JPS60188060A - Live mold powder of bacterium belonging to genus bifidobacterium, having high resistance to acid in stomach, and its preparation - Google Patents
Live mold powder of bacterium belonging to genus bifidobacterium, having high resistance to acid in stomach, and its preparationInfo
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- JPS60188060A JPS60188060A JP59042982A JP4298284A JPS60188060A JP S60188060 A JPS60188060 A JP S60188060A JP 59042982 A JP59042982 A JP 59042982A JP 4298284 A JP4298284 A JP 4298284A JP S60188060 A JPS60188060 A JP S60188060A
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- bifidobacterium
- acid
- powder
- live
- bacterium belonging
- Prior art date
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Abstract
Description
本発明は、胃酸に対する耐性が高く、しかも室温保存中
にも生菌数が殆んど減少しない高角数のビフィズス菌生
菌粉末、およびその製法に関するものである。
一般に、ビフィズス菌は有用な腸内細菌とさｈている。
しかしながらビフィズス菌を生菌粉末としたとき、凍結
障害や乾燥障害があり、また保存中の生菌数の減少が著
しるしく、更に、履用時の胃酸による生菌数減少が著し
るしく、活性の高い生菌粉末の製品を得ることはきわめ
て困）１ｕ７であった。
本発明者らは、凍結障害や乾燥障害を防止し、保存中の
生菌数の減少がなく、また、胃酸による生菌数の減少の
ないビフィズス菌生菌粉末をめて鋭意研党したところ、
本発明においてこれら目的を達成することができた。
本発明は、牛乳蛋白質の加水分解物、糖類、酵母エキス
及びオレイン酸を主成分とする食用乳化剤を含有する培
地でビフィズス菌を嫌気条件下で１）Ｈをほぼ一定にし
て培養し、得られだ菌体含有液に脱脂粉乳、澱粉分解物
、グルタミン酸ソーダ、アスコルビン酸ソーダ、マグネ
シウム塩を加え、凍結乾燥してなる胃酸耐性の高いビフ
ィズス菌生菌粉末及びその製造方法である。
本発明において生菌粉末にするビフィズス菌としてはヒ
ト由来のＢｉｆｉｄｏｂａｃｔｅｒｉｕｍ ｌｏｎｇｕ
ｍ 。
Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍ ｂｒｅｖｅ 、 Ｂ
ｉｆｉｄｏｂａｃｔｅｒｌｕｍｂｉｆｉｄｕｍ等のいず
れの菌株でもよい。例示として、Ｂｉｆｉｄｏｂａｃｔ
ｅｒｉｕｍ Ｉｏｎｇｕｍ ＡＴＣＣ１５７０７、Ｂｉ
ｆｉｄｏｂａｃｔｅｒｉｕｍ ｂｒｅｖｅ ＡＴＣＣ１
５７０Ｑ、Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍ ｂｉｆｉ
ｄｕｍ ＡＴＣＣ１１１４６などがある。
本発明においては、特定成分の培地を必要とし、かつ、
特定成分の凍結乾燥分散機を必要とし、こｈらの組合せ
およびｐ）１をほぼ一定に１．、て培養することによっ
てはじめて保存性がよく、胃酸耐性のあるビフィズス菌
生菌粉末が得られるのである。
培地としては、牛乳蛋白質の加水分解物、糖類、酵母エ
キス及びオレイン酸を主成分とする食用乳化剤がすべて
組合される。牛乳蛋白質の加水分解物としては脱脂乳、
ホエー、カゼインなどの蛋白型分解酵素による加水分解
物があげられる。糖類とし、てけ、各朴糖類があるが、
乳糖で十分である。
オレイン酸を主成分とする食用乳化剤とし、てはオレイ
ン酸モノグリセライド、ソルビタンオレイン酸モノエス
テルなどかあげら力る。
培地の成分中オレイン酸を主成分とする賞用乳化剤は、
ビフィズス生菌の胃酸耐性を付耳するのに特に重要なも
のである。
培地としては、牛乳蛋白質の加水分解物１〜１０チ程度
、糖類１〜７％程度、酵母エキス００１〜２チ程度、オ
レイン酸を主成分とする食用乳化剤ｏ、ｏｏｉ〜１．０
１程度が用いられる。
培地の■旧は使用するビフィズス菌の種類によってもわ
ずかづつ変化するが、約６，８〜４．７’４４［の間に
調整される。
この培地は１２０°Ｇ１０分間程度で加熱滅菌する。
ここでビフィズス菌稍菌を接種し、９素ガス又は炭酸ガ
スを辿気しながら約３７℃で嫌気培養を行う。培養中は
カセイソーダ等によりｐＨの変動を防止し、それぞわの
ビフィズス菌に適した１１１（を一定に維持するのが好
ましい。
約１２時間程度培養して略最高菌数に達するので培養を
とめ、培養液は遠心分離し、濃縮された菌体含有液を得
る。菌体を含有する培養液はその寸までもよいが、高菌
数の生菌を得るために濃縮するのが好脣Ｌ７い。
菌体含有液には脱脂粉乳、澱粉分解物、ショ糖、グルタ
ミン酸ソーダ、アスコルビン酸ソーダ、マグネシウム塩
を添力ＩＬＬ、−２０℃以下に凍結し、凍結乾燥する。
この際、マグネシウム塩の添加は生菌の凍結乾燥時の障
害を防止するのに必要なもので、塩化マグネシウム又は
硫酸マグネシウムなどの可溶性マグネシウム塩を用いる
。
これら分散媒はいす力もビフィズス生菌の凍結乾燥に必
要なもので、脱脂粉乳０．５〜２０チ、澱粉分解物０５
〜２０％、グルタミン酸ソーダ００１〜２チ、アスコル
ビン酸ソーダ０．０１〜２％、マグネシウムｉ０．００
１〜０．２ ’１６ Ｈ度づつ配合さり、る。
凍結乾燥物には、更に脱脂粉乳、澱粉分解物、アスコル
ビン酸ソーダ等を添加、混合して、保存中の生菌数の減
少を防止する。
次に本発明の試験例及び実施例を示す。
試験例
実施例１で製造し７た本発明のビフィズス菌生菌粉末の
胃酸耐性と６７℃及び２０℃による保存中の菌数低下を
試験した一０対照として市販のビフィズス菌生菌粉末の
市販品Ａ及び市販品Ｂを同時に試験した。
（１） 胃酸耐性
人工胃液（ｐ）１３．５）耐性は、試料を水に溶解し、
人工胃＃（０６％ＮａＣｌ、０．５５％はプシン５チＩ
Ｎ−ＨＣｌ、１＋Ｈ１，４）を加えて１）［Ｉを３．
５とする。
６７°６０分保持し、たのち、直ちにｐＨを６．５に調
整し、ビフィズス菌数を計測する。原菌数に対する生存
菌チで表示する。
人工胃液（ＩＩｌ］２５）耐性は、上記同様に操作する
。ただし反応！１１１を２．５とする。
試験の結果は次の第１表に示される。
（２）菌数低下
保存（３７℃及び２０℃）中の菌数低下は、１ケ月ごと
に５ヶ月オで、試料１ｇ中のビフィズス菌数を針側する
。
結果は第１図（３７℃保存）と第２図（２０℃保存）に
示される。
ここにおいて、１は実施例１にて製造したビフィズス菌
生菌粉末、Ａは市販品Ａ、Ｂは市販品Ｂを示す。
実施例１
脱脂粉乳１０Ｋｖを温水１００ Ｋ＞に溶解し、蛋白質
分解酵素粉末（微生物由来）４０ｇを加え、５０℃で６
時間反応させて牛乳蛋白質を加水分解する。これに酵母
エキス５００．９．オレイン酸モノグリセリド１００ｇ
を加え、田を６．５に調整する。１２１８Ｃ７分間加熱
し、酵素の不活性化と滅菌をあわせ行なう。冷却後ヒト
由来の
Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍ Ｉｏｎｇｕｍ を接
種し、炭酸ガスを通気しながら６７℃で嫌気培養を行な
う。培養に際しては滅菌した２ Ｎ −ＮａＯＨを滴下
してｌ）＋１を６．２〜６．５となるように調瞥する。
約１５時間培養したのち冷却Ｌ７、約１０’、ＯＤＤ
回転７分で遠心分離し、濃厚菌体部分的２０ Ｋｙを集
める。これに脱脂粉乳Ｉ Ｋｇ、酸分解澱粉５００ｇ、
グルタミン酸ナトリウム５０ｇ、アスコルビン酸ナトリ
ウム５０ｇを加え、さらにＭｇＣｌ２・６Ｈ，０４９を
少量・の水にとかして加える。−８０℃に凍結したのち
、凍結乾燥The present invention relates to a powder of viable Bifidobacterium bacteria that is highly resistant to gastric acid and has a high number of viable bacteria that hardly decreases even during storage at room temperature, and a method for producing the same. In general, Bifidobacteria are considered to be useful intestinal bacteria. However, when Bifidobacterium is made into a live bacteria powder, there are problems with freezing and drying, and the number of viable bacteria decreases significantly during storage.Furthermore, the number of viable bacteria decreases significantly due to stomach acid when worn. It is extremely difficult to obtain a viable bacterial powder product with high activity). The present inventors have conducted extensive research to develop a viable Bifidobacterium powder that prevents freezing damage and drying damage, does not reduce the number of viable bacteria during storage, and does not cause a decrease in the number of viable bacteria due to stomach acid. ,
The present invention has achieved these objectives. The present invention is produced by culturing bifidobacteria under anaerobic conditions in a medium containing milk protein hydrolyzate, saccharides, yeast extract, and an edible emulsifier containing oleic acid as the main components. This is a live Bifidobacterium powder with high resistance to gastric acid, which is obtained by adding skim milk powder, starch decomposition product, sodium glutamate, sodium ascorbic acid, and magnesium salt to a bacterial cell-containing liquid and freeze-drying the mixture, and a method for producing the same. In the present invention, the human-derived Bifidobacterium longu is used as a living bacteria powder.
m. Bifidobacterium breve, B
Any strain such as Ifidobacter lumbifidum may be used. By way of example, Bifidobact
erium Iongum ATCC15707, Bi
fidobacterium breve ATCC1
570Q, Bifidobacterium bifi
dum ATCC11146 etc. In the present invention, a medium with specific components is required, and
It requires a freeze-drying disperser for specific components, a combination of these and p) 1. Only by culturing the bacteria can we obtain live Bifidobacterium powder that has a good shelf life and is resistant to stomach acid. As the medium, a milk protein hydrolyzate, saccharides, yeast extract, and an edible emulsifier containing oleic acid as a main component are all combined. Examples of milk protein hydrolysates include skim milk,
Examples include hydrolysates of whey and casein using proteolytic enzymes. There are various types of sugars, such as sugars and sugars,
Lactose is sufficient. Edible emulsifiers whose main ingredient is oleic acid, such as oleic acid monoglyceride and sorbitan oleic acid monoester. The prize emulsifier whose main component is oleic acid among the ingredients of the medium is
This is particularly important in determining the gastric acid resistance of Bifidobacteria. As a medium, milk protein hydrolyzate is about 1 to 10%, saccharide is about 1 to 7%, yeast extract is about 001 to 2%, and an edible emulsifier whose main component is oleic acid is o, ooi to 1.0%.
1 is used. The age of the medium varies slightly depending on the type of Bifidobacterium used, but is adjusted to between about 6.8 and 4.7'44. This medium is heat sterilized at 120°G for about 10 minutes. Here, Bifidobacterium bacterium is inoculated, and anaerobic culture is carried out at about 37° C. while passing 9 elemental gas or carbon dioxide gas. During culturing, it is preferable to prevent pH fluctuations with caustic soda, etc., and maintain a constant pH of 111 (which is suitable for each type of bifidobacteria). After culturing for about 12 hours, the maximum number of bacteria is reached, so stop culturing. , the culture solution is centrifuged to obtain a concentrated bacterial cell-containing solution.The culture solution containing bacterial cells can be as small as that, but it is preferable to concentrate it to obtain a high number of viable bacteria. Add skim milk powder, starch decomposition product, sucrose, sodium glutamate, sodium ascorbate, and magnesium salt to the bacterial cell-containing liquid, freeze to below -20°C, and lyophilize.At this time, the magnesium salt is The addition is necessary to prevent damage during freeze-drying of live bacteria, and soluble magnesium salts such as magnesium chloride or magnesium sulfate are used. , skimmed milk powder 0.5-20 t, starch decomposition product 05
~20%, Sodium glutamate 001~2%, Sodium ascorbate 0.01~2%, Magnesium i0.00
Contains 1 to 0.2'16 H degrees each. Skim milk powder, starch decomposition product, sodium ascorbic acid, etc. are further added and mixed with the freeze-dried product to prevent the number of viable bacteria from decreasing during storage. Next, test examples and examples of the present invention will be shown. Test Example The Bifidobacteria live bacteria powder of the present invention produced in Example 1 was tested for gastric acid resistance and bacterial count reduction during storage at 67°C and 20°C.10 Commercially available Bifidobacterium live bacteria powder as a control. Product A and commercial product B were tested simultaneously. (1) Gastric acid resistance Artificial gastric fluid (p) 13.5) Resistance is determined by dissolving the sample in water,
Artificial stomach # (06% NaCl, 0.55% Pusin 5 Chi I
Add N-HCl, 1+H1,4) to 1) [I to 3.
5. After holding at 67° for 60 minutes, the pH was immediately adjusted to 6.5 and the number of bifidobacteria was counted. It is expressed as the number of viable bacteria compared to the number of original bacteria. The artificial gastric juice (IIl)25) resistance is operated in the same manner as above. However, reaction! 111 is set to 2.5. The results of the test are shown in Table 1 below. (2) Decrease in Bacterial Count During storage (37°C and 20°C), reduce the number of Bifidobacteria in 1 g of sample every 5 months. The results are shown in Figure 1 (stored at 37°C) and Figure 2 (stored at 20°C). Here, 1 indicates the live Bifidobacterium powder produced in Example 1, A indicates commercial product A, and B indicates commercial product B. Example 1 10Kv of skim milk powder was dissolved in 100K warm water, 40g of proteolytic enzyme powder (derived from microorganisms) was added, and the mixture was heated at 50℃ for 6 hours.
Milk proteins are hydrolyzed by a time reaction. Add to this yeast extract 500.9. Oleic acid monoglyceride 100g
Add and adjust the value to 6.5. Heat to 1218C for 7 minutes to inactivate the enzyme and sterilize it. After cooling, human-derived Bifidobacterium Iongum was inoculated, and anaerobic culture was performed at 67° C. while aerating carbon dioxide gas. During culturing, sterilized 2N-NaOH is added dropwise and the value of l)+1 is checked to be 6.2 to 6.5. After culturing for about 15 hours, cool L7, about 10', ODD
Centrifuge for 7 minutes to collect a partial 20 Ky of concentrated bacterial cells. Add to this 1 kg of skim milk powder, 500 g of acid-decomposed starch,
Add 50 g of sodium glutamate and 50 g of sodium ascorbate, and then add MgCl2.6H,049 dissolved in a small amount of water. Freeze-dry after freezing to -80℃
【−１乾燥物約３．２ Ｋｇを得る。粉砕し
、こねに脱脂粉乳Ｉ Ｋｇ、酸分解澱粉Ｉ Ｋｙ、アス
コルビン酸ナトリウム５０ｇを加え混合し、ビフィズス
菌生菌粉末とする。
との生菌粉末Ｉｒ１１．！ｉ＋当りビフィズス菌１０ｕ
以上を含有し胃酸剛性、および保存性ともすぐれていた
。
実施例２
ナトリウムカゼイン３Ｋｆに熱水１ｏｏＫｆを加えて溶
解する。微生物由来の蛋白質分解酵素粉末４０ｇを加え
、５０℃で４時間反応させてカゼインを加水分解する。
こわに乳糖５Ｋｆ、酵母エキス５００＆、ソルビタンオ
レイン酸モノエステル５０ｇ、を加え１）１（を６．０
に調整する。１２０°、１０分間加熱し、滅菌と酵素の
不活性化とをあわせ行なう。
冷却後ヒト由来のＢｉｆｉｄｏｂａｃｔｅｒｉｕｍ Ｉ
ｏｎｇｕｍ を接種し、窒素ガスを通気しながら３７℃
で嫌気培養を行なう。培養に際して滅菌した１ ０ ％
ＮａＯＨを滴下して１１）］を６．０近傍に調整する
。約１２時間培養したのち冷却し、６．ＯＤＤ回転／分
で遠心分離し、濃厚菌体部分的！ｌ Ｏｘ９を得る。こ
れに酸分解澱粉ｉ ＫＦ、脱脂粉乳６００ｇ、グルタミ
ン酸ナトリウム６０ｇ、アスコルビン酸ナトリウム５０
ｇを加え、さらにＭｇ５Ｏ，・７Ｈ２０５，９を少量の
水にとかして加える。−３０℃に凍結したのち、凍結乾
燥し、乾燥物的６．０ Ｋｇを得る。粉砕し、これに脱
脂粉乳５００ｇ、酸分解澱粉ｓｏｏｇ、アスコルビンＭ
１００ｇを加えてビフィズス菌生菌粉末とする。
この生菌粉末ｄ’ｌ、９Ｍリビフイズス菌を１０１１以
上含有し、胃酸耐性および保存性共すぐれていた。
実施例６
ホエー粉１０Ｋｇに温水１ｏｏＫｆを加えて溶解する。
微生物由来の蛋白質分解酵素粉末４０ｇを加え、５０℃
で６時間反応させてホエー蛋白質を加水分解する。こわ
に酵母エキス５００ｇ、ソルビタンオレイン酸モノエス
テル２５ｇ、オレイン酸モノグリセリド４０．ｌｉｔを
加え−を５．乙に調整する。
１２０°Ｃ８分間力Ｉノ熱し、滅菌と酵素の不活性化と
をあわせ行なう。冷却後、ヒトの乳児由来の１１目ｉｄ
ｏｂａｃｔｅｒｉｕｍ ｂｒｅｖｅ を接種Ｌ２．５７
℃で嫌気培養する。培養に際しては、滅菌した２Ｎ−Ｎ
ａＯＨを滴下して）】Ｈを５．５近傍に調整する。約５
時間培養後冷却し、脱脂粉乳１〜、酢分解澱粉１に９、
グルタミン酸ナトリウム５０ｇ、アスコルビン酸ナトリ
ウム５０．９を加え、さらにＭｇ８０．・７７Ｈ２Ｏ１
０を少量の水にとかし、て加える。−２０℃に凍結した
のち、凍結乾燥し、乾燥物５．０ Ｋｇを得る。粉砕し
、これに脱脂粉乳ＩＫｇ、酸分解澱粉ｉＫｇ、アスコル
ビン酸５００ｇを加え、ビフィズス菌生菌粉末とする。
この生菌粉末は１ｇ当りビフィズス菌を２ Ｘ １０’
以上含有し、胃酸耐性および保存性ともすぐれていた
。[-1 Approximately 3.2 Kg of dry matter is obtained. After crushing, add 1 kg of skim milk powder, 1 Ky of acid-decomposed starch, and 50 g of sodium ascorbate to the dough and mix to obtain live bifidobacterium powder. Live bacteria powder Ir11. ! Bifidobacterium 10u per i+
It contained the above ingredients and had excellent gastric acid resistance and preservability. Example 2 10Kf of hot water is added to 3Kf of sodium casein to dissolve it. Add 40 g of proteolytic enzyme powder derived from microorganisms, and react at 50° C. for 4 hours to hydrolyze casein. Add 5Kf of lactose, 500g of yeast extract & 50g of sorbitan oleate monoester to the stiffness and mix 1) 1(6.0
Adjust to. Heat at 120° for 10 minutes to both sterilize and inactivate the enzyme. Bifidobacterium I from humans after cooling
ongum was inoculated and heated at 37°C while aerating nitrogen gas.
Perform anaerobic culture. 10% sterilized during cultivation
11)] to around 6.0 by dropping NaOH. After culturing for about 12 hours, it was cooled, and 6. Centrifuge at ODD rotation/min to partially remove concentrated bacterial cells! lOx9 is obtained. Add to this acid-decomposed starch i KF, 600 g of skim milk powder, 60 g of sodium glutamate, and 50 g of sodium ascorbate.
g, and then dissolve Mg5O,.7H205,9 in a small amount of water and add. After freezing to -30°C, it is freeze-dried to obtain a dry weight of 6.0 kg. Grind and add 500g of skim milk powder, acid-decomposed starch soog, and ascorbic M.
Add 100 g to obtain live Bifidobacterium powder. This viable bacteria powder d'l contained 1011 or more of 9M Ribifidus bacteria and had excellent gastric acid resistance and storage stability. Example 6 10Kf of warm water was added to 10Kg of whey powder to dissolve it. Add 40g of proteolytic enzyme powder derived from microorganisms and heat at 50°C.
to hydrolyze whey protein by reacting for 6 hours. Kowani yeast extract 500g, sorbitan oleate monoester 25g, oleic acid monoglyceride 40. Add lit and -5. Adjust to Party B. Heat to 120° C. for 8 minutes to sterilize and inactivate the enzyme. After cooling, 11 eyes id from human infants
Inoculated with obacterium breve L2.57
Culture anaerobically at °C. When culturing, use sterilized 2N-N
Add aOH dropwise to adjust H to around 5.5. Approximately 5
After incubation for an hour, cool, skim milk powder 1 to 1, vinegar-decomposed starch 1 to 9,
Add 50 g of sodium glutamate, 50.9 g of sodium ascorbate, and further add 80.9 g of Mg.・77H2O1
Dissolve 0 in a small amount of water and add. After freezing to -20°C, it is freeze-dried to obtain 5.0 kg of dried product. The mixture is pulverized, and 1 kg of skim milk powder, 1 kg of acid-decomposed starch, and 500 g of ascorbic acid are added thereto to obtain live bifidobacterium powder. This live bacteria powder contains 2 x 10' bifidobacteria per gram.
It had excellent gastric acid resistance and storage stability.
か１図は試験例で３７℃保存の菌数低下を計測し、た図
で、第２図は試験例′Ｔ：２０′Ｇ保存の菌数低下を計
測した図である。
１・・・実施例１にて製造し２だビフィズス菌生菌粉末
、Ａ 市販品Ａ、 Ｂ・・・市販品Ｂ０代理人 弁肌士
戸 １）親 男Figure 1 shows the measurement of the decrease in the number of bacteria in the test example stored at 37°C, and Figure 2 is a diagram in which the decrease in the number of bacteria was measured in the test example 'T: 20'G storage. 1... Bifidobacteria live bacterial powder produced in Example 1, A Commercial product A, B... Commercial product B0 Agent Benhadashi 1) Parent Male
Claims (2)
オレイン酸を主成分とする食用乳化剤を含有する培地で
ビフィズス菌を嫌気条件下で−をほぼ一定にして培養し
、得られた菌体含有液に脱脂粉乳、澱粉分解物、グルタ
ミン酸ソーダ、アスコルビン酸ソーダ、マグネシウム塩
を加え、凍結乾燥してなる胃酸耐性の高いビフィズス菌
生菌粉末。(1) Bifidobacterium was cultured under anaerobic conditions in a medium containing a milk protein hydrolyzate, saccharides, yeast extract, and an edible emulsifier containing oleic acid as its main components, with a constant concentration of -. Bifidobacterium live bacteria powder with high resistance to stomach acid is obtained by adding skim milk powder, starch decomposition product, sodium glutamate, sodium ascorbic acid, and magnesium salt to body fluid and freeze-drying the mixture.
オレイン酸を主成分とする食用乳化剤を當侑する培地で
ビフィズス菌を嫌気条件下で−をはぼ一定にして培養し
、得られた菌体含肩液に脱脂粉乳、澱粉分解物、グルタ
ミン酸ソーダ、アスコルビン酸ソーｌ、マグネシウム塩
を加え、凍結乾燥することを特徴とする胃酸耐性の晶い
ビフィズス菌生菌粉末の製造方法。(2) Bifidobacterium was cultured under anaerobic conditions in a medium containing an edible emulsifier containing a milk protein hydrolyzate, saccharides, yeast extract, and an oleic acid as its main components, with a constant - content. A method for producing a crystalline live Bifidobacterium powder resistant to gastric acid, which comprises adding skim milk powder, starch decomposition product, sodium glutamate, sol ascorbic acid, and magnesium salt to a bacterial cell-containing fluid and freeze-drying the mixture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59042982A JPS60188060A (en) | 1984-03-08 | 1984-03-08 | Live mold powder of bacterium belonging to genus bifidobacterium, having high resistance to acid in stomach, and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59042982A JPS60188060A (en) | 1984-03-08 | 1984-03-08 | Live mold powder of bacterium belonging to genus bifidobacterium, having high resistance to acid in stomach, and its preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60188060A true JPS60188060A (en) | 1985-09-25 |
| JPH0416145B2 JPH0416145B2 (en) | 1992-03-23 |
Family
ID=12651239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59042982A Granted JPS60188060A (en) | 1984-03-08 | 1984-03-08 | Live mold powder of bacterium belonging to genus bifidobacterium, having high resistance to acid in stomach, and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60188060A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0195726A (en) * | 1987-08-21 | 1989-04-13 | Munk Werner Georg | Reconstructive dryed product for direct ingestion and method for its manufacture |
| US6368591B2 (en) | 1998-05-15 | 2002-04-09 | Shanghai Sine Pharmaceutical Corporation Ltd. | Beneficial microbe composition, new protective materials for the microbes, method to prepare the same and uses thereof |
| WO2005054443A1 (en) * | 2003-12-03 | 2005-06-16 | Aventis Pasteur Limited | Cryo-protective agents for microorganisms |
| WO2006052148A1 (en) * | 2004-11-15 | 2006-05-18 | Tine Ba | Process for preparing a bacterial culture, and the product prepared by the process |
| JP2008520201A (en) * | 2004-11-15 | 2008-06-19 | ティネ ビー エー | Method for preparing fermented products and products prepared by the method |
| JP2008267431A (en) * | 2007-04-17 | 2008-11-06 | Ntn Corp | Double row tapered roller bearing device |
-
1984
- 1984-03-08 JP JP59042982A patent/JPS60188060A/en active Granted
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0195726A (en) * | 1987-08-21 | 1989-04-13 | Munk Werner Georg | Reconstructive dryed product for direct ingestion and method for its manufacture |
| US6368591B2 (en) | 1998-05-15 | 2002-04-09 | Shanghai Sine Pharmaceutical Corporation Ltd. | Beneficial microbe composition, new protective materials for the microbes, method to prepare the same and uses thereof |
| WO2005054443A1 (en) * | 2003-12-03 | 2005-06-16 | Aventis Pasteur Limited | Cryo-protective agents for microorganisms |
| WO2006052148A1 (en) * | 2004-11-15 | 2006-05-18 | Tine Ba | Process for preparing a bacterial culture, and the product prepared by the process |
| JP2008520202A (en) * | 2004-11-15 | 2008-06-19 | ティネ ビー エー | Bacterial culture preparation method and product prepared by the method |
| JP2008520201A (en) * | 2004-11-15 | 2008-06-19 | ティネ ビー エー | Method for preparing fermented products and products prepared by the method |
| JP2008267431A (en) * | 2007-04-17 | 2008-11-06 | Ntn Corp | Double row tapered roller bearing device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0416145B2 (en) | 1992-03-23 |
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