JPS61100184A - Health food - Google Patents

Abstract

PURPOSE:To provide a health food effective to decrease the cholesterol level in blood, increase the body weight and suppress the deposition of neutral fat in liver and the deposition of fat to the body, and containing a processed non- volatile salt prepared by neutralizing an unrefined SAKE or vinegar, etc. prepared by acetic acid fermentation with a basic calcium-containing substance, etc. CONSTITUTION:Unrefined SAKE or vinegar prepared by acetic acid fermentation or its separation residue is neutralized with a basic or neutral calcium-containing substance (e.g. calcium hydroxide) to neutralize acidic volatile components to non-volatile components. The treated product is used as a component of the objective health food. The product may be used as it is, or used after the defatting, concentration, drying or pulverization treatment.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a health food whose active ingredients are mash obtained by acetic acid fermentation, vinegar, or a processed product of vinegar separation residue.

[Conventional technology]

Vinegar contains various volatile organic acids, amino acids, esters, and sugars in addition to acetic acid, and has a unique flavor derived from the raw materials as well as aromas and umami produced during the fermentation process, making it an important dietary seasoning. occupying a position. It is also known that drinking it is effective for improving health.

However, it has not been easy to drink vinegar as it is due to its strong sour taste. On the other hand, although calcium is one of the nutrients necessary for the human body, its existing form in nature is mainly an inorganic salt, which is not a suitable form for intake.

As a result of extensive research into improving the taste and utilization of mash, vinegar, or vinegar separation residue obtained from acetic acid fermentation, the inventor found that by neutralizing these with a basic or neutral calcium-containing substance, 1 vinegar and calcium They discovered that a food product suitable for consumption that has a health-promoting effect based on the coexistence of the following ingredients was completed, and this invention was completed.

[Structure of the invention]

In other words, the present invention involves treating mash, vinegar, or the separated residue obtained by acetic acid fermentation with a basic or neutral calcium-containing substance to neutralize acidic volatile components and produce a non-volatile salt. include.

It is a health food.

The moromi, vinegar or its separated residue used in this invention is
It may be any product obtained by acetic acid fermentation. Namely, alcoholic vinegar (made from alcohol made from sweet potato, potato, precision, pulp waste liquid, etc.), rice vinegar (made from rice malt), lees vinegar (made from sake lees), and malt vinegar (made from rice koji). known acetic acid fermentation to obtain apple cider vinegar, grape vinegar, etc. (made from alcohol made from grain-based alcohol), apple cider vinegar, grape vinegar, etc.
A. xylinum, A. acetosus, A. aceti, A. quetingianus, A. ascendens, A. schellaen bee, etc. Moromi 1 vinegar or vinegar separation residue is used.

In addition, as basic or neutral calcium-containing substances,
Calcium hydroxide, calcium carbonate, calcium phosphate,
and substances containing these as main components, such as powder from shellfish such as oysters, lime, etc. are used. The appropriate amount of the basic or neutral calcium-containing substance added is sufficient to bring the pH to around 7. This treatment removes acetic acid, propionic acid, and butyric acid in mash, vinegar, or its separated residue.

Dibasic acids etc. are neutralized.

The thus obtained treated product may be used as is. Also, the powder can be mixed with excipients if necessary,
It can be formulated into dosage forms commonly used for internal medicine such as tablets, capsules, syrups, granules, and suspensions.

Furthermore, it may be mixed with foods such as yogurt, candy, kutsky, and jelly, and may be mixed with other medicines, herbal medicines, food powders, extracts, and the like.

〔effect〕

The health food according to this invention contains mash obtained from acetic acid fermentation, vinegar or its separated residue, and calcium.
Due to these inherent effects and the effects of their coexistence, it has an excellent effect on lowering blood cholesterol (especially very low ratio bone lipoprotein and low ratio 1117 protein), increases body weight, liver triglyceride deposition, and body fat. Has a deposition inhibitory effect.

In addition, since volatile acids are neutralized, there is no strong sour taste or odor, making it convenient for consumption. Furthermore, since a basic or neutral calcium-containing substance is used for neutralization, there is no risk of large amounts of other metal ions such as sodium being included. Therefore, it is extremely excellent as a health food.

The health food of this invention is suitably ingested by an adult in an amount of 0.5 to 5 ml per day by the method described above.

〔Example〕

Next, this invention will be explained by examples, and its effects will be clarified by test examples.

Example 1 Black vinegar mash 60 K9 (solid/liquid mixture) and water 20
liter and stir vigorously with a homomixer (3440 rpm) to form a gel. Next, calcium hydroxide (1,451'f) was added to this while stirring with a homomixer.
) to adjust the pH to 7. Concentrate this under reduced pressure,
After drying, it is pulverized with a mixer to obtain a neutralized product 12.5 K9. At this time, separate the solid and liquid using a screw press,
It is efficient to dry the solid as it is under reduced pressure or by blowing air, concentrate the liquid under reduced pressure, and grind them together. As a result of analysis of the thus obtained neutralized product using a food analysis method, the content was 2.6% moisture, 18.3% protein, 44.1% lipid, 19.6% fiber, and 15.4% ash.

Example 2 Health food obtained in Example 1 150 parts lactose
90 parts The above ingredients are mixed and filled into hard gelatin capsules.

Example 3 Health food obtained in Example 1 80 parts Wheat flour 100 parts Shortening 15 parts Sugar
13 parts condensed milk
6 parts Sanwarm sugar
18 parts sodium bicarbonate
0.6 parts butter 1
0 parts water
6 parts Mix the above ingredients and bake to make biscuits.

Test Example 1 (Effect on obese mice) (Method) ICR male mice weighing 35 g were incubated at 24°C.
The animals were kept in an air-conditioned room with 65% humidity and artificial lighting (from 7 a.m. to 7 p.m.) for several days, and then intraperitoneally injected with 0.6 g of Gold thioglucose (Sigma Chemical Co.) per 7 days of body weight. did.

Animals that did not show a constant weight gain during the observation period of 2 weeks after injection were excluded, and the animals were divided into 4 groups and fed solid feed with the following composition.

1. Standard solid feed (Nippon Flare, CE-2)...
... Control group 2, solid feed with 3% black vinegar moromi neutralized product added to the above feed ... CT-Moromi neutralized product group 3, 3% black vinegar moromi neutralized product added to the above feed
Solid feed with % black vinegar moromi added...CT-Moromi group 4, solid feed with 3% black vinegar extract added to the above feed...
...Nippon Flare, CE-2 standard feed was administered to untreated control mice in the CT-Eski group.

Body weight increase and feed consumption were measured, and after being raised on the above diet for 6 weeks, the mice were decapitated and blood was collected, and internal organs such as the intestines, fat and liver lipids in the body excluding the head were quantified.

Serum glucose, total cholesterol, triglycerides, phospholipids, and free fatty acids were measured using enzymatic methods.

Serum high-density lipoprotein (HDL) cholesterol was obtained by precipitating and removing very low NE and low-density lipoproteins (VLDL, LDL) with phosphotungstic acid and magnesium chloride, and then removing cholesterol in the supernatant fraction by an enzymatic method. Quantitated. 1) For liver, add 1 g to chloroform/methanol (2:1°V/
Homogenize with V) and wash using Folch method 2).
1 and dried in a nitrogen stream. Weigh the dried edge lipids, redissolve them in a certain amount of chloroform/methanol, and
Cholesterol and triglyceride were measured using the 1750 using an enzymatic method. Phospholipids are Horflmeier
It was incinerated according to Fleet's method 3) and quantified by the molybdenum/leu method.

Body fat was extracted by homogenizing with chloroform/methanol, a portion of which was washed and dried using the Folch method 2), and the value was obtained by weighing.

(Conclusion) Black vinegar products were administered to obese mice induced by gold thioglucose for 5 weeks, and the effects on body weight, serum, liver lipids, and body fat were examined. In contrast, administration of black vinegar moromi had a weak effect, but administration of black vinegar moromi neutralized product increased body weight, increased blood cholesterol, especially ultra-low Nordic cholesterol, increased low-density lipoprotein cholesterol, liver neutral fat, and body fat. It was possible to suppress fat deposition.

(Reference) 1. Burnstein, M. et al.:
J, Lipid Res.

, 11: 583 (1970)・“□2, Fol
ch, J, et al.: J, Biol, Chem.
.

226.497 (1957) 3, Hoeflmyar, J., & Fr1ed, R.
:Med, Ernaehr.

7, 1 (1966) Test Example 2 (Acute Toxicity) (1) Product of Sample Example 1 (2) Appearance: Brown powder (3) Route of administration: Oral (4) Test animal: Mice, ddY-N male and female Age in weeks and starting weight: approximately 5 weeks old, male 24-26y, female 20-22y (5) Room temperature 22±2°C (6) Test period September 15th to September 22nd, 1980 (7) Preparation of test solution Method: Distilled water was added to sample 10y to make 100me, and the sample was homogenized for 15 minutes using a high-speed liquid homogenizer before use.

(8) Detection administration method, etc. (1) Administration method: Forced oral administration once using a gastric tube.

(2) Calculation method of LD5o value Probit method (3)
Administered concentration ratio of specimen: 1:1.2 (4) Number of animals per test group: 10 males and 10 females each (9) Test results (1) Symptoms of toxicity Immediately after administration, both males and females showed no signs of toxicity. No abnormalities were observed, and subsequent growth appeared to be almost normal.

(11) Autopsy findings No abnormalities were observed in major organs.

(121 Discussion In this test, no deaths were observed in either males or females even after administering up to 6,000 samples. This was because 9 doses were administered in 60 mL/cm as a detection signal, and the mice were forced once. This is considered the highest oral dose.

In addition, the detection results were muddy, making it impossible to administer with a stomach tube at higher concentrations. Therefore, the oral L of the specimen
The D5° value was set at 6,0001 nf/Kg or higher for both males and females.

Patent applicant Atsushi Iwamura - 2 patent attorneys (1 patent attorney) 1. Indication of the case Patent Application No. 220913 filed in 1982 2. Name of the invention Health food 3. Relationship with the case of the person making the amendment Patent applicant address 621-1 Oaza Takaida, Aihara City, Osaka Prefecture Name Materials
Jun -. Okay, two people.

4. Agent 5. Date of amendment order: February 26, 1985 (shipping date)
7 Contents of the amendment (1) In lines 9-13 of the third edition of the specification, the statement "Acetobactyl genus...Noyuetsuenbee" is corrected as follows.

"Acetobacter oquindans of the genus Acetobacter"
oxydans), Acetobactyl acetigenus (
Acetobacter ace-figenus),
Acetobactyl quinlinum (Aceto-bactc)
r xylinum), Acetobactyl acetosus (
Acetobacter ace [osus], Acetobacter acet
i), Acetobactyl quetinogianus (AccLo
bacLer kuetzingia+as), Acetobacter ascendens (Acetobacter ascendens)
asccndens), Acetobacter 5chuctzenb
achiii)j (2) On page 8, line 15 and page 9, line 3, the words rFolchJ are corrected to ``Holta (Folch) J.''

(3) rl, Burn on page 13, +o-+4i
5tein...Ernaehr, J is corrected as follows.

Claims (2)

[Claims] (1) Healthy products containing processed products that neutralize acidic volatile components and turn them into nonvolatile salts by treating mash, vinegar, or their separated residues obtained from acetic acid fermentation with basic or neutral calcium-containing substances. food. (2) The processed product is as it is, or is degreased, concentrated,
The health food according to claim 1, which is dried or powdered.