CN218823895U - Reagent subpackaging colorimetric tube for water quality rapid analysis - Google Patents

Abstract

The utility model discloses a reagent partial shipment colour comparison tube for among quality of water rapid analysis, include: a transparent square tube body with an opening at one end for loading reagents; the square pipe cover is matched with the opening of the square pipe body and used for blocking the opening; the wall thickness of the square pipe body is 1mm, the length and the width of the inside of the square pipe body are both 1cm, and the length and the width of the outside of the square pipe body are both 1.2cm. The reagent subpackaging colorimetric tube of the utility model has the functions of the reaction tank and the detection tank at the same time; meanwhile, the length and width of the colorimetric tube are completely matched with all ultraviolet-visible spectrophotometers and all fluorescence photometers on the market, the colorimetric tube after reaction can be directly placed in an instrument for reading, and the quantitative analysis of a photometric detection method can be completed without changing the structure of a conventional instrument; and during color comparison, the color observed by the square tube is closer to the real color.

Description

Reagent subpackaging colorimetric tube for water quality rapid analysis

Technical Field

The utility model relates to a can realize reagent partial shipment colour comparison tube of visual colorimetry and photometric detection method simultaneously belongs to analytical chemistry and quick quantitative technical field.

Background

Visible spectrophotometry (also called as "colorimetry") is a detection means which is most widely researched and applied in the field of chemical analysis, particularly in the field of water quality detection, due to the characteristics of cheap used instruments, various related chemical reactions, good selectivity, detection sensitivity enough to satisfy constant analysis, mild reaction conditions, good stability of color development products, simplicity in operation and the like. In the basic principle of realizing visible spectrophotometry detection, for an incremental method, a colorless object to be detected is usually mixed with a specific certain (or several) same colorless reaction reagent, and then a chemical reaction is carried out to generate a colored product, and under the condition that all reagents in a reaction system are excessive, the content of the object to be detected and the color depth or absorbance of the generated colored product are in a positive correlation relationship; in the subtraction method, a colorless analyte and a specific certain color reagent are subjected to a chemical reaction, and the content of the analyte is in a negative correlation with the residual color shade or absorbance of the color reagent. In the above chemical reaction process, the analyte can be semi-quantitatively and quantitatively analyzed by directly observing the color (i.e., visual colorimetry) and absorbance (i.e., photometric detection) of the product or the reagent consumed. In addition, for some chemical reactions which do not show color (participation of organic matters, particularly organic matters with rigid plane structures), the quantification of the substance to be detected can also be realized through fluorescence increment or fluorescence quenching in the reaction system.

In the practical application based on the principle of the method, firstly, standard solutions of objects to be detected with the same volume and different concentrations are respectively added into a series of test tubes, then all reagents are added, chemical reaction is carried out under the same conditions, after the reaction reaches equilibrium (namely products are not increased), part of the reaction solution is moved into a cuvette, and finally, a visible spectrophotometer or a fluorescence spectrophotometer is used for detecting to obtain the absorbance or fluorescence intensity of the reaction mixed solution in the cuvette, so that a standard curve of the objects to be detected is established; for the determination of unknown samples, under the same conditions, an actual water sample is used to replace a standard solution to participate in the reaction, and the absorbance or fluorescence intensity of the actual water sample is used to calculate the corresponding concentration after the same process is carried out. It can be seen that, although the flow is simple, the specific steps of preparation, addition, reaction, pipetting and the like of the reaction reagent still have high requirements on the expertise and proficiency of the operator, so that good precision and accuracy can be obtained, which is not favorable for popularization of the method and cannot meet the use scene requirements of outdoor simple and crude field rapid analysis.

SUMMERY OF THE UTILITY MODEL

In order to simplify above-mentioned operation process, reduce operating personnel's professional requirement and reach "one-step method" as far as possible and operate the purpose of accomplishing the detection, the utility model provides a can realize the reagent partial shipment colour comparison tube scheme of visual colorimetry and photometric detection method simultaneously. In this scheme, unite two into one the chemical reaction emergence container with the measuring vessel, through having preassembled different kinds of solid powder reagent and glyptic water sample scale mark in the colour comparison tube, when using, only need with the water sample add the colour comparison tube in to the scale mark can. After a fixed reaction time, comparing the color of the colorimetric tube with a matched standard gradient color card to complete semi-quantitative analysis of a visual colorimetric method; meanwhile, the colorimetric tube can be directly placed in a conventional visible spectrophotometer or a conventional fluorescence photometer for reading, so that the quantitative analysis of the photometric detection method is completed.

The utility model provides a scheme as follows:

a reagent partial shipment colour comparison tube for in quick analysis of quality of water includes: a transparent square tube body with an opening at one end for loading reagents; the square pipe cover is matched with the opening of the square pipe body and used for blocking the opening; the wall thickness of the square pipe body is 1mm, the length and the width of the inside of the square pipe body are both 1cm, and the length and the width of the outside of the square pipe body are both 1.2cm.

Further, the height of square pipe shaft is at least 5cm.

Furthermore, water sample scale marks are carved on the pipe wall of the square pipe body.

Furthermore, water quality detection parameters are engraved above the water sample scale marks.

Furthermore, four edges in the height direction of the square pipe body are subjected to fillet treatment.

Furthermore, the square pipe body is made of transparent high polymer materials.

Furthermore, the square tube body is made of polystyrene, ethylene terephthalate or methacrylate.

Furthermore, the square tube cover is an elastic rubber plug made of silica gel or butyl rubber.

Further, the square tube cover comprises a cover plug and a cover cap, and the surface of the cover plug is provided with threads; after the cover plug is plugged into the opening, the threads are tightly matched with the inner wall of the pipe body, and the cover cap is exposed out of the opening.

Further, the tube is pre-filled with solid powders of reagents for different water quality parameters.

The reagent subpackaging colorimetric tube provided by the utility model has the tube body part completely consistent with the length and width of a conventional cuvette (glass or quartz), the height of the tube body part can be flexibly prepared according to the water sample amount, and the tube body part can be directly adapted to all visible spectrophotometry and fluorescence photometers on the market without changing the instruments; meanwhile, the device has the functions of a reaction tank and a detection tank, and does not need to perform secondary liquid transfer after reaction; the solution color after reaction is observed through the plane of the colorimetric tube body, has stronger resolution than a circular test tube curved surface, eliminates the light condensation effect of the curved surface due to the observation angle and the distance, and is more real in observation.

In the further technical proposal of the utility model, the use cost is greatly reduced by adopting transparent high molecular materials (polystyrene, methacrylate, etc.); the reagent is preassembled in batches in the colorimetric tubes through a specific production jig, so that the reagent is better in consistency and higher in precision, and errors caused by artificial liquid adding in the chemical analysis process are avoided; the tube cover and the tube body of the colorimetric tube have good air tightness, and the stability and the quality guarantee period of the pre-packaged reagent can be improved.

Drawings

Fig. 1 is the reagent partial shipment colour comparison tube structure sketch map of the embodiment of the utility model.

Fig. 2 is a square tube body schematic diagram of the reagent subpackaging colorimetric tube of the embodiment of the present invention.

Fig. 3 is a schematic view of a square tube cover of a reagent subpackaging cuvette according to an embodiment of the present invention.

Fig. 4 is a front view of the square tube cover shown in fig. 3.

Fig. 5 is a use example diagram of the reagent subpackaging cuvette according to the embodiment of the present invention.

Fig. 6 is an operation flow diagram for measuring phosphate in water quality parameters by using the reagent subpackaging colorimetric tube of the embodiment of the present invention.

Fig. 7 is a phosphate standard curve obtained by dispensing the reagent into a cuvette according to the present embodiment through the operation flow of fig. 6.

Detailed Description

The invention will be further described with reference to the accompanying drawings and preferred embodiments.

The embodiment of the utility model provides a can realize the reagent partial shipment colour comparison tube of visual colorimetry and photometric detection method simultaneously in the quick analysis of quality of water use, refer to fig. 1 to fig. 4, this colour comparison tube mainly comprises two major part structures, square pipe shaft 10 and square tube cap 20 promptly. The square tube body 10 is transparent and has an opening 11 at one end for loading reagents. The square tube cover 20 is adapted to the opening 11 of the square tube body 10, and is used for blocking the opening 11, i.e. used as a cover for the opening 11. The wall thickness of the square pipe body 10 is 1mm, and the internal dimension is: the length and the width are both 1cm; the external dimensions are: the length and width are both 1.2cm. The size design is consistent with that of a conventional cuvette, so that the sample holder can be adapted to all ultraviolet-visible spectrophotometers and all fluorescence photometers when being used as a reaction pool and a detection pool. The height of the colorimetric cylinder can be determined according to the water sample dosage in practical application, preferably more than 5cm. Fillet processing is done to four edges on the direction of height of square pipe body, avoids pressing against the hand when handheld.

Referring to fig. 5, a water sample scale mark 12 is engraved on the wall of the colorimetric tube, and is generally 4 mL; and corresponding water quality detection parameters 13 are engraved above the water sample scale marks 12. The material of the square tube body of the colorimetric tube is a transparent polymer material, preferably Polystyrene (PS), polyethylene terephthalate (PET), methacrylate (PMMA), or the like, which has no absorption in the visible range and the ultraviolet-visible range, respectively. The square tube cover 20 of the colorimetric tube is an elastic rubber plug with certain hardness, preferably made of silica gel, butyl rubber and the like. Referring to fig. 3 to 5, the square tube cap 20 includes a cap 21 and a cap plug 22, and the surface of the cap plug 22 is threaded; after the cover plug 22 is plugged into the opening 11 of the square pipe body 10, the screw thread on the surface of the cover plug is tightly matched with the inner wall of the pipe body, and the cover cap 21 is exposed out of the opening to cover the outside of the opening. So that the colorimetric cylinder has good air tightness.

In addition, solid powder containing corresponding reaction reagents is pre-packaged in the colorimetric tube according to different water quality parameters; if the chemical reaction system with some water quality indexes relates to a liquid reagent which cannot be made into solid powder, the liquid reagent is held by a dropping bottle and a small amount of drops are dropped when the chemical reaction system is used. When the colorimetric tube is used, a water sample is added into the colorimetric tube to the scale mark of the water sample (whether liquid reagent is dripped or not is selected according to different reaction systems), and after a certain reaction time, the colorimetric tube displays a color with a certain shade degree. The color comparison tube is matched with a color comparison tube, and the color comparison tube is used for comparing the color of the color comparison tube after reaction with the color comparison tube, so that semi-quantitative reading of the visual colorimetry can be finished. The color chart is prepared by sequentially adding a series of standard solutions of objects to be measured with different concentrations into a colorimetric tube corresponding to water quality parameters, and after a fixed reaction time, according to the color of the colorimetric tube at the moment.

In order to further realize quantitative analysis, a series of colored colorimetric tubes obtained by using a standard solution of an object to be detected can be read in a conventional visible spectrophotometer to obtain a linear relation between the concentration and the absorbance of the standard solution, namely a standard curve. And substituting the absorbance obtained by the colorimetric tube added with the solution of the object to be detected into a linear equation of the standard curve for calculation to obtain the concentration of the object to be detected. For reaction systems with fluorescence intensity without color generation, quantitative analysis can be done with a fluorometer under the same operation.

Taking the detection of phosphate in the water quality parameters as an example, using the colorimetric tube provided in the foregoing embodiment, referring to fig. 6, a specific operation flow is as follows: (1) The utility model discloses in the colour comparison tube of embodiment pre-load phosphate participate in the solid reagent powder in the colour reaction (this colour comparison tube is called phosphate colour comparison tube this moment), including ascorbic acid, antimony potassium tartrate, filler; (2) Adding a phosphate standard solution or a water sample into the colorimetric tube to the scale mark of the water sample; (3) Dripping 2 drops of liquid reagent in a dripping bottle, wherein the liquid reagent contains ammonium molybdate and low-concentration strong acid solution; (4) Shaking to dissolve and uniformly mix the solid reagent powder, and standing for reaction for 1 minute; (5) And comparing the developed colorimetric tube with a standard gradient color chart for reading, or reading the absorbance by using a visible spectrophotometer.

Adding a series of phosphate standard solutions with different concentrations into a phosphate colorimetric tube, developing according to the process, detecting the absorbance at 700nm, and drawing to obtain a standard curve shown in figure 7.

The embodiment of the utility model provides an aforementioned reagent partial shipment colour comparison tube embodies following advantage with current scheme: (1) The colorimetric tube of this embodiment has the same length, width and wall thickness as the cuvette used with the uv-visible spectrophotometer and the fluorometer at present, and has designed a matched square cover, and can be preloaded with reagent, therefore has the functions of reaction tank and detection tank simultaneously, and the water sample does not need the secondary to move liquid to the cuvette after reacting in the tube and can detect. (2) At present, colorimetric tubes pre-filled with detection reagents are all round tubes, and on one hand, the colorimetric tubes cannot be directly adapted to an ultraviolet-visible spectrophotometer and a fluorescence spectrophotometer, namely, photometric detection cannot be directly carried out; on the other hand when observing the colour of colour comparison tube under the colour comparison method of visualing, the bellied surface (curved surface) of pipe can demonstrate the spotlight phenomenon similar to the magnifying glass, also can influence the differentiation to the colour exactly that the distance of observing, and the utility model discloses a colour is easily differentiateed to the plane of square colour comparison tube.

The foregoing is a more detailed description of the present invention, taken in conjunction with the specific preferred embodiments thereof, and it is not intended that the invention be limited to the specific embodiments shown and described. To the technical field of the utility model belongs to the prerequisite of not deviating from the utility model discloses, can also make a plurality of equal substitution or obvious variants, performance or usage are the same moreover, all should regard as belonging to the utility model's scope of protection.

Claims (10)

1. The utility model provides a reagent partial shipment colour comparison tube for among quick analysis of quality of water which characterized in that includes:

a transparent square tube body (10) with an opening (11) at one end for loading reagents; and

the square pipe cover (20) is matched with the opening (11) of the square pipe body and used for blocking the opening;

the wall thickness of the square pipe body (10) is 1mm, the length and the width of the inside are both 1cm, and the length and the width of the outside are both 1.2cm.

2. The reagent dispensing cuvette of claim 1, wherein: the height of the square pipe body is at least 5cm.

3. The reagent dispensing cuvette of claim 1, wherein: and water sample scale marks are carved on the pipe wall of the square pipe body.

4. The reagent dispensing cuvette of claim 3, wherein: and water quality detection parameters are carved above the water sample scale lines.

5. The reagent dispensing cuvette of claim 1, wherein: and four edges in the height direction of the square pipe body are subjected to fillet treatment.

6. The reagent dispensing cuvette of claim 1, wherein: the square pipe body is made of transparent high polymer materials.

7. The reagent dispensing cuvette of claim 6, wherein: the square tube body is made of polystyrene, ethylene terephthalate or methacrylate.

8. The reagent dispensing cuvette of claim 1, wherein: the square tube cap is an elastic rubber plug made of silica gel or butyl rubber.

9. The reagent dispensing cuvette of claim 1, wherein: the square tube cover comprises a cover cap (21) and a cover plug (22), and the surface of the cover plug (22) is provided with threads; after the cover plug (22) is plugged into the opening (11), the threads are tightly matched with the inner wall of the pipe body, and the cover cap (21) is exposed out of the opening.

10. The reagent dispensing cuvette of claim 1, wherein: solid powder of reaction reagents aiming at different water quality parameters is pre-filled in the tube.

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