JPS60120250A - Semi-quantitative analysis of chemical component-containing solution - Google Patents

Abstract

PURPOSE:To make it possible to simply and rapidly perform the titled analysis at an arbitrary place even by an operator other than an experienced expert, by using a color standard, which is obtained by superposing a chemical component-containing solution having no reagent mixed therein to a color standard, as a standard. CONSTITUTION:Gelatin solutions containing coloring matter in various concns. are applied to a colorless transparent cellulose acetate film and a color standard table 3, to which coloring layers corresponding to concns. of a specific chemical component, for example, 0-5ppm, is held between a rear surface plate 1 comprising a mild white acrylic plate capable of scattering light and a front surface plate 2 comprising a colorless transparent acrylic plate. Further, a water inspecting container 4 is erected in front of the color standard table 3 by a container stand 6 so as to erect a discolored water inspecting container on its side. In addition, transmitted light from a light source (not shown by the drawing) positioned behind the rear surface plate 1 is received in front of the containers 4, 5 and the colored concns. thereof are compared to visually judge the concn. of a specific chemical component wherein color densities of both transmitted light beams are equal.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a semi-quantitative method for chemical component-containing solutions, and more specifically, it relates to a semi-quantitative method for chemical component-containing solutions, and more specifically, it relates to solutions such as aqueous solutions used in various treatments, waste liquids, waste liquids, and other liquids. The present invention relates to a method for semi-quantifying the content of a specific chemical component contained in a chemical component-containing solution by comparing it with a color standard.

[Prior art]

Analysis of specific chemical components in aqueous solutions is an inevitable operation for controlling aqueous solutions.

However, many chemical analyzes require special equipment and complicated procedures. For this reason, there has been a desire to develop a quick and simple analytical method that can be used at any location where analysis is required and that does not require a chemical expert.

Various simple analysis kits are known to meet this desire.Among these simple analysis kits,
For those that utilize coloration or decolorization reactions, instead of the spectrophotometer normally used for this type of analysis, there is a semi-quantitative method by comparing with a color standard prepared in advance to correspond to the amount of chemical components. It has been adopted.

For example, with the Bonnard kit manufactured by Assimilation Research Institute, after collecting a certain amount of sample water in a test tube, one tablet as a coloring reagent is added, and the color development is determined by adding resin containing various concentrations of pigments. Semi-quantitative measurements are made by comparing the cylindrical shapes with color standards stacked in a mosaic.

However, although this method is effective when the sample water is nearly colorless and transparent, when the sample water is colored before the addition of the coloring reagent, comparative quantification becomes extremely difficult. In other words, if the sample water itself is colored, it is necessary to take that coloring into consideration and compare it with the color standard, but it is necessary to subtract the coloration of the sample water itself and compare it with the color standard, which causes subtle color changes. It is often impossible even for experts to make judgments by comparing with color standards.

For this reason, it is possible to prepare a color standard that takes into account the coloring of the sample water itself, but it is necessary to prepare a color standard that takes into account not only the difference in hue of the coloring, but also the saturation and lightness. This is difficult to implement in practice because the number of types is enormous.

[Purpose of the invention]

The purpose of the present invention is to determine the chemical component content by comparing it with a color standard, even if the test water (test sample) itself is colored, regardless of its hue, saturation, degree of BA, etc. The object of the present invention is to clarify a semi-quantitative method for chemical component-containing solutions that can be carried out easily and quickly at any location, even by non-experts.

Other objects of the invention will become apparent from the following description of the specification.

[Summary of the invention]

As a result of intensive research, the inventor of the present invention mixed a chemical component-containing solution with a reagent that quantitatively changes color by reacting with the chemical component, and compared it with a color standard to determine the chemical component content of the chemical component-containing solution. It has been found that the above object can be achieved by using, as a standard, a color standard obtained by superimposing a chemical component-containing solution that is not mixed with the reagent on the color standard, and the present invention It came to,
In a preferred embodiment of the present invention, a color standard prepared in advance is displayed in a planar shape, and the method of the present invention is carried out using the planar color standard.

[Structure of the invention]

The present invention will be described in further detail below.

The method of the present invention can be applied to semi-quantitative determination of all chemical component-containing solutions using quantitative color change reactions (e.g. coloring or decoloring reactions) of any substances or ions, such as metals, inorganic non-metals, organic substances or ions. I can do it.

In the present invention, the chemical component-containing solution without mixing reagents (hereinafter referred to as test water) used by superimposing it on the color standard is basically an aqueous solution that is not subjected to pretreatment.
It is not limited to this. Specifically, it is preferable to use the solution immediately before adding the reagents necessary to cause a quantitative color change, and also to add pnP! in advance to cause a quantitative color change reaction. If a treatment such as conditioning, pre-oxidation or pre-reduction is required, the liquid after such treatment is preferred.

In the present invention, the color standard on which the test water is superimposed (hereinafter referred to as color standard) refers to the color density obtained by reacting with a reagent that reacts with chemical components to quantitatively change color. First, it is a color chart prepared, for example, as a table, corresponding to the concentration of the chemical component. Various color markings have been known in the past, and these known color markings can be employed in the present invention, but it is preferable that the color markings in the present invention have translucency. That is, it is preferable to be able to compare and observe the color density in the total transmitted light of the transmitted light of the color standard alone and the transmitted light of the sample water. The direction of the transmitted light may be from the color standard front side toward the test water side when observing from the test water side (generally, this is preferable); on the other hand, when observing from the color standard front side, , in the opposite direction to the above.

The color marker coat that is particularly effectively used in the present invention is a plate-shaped molded product containing a pigment.Such color marker coats contain pigments of various concentrations and are molded in a flat plate shape. It may be made of a polymer resin or glass, or it may be a plate-shaped resin or glass on which a dye dissolved in a solvent is sprayed or applied. Alternatively, a film containing a pigment such as cellophane, a hydrophilic binder containing a pigment (gelatin, etc.), or a film coated with a hydrophilic binder (gelatin, etc.) containing a pigment may be placed on a glass or polymer resin plate. It may be an attached letter or a letter inserted between them.

In this case, when the color marker coat is inserted between two members, it is preferable that the member on the side of the color marker coat that overlaps with the sample water is transparent, and the member on the opposite side transmits light. It would be better to have an opaque or translucent white plate that allows light to pass through, or an opaque or translucent white plate that allows light to pass through, as it would make it harder to see the light source. Easy and preferable. Here, opaque or translucent white includes cases where light can be scattered by a rough surface such as ground glass.

In addition, when inserting a color-marked single coat between two pieces as described above, if the side that overlaps with the sample water is colored liquor, prepare a color-marked single coat that takes into account the coloring. Yes.

The color marker of the present invention does not need to be placed between two members as described above, and may be attached to a part (preferably uncolored) that can scatter light, or In addition to such a member, it may also be installed in a replaceable manner, such as a sliding type, to any support part.

In the present invention, the transmitted light that passes through the color marker coating and sample water and the chemical component-containing solution that has been mixed with a reagent to exhibit discoloration (
Hereinafter, this is referred to as discoloration water test. ), the conditions for the outside observer's vision, especially the light transmission conditions (thickness, width, shape of the container, etc.) and the conditions that cause coloring, must be the same6. The conditions that cause coloring must not be such that a coloring component is present only in the middle of the path of one of the passing lights.

However, this does not apply if the color standard is created taking into account the presence of the coloring component. This also applies to containers for water samples and discolored samples.

The containers containing the test water and the discolored test water have the same conditions that cause coloration when exposed to transmitted light.
Preferably, it is a polymer resin.

Here, the colorless and transparent container is one made of glass or polymer resin, and its shape does not matter. For example, even if a cylindrical container such as a test tube is placed over a flat plate-shaped color marker coating, the lens effect will create an effect that makes it appear as if the liquid is actually colored to the color of the color marker coating. The color water test of the present invention is preferable because it can obtain a color standard, and even if it is a square cell-shaped container, a good unique color standard can be obtained in combination with a flat color standard coat. It is obtained by adding a sample (hereinafter referred to as a coloring reagent) that quantitatively changes color by reacting with a specific chemical component in the sample water to the sample water, mixing it, and completing the color change reaction. . The color change reaction time varies depending on the type and concentration of the chemical component to be tested, the seed portion of the coloring reagent, 0 degrees Celsius, and reaction conditions such as temperature and stirring.

The coloring reagent used in the present invention may be any conventionally known coloring reagent and may be selected depending on the chemical component to be tested and the color target. The semi-quantitative method in the present invention is performed by visual judgment. That's enough. Visual observation may be performed by direct or indirect methods using the transmitted light of the sample water and the single color marker coat and the transmitted light of the discolored test water. As described above, the color density due to the overlapping of the sample water and the single color label coat,
Regarding the comparative observation with the color density of the color test water, the case where transmitted light is used has been described, but the method is not limited to this, and reflected light may also be used. That is, the rear plate of the single color marker coat may be opaque.

An example of an apparatus preferably used in the method of the present invention is shown in FIG. 1, and this specific example will now be described.

In the figure, 1 is a rear plate made of a milky-white acrylic plate that can scatter light, 2 is a front plate made of a colorless and transparent acrylic plate, and 3 is a vertically arranged color marking plate made of colorless and transparent cellulose. Gelatin containing various concentrations of pigments (for example, according to Food Red No. 102) is coated on an acetate film base, and the concentrations of specific chemical components in the sample water are [1, 0, 5, l, 2, 3, Products having colored layers with concentrations corresponding to 4 and 5 ppm are sequentially arranged in parallel, and
It is inserted between the rear panels. 4 is a water test container, and 5 is a discolored water test container, each of which can be placed upright in a container mold 6. At this time, the water test container 4 is placed in front of the color marker single coat 3. Also, the discolored water test container 5 is
It is arranged so that it does not overlap with color standard clothing 3.

To carry out the method of the present invention using this device, a light source (white light is preferred) is used. ] facing the rear of the rear panel 1, and place the water test container 4
and the front side of the color-changing water test container 5), receive each bursting light from the light source, compare the degree of coloring density, and visually determine the specific chemical component concentration (ppm) where the coloring density of both transmitted lights is the same. do it.

〔Example〕

Hereinafter, the present invention will be explained in more detail with reference to Examples.
The embodiments of the present invention are not limited to these.

Wastewater was collected from three chemical factories A, B, and C - A was pale yellow in color, B was pale purple, and C was pale green in color. After adding sodium L-ascorbate lo9 to approximately 10 td of each of these three types of waste liquid, prepare two test tubes for each type, and add K 2 mJ to one side and the remaining liquid to the other. I put it close to my mouth. To the former, add 2 drops (approximately 0.08 m) of a pre-adjusted coloring reagent (liquid) of 2 g of pennyl alcohol, 3 g of ethylene glycol, 1 g of α, α'-dipyridyl (liquid) using a dropper, and apply it with your fingertips. After capping it with a bottle and shaking it for about 2 seconds to develop the color, insert it into the right side of the container chamber 6 of the color standard device shown in Figure 1. 6 and compared, the iron ion concentration was determined.The results are shown in Table 1 along with the analysis values by atomic absorption.

As is clear from Table 1 M1, the test solution is colored before adding the coloring reagent, so it could not be quantified by the conventional method of directly comparing it with the color standard, but the analytical method of the present invention It turns out that it can be well quantified.

[Brief explanation of drawings]

Figure 1 shows the color standard device used in the example.
It has a structure that also serves as a container (test tube) stand. In the figure, 1 is a rear plate, 2 is a front plate, 3 is a color standard coat, 4 is a water test container, 5 is a discolored water test container, and 6 is a container chamber.

Claims (1)

[Claims] A method for semi-quantitatively determining the chemical component content of a chemical component-containing solution by mixing a chemical component-containing solution with a reagent that quantitatively changes color by reacting with a chemical component, and comparing the reagent with a color standard. A semi-quantitative method for a chemical component-containing solution, characterized in that a color standard obtained by superimposing a chemical component-containing solution on the color standard is used as a standard.