JPS6016579A - Tasty drink blend containing agent for counter acting mutagenesis - Google Patents

Abstract

PURPOSE:The titled blend having raised safety and improved taste, obtained by blending an agent for counteracting mutagenesis comprising a pharmaceutical preparation of a germ consisting of malt, soybean, rice bran or an extracted fraction of them with a tasty drink component. CONSTITUTION:For example, coffee such as coffee bean, or ground coffee bean is blended with 0.25-2.5mg (usually based on coffee concentration for drinking) malt powder, soybean powder or rice bran, or 0.05-0.5mg powder of extracted fraction of malt, soybean or rice bran. Consequently, about 50-100% mutagenesis caused by Salmonella typhimurium TA 100 strain of coffee will go out.

Description

DETAILED DESCRIPTION OF THE INVENTION (5) Technical Field The present invention relates to a combination of an antimutagenic agent and a beverage ingredient, which is a germ preparation consisting of malt, soybean, rice bran, and their respective extract fractions.

(B) Background Art Currently, most human cancers are thought to be caused by carcinogenic factors in the living environment. It is estimated that only about 2% of human cancers are caused by genetic factors alone (1-11gg1nson).

J., & Muir, C.S.: J., Natl., Ca'n.
ccr'Jnst; Vbl, 163.1291.
'79).

The food and drinks we consume on a daily basis are considered the most important cause of cancer, which accounts for approximately /~/ of human deaths, and there is an overwhelming amount of epidemiological data that supports this view. .

In recent years, the Salmonella test (also known as the Ames method), which uses histidine requirement as an indicator, has been established as a means of predicting the carcinogenicity of environmental factors such as foods, drinks, and pharmaceuticals.
Ames+I1. N. et al.: Mutatio
nRes,, Vol, 61,347. '75). Many research institutions have found a high correlation of 80-90% between the mutagenicity and carcinogenicity of compounds tested by water washing (McCann+J, et al: P,
rocNatl, Acad, Sci, (U, S,
A.), Vol. 72, 5135.

'75).

Therefore, eliminating mutagenicity is expected to have a considerable effect on protecting humans from cancer-causing risks. Furthermore, mutations occur as a result of damage to chromosomes or DNA, and even if they do not lead to cancer, the influence of mutagens on human health cannot be underestimated.

This is why food and drink products that are not mutagenic are desired.

Today, mutagens that are clearly found in foods, drinks, and luxury goods include benzo[a]pyrene and aflatoxin B.
1.2-Aminofluorene, benzCa) Anthracene, chrysene, dimethylnitronamine, β-naphthylamine, nitrosopyrrolidine, methylglyoxal, and many other substances that have been proven to be carcinogenic. but,
No matter how much mutagenicity is recognized, it is a hot topic to take legal prohibition measures in the case of products that have been closely connected to human life, such as food, drinks, and luxury goods. It is also difficult to live without it.

In particular, many luxury foods have higher mutagenicity than everyday foods and drinks (see Table 1).

Table 1 Mutation activity of luxury items Luxury items Number of reversion mutation colonies 1 cigarette 5.5 [] 0 (TA98.+
"") Instant coffee 1F (150ml)
45. O[]0 (TAloo, -) Regular: J-
44-1 cup (150+++/) 195.000 (T,
A10[],-) 1 cup of black tea (150p+/) 68.0
00 (TAL 00.-) 1 cup of green tea (i 5 Q m
l) 21,000 (TAIDO,) 1 cup of Sencha (1
5071J) 17000 (TAloo, -) Ended Salmonella Typhimurium (Salnlonellat)
yphimurium) TA 100 stocks or 1
°A98 strain was used.

The presence or absence of raw rat liver homogenate (S9 mix) is not shown.

(+): S9 mix added, f-j: 89 mix not added.

In fact, tobacco and oropharyngeal cancer, 1st case head cancer, esophageal cancer, 1j caries for stomach hypnosis, coffee and 14-year-old masturbation, 11)
It has often been reported that there is a relationship between alcoholic beverages and esophageal cancer, liver cancer, etc.

The present invention aims to eliminate or attenuate the mutagenicity of luxury goods, thereby improving the safety of the goods and, ultimately, restoring the reliability of the goods.

Coffee is said to have been placed in everyday life as a daily drink in the 1400s in Gamma Lapia. Since then, as human society has become more highly civilized, the demand for it as a luxury item has increased more and more. Japan has also recently recorded rapid growth in coffee consumption (approximately six times in the ten years since 1969).

However, in recent years, concerns have begun to be raised about coffee's negative effects on health in various ways, and it is believed to be a major reason why coffee consumption in the United States slumped last year. What about caffeine? ! The relationship between 11-cell toxicity and other cancers, especially the correlation between coffee drinking and pancreatic cancer pointed out by Dr. MacMahon, caused a sensation mainly in Europe and America (MacMahon+B).

et al.: N. Engl. J. Med., Vol.
, Ro04,6 Ro0゜1981). Other than pancreatic cancer, the other organs where coffee increases cancer incidence are the lower urinary organs, including the bladder. (Sjmon, D, Ct
2t,: J, Na1l.

Cancer In5t, Vol, 54+
587 + 1975). Furthermore, prostate cancer, leukemia,
There are also reports that it increases the incidence of ovarian cancer, and more detailed research is awaited in the future.

Regarding the mutagenicity mentioned above, coffee has a high level among everyday foods and drinks (see Table 1).

Nowadays, when coffee is consumed in large quantities as a daily drink, there is an urgent need to eliminate the various points that are considered harmful to health, and research in this field has produced many results to date. For example, the carcinogen Trp-P-
Mutagenic inactivation effects of carcinogens have been reported for peroxidase against 1, thiol compounds against N-medyruN'~nitrosoguanidine 1, and selenium compounds against A12-dimethylbenzanthrahin, respectively. (Martin, s, E,
et al.: MutatinnRes., Vol.
, 82.41. 1981). However, many inactivating agents are themselves harmful to living organisms or are unsuitable for regular consumption due to palatability, making it difficult to put them into practical use.

In order to protect human health, it is absolutely necessary to eliminate the cytotoxicity and mutagenicity of coffee with substances that are harmless to humans and do not affect the flavor, aroma, or other characteristics of coffee drinking. It will be needed.

(C1 Disclosure of the Invention In order to achieve the above-mentioned object, the present inventors have made extensive research efforts and hereby presently present malt, soybean, rice bran, and their respective extract fractions (hereinafter collectively referred to as the "Pacific Germ Preparation"). We have discovered the fact that coffee has remarkable efficacy as an inactivator for mutagenicity7.The above ingredients can be used to create non-mutagenic beverages or to eliminate the mutagenicity of beverages. However, the use of the above ingredients is completely new.

The amount of germ preparation required to suppress the mutagenicity of coffee is the same as that of malt flour, soybean flour, or rice bran, relative to the coffee concentration normally consumed by humans (as coffee powder, 5 II 9-20 evening/rtrl). 0.25 to 2.5 m, barley: 1.05 to 0.5 m as a powder of soybean and rice bran extract fraction (hereinafter referred to as germ extract powder), and the following effects can be achieved with this added amount. It is possible to obtain

However, when drinking coffee with hot coffee, a certain degree of decrease in activity may be avoided.
It may be sufficient to use an amount 2 to 6 times the amount added above.

Salmonella typhimurium (from coffee) than K.
5abnonella typhimurium)Ti
The mutagenicity against the 2O3 strain is approximately 50% to 100%.
%Disappear.

□Coffee can be made by mixing coffee beans or ground coffee beans with germ preparations (regular type), or by adding germ preparations to coffee liquid extracted from roasted coffee beans (
o, coffee type (e.g. canned coffee), or by homogeneously mixing the germ preparation with coffee powdered by spray drying or freeze drying, or by spraying the four rules as a liquid onto coffee powder (fluidized bed granulation). (instant coffee type).

Coffee whose mutagenicity has been reduced or completely inactivated by washing with water has excellent palatability, and is therefore considered more desirable for the purpose of reducing risks to human health.

In addition to coffee, the germ preparation also inactivates the mutagenic effects of tea and straight bourbon.

As for black tea, mutagenicity can be reduced by mixing the germ preparation into powder after drying with heat or by attaching it to the tea leaves using fluidized bed granulation.

1. May be mixed in powder or solution form when drinking. Even with straight bourbon, its mutagenicity can be inactivated by incorporating the germ preparation into the product itself or by adding it in the form of powder or solution when drinking.
c-Kill. Market filli value of product appearance, etc. 1
It is preferable to use malt, soybean and rice bran extract fractions in order to avoid lag.

The present invention is based on the above novel findings.

■) Example ■) Method for measuring the mutagenicity original and mutagenicity suppressing effect (i) Method: Solle incubation m (cedar wood, long legs;
Chemical Mutagiens, Volume 6, Page 41, 1981
)by.

(11) Bacterial strain used: histidine auxotrophic Salmonella la typbi
murium) Ti2O3 strain (hereinafter referred to as "S.Ti1O0 strain", 1-t).

all) Sample preparation a) For instant coffee: Dissolve instant coffee (powder) in distilled water. On the other hand, a predetermined amount of the germ preparation is also suspended or dissolved in distilled water, and both solutions are mixed in an amount of 50111.

For regular coffee: Extract the roasted coffee beans with 250 d of hot distilled water per 20,000 ml of hot distilled water, and filter the extracted liquid with a coffee filter (Kalita Ita 12).
j pass. Freeze-dry the p solution, measure its dry weight, and then dissolve it in distilled water. On the other hand, a predetermined amount of the embryo preparation is also suspended or dissolved in distilled water, and 50 μl of both solutions are mixed.

C) Black tea 5-7I commercially available at 2001fLl
Extract with boiling distilled water. After extraction, the leaves are removed, the extract is freeze-dried, and distilled water is added to the dried product to dissolve it to a predetermined concentration. On the other hand, a predetermined amount of the germ preparation is suspended or dissolved in distilled water, and both solutions are mixed at a ratio of 1=4 to make a total of 100 μl.

2) For bourbon 2) Add commercially available bourbon (also known as straight bourbon or bourbon whiskey) to 40% under reduced pressure.
Concentrate to dryness using an evaporator at °C. D M'S
After OK and re-dissolving, suspend or dissolve a predetermined amount of the germ preparation in distilled water, mix equal amounts of both solutions, and make a total of 100
Let it be μl.

e) Germ preparations: Among the germ preparations, rice bran is used that peels off during rice milling, and malt flour and soybean flour are each ground into powder.

Malt, soybean, and rice bran were each used in the experiment by adding distilled water to a predetermined amount. In addition, each extracted fraction dried powder is 1g each.
was extracted with 10ml of distilled water at 4°C for 30 minutes, centrifuged the extract for 1'+1 (1,000xg), filtered the supernatant through a Millipore filter (pore size: 0.45μm), and filtered the filtrate. It is freeze-dried and powdered. A predetermined amount of this was washed with distilled water, dissolved, and used for experiments.

+v) Measurement of mutagenicity front), each sample obtained by (a) to) 10'0μ
500 μl of 0.1 M sodium phosphate buffer (
pH7,4)K100/11! I) S, TAL
00 strain culture solution is added. Add this mixture to 5
After shaking for 20 minutes at 7°C, mix with 2 ml of dissolved soft agar and spread on 1% glucose agar plates. In addition,
0.1 micromol/2 ml soft agar/plate of histidine, which is necessary to divide the bacteria several times on the plate, is added to the soft agar. After standing at 67℃ for 48 hours,
Count the number of colonies on the plate as revertants. In addition, the mutagenicity suppression rate is calculated using the following formula.

×100% ■) Results (1) Mutagenicity suppressing effect of germ preparation on coffee.

(a) Regular coffee Dissolve 15 mg of coffee bean extract powder prepared in the above-mentioned method in distilled water to prepare 5D#. On the other hand, the embryo preparation is suspended or dissolved in 50 μl of VC in a predetermined amount of distilled water, the two are mixed, and the mixture is incubated at room temperature for 20 minutes. This was added with 0.1M sodium phosphate buffer (pH 7.4).
) 0.5 ml and 0.5 ml of the culture solution of the S and TA100 strains.
I Wtl was sequentially mixed and mutagenicity was measured by the method described above in tc)B(+), and the results shown in FIG. 1 were obtained.

The mutagenicity of regular coffee is 5% <-w/w> of the extracted coffee powder.
Add soybean flour, rice bran, or 1.0% (w7w)
Most of it can be inactivated by adding dry powder extracted from malt, soybeans, and rice bran. For complete inactivation of mutagenicity, it is necessary to add 20% of malt flour, soybean flour, and rice bran to the coffee powder, and 4% of malt, soybean, and rice bran extract dry powder.

(b) Instant coffee Using commercially available instant coffee powder, the antimutagenic activity of the germ agent was examined in the same manner as for regular coffee. The results are shown in Figure 2.

In the case of instant coffee, it is the same as regular coffee mentioned above (mutagenicity is significantly reduced or completely eliminated by the germ preparation. That is, 5% malt flour, soybean flour, rice bran is added to the instant coffee powder)
(~■/~■), or by adding dried flour of malt, soybean, and rice bran extract fractions (W/ko), most of the mutagenicity can be inactivated. Mutagenicity can be completely eliminated if the amount added to coffee powder is 20% as malt flour or soybean flour, and 4% as dry powder of sprouts and soybean extract fraction. In the case of nirata and rice bran,
They are 50% and 6%, respectively.

Both regular coffee and instant coffee are S9 mixes (1 old
- The mutagenicity that had been inactivated by the addition of the adenine dinucleotide phosphate (NADPII) production system did not reappear.

(11) Suppressive effect on the mutagenicity of germ preparation black tea.

The black tea prepared in the above-mentioned manner is as shown in Figure 6r (as shown).
It has mutagenicity against Salmonella enterica (S, TAIDO) K at a frequency of approximately 200/10 viable bacteria per 1 egg. On the other hand, the mutagenicity of malt, soybean, and rice bran is significantly increased by adding 20% or dry powder of malt, soybean, and rice aft extract fractions to a concentration of 4%. decreased to

(Inhibitory effect of liD embryo preparation on the mutagenicity of straight perphone.

Straight bourbon, as shown in Figure 4, has an 8.
TAL 001/j versus fl has mutagenicity (approximately 800 revertant colonies/108 viable populations). This is V
(20% as malt flour, soybean flour, rice bran (W/W)
) or each extracted fraction as dry powder at 4% (W/W
), its mutagenicity is approximately 40
~85% were inactivated.

(Beyond industrial applicability, mutagenicity has been completely reduced and eliminated by germ preparations, and the original taste and aroma of coffee'f and other beverages are impaired.) In addition, since the germ preparation itself contains vitamin C 1'ff: Toyohata (/C), it can be commercialized as a tights drink that is more desirable for human health.

[Brief explanation of the drawing]

Figure 1 is a graph showing the suppressive effect of germ preparations on the mutagenicity of Salmonella deifimurium '1'A10U strain in regular coffee; Figure a) shows malt flour and its extracted fraction dried powder; , b) Figure shows soybean flour and its extracted fraction dried powder, and C) Figure shows rice bran and its extracted fraction dried powder. In Figure 1, ・ indicates additive-free coffee, M, M, Oka indicate coffee with 20% (~l/W) malt flour, soybean flour, and rice bran added. 1, △,, 1] 4%
(W/W) coffee containing malt, soybean, and rice bran extract fraction dried powder. Figure 2 is a graph showing the inhibitory effect of germ preparations on the mutagenicity of Salmonella typhimurium TA100 strain in instant coffee. and soybean extract fraction dry powder, C) Figure is a graph about rice bran and rice bran extract fraction dry powder. The meanings of 0, ^, ma, △, , bar and mouth in Figure 2 are as follows.
Same as the figure. FIG. 3 is a graph showing the reduction of black tea mutagenicity against Salmonella typhimurium'rA100 strain by the germ preparation. In Figure 6, 0 indicates black tea without the addition of germ preparations, and Mu, Ma, and Bar are malt, soybean flour, and rice bran totaling 20% (W/
W), the mouth is malt, soybean, rice bran extract fraction dry powder f
:4%l/W) added black tea. Figure 4 shows 'Salmonella deifimurium TA100.
Figure 3 is a graph showing the reduction of bourbon mutagenicity by germ formulation on strains. The middle part of Figure 4 is bourbon with no germ preparation added, and Mu, Ma, and Akebono are malt flour, soybean flour, and rice bran f.
: 2L 1% (W/W), △, Mouth indicates bourbon with malt, soybean, and rice bran extract fractions and dried gold powder 44 (W/W) added. (4 others) Procedural amendment 1982 l) Month C', > t - Japan Patent Office Technical Officer Aio Wakasugi 1, Indication of the case 1982 Patent Application No. 12424 ri No. 2, Name of the invention Antimutagen Caller's Beverage Formula 13 Containing Sex Agents, Relationship with the Amendment Case Patent Applicant's Address Name (190) Suntory Ltd. 4, Column 6 of [Detailed Description of the Invention] of Attorney's Specification, Xun (i positive internal) For details as shown below θ) <aJ' i to i” minus line Before correction After correction 13 6 ?il (C) 1 f: rtr (D
116 15 V, A A, %f f, + -) - 384-

Claims (3)

[Claims] (1) A combination of an antimutagenic agent comprising a germ preparation selected from the group consisting of malt, soybean, rice bran and their respective extract fractions and a beverage ingredient. 2. The formulation of claim 1, wherein the beverage ingredient is a coffee ingredient selected from the group consisting of coffee beans, coffee bean powder, coffee bean extract, and dry powder of coffee bean extract. (3) The composition according to claim 1, wherein the beverage ingredient is black tea.