JPS60246304A - Controller for pine withering blight - Google Patents

Abstract

PURPOSE:An agent for remedying or preventing pine withering blight, comprising 2-(thiocyanomethylthio)-benzothiazole known as a mildewproofing agent, agricultural chemical, etc. as an active ingredient. CONSTITUTION:An agent comprising 2-(thiocyanomethylthio)-benzothiazole shown by the formula as an active ingredient, capable of destroying a pine withering mold by sterilizing or controlling it. Although this compound with a low concentration can destroy the pine withering mold, it will not show phytotoxicity even if it is injected into pine trees and oak trees in a fairly high concentration. Since in this method trees are individually treated, preferably trees that are grown more a certain degree than small young trees, especially trees of older than 10 years are preferentially treated.

Description

[Detailed description of the invention] (b) Industrial application fields The present invention relates to a control agent for pine blight.

(Talk) Conventional technology In Japan, pine damping-off is widespread. Pine damping-off is a type of epidemic wilt that affects all specimen species.
It is generally called pine blight because the damage caused by so-called powder is noticeable. This disease is said to be caused by pine trees being attacked by the pine tree nematode. Injection into tree trunks has been carried out, but pine blight has not been completely controlled.
It has become a big problem.

One of the inventors of the present invention previously discovered Hablo Nubolev (Ha.
ploSpOrella) genus, phomos
is) horses, Phylyctoana spp.
Some molds such as those of the genus MaOrOphOma and Diplo-di, a, have the ability to wither pine trees.
Since molds have been found in pines suffering from pine blight, he proposed that these molds are the cause of pine blight (Hereinafter, among the molds of these genera, A substance that has the ability to destroy specimens is called pine blight bacteria.
.

On the other hand, the pine blight nematode feeds on the ``mold'' that lives inside living pine trees, and is particularly fond of eating pine blight fungus.

Therefore, regardless of whether pine blight is caused by pine blight or pine blight, controlling pine blight will lead to control of pine blight.

←) Objective The objective of the present invention is to provide a drug that can control pine blight disease by sterilizing or bacteriostasis of pine blight 1.

As a result of intensive research on the prevention of pine blight, the present inventors found that
2-(thiocyanomethylthio)~benzothiazole is
Although it is possible to kill the pine blight bacteria even at a low concentration, the inventors have discovered that even when injected at a considerably high concentration of K in the tree of the phyllophore, no chemical damage occurs, and the present invention has been achieved.

(=) Structure and Effects That is, the present invention provides a pine blight control agent containing 2-(thiocyanomethylthio)-benzothiazo-1 L/l as an active ingredient.

2-(Thiocyanomethylthio)-benzothiazole used in the present invention is a compound having the chemical structure shown in Formula 1.

This compound is widely used as an industrial fungicide for preserving wood, leather, sealants, adhesives, etc. As a pesticide, an emulsion containing 30% of this compound i is registered under the name benzothiazole emulsion and is used to disinfect seed rice to prevent sesame leaf blight in rice, but it causes chemical damage when used for sprouting rice. Therefore, the instruction is to make sure to thoroughly process the dry rice.

2-(Thiocyanomethylthio)-benzothiazo-/%/
Forest, Buckma
N Laboratories, Inc.) manufactures and sells it under the trade name Busanso 80, but the content of 2-(thiocyanomethylthione-benzotheazole) in this product is approximately 80% by weight, and cutting is not required. This active ingredient is diluted with hydrogen aromatization to give a total content of 2-(thiocyanomethylthio)-benzothiazo-y of about 30% by weight.
A product called (BuSan 30) is also on sale.

A drug containing the 2-(thiocyanomethylthio)-benzothiazole of the present invention is injected into the interior of a tree where pine blight is to be treated or prevented. Injection ratio a of 2-(thiocyanomethylthio)-benzothiazo-ρ, size of #■
(unit: m3), 1 to 5009/m”, especially 5 to
It is preferable to set it to 1005'/yn'. The size V of the tree is calculated from the circumference L (unit m) of the trunk at a height of 1.5 m from the ground and the height H (unit m) of the tree.
This is the value calculated by formula (■).

The value of ■ in 4 corresponds to the volume of a cylinder having a circumference equal to the circumference of the tree's trunk at a height of 1.51n from the ground and a height equal to the tree height ■.

Byusan 80 is a viscous liquid that solidifies in winter. Therefore, Vyusa 780 is usually diluted to an appropriate concentration before use. Piusan 30 can be injected into the tree as it is, but it may also be diluted before use. The medium used for dilution is preferably water and/or an organic solvent. and 2-(thiocyanomethylthio)-benzothiazo-/L
' is dispersed (emulsified) or dissolved in a diluting medium. Dispersion (
It is customary to use an emulsifier during the FL conversion.

The medium used for dilution should be one that does not cause phytotoxicity to the trees being injected. Examples of preferably used solvents include L-aromatic hydrogen, cyclohexanone, ethylene glycol monobutyl ether, dimethyl sulfoxide, and dimethyl formamide. These solvents may be used alone or in combination of two or more. By placing the entire emulsifier in these solvents, 2-(thiocyanomethylthio)-benzothiazo=P can be contained in an amount exceeding the saturation solubility. In addition, 2-(thiocyanomethylthio)-benzothiazole is dispersed (emulsified).
Examples of the medium used for this purpose include water, a water-methyl alcohol mixture, a water/ethyl alcohol/L' mixture, a water/acetone mixture, and a water/ethyl alcohol/acetone mixture. It is also possible to mix the above solution 11 in a dispersion (emulsification) medium and use it. Methyl alcohol, ethyl alcohol, acetone, etc., when used as 'kR form, 2-
(Thiocyanomethylthio]-benzothiazole is absorbed into the ladle at a significantly higher rate. Ethyl alcohol/I
When used by mixing with water-containing acetone and acetone, the water content in the mixture is preferably 40% by weight or more, particularly 50% by weight or more. Preferably, the emulsifier is selected from nonionic or anionic surfactants.

Examples of these surfactants include alkyl μ ether type, alkyl allyl ether type, alkyl ester type,
Nonionic surfactants such as sorbitan monoalkyl ester μ type, polyoxyethylene alkyl amine, oxyethylene-oxypropylene block copolymer reduced, higher alcohol ρ sulfate ester salt, polyethylene glycol ether/L/sulfur ester Salt, olefin sulfate ester/l
/ salts, amide-bonded sulfuric ester salts, alkyl sulfonates, alkylaryl sulfonates, and other anionic surfactants.

In the method of the present invention, 2-(thiocyanomethylthio)-
Solutions, emulsions, and dispersions of benzothiazole (hereinafter referred to as
These are collectively called drugs. ) into the tree, make a hole tS at an appropriate depth, e.g.
Insert the drug injection nozzle/L/ into the hole in the container containing the drug and inject the entire drug in the container using gravity formation pressure, or mix it with the solvent or dispersion medium used for the drug. It is possible to adopt a method such as adding a polymeric substance to a drug, making it into a paste or stick form, and pushing it into a hole of an appropriate size in a tree trunk or cane.

Examples of the polymeric substances used here include styrene-butadiene copolymer (when the solvent is aromatic hydrocarbon), crushed boiled rice or starch paste (when the dispersion medium is mainly water-metal), etc. be done.

When injecting chemicals into the trunk or cane of a tree, there are no particular restrictions on the location of the injection port as long as the trunk or cane does not break after making the hole, but if you inject the medicine from the trunk of the tree as close to the ground as possible, the medicine will be effective. This is preferable because it quickly spreads throughout the tree.

Further, the direction of the holes does not necessarily have to be perpendicular to the surface of the tree trunk or cane, but may be perpendicular to the surface of the tree trunk or cane. Perforation and drug injection can be carried out regardless of the season, but it is preferable to do it in winter because there is less spouting out of the bud.

In the method of the present invention, the drug can also be injected into the tree by absorbing it through the roots of the tree. In this case, it is advisable to dig out the roots in the soil, thoroughly wash off any soil that has adhered to them, and then apply the tip of the root to the chemical contained in the container.

Species are susceptible to so-called pine blight. Some of these tree species are susceptible to damage if they are artificially moved to warmer areas, even if their native habitat is cold and they do not cause much damage. Therefore, the method of the present invention is applicable to all phase inversion classes in general. Typical tree species include fir, hemlock, spruce, turnip pine, red pine, black pine, ryukyu pine, Japanese pine, rainbow pine, Sakhalin pine, and cedar.

Since the method of the present invention is carried out on individual trees, it is more valuable to preferentially carry out the process on mature trees that have grown to a size larger than small seedlings. It is preferable to give priority to trees that are 5 years old or older, especially trees that are 10 years old or older.

2-(Thiocyanomethylthio]-benzothiazo- can kill the pine blight fungus at extremely low concentrations, and does not cause any chemical damage to specimens even at extremely high concentrations. In an experiment to determine the minimum inhibitory concentration of the pathogen, a concentration of 0.5 ppm of 2-(thiocyanomethylthio)-benzodeacyl was able to inhibit the growth of the pine blight fungus. 〃vinegar)
In a toxicity test for calli, no damage was observed to calli even when an aqueous dispersion of 2-(thiocyanomethylthio)-benzodeazole at a concentration of 500 ppm was applied to calli. In other words, 2-(thiocyanomethylthio)-benzothiazole has a minimum inhibitory concentration of 1% against pinecone bacterium.
It was found that it is a substance that does not cause any damage to specimens even at a concentration of 1,000 times, has excellent medicinal efficacy, and is less likely to cause drug damage.

To detect whether pine blight has already invaded a tree, cut the trunk or trunk of the target tree into large pieces (approximately 1 m square in thickness, 5 in length (inside and outside of ffi). After washing for a short time with 1,000 times higher ice water and placing it in a flat plate for 2 to 3 hours, the sample was cut into pieces with a thickness of about 11 qn using pruning shears, and the begton and yeast extracts were placed on Kanakura Muzapek medium. It is preferable to culture at 25 to 30° C. in several places.By this method, bacteria distributed inside one material can also be detected.

The method of the present invention can be applied to specimens that have already been invaded by Pinus blight to exterminate the Pinus blight and restore the tree to a healthy state. It can also be applied to specimens to prevent the invasion of pine blight.

Hereinafter, the present invention will be explained in full detail with reference to Examples.

(e) Examples Example 1 Whole specimens were collected from a group of Japanese black pines at a tree height of approximately 5 ms and a height of 1.5 m from the ground, with a trunk circumference of approximately 30 mm, and examined for the presence of pine blight bacteria.

Based on the test results, 5 trees in which pine blight was detected from this group
A book was selected, and 30'i of Byusan was used as a drug, which was injected into the trunk near the ground.

The injection method is to install a nozzle Ivt at the lower end of a small pressure vessel containing Byusan 30, insert the tip of the nozzle into a hole in the tree trunk, and inject Byusan 30 using air pressure, at four locations on one tree trunk. Using a total of four small pressure vessels containing drugs, one in each hole, the total short sound 2-(thiocyanomethylthio)-benzothiazo-μ of the drugs in the four containers is the size of the tree V. 200 r/m'.

After observing the progress for 12 months after injecting the chemical, no chemical damage was observed on the tree. Twelve months after the chemical injection, a portion of the tree was cut out and examined for the presence of pine blight, and it was found that pine blight was extinct in all trees.

Example 2 The following drug formulation was prepared (Preparation A).

a, Byusan 80 12.5 parts by weight, Emulmin 240 (nonionic surfactant, manufactured by Sanbe Kasei Kogyo Co., Ltd.) 1.5 parts by weight C, Pronik L-64 (nonionic surfactant)・Asahi Denka Kogyo Co., Ltd. product) 1.5 quintillion parts d, Ionet T-eoc (nonionic surfactant, Sanbe Kasei Kogyo Co., Ltd. product) 1.5 parts by weight e, Ionet DO-aoo ( 11' Ionic surfactant/Sanbe Kasei Kogyo Co., Ltd. product) 1.5 parts by weight f, Nonipor/l/110 (Nonionic surfactant/Sanbe Kasei Kogyo Co., Ltd. product) 1.5 Weight part g, ethyl alcohol
10.0 quintillion parts by weight, 70.0 parts by weight of water. First, mix well a-fi. G again! : Mix thoroughly with h, then add g, ! while stirring the mixture of a to f. :H mixture was added dropwise and emulsified to form an emulsion. This emulsion contains 2-(thiocyanomethylthio)-benzothiazo-tvfjc1o%J, 96.

Whole canes were harvested from a group of Japanese red pines with a trunk circumference of approximately 30 c7+ at a height of approximately 5 m and a height of 1.5 m from the ground, and were t-tested for the presence of pine blight.

Based on the test results, 5 trees in which pine blight was detected from this group
A book was selected and used as a medicine (Preparation AJ't), which was injected into the trunk near the whole ground.

The injection method was to install a nozzle/Q in the lower part of a small pressure vessel containing 1 drug AJt, insert the tip of the nozzle into a hole in the tree trunk, and inject using air pressure.1 By drilling four holes in the trunk of a book and using a total of four small pressure vessels filled with chemicals, one in each hole, the total amount of the chemicals in the four vessels was measured. The size of the tree was set at 40,097 m.

After observing the progress for 12 months after injecting the chemical, no chemical damage was observed on the tree. Twelve months after the chemical injection, a section of the tree was cut out and examined for the presence of pine blight, and it was found that pine blight was extinct in all trees.

Example 3 Canes were collected from a group of Japanese black pines at a tree height of approximately 5 m and a trunk circumference of approximately 30 mm at a height of 1.5 m from the ground and examined for the presence of Pinus blight.

Based on the test results, 5 trees in which pine blight was detected from this group
A tree was selected and rU17'1IIAJ was used as a drug and injected into the trunk near the ground.

Injection method: One hole is drilled in each trunk, and the tip of the nozzle installed at the bottom of the small pressure vessel containing [Preparation A] is inserted into the hole, and [Preparation A] is injected using air pressure. Nine. The amount of the drug in the shed pressure vessel was 5K such that 2-(thiocyanomethylthio)-benzothiazo-~ was 1 ot/m' with respect to the tree size V.

After observing the progress for 12 months after injecting the drug, no chemical damage was observed in the ladle. Twelve months after the chemical injection, some branches were cut and examined for the presence of pine blight, and it was found that pine blight was extinct on all trees.

Example 4 The following formulation was prepared as a drug (Preparation B).

a, Byusan 80 3'/, 5 parts by weight, Vegan-lvR (aromatic hydrogenated solvent, manufactured by Mobil Oil Co., Ltd.) 41.0 parts by weight C, Emulmin 24o (nonionic surfactant [IJ-3) Part Kasei Kogyo Co., Ltd. product) 2.6 parts by weight d, stearic acid 2.6 parts by weight e, jetanolamine o, 9111 parts f, water 15
．． 4 parts by weight First, a solution is made by mixing c-f while heating.Next, while stirring this solution, a solution of a dissolved in b is added dropwise to emulsify it, and when it is left to cool, a paste is formed. A similar preparation was obtained. This preparation is 2-(thiocyanomethylthio)
Contains 30% by weight of -benzothiazo-.

Canes were collected from a group of Japanese black pines at a tree height of approximately 10 fi and 1.5 m above the ground, with a trunk circumference of approximately 60 mm, and examined for the presence of pine blight.

Based on the test results, trees where pine blight was detected from this group2
I selected a book and injected it with "Preparation B."

To inject "Preparation B", drill a hole with a Kjl diameter of 1 m and a depth of 6.5σ at a height of about 30 meters from the ground in each tree trunk, and pour "Preparation B" 52 into this hole. The method was adopted. At this time, the ratio of 2-(thiocyanomethylthio)-benzothiazole to the tree size v is approximately 5
It was -.

The tree was followed for 12 months after the drug was injected, and no signs of drug damage were observed. Twelve months after the chemical injection, a portion of the tree was cut out and examined for the presence of pine blight, and it was found that pine blight was extinct on both trees.

Example 5 Two larch trees with a tree height of approximately 10 m and a trunk circumference of approximately 30 m (!*) at a height of 1.5 m from the ground were selected, and each trunk was inoculated with Pinus blight.

The method of inoculating the fungus is to cut the bark at a height of 30cm from the ground in a double-shaped pattern up to the cambium layer, and the length is 53 cm.
Cut it open and attach pine bacterium to a length of about 1cm.
A method was adopted in which 2 to 3 In wheat culms were put in and the bark was closed back to its original state. To attach the fungus to the wheat culm, a length of about 1 (!I
A few barley culms from No. 11 were put in, sterilized, and inoculated with pine blight.
After culturing at ~30°C, the wheat culm may be removed.

Three months after inoculating the pine blight fungus, 1.
When we collected cylindrical pieces of wood with a diameter of 1cm and a depth of 1clR at a height of 5 m and examined them for the presence of pine blight bacteria, pine blight bacteria was detected on both of the inoculated trees. "Preparation A" was injected into the tree.

The injection method is to drill one hole in the trunk near the ground and use "Preparation A"
The tip of the nozzle at the bottom of the small pressure vessel containing the liquid was inserted into this hole, and ``Preparation A'' was injected using air pressure. The amount of "Preparation A" used was such that 2-(thiocyanomethylthio)-benzothiazole was 1017 m''K for the tree size V.

After observing the progress for 12 months after injecting the chemical, no chemical damage was observed on the tree. Twelve months after the chemical injection, a portion of the cane was cut off and examined for the presence of pine blight, and it was found that pine blight was extinct on both trees.

Agent: Patent Attorney Shintabe Nogawa

Claims (1)

[Claims] 1.2-(thiocyanomethi pthio]-benzothiazo-/
A pine blight control agent comprising 1/l of active ingredients.