JPS60165535A - Instrument for measuring optical characteristic of sample - Google Patents

Abstract

PURPOSE:To enable photometry while a sample is not subjected to any chemical operation by measuring the transmittance of the sample by the photometric part provided in a transfer passage for transferring the sample to a reaction vessel. CONSTITUTION:A sampling pipe 5 is cleaned and is fed to a sampling position (a) where the top end of the pipe 5 is immersed into the sample in a sample vessel 3-3 and after a specified amt. of the sample is sucked and collected by a cylinder pump 15 for sample, the sample is fed t a photometric position (c). The sample in the pipe 5 is measured at the two wavelengths; the wevelength light corresponding to the analyzing item and the wavelength light near the same by a photometric part 10 in the position (c). The resepctive outputs corresponding to the absorbancy are fed time-dividedly to an arithmetic unit 11. The unit 11 determines the transmittance for the specific wavelength region light by calculation.

Description

Detailed Description of the Invention (Technical Field) The present invention relates to a measuring ring for measuring optical characteristics that change due to chyle, jaundice, hemolysis, etc. of a sample such as serum to be analyzed in a biochemical analyzer. It is about weight.

(Prior art) When analyzing a sample such as serum using a biochemical analyzer, it is necessary to correct measurement errors caused by chylosis, jaundice, hemolysis, and various other interfering chromogens in the sample to obtain an accurate measurement value. , the degree of influence of each analysis item is measured photometrically, and a correction value is calculated using this photometric output to obtain a correct analysis value.

In order to detect the degree of influence of chyle, jaundice, wIllh, etc. on a sample, for example, in Japanese Patent Publication No. 54-63785, when analyzing an analysis item that does not produce absorbance due to the target substance in the visible wavelength range, A technique has been disclosed for measuring the amount of interfering chromogen in a sample from changes in absorbance that occur in a wavelength range. This method is superior to the well-known sample blank method in that it does not require the use of a sample blank;
The amount of interfering chromogen can only be detected in the case of analysis items that use test solutions that do not react in the visible wavelength range.
Furthermore, if you only want to measure chyle, etc., you not only have to dispense reagents used to measure items that do not originally need to be measured, but you also have to dispense reagents that are optimal for measuring chyle, etc. There are disadvantages such as not being able to use things.

Furthermore, Japanese Patent Application Laid-Open No. 55-26454 describes a method of measuring the permeability of a sample by dipping the nozzle at the tip of a cylinder containing a liquid suction plunger into the sample, and moving the suction plunger to transfer the sample into the cylinder. A method is disclosed in which the sample is inhaled into a flat sample chamber provided and the transmittance of the sample is measured while the sample is accommodated in the flat sample chamber. However, since this method uses a flat sample chamber, it is not easy to completely aspirate and eject the sample. Therefore, not only is there a risk of contamination, but there are also disadvantages such as the fact that the sample that has been measured cannot be used for measurements for other analysis items.

(Object of the Invention) The object of the present invention is to solve the difficulties in the conventional methods for measuring the permeability of samples as described above, and to improve the permeability of samples subject to biochemical analysis due to chyle, jaundice, hemolysis, etc. It is an object of the present invention to provide an optical property measuring device for a sample that can efficiently and accurately measure the influence of the sample without any influence on the sample.

(Summary of the Invention) When the present invention is implemented in, for example, a colorimetric analyzer for biochemical testing, in the process of transferring a sample in a sample container to a reaction container, a photometer is installed in the transfer path. By photometrically measuring the optical properties of the sample, for example, the transmittance at wavelengths corresponding to the analysis item, using light of multiple wavelengths, we can measure the optical properties of the sample before it enters the reaction process for analysis. It measures the optical properties of

In other words, the sample optical property measuring device of the present invention includes a sample collection tube that sucks and collects a sample in a sample container, and a plurality of transmittances that measure the transmittance of the sample in a transport path for the sample collected by the sample collection tube. The present invention is characterized in that it consists of a photometric section configured and arranged so as to be able to perform photometry using wavelength light, and an arithmetic device that calculates the optical characteristics of the sample using the output of this photometric section.

(Example) The figure is a microcosm showing the main part of the configuration of an example in which the device of the present invention is applied to an automatic analyzer.

In the figure, 1 indicates a sample guide line of the analyzer, 2 indicates a reaction line, and along the sample guide line 1, a plurality of sample containers 3-1, 3-2, . . . In addition, the reaction vessels 4-1, 4-2... are transferred in the direction of arrow B, and the sample vessels 3-1.3-2... are at the collection position a, and the reaction vessels 4-1.4- 2... are configured to sequentially pause at the injection position.

Reference numeral 5 denotes a sample collection tube made of a colorless translucent material and having an arbitrary cross-sectional shape, such as a circular or rectangular cross-section. In this embodiment, the sample dispensing nozzle of the automatic analyzer is shared with its sample collection tube 5, and the sample container 3-3 is stopped at the sample collection position a.
At the photometric position C in the transfer path where a sample is collected by the sample collection tube 5 and transferred to the reaction vessel 4-3, which is stopped at the injection position, the sample collection tube 5 is temporarily stopped and transferred to the reaction vessel 4-3, which is stopped at the injection position. After the sample has already been discharged, it is automatically controlled by a control device (not shown) so that it is cleaned at cleaning position 1 (not shown) and then returned to the sample collection position (a) in preparation for the next sample collection. There is.

Photometric position C in the sample transfer path by the sample collection tube 5
The photometer 10 has a well-known configuration consisting of a light source lamp 6, a rotary filter device 8 equipped with a plurality of interference filters 7-1, 7-2, etc. having different transmission wavelength ranges, and a photoelectric conversion element 9. However, it is arranged so that the photoelectric conversion element 9 can obtain an output corresponding to the optical characteristics, for example, the transmittance, of the sample in the sample collection tube 5 temporarily stopped in the photometry section @C.

11 is a calculation for calculating accurate transmittance of the sample at the measurement wavelength using a detection signal corresponding to the transmittance of the sample at the photometric wavelength using the well-known two-wavelength method obtained by the photometry section 10. When the device of the present invention is implemented as an analyzer as in this embodiment, the calculation device 11 performs calculations such as chyle, jaundice, hemolysis, etc. that affect the measurement accuracy of each desired analysis item. The correction value and the accurate measurement value of the correction result may also be calculated by calculating the amount of the correction value.

In addition, 12 is a cleaning/diluting liquid container containing the cleaning/diluting liquid 13, 14 is a syringe pump for feeding the cleaning/diluting liquid 13 to the sample collection tube 5, 15 is a syringe pump for sucking and discharging the sample, 16 and 17 is a first and second on-off valve;
Reference numeral 18 indicates cleaning and diluting liquid supply pipes, and these pumps and on-off valves are also automatically controlled by a control device (not shown) in the same manner as the sample collection pipe 5.

In the configuration of the above-described embodiment, the first on-off valve 16 is closed, the second on-off valve is opened, the cleaning/dilution liquid is sucked by the syringe pump 14, the second on-off valve 11 is opened, and the first on-off valve 15 is opened. By opening the valve and pressing the syringe pump 14, a cleaning/dilution liquid is supplied to the sample collection tube 5 at a cleaning position (not shown) for cleaning, and then the first opening/closing valve 16 is opened to release the cleaning/dilution liquid. The sample collection tube 5 is then transferred to the sample collection position a shown in the figure, with the inside of the feed tube 18 filled with the liquid.

Next, the tip of the sample collection tube 5 is lowered at the sample collection position a, and the tip of the sample collection tube 5 is immersed in the sample in the sample container 3-3 that is staying at that position a, and a fixed amount of the sample is pumped by the sample syringe pump 15. After suctioning and sampling, it ascends, transfers the sample to the photometry section MC, and pauses. The sample in the sample collection tube 5 is photometered by the photometer 10 at the photometering position C using two wavelengths, one corresponding to the analysis item and the other nearby wavelength, as described above. The output is obtained, for example, in a time-division manner and sent to the arithmetic unit 11.

After photometry in this way, the sample collection tube 5 stopped at the photometry position 10 is transferred to the injection position on the reaction line 2, and by pressing the sample syringe pump 15, the sample collection tube 5 stopped at the photometry position 10 is moved to the injection position [b The sample in the sample collection tube 5 is discharged and injected into the reaction vessel 4-3.

After this injection is completed, the sample collection tube 5 is transferred to a cleaning position (not shown) and cleaned as described above, completing one dispensing operation and waiting for the next sample dispensing.

It should be noted that the 2
The photometric output due to the wave is, for example, temporarily stored in a memory within the calculation device 11 and used for a predetermined calculation. For example 2
Calculate the difference value of the wave photometry output to determine accurate transmittance for light in a specific wavelength range, or correction values necessary to correct the measured value of an analysis item using light in the specific wavelength range from the transmittance. can be used.

In the above embodiment, the photometry section 10 includes a rotating filter device WI8 provided between the light source lamp 6 and the sample collection tube 5, converts the light from the light source lamp 6 into monochromatic light, irradiates the sample, and converts the transmitted light into a photoelectron. Although an example has been shown in which the light is guided to the conversion element 9, the photometry section in the device of the present invention is not limited thereto. For example, the sample in the sample collection tube 5 is irradiated with direct light from the light source lamp 6, and the transmitted light from the sample is guided to a rotating filter device, where a desired wavelength range is selected and photoelectrically converted by the photoelectric conversion element 9. Alternatively, a diffraction grating may be arranged in place of the rotating filter device in this configuration, and a plurality of photoelectric conversion elements simultaneously perform photoelectric conversion for each predetermined wavelength range to obtain a photoelectric conversion output in a desired wavelength range. It can also be configured to be selectively taken out.

Furthermore, in the above embodiment, all of the sample in the sample collection tube 5 is discharged into the reaction container after photometry, but there is no need for analysis and measurement, and it is only necessary to measure the transmittance of the sample at a predetermined wavelength. If the sample container is suitable, the sample container may be returned to the original sample container as soon as the photometry section 10 completes photometry. That is, when the device of the present invention is implemented in an analysis device as in the above embodiment, the optical characteristics of the sample can be measured at the stage of transferring the sample from the sample container to the reaction container. Therefore, since the sample remains in its original state, there is no problem even if it is returned to the original sample container, and the sample can be used for other measurement items without wasting it.

(Effects of the Invention) As described above in detail, according to the apparatus of the present invention, the transmittance of the sample is measured by the photometric section provided in the transfer path for transferring the sample to the reaction container. Photometry can be performed without any chemical manipulation.

Therefore, in conventional measurement methods, when photometrically measuring a diluted sample, the chyle may change depending on how the diluent is selected, but with the device of the present invention, there is no need for a diluent or reagent solution. Therefore, not only does such a problem not occur, but the running cost is also reduced, and since the optical characteristics of the sample are measured using a two-wavelength method, the measurement accuracy is also high.

In addition, in an analyzer for biochemical tests, the present invention can be used to measure the amount of interfering chromogens such as chyle, jaundice, and hemolysis that affect analytical values. If implemented as a measuring device, it can not only greatly contribute to improving the accuracy of analytical measurements, but also for samples where analytical measurement is not required and only the optical properties need to be measured, it is possible to simply return the sample to the sample container after measurement. , since the sample can be used for other purposes, waste of the sample can be avoided.

[Brief explanation of the drawing]

The figure is a deadline showing the main part of the configuration of an embodiment of the device of the present invention applied to an automatic analyzer. 1...Sample guide line 2...Reaction line 3-1~
3-4...Sample containers 4-1 to 4-4...Reaction container 5...Sample collection tube 6...Light source lamp 7-1.
7-2...Interference filter 8...Rotary filter device 9...Photoelectric conversion element 10...Photometering section 11...Arithmetic device 12...Cleaning/dilution liquid container 13...Cleaning/
Diluent 14.15...Syringe pump 16, 17.
...Opening/closing valve 18...Feeding pipe.

Claims (1)

[Claims] 1. A sample collection tube for sucking and collecting the sample in the sample container;
A photometry section is arranged in the transfer path of the sample collected by the sample collection tube so that the transmittance of the sample can be photometered using light of a plurality of wavelengths; 1. An optical property measuring device for a sample, comprising an arithmetic device for calculating the optical properties of a sample.