JPS60220862A - Instrument for chemical analysis - Google Patents

Abstract

PURPOSE:To enable always exact analysis by spotting the specified quantity of a sample liquid to sheet-like analyzing elements, loading such elements to carriers, arraying the carriers in two rows, feeding successively the carriers and providing the 1st optical measuring means and the 2nd measuring means for correcting the measured value thereof. CONSTITUTION:The sheet-like analyzing element 1 formed by providing successively a reagent supporting layer, reflecting layer and developing layer on a transparent base and holding the base to a slide frame is fed to a loading base 31 where a specified quantity of the liquid sample 9 is spotted onto said element. The element 1 is then loaded to the carrier 23 through the inlet 23a of the carrier 23 by a pushing lever 35. The carriers arrayed in two rows on a constant temp. plate 21 having a housing part 22 made into a rectangular shape are transferred successively in the arrow direction shown by a dotted line by pushing levers 25-27 and the color developing density of the reagent holding layer is measured in a measuring part 39 for basic value at a measuring point 38. One or more of the developing area and developing diameter of the element 1 or the reaction area and reaction diameter of the reagent holding layer are measured in an auxiliary measuring part 43 after the element is dropped into a chute 41. The coloring developing density of the sample liquid failing to indicate the normal developing and reaction area, etc. is corrected and the exact analysis is attained.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a chemical analyzer for quantifying a test component present in a liquid sample using a sheet-like analytical element, and more specifically relates to a chemical analyzer for quantifying a test component present in a liquid sample using a sheet-like analytical element. Some blood.

The present invention relates to a chemical analyzer used in a clinical chemistry testing system that performs quantitative analysis of specific components present in urine and the like.

[Prior art]

Sheet-like analytical elements comprising at least one reagent layer provided on a transparent support are disclosed in Japanese Patent Publications Nos. 49-5888, 187192-1982, 40191-1981, 8488-52, No. 58-181089, No. 54-
No. 101898, No. 57-66859, No. 55-16
No. 4856, No. 57-148250, No. 56-245
No. 76, Utility Model Application No. 57-42951, U.S. Patent No. 829
No. 8789, No. 8895082, Journal of the American Society of Clinical Chemistry
885-1850Jj (1978), vol. 27, 12
87-1290 (1981), etc.

FIG. 1 shows an example of this sheet-like analysis element. In the sheet-like analysis element 1, a measurement element 4 formed into a dry multilayer film is held between two upper and lower plastic slide frames 2 and 8. The upper slide frame 2 is located on the sample spotting surface side, and a liquid sample such as blood, etc. is placed in the center of the upper slide frame 2.
Serum, plasma.

A hole 2a is formed into which body fluids such as urine are deposited.

The lower slide frame 8 is located on the analytical photometry surface side, and has a hole 8a formed in its center for passing light.

These sliding frames 2.8 accommodate the measuring element 4 and are then welded together at their peripheral edges to be integrated.

The measurement element 4 has a transparent support 5. Reagent holding layer 6
9 Reflective layer 7. It is composed of a development layer 8. The transparent support 5 may have a thickness of 1851z after being undercoated, for example.
The hole 8a of the lower slide frame 8 is closed by the transparent support 5, and the reagent holding layer 6 is attached to the soil of the transparent support 5. A reagent is used that reacts with the target component contained in the body fluid and develops a color at a concentration that corresponds to the amount of the component. For example, when measuring glucose concentration in blood, glucose oxidase (TOYOBO GOD III) 501T9
, Bell Oxytase (POD ■ manufactured by Toyobo) 20■, 1,
A solution consisting of 185 cm of 7-hydroxynaphthalene, 180 cm of 4-aminoantipyrine, 20 q of polyoxyethylene nonylphenyl ether (Nonion H8210 manufactured by NOF Corporation), 8.5 F gelatin, and 100 N water was used,
It is applied onto the transparent support 5 so that the dry film thickness is 15 μm. The reflective layer 7 prevents light that has entered the reagent holding layer 6 through the hole 8a of the lower slide frame 8 from reaching the developing layer 8 and thereby preventing the color of the body fluid in the developing layer 8 from being photometered. , for example, titanium oxide 6.8f, gelatin 2. 'l
f, polyoxyethylene nonylphenyl ether 100
A uniform dispersion liquid consisting of 50 μm of water is used and applied onto the reagent holding layer 6 so that the dry film thickness is 8 μm.

The developing layer 8 is made of cotton, for example, so that when a small amount of body fluid, for example 10 μl, is dropped from the hole 2a of the upper slide frame 2, it is spread in spots with uniform density and the same size. Broad (100 sou manufactured by Toyobo) 0
．． The developed layer 8 is treated with an aqueous solution of polyoxyethylene nonyl phenyl ether.
Crimp and laminate on top.

When performing quantitative analysis using the sheet-like analytical element 1,
As shown in FIG. 2, a certain amount of a liquid sample 9, for example, a fresh heparinized blood sample, is dropped from the hole 2a of the upper slide frame 2.
Place it on the spreading layer 8. The fresh surface 9 spotted on this spread layer 8 spreads laterally and is usually approximately circular or elliptical (this is called a spread layer).The fresh surface 9 spread on this spread layer 8 is passing through the reflective layer 7), reaching the reagent holding layer 6 1,
Here, it undergoes a color reaction with the reagent. In this reagent holding layer 6, the developing layer develops a color with the same magnitude as tx.

After spotting the liquid sample, the sheet-like analytical element 1 is incubated 7 to allow a sufficient color reaction to occur. After this incubation, the reagent holding layer 6 is irradiated with illumination light from the hole 8a of the lower slide frame 8, and the light of a specific wavelength contained in the reflected light is measured to determine the color concentration, and the color density is determined in advance. By referring to a standard curve showing the color density vs. component amount, colorimetric analysis of a specific component present in blood, such as glucose component, can be performed.

As a chemical analyzer that performs quantitative analysis using the sheet-like analytical element 1, for example, Japanese Patent Application Laid-Open No. 58-21566,
The chemical analyzer disclosed in JP-A No. 58-76095 and U8P4296069 is known. However, since this chemical analyzer only measures the reaction result of the reagent holding layer 6, that is, the coloring concentration or coloring rate, the liquid There is a problem in that it is not possible to obtain satisfactory results for samples that deviate from the normal range. i.e. plasma,
Blood samples such as serum and whole blood have differences in the amount of formed components, protein concentration, viscosity, etc. depending on the sample, and their fluidity also changes with time and additives. This is because it is affected by the development of the situation. In a normal blood sample, the size of the spread layer is l”t
However, if it deviates from the normal range, spreading will not be sufficient, so the coloring circle in the reagent holding layer 6 will become extremely small, and the coloring density and coloring speed will be extremely reduced.

[Purpose of the invention]

The present invention has been improved so that good analysis results can be obtained even for liquid samples whose properties are outside the normal range.
The purpose of the present invention is to provide a chemical analysis device that provides a chemical analysis system with a high level of performance.

[Structure of the invention]

In the present invention, a carrier is circulated in a storage section provided in a constant temperature plate, a sheet-like analytical element on which a liquid sample is spotted is loaded into the carrier, and both are intermittently transferred together, and during this intermittent transfer, The sheet-like analytical element is incubated at a constant temperature for a certain period of time, and after this incubation, the first
The reaction result is optically measured by the second measuring means, and the area, diameter or developed color density of the developed layer, and any one of the area or diameter of the coloring circle are measured with the auxiliary value and 17. Then, the obtained auxiliary value is used to correct the reaction results. The measurement of the auxiliary value by the second measurement means may be performed after the liquid sample is spread on the spreading layer, and may be performed during incubation or before or after incubation.

To measure the diameter and area of the developing layer or coloring circle, a light source that irradiates the developing layer and a group of light receiving elements arranged in a line or COD image sensor-1 or one light receiving element are used, When measuring developed color density, a light receiver is used that can measure an area smaller than the smallest possible developed layer.

〔Example〕

Embodiments of the present invention will be described in detail below with reference to the drawings. .

FIG. 8A shows the mechanical configuration of the present invention, and FIG.
Figure B shows the cross section. A constant temperature plate 21. A flange 19 is attached. This constant temperature plate 21 has a storage part 22 formed in the center thereof, and a plurality of carriers 28 are arranged in a length of eight in the horizontal direction.
Fifteen carriers 23 are stored in order to intermittently transport 8. It goes without saying that a partition wall may be provided in the center to form a rectangular circulation path. , push levers 25 to 28 are provided at each corner of the storage section 22,
1 for transferring one piece of carrier 28 at the corner, insertion port 8
A loading table 81 is connected to the loading table 81, and the sheet-like analysis element 1 that has descended through the chute 82 enters the recess 81a of the loading table 81. A certain amount of the liquid sample 9 dropped from the micropipette 88 is spotted on the spread layer 8 of the sheet-like analysis element 1 that has entered the recess 81a.

After this spotting, the motor 84 rotates and the push lever 85
is moved back and forth, and the sheet-like analysis element 1 is put into the storage section 22 through the insertion port 80. The sheet-like analysis element l that has entered the storage section 22 is transported to the carrier 2 facing the insertion opening 80.
8 through the insertion opening 28a.

The carrier 23 into which the sheet-like analysis element 1 has been inserted is intermittently transferred one frame at a time in the direction shown by the dotted line, as the pushing levers 25 to 28 are sequentially operated by the rotation of the motor 86. During this intermittent transfer, the sheet-like analysis element 1 is transferred to the constant temperature plate 2.
1, the sample is incubated at, for example, 87° C. for a certain period of time.

The incubated sheet-like analysis element 1 has a measuring point 88
reach. A basic value measuring section 89 is provided below this measurement point 88, and the color density developed in the reagent holding layer 6 is measured as a basic value. It falls through a discharge port 40 (see Fig. 4) formed at the bottom of the position where the push lever 27 enters and enters the chute 41.The chute 41 is provided with two stop claws 42, and the chute The sheet-like analysis element 1 sliding on the sheet-like analysis element 1 is stopped.An auxiliary value side leg 48 is provided to measure the stopped sheet-like analysis element 1, and the developed color density is measured as an auxiliary value.

After this measurement, the motor 44 rotates to release the stop pawl 42.

An unused sheet-like analysis element 1 is stored in the storage tube 45 . When the cam 47 is rotated by the three motors 46, the ejection lever 49 reciprocates due to the engagement between the pin 48 of the cam 47 and the groove 49a, and pushes out the sheet-like analysis element 1 located at the lowest part of the storage tube 45. The sheet-like analysis element 1 pushed out from the storage tube 45 flows down the chute 82 and enters the recess 81 a of the loading table 81 .

The storage cylinder 45 is fixed to a transparent cover 50 via a chute 82. This cover 50 is fixed on the constant temperature plate 21. .

FIG. 4 shows a drive device for the push-in lever.

A cam 58 having a shape with a part of its periphery cut out is provided on the rotating shaft 52 of the motor 36. Four followers 54 to 57 are swingably provided in contact with the cam 5B. Since the mechanism for connecting each follower and the pushing lever is the same, only the follower 54 will be explained. A roller 60 is provided on one end of the cam 58, which is rotatably supported by a shaft 59.

A connecting rod 61 is connected to the other end of the follower 54, and this lever 62 is pivotally supported by a shaft 68, and a roller 64 is attached to the tip.
is installed. This roller 64Fi fits into a U-shaped rail 65 provided on the pushing lever 25.

When the cam 58 rotates and its notch comes to the position of the follower 54, the follower 54 rotates counterclockwise, causing the lever 62 to rotate counterclockwise via the connecting rod 61. When this lever 62 is rotated counterclockwise, the push lever 25 moves rightward to transfer the carrier 28 by one frame. When the cam 58 rotates one more time and the notch moves away from the follower 64, the push lever 25 moves to the left and retreats from the storage section 22.

In this way, when the cam 53 rotates, the push lever 25
.about.28 are operated in sequence, and one rotation advances all the carriers 28 in the storage section 22 by one frame.

A pushing lever 85 for loading the sheet-like analysis element 1 into the carrier 28 is rotated back and forth by a motor 84 to move back and forth. This cam 67 is synchronized with the cam 58 and moves forward while the push lever 27 is stopped. Further, instead of using this cam mechanism to drive the push levers 25 to 28, a motor or a solenoid may be provided to each push lever 25 to 28, and these may be driven independently.

FIG. 5 shows the configuration of the basic value measuring section.

A hole is formed in the constant temperature plate 21 at the measurement position 88,
A dark box 72 is provided below this hole.

Inside this dark box 72, a plurality of illumination light sources 78. Lens 749 held in lens barrel 75 Color filter 76° Photodetector 77
is provided.

The sheet-like analysis element 1 at the measurement position is illuminated from the transparent support 5 side by illumination light emitted from the illumination light source 78. The light reflected by the reagent holding layer 6 of the sheet-like analytical element 1 passes through the transparent support 5, further passes through the lens 749 and the color filter 76, and enters the photodetector 77, where only light in a predetermined wavelength range is photodetected. It is converted and the basic value is measured as before.

When the pushing lever 28 moves forward after measuring the basic value, the carrier 28 moves to the discharge port 40, and only the sheet-like analysis element 1 falls from the discharge port 40 and enters the shoe 41.
When this pushing lever 28 is moving forward, a white point 80 provided on its lower surface is measured and used as a reference value for the reflectance zero or a specific percentage.

FIG. 6 shows the configuration of the auxiliary value measuring section.

The sheet-like analysis element 1 that has fallen from the discharge port 40 is guided onto the chute 41 by the guide plate 82, and is stopped by the stop claw 42 while sliding on the chute 41. The auxiliary value measuring section 48 includes a mounting base 88, and an illumination tube 84 and a light receiving tube 85 fixed to the mounting base 88. A light source 86 and a lens 87 are housed in the illumination tube 84, and a lens 889 and a color filter 8 are housed in the light receiving tube 85.
A 9° photodetector 90 is housed, and the developed color density is measured as an auxiliary value. The color filter 89 can be omitted if a light source 86 that is suitable for detecting the color of the liquid sample and has wavelength selection characteristics, such as an LED+light emitting diode, is used.

After measuring this auxiliary value, the stop claw 42 is moved to the dotted line position by the motor or the solenoid 44, and the sheet-like analytical element 1
Allows for sliding.

FIG. 7 shows the electrical configuration of the present invention.

The output signals of the basic value measuring section 89 and the auxiliary value measuring section 48 are
It is selectively extracted by an analog multiplexer 92, converted to a digital signal by an A/D converter 98, and then sent to a microprocessor 94. This microprocessor 94 calculates the analysis results and controls each part according to the tourogram stored in the H,0M95. The output device 96 includes, for example, a printer, a display, a CRT, etc., and outputs the analysis results. Note that reference numeral 97 is an input unit provided with various keys, and reference numeral 98 is a RAM.

FIG. 8 is a flowchart for calculating analysis results. The analysis value calculation is for quantifying a specific component present in the liquid sample based on the basic value measured by the basic value measuring section 89. In the correction value calculation, the output signal of the auxiliary value measuring section 43 is processed to use developed color density as an auxiliary value, and then a correction value is calculated from this auxiliary value. The auxiliary value and the correction value have a relationship as shown in FIG. 9A, for example. This relationship is determined in advance through experiments, and the obtained relationship is stored in the ROM 95.

The analysis value correction calculation is to correct the analysis value by, for example, multiplying or adding or subtracting the correction value to the analysis value, and the corrected analysis value is output as the analysis result.

In general, since the developed circle is almost circular, there is a correlation between the area and diameter of the developed circle, and there is also a correlation between the area of the developed circle and its color density, so both of these can be used as auxiliary values. Can be used. Further, in the reagent holding layer 6, #1 is colored in a circular shape, and since this colored circle is the same size as the developed circle, the area or diameter of the colored circle can also be used as an auxiliary value, as shown in Figure 9B. can.

FIG. 10 shows an embodiment of an auxiliary value measuring section that measures the expanded diameter or area as an auxiliary value. A COD image sensor is used as the photodetector 90, and the controller 100 reads out in time series the outputs of each element of the light receiving section of the COD onto which the developed layer is projected. The light source that illuminates the spreading layer is
Those used in the above embodiments may be used, and their explanation will be omitted. The signal read by the controller 100 is sent to the comparator 101
The presence/absence level of the sample is compared and classified in step a, and the sample detection signal is integrated by an integrator 101 to calculate the developed area or diameter. The resulting expanded area or diameter is sent to multiplexer 92. Microprocessor 94 via A/D converter 98
is input. If the signal read by the controller 100 is directly input to the A/D converter 98 and the microprocessor performs comparison, integration, and area calculation, the comparator 101a and the integrator 1
It goes without saying that 01 can be omitted. In addition,
Instead of COD, it is also possible to measure the diameter of the spread layer by using a line sensor in which light-receiving elements are arranged in a line so that the line sensor is located at the center of the spread layer.

FIG. 11 shows an embodiment in which the auxiliary value is measured before the sheet-like analysis element is loaded into the carrier. A housing 1 housing a light transmitting unit 104 having a fiber bundle, a light receiver and a light emitter.
05.

FIG. 12A is an explanatory diagram of the auxiliary value measuring section 108 shown in FIG. 11. In FIG. 12A, the auxiliary value measuring section 108 is connected to the projector 106. Optical fiber bundle 107° for guiding the light from the projector 106 Optical fiber bundle 109° for guiding the reflected light of the light projected onto the sheet analysis element 1 by the optical fiber bundle 107 to the receiver 108 Electricity is supplied to the projector 106 A cable 100 for extracting signals from the transmitter or receiver 108. and a housing 105. Optical fiber bundle 1
07 and 109, as shown in FIG.
091 Cylindrical optical fiber bundle 10 around the periphery
7 is provided. In the case of this embodiment, since the auxiliary value measuring section is located in a previous step than the basic value measuring section, the auxiliary value is measured before the basic value.

The light receiver 108 is a phototransistor.

A photodiode or the like is used, and its output is sent to an analog multiplexer 92.7 shown in FIG. A/D converter 9
8 and is input to the microprocessor 94. The microprocessor 94 compares and discriminates the presence/absence level of the sample as in the previous embodiment, and from the time length tθ of the signal and the speed V of the lever, the diameter D=t.

×v is noticed. When the insertion lever is fed by a pulse motor, the diameter can be determined by counting the amount of feeding, that is, the diameter, by the number of pulses during the sample presence signal period. Further, since the output of the light receiver 108 near the center of the diameter corresponds to the color density and is also an output, the microprocessor 94 can use it to calculate the color density and the correction value.

FIG. 12C is for explaining the output obtained by the light receiver 108 of the auxiliary value measuring section 108 when the sheet-like analysis element 1 is inserted into the storage section 22 from the insertion port 80 by the push lever 85. In the middle, ■ indicates the output level of the light receiver 108 by the upper slide frame 2 of the sheet-like analysis element 1;
"e" is the output level of the light receiver 108 from the developing layer 8 of the measuring element 4 in the hole 2a, and "e" is the time length of the output of the light receiver 108 from the part where the liquid sample is spotted on the developing layer 8 and developed. , ■ indicates the level for determining whether the liquid sample has been deposited and developed, and ■ indicates the output level of the light receiver 108 obtained at the center of the developed diameter.

In place of the auxiliary value measuring section shown in FIG. 11, FIG.
FIG. 3 is an explanatory diagram showing an example of measurement using a photodetector in which small light-receiving elements are arranged in a line.

The photodetector 110 includes, for example, 11 light-receiving element groups 110a to 110n arranged in a line in a direction perpendicular to the transport direction of the sheet-like analysis element 1, and detects reflected light from a light source that irradiates the developing layer. A light source designated by reference numeral 86 is used as the light source for measurement.

The filter may be on either the light source side or the light receiving side. When the sheet-shaped analytical element 1 is moved in the arrow direction at a constant speed V by the pushing lever 85, the developing layer 111 is moved into the light receiving element group 1103~
110n. As a result, signals as shown in FIG. 14 are output from the light receiving element groups 1103 to 110n.

Note that the horizontal axis is time.

FIG. 15 shows the electrical circuit used in the embodiment shown in FIG. 11 in an easy-to-understand manner. Each light receiving element group 110a to 1
Amplifiers 112a-112n are connected to 10n, respectively. The outputs of these amplifiers 112a to 112n are input to comparators 1183 to 1180, and the expansion layer 7 and the expansion layer 111 are discriminated. This comparator 118a-1
The output signal of 18n is shown in FIG. Each comparator] Time t when 18a to 118n are at rHJ]
~tn, the developed area is calculated from the following equation.

Development area "" (tl+t2+...1n)XvX! −
(1) Here, n is the number of light receiving elements 110a to 110n, and l is the length of the light receiving element group.

The summing integrator 114 inputs the outputs of each of the comparators 118a to 118n and substantially calculates the expanded area by calculating the equation (1). The obtained signal representing the developed area is sent to the analog multiplexer 92 and the A/D converter 9.
8 to the microprocessor 94. Along with this, it is also sent to the comparator 115, where it is determined whether the developed area is extremely small. If the developed area is extremely small, a warning output is sent to the microprocessor 94, and a warning is displayed on the output device 96. This warning display is made by displaying a message on a CRT, by printing a message on a printer, or by sounding a buzzer or the like. Note that the other configurations are the same as those shown in FIG. 7, so explanations will be omitted.

FIG. 17 is a flowchart of the embodiment shown in FIG. 15. In this embodiment, a reanalysis instruction is issued when a warning is output. When this reanalysis instruction is issued, the operator follows the instructions to dilute the sample to improve fluidity, and then spots the sample on the next new sheet-like analytical element. Perform a reanalysis. Note that when outputting the analysis results of the samples for which a warning has been issued, either a symbol indicating that the warning has been issued is added to the output, or the analysis results are not output. When performing re-analysis, analysis calculations, output of results, etc. are performed during the re-analysis. As shown in FIG. The input signal is input to the microprocessor 94 via the A/D converter 98, and the microprocessor 9
4 shows an embodiment in which comparison, integration, area calculation, and warning determination are performed, and the comparator 118, addition integrator 114, and comparator 115 are omitted. Amplifier 1: This amplifier is provided when a two-digit gain is required, and does not necessarily need to be provided.

It should be noted that it is obvious that if the signals of each photoreceiver group are compared, identified, and integrated when the photoreceiver group is located at the center of the expansion layer, the expansion can be directly determined. It shows another embodiment of the measuring section. In this embodiment, an auxiliary value measuring section 122 is attached to the cover 50, and includes a mounting base 128, and an illumination tube 124 and a light receiving tube 125 fixed to the mounting base 128. The structures of the illumination tube 124 and the light receiving tube 125 are the same as those shown in FIG. 6, so a detailed explanation thereof will be omitted. The carrier 28' used in this embodiment has an opening formed in its upper part, a transparent cover 126 is electrically connected to this opening, and the concentration or the developed diameter of the spread layer is measured through the transparent cover 126. It should be noted that if the above-mentioned COD or a line sensor using a group of light receiving elements is used as a light receiver, the development area will be the same as in the above embodiments.

FIGS. 20 and 21 show an embodiment of the auxiliary value measuring section 180 that measures the colored circle. Hole 18 in constant temperature plate 21
1 is formed, and a light transmitting member 184 including a light emitting optical fiber bundle 182 and a light receiving optical fiber bundle 188 is attached in this hole 181. A light source 185 is arranged at one end of the light emitting optical fiber bundle 182, and the light receiving optical fiber bundle 18
A photodetector 186 is arranged at B. In addition, as an auxiliary value measuring section, the above-mentioned COD or line sensor may be used to determine the development area.

As another example of measuring the coloring circle, the measurement area of the basic value measuring section may be made sufficiently smaller than the developed diameter, and then the basic value measurement part and the auxiliary value measurement may be used. In this case, the second
As shown in Fig. 2 and Fig. 28, at the color development circle of the sheet-like analytical element, at the corner of the storage part of the thermostatic plate, by a radius in the X direction,
Since only the radius is scanned in the X direction, the diameter can be determined by adding the length in the X direction and the length in the X direction. The basic measurement value is the diameter of the 9 colored circles measured at the center as an auxiliary value.

In the above example, the basic value and the auxiliary value are measured using visible light, but depending on the type of reagent and the properties of the liquid sample,
Ultraviolet light, infrared light, fluorescent light, etc. can be used. Furthermore, in order to measure the area, the reflectance can be measured for an area larger than the maximum value of the developed circle, and the area can be calculated from this reflectance. In this case, since the concentration is not constant depending on the sample, it is necessary to set an open chain for detection. .

〔Effect of the invention〕

In the present invention having the above configuration, a sheet-like analytical element is loaded into a carrier, moved on a constant temperature plate to perform incubation, and after this incubation, the concentration change or rate of change of the coloring circle is measured as a basic value, After spotting the liquid sample, at least one of the area and diameter of the developing circle, the developing circle σ color density, or the area and diameter of the coloring circle is measured as an auxiliary value, and the analysis results are corrected using this auxiliary value. Since the,
Correct analysis results can be obtained even for liquid samples in abnormal areas where the spread is insufficient.

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram showing an example of a sheet-like analytical element. FIG. 2 is an explanatory diagram showing the state of spotting a liquid sample. FIG. 8A is a perspective view showing the mechanical structure of the first embodiment of the present invention, and FIG. 8B is a cross-sectional view thereof. FIG. 4 is a plan view showing the drive mechanism of the push lever. FIG. 5 is a sectional view showing the configuration of the basic value measuring section. FIG. 6 is a sectional view showing the configuration of the auxiliary value measuring section. FIG. 7 is a block diagram showing the electrical configuration of the first embodiment of the present invention. FIG. 8 is a flowchart showing the analysis procedure. FIGS. 9A and 9B are graphs showing the relationship between the auxiliary value and the correction value. FIG. 10 is an explanatory diagram showing an embodiment of the auxiliary value measuring section. FIG. 11 is a perspective view showing the mechanical configuration of a second embodiment of the present invention. . FIG. 12A is an explanatory diagram showing the auxiliary value measuring section shown in FIG. 11, FIG. 12B is a sectional view of the light transmission section, and FIG. 12C is a waveform diagram of the output signal of the light receiver. be. FIG. 18 is an explanatory diagram showing a state in which the shape of an expanded circle is measured using a photodetector having light receiving elements arranged in a line. FIG. 14 is a waveform diagram showing the output of the light receiving element shown in FIG. 18. FIG. 15 is a block diagram showing the electrical configuration of a second embodiment of the present invention. FIG. 16 is a waveform diagram showing how the comparator shown in FIG. 15 is output. FIG. 17 is a flowchart showing the analysis procedure. FIG. 18 is a block diagram showing an embodiment in which the output of the light receiving element is electrically scanned. FIG. 19 is a sectional view showing another embodiment of the auxiliary value measuring section. FIG. 20 is a sectional view showing still another embodiment of the auxiliary value measuring section. FIG. 21 is an enlarged sectional view of the main part of FIG. 19. FIG. 22 is an explanatory diagram showing the moving direction in the constant temperature plate. FIG. 28 is an explanatory diagram showing the coordinates of the sheet-like analysis element. 1...Sheet-like analysis element 2.8...Slide frame 4...Measurement element 5...Transparent support 6...Reagent holding layer 7...Reflection layer 8...Development layer 9.・Liquid sample 20 ・Hot plate 21 ・Thermostatic plate 22 ・Storage section 28・l carrier 25-28 ・Pushing lever 80 ・Insertion port, 81 ・Loading table 88 ・・・Micropipette 85・Press lever 89...Basic value measuring section 84.86, 44.47...・Motor 40...Fist outlet 42...Stop claw 48...Auxiliary value measuring section 5
8...Cams 54-57...Follower 78.86...Light source 77.90...Photodetector 90...CCD image sensor 108...Auxiliary value measuring section 104...Light Transmission member 110...photodetector 110
a~110n e e *Light receiving element 111...Development layer 112...Amplifier 118...Comparator 117...Analog multiplexer 122...Auxiliary value measuring section 126...Transparent cover 180... Auxiliary value measuring section 182... Optical fiber bundle for light projection 188...
・Light receiving optical fiber bundle 185...Light source 1
86...Photodetector. Figure 8 Figure 10 4-1 Figure 9A Capture (area, diameter)

Claims (9)

[Claims] (1) A liquid sample of -wax amount is spotted on a sheet-like analysis element consisting of a reagent holding layer and a spreading layer provided on a transparent support, and the presence of the liquid sample in the liquid sample is determined by the reaction result of the reagent holding layer. a carrier into which the sheet-like analysis element is loaded from an insertion port formed on the front surface of the chemical analysis device for quantifying a test component; A thermostatic plate having a rectangular storage section that stores the carriers arranged in two rows. means for intermittently transporting the carrier together with the sheet-like analysis element at a constant pitch so as to circulate within the storage section; a first measuring means for optically measuring the reaction result of the sheet-like analytical element from the transparent support side; a second measuring means for measuring at least one of the developed area, developed diameter, developed color density in the developed layer of the sheet-like analytical element, or the reaction area or reaction diameter in the reagent holding layer; A chemical analysis device comprising calculation means for calculating a correction value from the measured value of the second measuring means and correcting the measured value of the first measuring means. (2) The chemical analysis apparatus according to claim 1, wherein the transfer means are push-in levers that are arranged at each of the four corners of the storage section and are sequentially operated. (3) The second measuring means is provided on a chute that guides the sheet-like analytical element discharged from the storage section, and the sheet-like analytical element is stopped by a stop claw attached to the chute. A chemical analysis device according to claim 1 or 2, characterized in that the chemical analysis device performs measurement. (4) The chemical analysis according to claim 1 or 2, wherein the second measuring means is provided at a loading position for loading the sheet-like analysis element into the carrier. Device. (5) The chemical analysis apparatus according to claim 1 or 2, wherein the second measuring means is attached to a constant temperature plate. (6) Claim 1, characterized in that the measuring means according to item 2 comprises a plurality of light receiving elements arranged in a line along a direction perpendicular to the moving direction of the light source and the sheet-like analysis element. The chemical analysis device according to any one of Items 1 to 5. (7) The chemical analysis device 1 according to any one of claims 1 to 5, wherein the second measuring means is a light source and a COD image sensor; (8) The chemical analysis device according to claim 1 or 2, wherein the carrier has a transparent upper surface. (9) The second measuring means is attached to a cover placed on a constant temperature plate, and the sheet-like analytical elements are individually determined through the carrier. The chemical analysis device according to item 8.