JPS60187862A - Automatic analyzer - Google Patents

Abstract

PURPOSE:To detect the reacting conditions of a serum sample and a reagent simultaneously in a direct manner by interposing an optical measuring device employing an optical transmission fiber in a reaction tube transfer path having the positions of distributing the serum sample and the reagent to optically check a sample blank of the serum sample. CONSTITUTION:Both ends of an optical fiber 41 for emission of an optical measuring device 40 are arranged properly between the positions of distributing a serum sample and the first reagent. Light L of a light source received at the light receiving side end thereof is irradiated to a reflector 44 arranged in a measuring turret passing through an optical fiber 42 for reception enters a spectroscope 32. The measurement with the reflector 44 is performed between measurements of a reaction tube 5' and the subsequent reaction tube 5' and the detection of the sample black between the measuring times of the reaction tube 5' and the subsequent reaction tube 5'.

Description

[Detailed Description of the Invention] (Technical Field) The present invention relates to an automatic analyzer that performs biochemical analysis and immunological analysis. ), reagent blank,
This invention relates to an automatic analyzer that can perform cell blank analysis at the same time as normal analysis.

(Conventional example) When colorimetrically measuring blood analysis such as biochemical tests using optical means, the most important factor that causes loss of colorimetric measurement accuracy is the presence of the sample blank in the serum. If it can be determined whether or not this specimen blank is contained in serum, the reliability of measurement accuracy will be dramatically improved.

For this reason, conventionally, some automatic analyzers of this type are equipped with a mechanism for measuring sample blanks.

However, with this type of automatic analyzer, since the amount of serum sample dispensed into the reaction tube is extremely small, it is not possible to use a normal optical measuring device to directly observe the serum. At present, a dedicated optical measuring device for detecting sample blanks has been developed and equipment or a dedicated channel is used. This not only makes the entire device complicated and large, but also causes problems such as poor economic efficiency and complicated data processing.

(Object of the invention) This invention was created in view of the current situation, and
The aim is to create a simple configuration that can measure sample blanks using a spectrometer used for optical measurements to measure the reaction state between serum samples and reagents, and can also measure reagent blanks and cell blanks. The aim is to provide an automatic analyzer that does not require a dedicated sample blank measuring device.

(Structure of the Invention) In order to achieve the above object, the present invention is configured such that a required amount of a reagent corresponding to a measurement item is dispensed into a serum sample at a required position, and the reaction state is measured using an optical device. An optical measuring device using an optical transmission fiber is installed in the reaction tube transfer path having the serum sample dispensing position and reagent dispensing position of the automatic analyzer, and this optical measuring device optically measures the sample blank of the serum sample. It is configured to detect

(Example) Hereinafter, the present invention will be described in detail based on an example shown in the accompanying drawings.

The automatic analyzer according to this example [17 is based on this invention,
This is applied to an automatic biochemical analyzer, and as shown in FIG. 11 containers are arranged, including 10 containers containing serum samples for comparison and 1 container containing serum samples for comparison). ), a pipette device (6) that aspirates a predetermined amount of the serum sample from the general sample sample cartridge (3) at a predetermined position and dispenses it into the reaction tube (5), and a plurality of the reaction tubes (5). A pre-reaction turret (force) that can rotate 360 degrees while holding the main body and rotating intermittently, and a first reaction corresponding to the measurement item A first reagent device (QO) formed by removably mounting a container (8) containing a reagent on a turret plate (9), and a dispenser for dispensing a predetermined amount of the first reagent into a reaction tube (5). Note that the serum sample into which the first reagent has been dispensed is transferred from a predetermined position to another reaction tube (5 A change device (0th floor) for weighing and dispensing a predetermined amount into a measurement turret (121), and a container ( 8′) on a turret plate (9′) in a removable manner; a dispensing device 0-tan for dispensing a predetermined amount of the second reagent at a predetermined position; The reaction tube (5') into which is dispensed is transferred to the change device u3 mentioned above.
The reaction tube held in 021 (the above-mentioned measurement turret) is lifted up in synchronization with 1 and stirred by means such as ultrasonic vibration. A cleaning device (18) for cleaning the reaction tube (5') after the measurement has been completed in the device 0η, and the pipette device (6).
dispensing position (a) of the serum sample and dispensing the first reagent,
Dispensing position (a) and first reagent dispensing position (
There is no need to interpose it between the reactor and b), and it may be located anywhere within the reaction tube transfer path.

Furthermore, the measurement turret 021 is configured to be rotated 3600 times during one intermittent rotation in synchronization with the operation of the change device 0, so that the reaction tubes (5 of which are measurement turrets) 021 The same reaction tube (
The specimen in 5') is configured to be measured multiple times by the optical device αη.

Furthermore, the pre-reaction talent (force) also operates in a similar manner by shifting the rotational phase.

The optical device (17) includes a light source (1) and a measurement light (8) from this light source (1) which is focused and irradiated onto the reaction tube (5 lenses).The light is divided into wavelengths and arranged on a Rowland circle. The light is received by the detection element (34) for the desired wavelength.

On the other hand, the measuring device (4) for optically measuring the sample blank is composed of an optical fiber for light output (41) and an optical fiber for light reception (42), and one end of the optical fiber for light output (41) is connected to the optical device (4). 17)
The end (41a) on the light output side is located at the same position in order to irradiate the light source 00) to the reaction tube (5) at the desired position held by the sending device (force). It is located in
Further, the light-receiving side end (42a) of the light-receiving optical fiber (42) is connected to the light-emitting side end (41a) of the light-emitting optical fiber (41) by sandwiching the reaction tube (5) as shown in FIG. They are arranged so that they face each other. That is, both ends (41a) and (42a) are disposed at appropriate positions between the serum sample (g) dispensing position (a) and the first reagent dispensing position (b).

At least the light-emitting end (4ta) and the light-receiving end (42a) of each of these fibers (41, 42) are formed in the shape of a flat plate slit, as is clear from FIG. The reason why both ends (41a) and (42a) are formed into a flat plate slit shape is because the amount of serum sample (g) to be dispensed into the reaction tube (5) is extremely small, so it can be used as an efficient light source. This is to irradiate or receive the light (G) onto the serum sample (effect).
42), the light is irradiated onto a reflecting mirror (44) disposed within the measurement turret, and then enters the spectrometer (32).

As is clear from FIG. 1, the reflecting mirror (44) is
11j constant turret (interposed between the reaction tube holding parts of 121, and the light source light 0 is connected to the corresponding reflecting mirror (4) at the measurement position.
4) and is guided to the spectrometer (32). In other words, the measurement using this reflecting mirror (44) is performed using the reaction tube (
5 and the next reaction tube (5 measurements), and the detection of the sample blank by the light source light (G) is done at the same time as the measurement time of the reaction tube (5') and the next other reaction tube (5'). The sample blank data (no.
) is displayed during the phase with the measurement data (Dl) of the reaction tube (5/), as shown in FIG.

(Each data (Dz and data (Dl), and selects only the necessary information (wavelength signals corresponding to measurement items).As shown in Fig. 1, the α sickle consists of a logarithmic converter (2) and An A/D converter t21) that converts the analytical data input to the logarithmic converter 4 into a digital signal, and a storage circuit that stores the digital signal input to the A/D converter.
A microcomputer (c), a holding circuit (241) that holds the memorized measurement data (Dl) measured multiple times for each measurement sample, and a holding circuit (241) that holds the measurement data (Dl) that has been stored and measured multiple times for each measurement sample, and a holding circuit (241) that stores this analysis data over the time course of the same measurement sample after the measurement is completed. A seven-unit CRT that displays each sample for the same measurement, and a recording device (2!
It is composed of I.

Therefore, in the automatic analyzer (1) according to this embodiment, the reaction state between the serum sample (2) and the reagent is measured using the reflector mirror using the blank time between colorimetric measurements using the optical device a force. (44
) on the measurement turret for special reflection@
(44) Detecting milk and sample blanks such as hemolysis and yellow stains without synchronized automatic control can be performed with a single light source (3 and a single spectrometer Cl2), and this result can be By using it for data correction, it is possible to significantly improve the reliability of measurement accuracy. Furthermore, since the pre-reaction talent coating moves 360°, it is possible to measure not only sample blanks but also reagent blanks and cell blanks.

In the above embodiment, the light source measurement cost (4) is used in common with the optical device (1η light source (to)), but the present invention is not limited to this. Instead, another light source device may be used.

Alternatively, the light source device may be placed somewhere other than the measurement turret 021 and the light may be guided to the reaction tube (5') using an optical fiber, similar to the sample blank measurement optical system.

Furthermore, in the embodiment, the optical device Uη and the light source measuring device (
Although the explanation has been given by taking as an example the case where the method of 4 is applied to an automatic analyzer having separate rotary tables, the present invention is not limited to this, and of course can be applied to an automatic analyzer with a single rotary table type. It can be applied to all other types of automatic analyzers with appropriate changes, and can also be applied to automatic immunoanalyzers.

(Effects of the Invention) According to the present invention, as explained above, a minute amount of serum sample (several μp to several tens of μk) is directly reacted with the serum sample and reagent without using a dedicated optical measuring device for sample blank measurement. Since the condition can be detected simultaneously with a single spectrometer, the configuration is simple and economical.Using this specimen blank data greatly improves the reliability of the measured data. can do. Furthermore, since reagent blanks and cell blanks can be measured simultaneously, this can greatly contribute to improving the accuracy of measurement results.

[Brief explanation of the drawing]

The drawings are schematic explanatory diagrams showing the configuration of an automatic analyzer according to an embodiment of the present invention, FIGS. 2A and B are explanatory diagrams showing the optical transmission path in the reaction tube irradiation section, and FIG. 3 is a diagram showing the detection of a sample blank. FIG. 4 is a graph diagram showing an example of display of measurement data of sample blank and reaction status data of serum specimen and reagent, and FIG. 5 is a block diagram of the signal processing device. be. fi+...Automatic analyzer (51, (5')...Reaction tube aη...Optical device (to)...Light source (32)
...Spectrometer (40)...Optical measuring device (41)...
・Optical fiber for light output (42)...Optical fiber for light reception (41a)...Light output side end (4
Za)...Receiving side end Ce)...Measuring light (L)...
...Light source light (G)...Ume pickled sample patent applicant Nippon Techtron Co., Ltd. Figure 2 42 Figure 3 B Figure 4 Figure 5

Claims (1)

[Scope of Claims] ■) An automatic analyzer configured to dispense a required amount of a reagent corresponding to a measurement item into a serum sample at a predetermined position and measure the reaction state with an optical device. An optical measuring device using an optical transmission fiber is installed in the reaction tube transfer path which has a dispensing position and a reagent dispensing position. An automatic analysis device configured to optically detect marks. 2) The automatic analyzer according to claim 1, wherein the output side end and the light receiving side end of the optical transmission fiber are formed into a flat slit shape and are disposed opposite to each other. 3) Optical measurement of a serum sample using an optical measuring device is performed using an optical measuring device to determine the reaction state between the serum sample and a reagent.
- Hicho Rumor; 14 The measurement of the six members is configured so that it can be measured through a reflecting mirror provided between the reaction tubes of the measurement turret. The automatic analyzer according to item 2.