the degree of agglutination of the antibody or antibody in the sample. The concentration of antigen can be measured.
(発明の効果)
本発明方法によれば、ラテックスを用いる抗原−抗体反
応により反応液中の共存物質に影響されない高精度で、
高感度な抗原又は抗体の定量が可能でめる。(Effects of the Invention) According to the method of the present invention, an antigen-antibody reaction using latex can be performed with high precision without being affected by coexisting substances in the reaction solution.
Highly sensitive antigen or antibody quantification is possible.
(実施例) 以下、実施例により、本発明をさらに詳細く説明する。(Example) Hereinafter, the present invention will be explained in more detail with reference to Examples.
実施例/
平均粒径/μmのS性うテククス(ローヌプーラン社製
)と、Dov ChemiCe−1社fi/、/amの
ラテックスにテトラメチルローダミンを染色した(ラテ
ックス/klに対し染料/ mmolを使用)ケイ光性
ラテックスにそれぞれ抗−API’抗体を感作し1種々
の濃度のニーフェトプロティン(AFF)溶液に緩衝液
とこれら二種のラテックス(等量)を添加しく 0.O
7重量%)。Example / S-based caries (manufactured by Rhone-Poulenc) with an average particle diameter of / μm and latex of Dov ChemiCe-1 fi/, /am were dyed with tetramethylrhodamine (dye/mmol per latex/kl). Use) Sensitize each fluorescent latex with an anti-API' antibody and add the buffer and these two types of latex (equal volumes) to neifetoprotein (AFF) solutions at various concentrations. O
7% by weight).
一定時間抜細胞培養用フェル(内径7111!、深さ/
an )に一定量移し、下に磁石をおいて、フェルの
底に■−粒子及び0粒子を含む凝集粒子を集める。その
後上清を除去し、残ったケイ光粒子と0粒子にアルゴン
イオンレーザ−の!lμ、jnmの励起光を当て、ケイ
光性粒子から発せられるケイ光の強度を測定した。毎秒
カウントされるフォトン数をAFP濃度に対してプロッ
トしたものが図1である(検を線)。Fell for cell culture after a certain period of time (inner diameter 7111!, depth/
A fixed amount of the particles is transferred to an ), a magnet is placed below, and the aggregated particles including ■- particles and 0 particles are collected at the bottom of the felt. After that, the supernatant was removed, and the remaining fluorescent particles and zero particles were treated with an argon ion laser! The intensity of fluorescence emitted from the fluorescent particles was measured by applying excitation light of lμ, jnm. FIG. 1 shows the number of photons counted per second plotted against the AFP concentration (the line represents the measurement).
この検量線を用いて、AFF濃度未知の検体中の抗原濃
度を高感度で測定することができる。Using this calibration curve, the antigen concentration in a sample with unknown AFF concentration can be measured with high sensitivity.
図1は、実施例1で得られるAFPについての検1線を
示す。
出 願 人 三菱化成工業株式会社
代 理 人 弁理士 憂苦用 −ほか1名
図1
AFP濃度輛ムL)FIG. 1 shows the first line for AFP obtained in Example 1. Applicant: Mitsubishi Chemical Industries, Ltd. Agent: Patent attorney, Yukuyo - and 1 other person Figure 1 AFP concentration range L)
Claims (1)
支持された抗体又は抗原に、抗原又は抗体あるいはその
混合物を液体媒体中で反応させて、該反応の進行に伴う
反応混合物の凝集の度合を測定する方法において、上記
担体粒子として磁化可能粒子と標識粒子とを用いて反応
を行い、ついで反応混合物に磁場を付与することにより
磁化した磁化粒子又は磁化粒子を含む凝集粒子を反応混
合物から分離し、この凝集粒子中の標識粒子をその標識
によって検知することにより、反応混合物の凝集の度合
を標識強度の増加として測定することを特徴とする免疫
分析方法。(1) An antibody or antigen is supported on insoluble carrier particles, and the supported antibody or antigen is reacted with the antigen or antibody or a mixture thereof in a liquid medium, and the degree of aggregation of the reaction mixture is determined as the reaction progresses. In this method, a reaction is performed using magnetizable particles and labeled particles as the carrier particles, and then a magnetic field is applied to the reaction mixture to separate magnetized particles or aggregated particles containing the magnetized particles from the reaction mixture. and detecting labeled particles in the aggregated particles using the label, thereby measuring the degree of aggregation of the reaction mixture as an increase in label intensity.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP24997484A JPS61128168A (en) | 1984-11-27 | 1984-11-27 | Immunological analysis |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP24997484A JPS61128168A (en) | 1984-11-27 | 1984-11-27 | Immunological analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS61128168A true JPS61128168A (en) | 1986-06-16 |
Family
ID=17200953
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP24997484A Pending JPS61128168A (en) | 1984-11-27 | 1984-11-27 | Immunological analysis |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS61128168A (en) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988002118A1 (en) * | 1986-09-22 | 1988-03-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor |
JPS63108265A (en) * | 1986-10-25 | 1988-05-13 | Nippon Telegr & Teleph Corp <Ntt> | Magnetic immune measurement method by using laser |
JPS63132167A (en) * | 1987-09-11 | 1988-06-04 | Tokuyama Soda Co Ltd | Reagent for immune diagnosis |
WO1989001161A1 (en) * | 1987-07-28 | 1989-02-09 | International Institute Of Cellular & Molecular Pa | Turbidimetric assay |
JPH01193647A (en) * | 1988-01-29 | 1989-08-03 | Mitsubishi Kasei Corp | Measurement of antigen and antibody |
EP0351857A2 (en) * | 1988-07-20 | 1990-01-24 | Olympus Optical Co., Ltd. | Immunoassay method using magnetic marker particles |
JPH02151766A (en) * | 1988-12-02 | 1990-06-11 | Nippon Telegr & Teleph Corp <Ntt> | Measurement of laser magnetic immunity |
EP0417301A1 (en) * | 1989-02-10 | 1991-03-20 | Shino-Test Corporation | Method for assaying indirect agglutination |
JPH03123861A (en) * | 1989-10-06 | 1991-05-27 | Mitsubishi Kasei Corp | Immunochemical measurement method of haptens |
US5236824A (en) * | 1988-04-26 | 1993-08-17 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor |
US5238810A (en) * | 1986-09-22 | 1993-08-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus thereof |
US8008379B2 (en) | 2005-11-02 | 2011-08-30 | Fujifilm Corporation | Fluorescent polymer fine particle, method for forming thereof, fluorescence detection kit, and method for detecting fluorescence |
-
1984
- 1984-11-27 JP JP24997484A patent/JPS61128168A/en active Pending
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5238810A (en) * | 1986-09-22 | 1993-08-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus thereof |
WO1988002118A1 (en) * | 1986-09-22 | 1988-03-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor |
US5252493A (en) * | 1986-09-22 | 1993-10-12 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor |
JPS63108265A (en) * | 1986-10-25 | 1988-05-13 | Nippon Telegr & Teleph Corp <Ntt> | Magnetic immune measurement method by using laser |
JP2502546B2 (en) * | 1986-10-25 | 1996-05-29 | 日本電信電話株式会社 | Laser magnetic immunoassay method |
WO1989001161A1 (en) * | 1987-07-28 | 1989-02-09 | International Institute Of Cellular & Molecular Pa | Turbidimetric assay |
JPS63132167A (en) * | 1987-09-11 | 1988-06-04 | Tokuyama Soda Co Ltd | Reagent for immune diagnosis |
JPH01193647A (en) * | 1988-01-29 | 1989-08-03 | Mitsubishi Kasei Corp | Measurement of antigen and antibody |
WO1989007270A1 (en) * | 1988-01-29 | 1989-08-10 | Mitsubishi Kasei Corporation | Method for assaying antigen or antibody |
US5236824A (en) * | 1988-04-26 | 1993-08-17 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor |
EP0351857A2 (en) * | 1988-07-20 | 1990-01-24 | Olympus Optical Co., Ltd. | Immunoassay method using magnetic marker particles |
JPH02151766A (en) * | 1988-12-02 | 1990-06-11 | Nippon Telegr & Teleph Corp <Ntt> | Measurement of laser magnetic immunity |
EP0417301A1 (en) * | 1989-02-10 | 1991-03-20 | Shino-Test Corporation | Method for assaying indirect agglutination |
EP0417301A4 (en) * | 1989-02-10 | 1992-01-15 | Shino-Test Corporation | Method for assaying indirect agglutination |
JPH03123861A (en) * | 1989-10-06 | 1991-05-27 | Mitsubishi Kasei Corp | Immunochemical measurement method of haptens |
US8008379B2 (en) | 2005-11-02 | 2011-08-30 | Fujifilm Corporation | Fluorescent polymer fine particle, method for forming thereof, fluorescence detection kit, and method for detecting fluorescence |
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