JPS6052761A - Measuring method for electrolyte - Google Patents

Abstract

PURPOSE:To enable electrolyte measurement which eliminates individual difference, improves substantially reliability and permits easy automation by measuring optically whether the specific material in blood cells is dispersed into a serum by hemolysis or not and discriminating the result of the measurement by the ion-selective electrode of the electrolyte in the serum in accordance with the result thereof. CONSTITUTION:The serum which is to be examined and is diluted by a buffer soln. 5 is dispensed in a reaction tube 3. Light from a white lamp 7 is projected through the tube 3 to the serum in a colorimetric measuring section 6. The light having 410nm wavelength and the light having 480nm wavelength of the transmitted light are successively made incident to a photodetector 9 by changing over a wavelength selecting filter 8. The outputs from the photodetector 9 by the respective incident light are supplied to a photometric circuit 10 which decides hemolysis when the difference between said outputs exceeds a predetermined reference value. The serum to be inspected with which the measurement is finished is then transferred to a flow cell 14 where the concns. of two kinds of ions are determined from the outputs of ion selective electrodes 12a, 12b by a measuring circuit 17. The concns. are printed out together with the information on the presence or absence of the hemolysis by a recorder and whether the measured value of the ion concn. is correct or not is discriminated.

Description

DETAILED DESCRIPTION OF THE INVENTION (Technical Field) The present invention relates to an electrolyte measuring method for measuring the concentration of specific ions in serum using an ion-selective electrode.

(Prior Art) In such an electrolyte measurement method, it is extremely important that the test serum is not hemolyzed in order to obtain highly reliable measurement results. For example, since a large amount of ions are contained in blood cells, measuring hemolyzed serum results in an abnormally high value, which may even cause misdiagnosis. Therefore, conventionally, the serum to be tested is visually inspected to determine whether hemolysis has occurred, and the results are displayed on, for example, a test slip to determine the measurement results using the ion-selective electrode. However, this method is insufficient in terms of reliability because the hemolysis determination varies depending on individual differences in the teeth being examined.In addition, as testing has become automated in recent years and the number of specimens processed has increased,
I can't handle it enough.

(Objective of the Invention) An object of the present invention is to provide an electrolyte measurement method that can solve the above-mentioned problems and sufficiently increase the reliability of measurement results using an ion-selective electrode, and can be easily automated. be.

(Summary of the Invention) When measuring electrolytes in serum using an ion-selective electrode, the present invention detects whether serum is hemolyzed or not by optical measurement such as colorimetry or flame photometry. The determination is made by detecting whether or not a specific substance contained in blood cells is dispersed, and based on the determination result, the measurement result of electrolytes in serum using an ion-selective electrode is determined.

(Example) Hereinafter, the present invention will be described in detail with reference to the drawings.

FIG. 1 is a diagram showing the configuration of an example of an apparatus for carrying out the electrolyte measuring method of the present invention. Test serum is in sample cup 1
A plurality of these samples are placed in a sampler that moves in the direction of arrow a through a predetermined sample dispensing position, and the first sample dispensing device 2 (only the nozzle is shown in the drawing) moves them through a predetermined transport path. The mixture is sequentially dispensed into reaction tubes 8 that are transferred in the direction of the arrow. Reaction tube 3 that received aliquot of test serum
In this step, the buffer solution 5 is dispensed using the buffer dispenser 4 to dilute the test serum. In this example, the test serum dispensed into the reaction tube 8 and diluted with the buffer solution 5 is first colorimetrically measured through the reaction tube 8 in the colorimetric measuring section 6 to determine the presence or absence of hemolysis. .

Here, in the spectral absorption spectrum of hemolyzed serum, since hemolyzed serum contains a large amount of ions, as shown in Figure 2, the absorbance peaks at around a wavelength of 410 nm, and from there it reaches a wavelength of 480 nm. The absorbance decreases rapidly up to around 620 nm, and then decreases gently as a whole, although there are some ups and downs up to around 620 nm. That is,
Hemolysed serum has an absorbance at a wavelength of 410 nm and an absorbance at a wavelength of 4
The difference from the absorbance at 80 nm is significantly large.

Therefore, in this example, the light from the light source (white lamp) 7 is projected onto the reaction tube a containing the test serum, and among the transmitted light, the light with a wavelength of 410 nm and the light with a wavelength of 480 nm are separated by the double arrows. By switching the wavelength selection filter 8 that is movable in the direction shown, the light is sequentially incident on the light receiving element 9, and the output of the light receiving element 9 due to each incident light is supplied to the photometry circuit 10, and the difference (g & luminous intensity) between the outputs is calculated. When the value (corresponding to the difference) exceeds a predetermined reference value, hemolysis is determined. In this example, information indicating the presence or absence of hemolysis from the photometric circuit 10 is printed out by a recording device (not shown) together with the electrolyte value measured by an ion-selective electrode, which will be described later, in the serum to be tested.

The test serum that has undergone colorimetric measurement in the colorimetric measurement section 6 is
In this example, at a predetermined dispensing position, the liquid is dispensed into an inlet 15 communicating with a second flow cell 14, and transferred to the flow cell 14 by the operation of a rolling pump 16.
In the blow cell 14, ion selective electrodes 12a, 12
Two kinds of predetermined ion concentrations are determined in the measuring circuit 17 from the output of b. The ion concentration determined by the measurement circuit 17 is printed out by a recording device (not shown) together with information indicating the presence or absence of hemolysis in the test serum by the photometry circuit 1o described above, and based on the information indicating the presence or absence of hemolysis, Determine whether the measured value of ion concentration is correct or not.

In the flow cell 14, the test serum whose electrolytes have been measured by the ion selection tta is transferred to the rolling pump 16.
The liquid is discharged into the waste liquid tank 18 by the operation of .

According to this embodiment, the predetermined ion concentrations of a plurality of test sera can be sequentially and automatically measured while determining the presence or absence of hemolysis in the test sera. Since the information representing hemolysis and the electrolyte measurement value for the same serum are printed out at the same time, it is possible to easily determine whether the electrolyte measurement value is good or bad, and the reliability of the measurement value can be increased.

Note that the present invention is not limited to the above-mentioned example, and can be modified or modified in many ways.

For example, hemolysis can be determined not only by detecting ions but also by detecting other specific substances contained in blood cells. In addition, hemolysis can be detected not only in terms of its presence or absence, but also quantitatively, and in addition to the above-mentioned colorimetric measurement, the flame side-light method can also be applied to detect hemolysis. Furthermore, in the above-described example, when detecting hemolysis, the test serum was directly photometered through the reaction tube 8, but it can also be configured to be guided to a flow cell and photometered. In addition, the electrolyte allows the ion-selective electrode and the reference electrode to be raised and lowered,
It can also be configured to be directly immersed in the test serum in the reaction tube 8 for measurement. Fourth, the order of detecting hemolysis and measuring electrolytes using an ion-selective electrode may be reversed;
Furthermore, in the case where hemolysis is detected first as in the above-described embodiment, it is also possible to omit the next measurement of electro-FIV quality using an ion-selective electrode for the test serum determined to be hemolyzed. In the above example, hemolysis and electrolytes were measured while the test serum was automatically transferred, but hemolysis detection and electrolyte measurement may be completely separated. .

(Effects of the Invention) As described above, according to the present invention, it is possible to optically measure whether or not a test serum is hemolyzed, and to select an electrolyte ion in the test serum based on the measurement result. Since the measurement result using the electrode is determined, the reliability of the measurement result using the ion-selective electrode can be sufficiently increased. Furthermore, since hemolysis is detected by optically measuring a specific substance contained in blood cells, it can be easily automated.

[Brief explanation of the drawing]

FIG. 1 is a diagram showing the configuration of an example of an apparatus for carrying out the electrolyte measurement method of the present invention, and FIG. 2 is a diagram showing an example of the spectral absorption spectrum of hemolyzed serum. 1... Sample cup 2... First sample dispensing device 3... Reaction tube 4... Buffer solution dispensing device 5... Buffer solution 6... Colorimetric measurement section? . . . Light source 8 . . Wavelength selection filter 9 . . . Photodetector 1o .

Claims (1)

[Claims] L When measuring the electrolyte in the collected serum using an ion-selective electrode, it is optically measured whether a specific substance contained in blood cells is dispersed in the serum due to hemolysis, and the measurement result is 1. A method for measuring electrolytes, comprising determining a measurement result of an electrolyte in the serum using an ion-selective electrode based on the method.