JPS60166009A - Treatment of polycyclic organic substance - Google Patents

Abstract

PURPOSE:To adsorb and desorb a polycyclic organic substance selectively using animal fiber. CONSTITUTION:A polycyclic org. substance, particularly, a mutagenic substance, comprising 3-amino-1,4-dimethyl-5H-pyrido-[4,3-b]indole, etc. is adsorbed and desorbed using animal fiber such as wool, silk, etc. The adsorption of said polycyclic org. substance to animal fiber is carried out by adding the animal fiber to a soln., particularly, aq. soln. contg. said polycyclic org. substance, and stirring or shaking at, usually, 0-100 deg.C, etc. To desorb the polycyclic org. substance adsorbed to the animal fiber, a solvent such as methanol, etc. is used, and the mixture is stirred or shaken at the boiling point of the solvent, etc.

Description

[Detailed description of the invention] It concerns methods of processing substances.

In recent years, mutagenic substances present in trace amounts in the environment, food, etc. have been attracting attention as the mortality rate due to cancer has increased. For this reason, the development of separation and concentration technology for removing these substances and researching their effects on humans has become an extremely important issue.

As a treatment method useful for selective adsorption removal and desorption concentration of such mutagenic substances, Japanese Patent Application Laid-Open No. 58-47001
The method described in Japanese Unexamined Patent Publication No. 58-170506 is known. All of these use phthalocyanine compounds as adsorbents.

The present inventors investigated adsorbents that selectively adsorb mutagenic substances and found that animal fibers are effective.

The present invention is a method for treating polycyclic organic substances, which is characterized by adsorbing polycyclic organic substances using animal fibers and desorbing them if necessary.

In the present invention, the polycyclic organic substance is an aromatic compound or a heterocyclic compound having two or more rings, and specific examples include the following compounds.

Trp-P-1(8-7mi/-1,4-dimethyl-5
H-pyrido(4,8-b]zeynol), Trp-P-
2(8-'7E,/-1-methyl-5H-pyrido(4,
8-b) indole), Glu-P-1 (2-amino-6-methyl-dipyrido(1,2-a:8,2-d)E-dazole), Glu-P-2 (2-7E/-' ) PyriF (1,2-a: 3', 2'-d) imidazole), amino-α-carboline (2-amino-9H
-pyrido(2,8-b)indole], aminomethyl-
α-Carboline (2-amino-3-methyl-9H-pyrido (2,8-bl indole), IQ (2-amino-8
-Methylimidazo[4,5-f]quinoline, 2-AAF
(2-acetylaminofluorene), ethidium bromide, MeIQX (272/-8,8-dimethylimidazo-[4,5-/)quinoxaline), 9-aminoacridine, quinacrine, 8-methoxypsoralen, chlorpromazine, Norharman (β -Carboline) In the present invention, animal fibers include +, tM hair, and silk. These may be commercially available refined wool or silk threads, but may also be ones that have been washed and purified using a solvent depending on the purpose.

In order to adsorb polycyclic organic substances onto animal fibers, for example, after adding the animal fibers to a solution containing the polycyclic organic substances, especially an aqueous solution, usually 0-10.

C1 It is preferably carried out by stirring, shaking, etc. at 15-80°C. This operation may be repeated. It can also be carried out by filling a column with animal fiber and passing a solution containing a polycyclic organic substance through it. It can also be carried out by contacting untreated animal fibers.

To desorb polycyclic organic substances adsorbed to animal fibers, use a neutral, weakly alkaline, or weakly acidic solvent such as methanol, methanol-ammonia aqueous solution, methanol-hydrochloric acid solution, etc. This can be carried out by stirring and shaking at the following temperatures. Also,
When a column is filled with animal fibers and a solution containing a polycyclic organic substance is passed through the column, the column can be eluted by passing through the above-mentioned solvent.

The solution containing the polycyclic organic substance recovered in this manner can be further concentrated to have an even higher concentration. It can also be isolated by distilling off the solvent.

According to the method of the present invention, polycyclic organic substances can be selectively adsorbed onto animal fR fibers. It is particularly effective for polycyclic organic substances having three or more rings. The method of the present invention is particularly useful for selective adsorption, desorption and concentration of polycyclic organic substances, such as mutagenic substances, that exist in trace amounts in the environment, foods, luxury goods, biological samples, etc. It can be widely used for qualitative, quantitative determination, or removal of primary substances. For example, it can be used to quantify mutagens in river water, remove mutagens from heaf extracts, quantify mutagens in urine, and remove mutagens from cigarette smoke.

Next, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited to these unless the gist thereof is exceeded.

Example 1 1) Purified white braided commercially available fine sewing thread No. 9 18.25' was mixed with 15% of ammonia water.
After shaking mechanically with a mixture of 0mlCm ammonia water (50ml) and water 100ml for 80 minutes at 46°C, it is sent from house to house. Furthermore, with 7J of ammonia (151,5i (mixture of 1.5ml of concentrated ammonia water and 150ml of water))
Shake mechanically for 15 minutes at 5°C, wash separately with water until neutral, and dry. Furthermore, at 25°C (room temperature), 150rnl of dimethyl sulfoxide was added twice, and 150ml of methanol-concentrated ammonia water (volume ratio 50:l) was added twice.
Wash twice with 150 ml of methanol and dry. A purified autophase system 18.0P is obtained.

2) Example of adsorption and adsorption of various mutagenic substances by purified white silk thread: The purified white silk thread (10"9/m/) obtained in 1) was added to an aqueous solution of mutagenic substance 2 x 10 to 10X1O mol/l. Then, shake mechanically for 30 minutes at room temperature (25°C).Then, remove the silk thread and add fresh purified white silk thread (1OQ/
~/rnl) and shake mechanically for 80 minutes at room temperature. The purified white wool was then removed, and the mutagenic substances remaining in the solution were determined from the ultraviolet absorption spectrum of the solution, and the adsorption rates shown in Table 2 were calculated.

In addition, the purified white wool to which the above-mentioned mutagenic substance has been adsorbed is wiped with a paper towel, further moistened with water, and then wiped.

The purified white wool that has adsorbed mutagenic substances in this way is mixed with 1 ml of methanol-concentrated ammonia water (50:l volume ratio)/
Lom9c purified white silk thread) and shaken for 15 minutes at room temperature to extract.

Take out the purified white silk thread, wipe it with a paper towel, and extract it again in the same way using methanol-concentrated ammonia 7J (lx). Mix the methanol-concentrated ammonia water extract solution twice and remove the mutagenic substances in it. The recovery rates in Table 2 are calculated from the ultraviolet absorption spectrum.

Table 2 Procedural amendment (spontaneous) 6゜May 8, 1980 1, Indication of the case 1982 Application for permission No. /': I/da 3 No. 2, Name of the invention Process for treating polycyclic organic substances 3. Relationship with the case of the person making the amendment Patent applicant address 5-chome Kitahama, Higashi-ku, Osaka ■ Street name (209) Sumitomo Chemical Co., Ltd. Representative Takeshi Hijikata 4 Agent address 5-1 Kitahama, Higashi-ku, Osaka Contents of the Akiji amendment (1) In the specification column 8, lines 14-15, [Animal fibers include wool and silk in particular.

These include "Animal fibers include wool, wool,
Silk and hair can be given. Wool and silk are 3゜
.

(2) Delete 8 lines from the bottom of page 8, "Hair can also be used."

(8) Page 6, line 15, page 7, line 16, page 7, lines 2-8
Line 4, Line 6, Line 9, Lines 16-17, Page 8, Table 1, Line 3 from the bottom of Page 8, Lines F to 2
Line, page 9, line 3, page 6, line 9, page 1 e=
t In line 7 and Table 2 on page 10, the words "mutagenic substances" are corrected to "polycyclic organic substances."

that's all

Claims (1)

[Claims] A method for treating polycyclic organic substances, which comprises adsorbing polycyclic organic substances using animal fibers and desorbing them as necessary.