JPS61112027A - Carcinostatic agent - Google Patents

Abstract

PURPOSE:JUZEN-TAIHO-TO (a Chinese herb tea) composed of OUGI (root of Astragalus huantchy), KEIHI (bark of Cinnamomum cassia), JIOU (root of Rehmannia glutinosa), SHAKUYAKU (root of Paeonia albiflora), SENKYU (rhizome of Cnidium officinale), JUTSU (rhizome of Atractylodes lancea), TOUKI (root of Angelica acutiloba), NINJIN (rhizome of Panax ginseng), BUKURYO (fungi of Pachyma hoelen) and KANZO (root of Glycyrrhiza glabra) is used as a carcinostatic agent free from side effects. CONSTITUTION:JUZEN-TAIHO-TO is used as a carcinostatic agent. It has been found that JUZEN-TAIHO-TO used as a Chinese herb tea for the diseases such as inappetence, fatigue and the development of physical strength after disease, etc., has carcinostatic effect. For example, 2.5-3pts. of OUGI, 3pts. of KEIHI, 3pts. of JIOU, 3pts. of SHAKUYAKU, 3pts. of SENKYU, 3pts. of JUTSU, 3pts. of TOUKI, 2.5-3pts. of NINJIN, 3pts. of BUKURYO and 1.5pts. of KANZO are extracted with 10 times weight of hot water, and the extract liquid is filtered and dried to obtain a dried extract powder of JUZEN-TAIHO- TO, which can be formed to tablet, capsule, etc., by conventional method. The agent increases the survival ratio of ICR male mouse of Ehrlich ascites carcinoma. Furthermore, it has extremely low toxicity and high safety.

Description

[Detailed description of the invention] Industrial applications The present invention relates to anticancer agents.

2. Prior Art The pharmacological mechanisms of the anticancer drugs currently in use are well defined, and many of them have excellent anticancer effects, but on the other hand, they often act on normal cells and cause fatal side effects. Therefore, it is desired to develop an anticancer agent with less i+1 action.

Problems to be Solved by the Invention The present invention provides an anticancer agent with few side effects.

Means to Solve the Problem The present inventors conducted research on the anticancer effects of various Chinese herbal prescriptions, and found that they were found to be effective against cancer. We have discovered that a Chinese herbal formula made from licorice, namely Juzentaihoto, has anticancer effects. The present invention is based on this knowledge, and is an anticancer agent comprising Juzentaihoto. Juzentaihoto's constituent herbal medicines, dosage, extraction method, etc. are described in the classic Chinese herbal prescription (Japanese Pharmacopoeia), and it is used to treat anorexia, fatigue, fatigue, and increase physical strength after illness. It was previously unknown that it had anticancer effects.

Juzendai? 1ItIIh follows the classics and buys 3. Og,
Cinnamon bark 3, Og, rhizome 30g, Chinese herbal medicine 3. Og, bow 3゜o
g, bend 3. Og, return 3. Og, carrot 3.0g, Kessei 3
．． Og, boil 1.5g of licorice with 600cc of water for 350
It is possible to remove the residue from the cc and take it as an anticancer drug in three doses, but it is also possible to use a Chinese herbal medicine extract preparation as an anticancer drug for ease of administration and portability.

For example, buy 2.5 to 3 parts by weight and cinnamon 3 parts by weight.

3 parts by weight of Rhizoma, 3 parts by weight of Xuyaku, 3 parts by weight of Yōkyu, 3 parts by weight of Mixture, 3 parts by weight of Danggui, 2.5-3 parts by weight of Ginseng, 3 parts by weight of Keili, 1.5 parts by weight of Licorice. The extracted liquid obtained by hot extraction with 10 times the amount of water is dried to obtain Juzentaihoto dry extract powder, which is an anticancer agent, and suitable excipients used in ordinary preparations are added to this powder. , adjuvants, etc. can be added to form preparations such as powders, granules, tablets, and capsules according to conventional methods for manufacturing preparations.

A specific example of the production of the anticancer agent of the present invention is as follows.

Concrete example! Precious 3. Og, cinnamon 3.0g, rhizome 3. OgS medicine 3.
Og, 153.0g, 3 seeds. Og, Toki 3゜Og, Ginseng 3.0g, Kessai 3. Add 10 times the amount of water (285 -
Extraction was carried out by heating at .degree. C., and the resulting extract was filtered and spray-dried to obtain 2.2 g of dry extract powder.

Effects of the Invention The anticancer effect of the anticancer agent of the present invention will be explained by giving experimental examples.

Experimental example ■Animal used: ICn strain male mouse.

(2) Tumor cells + Ehrlich ascites cancer cells whose tumors were passaged in ICR mice were used.

■Experimental method - Tumor cells were collected from mice 8 days after transplantation of Ehrlich fil (referred to as EAC), and 2×10 cells 10. :2t
The anticancer agent of the present invention obtained in Example 1 was diluted with physiological saline to give a concentration of 100, 200 and 300 mg after 24 hours.
The drug was dissolved in physiological saline and administered intraperitoneally for 18 indigo and lθ days, and survival days and survival rate increase and anticancer effect were determined. As a control, physiological saline containing no anticancer drug of the present invention was administered at 0.25ml/kg per day! It was administered intraperitoneally. The results are shown in Table 1.

The numbers below the mean survival days in Table 1 indicate the standard error of the mean.

As is clear from Table 1, the anticancer agent of the present invention increased the survival rate of mice, and an anticancer effect was observed.

Next, an acute toxicity test for oral administration of the anticancer agent of the present invention was conducted on d
dY male mice and Wistar (W 1star)
When tested using male rats, the anticancer agent of the present invention obtained in Example 1 did not cause death even when administered orally at a dose of 15 g/kg (dose limit).

Thus, the anticancer agent of the present invention has extremely low toxicity and high safety. Furthermore, since Juzentaihoto has been used as a Chinese herbal medicine since ancient times to the present, it can be seen that it has few side effects.

Considering the experimental data and the results of acute toxicity tests in the present invention, the effective dose of the anticancer agent of the present invention is determined by the age of the patient,
Although it varies depending on body weight and degree of disease, it is usually used as an adult amount as a dry extract powder amount! It is considered appropriate to take a daily dose of 1 to 10 g divided into three times a day depending on the symptoms.

Next, examples will be shown and specifically explained, but the present invention is not limited thereto.

Example! 200g of the drug produced according to the above specific example 1 was added to 89g of lactose.
g and magnesium stearate Ig, and this mixture was compressed using a single-shot tablet machine to form tablets with a diameter of 20 mm and a weight of Ig.
Approximately 2.3 g of slug tablets were prepared, pulverized with an oscillator, sized, and sieved to obtain good granules of 20 to 50 mesh particles.

This granule is available in an equal amount of 0.5 to 45 g (
The dry extract powder weight of the anticancer agent of the present invention is 0.34~
3. (equivalent to lOg) three times a day.

Example 2 200 g of the drug produced according to Example 1 above was mixed with 20 g of microcrystalline cellulose and 5 g of magnesium stearate, and this mixture was compressed into tablets with a single-shot key press to form tablets with a diameter of 7.
A tablet having 1 cocoon and a weight of 225B was produced. Book tablets! The solution contains 200 mg of dry extract powder of the anticancer agent of the present invention. Example 3 The tablets are taken in doses of 2 to 16 tablets three times a day depending on the symptoms. 500 mg of the drug produced in Example 1 above was filled into hard capsules. This capsule takes 2 to 2 doses depending on the symptoms.
Take o capsules in 3 divided doses a day.

Claims (1)

[Claims] Anticancer drug made from Juzentaihoto