JPS60112705A - Agent for controlling viral disease of plant - Google Patents

Abstract

PURPOSE:To provide the titled controlling agent containing skim milk and O,S- dimethyl N-acetylphosphoramidothiolate, having synergistic controlling activity, and effective to prevent the infection with virus carried by insects as well as virus infecting by direct contact. CONSTITUTION:The objective agent contains, as active components, (A) skim milk which has the effect to inhibit the direct contact infection with plant virus but is hardly expected to have the remedying effect to the contact virus and the preventive effect to the infection carrired by insects, and (B) O,S-dimethyl N-acetyl-phosphoramidothiolate of formula known as an insecticide. The agent is effective for the control of tobacco mosaic virus, potato virus Y, cucumber mosaic virus, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a plant virus disease control agent. Yo4J Rev. L < if, 4 ffi 'JJ Ha, Sum Milk (Ski+w-milk) and o,s-dimethyl N-acetylphosphoroamide thiolate (hereinafter referred to as acephate) shown by the following structural formula '7 This invention relates to a plant virus disease control agent characterized by containing a mixture of the following as an active ingredient.

0, S-dimethyl N-acetyl Acephate, which is one of the active ingredients in the present invention, is
For example, pages 61-62 and 365 of the "Pesticide Handbook 1981 Edition" (published by the Japan Plant Protection Association on July 20, 1981),
8502, 4B-34583, etc. It is an insecticide and is commonly used to control various types of stored vegetables, flower aphids, armyworms, and insects. However, the activity of acephate in controlling plant virus diseases is not known. Furthermore, there is no literature describing the use of a mixture of skim milk and 7cepheate as an agricultural chemical.

Once infected, plant disease usually causes systemic infection, causing the affected plant to wither and remain a source of infection until it is harvested or removed. I2 infection and contact infection infect other plants one after another, further expanding the damage. These plant pathogenic viruses and 1. include tobacco mosaic virus (hereinafter abbreviated as TMV), which infects plants of the Solanaceae family, and Nyagaimo virus Y (hereinafter abbreviated as PVY).
, cucumber mosaic virus (hereinafter abbreviated as CMV), which infects plants such as Solanaceae, Cucurbitaceae, and Brassicaceae.

As a means of controlling these viral diseases, insect vector-transmitted viral diseases, such as cucumber mosaic disease caused by CMV, have been conventionally used to exterminate vector insects or eliminate the same insects. t2
Attempts are being made to change the sowing time and cultivate resistant varieties in order to avoid the onset of -. However, these methods have not yet achieved sufficient control effects. In addition, efforts are being made to remove diseased plants to prevent the spread of virus diseases, but it is difficult to prevent the introduction of pathogenic insects from other fields, and it is difficult to prevent the spread of virus diseases. (I haven't done that.

On the other hand, TMV, which is transmitted through contact, is not transmitted to the inside, but it adheres to cultivation tools and soil and infects crops during cultivation management work.

To prevent this, a great deal of money and effort is spent disinfecting cultivation equipment, soil, clothing, fingers, etc. However, even with such methods, the effect of controlling viral diseases cannot be said to be sufficient.

In addition, recently attempts have been made to control tomato TMV by using the interference effect (cross protection) of closely related viruses with attenuated TMV. However, there is no guarantee that this attenuated virus is necessarily attenuated for other varieties or crops, and there is a risk that inoculated tomatoes may become a source of infection, and that the attenuated virus may not be applied to multi-type tomato seedlings. Due to problems such as the risk of contamination with highly virulent viruses during vaccination, it has not yet been widely used nationwide.

As mentioned above, the current control measures against plant virus diseases rely on physical control or cultivation control. However, its control efficacy -V is low, and
Since the damage caused by Rusu disease is ↓1: large, it is no secret that the success or failure of plant virus disease control is determining the farmers' economy.

In view of the above-mentioned viruses, the antiviral activity of a large number of natural products and synthetic compounds has been investigated for the purpose of creating plant virus disease control agents. As a result, several substances with antiviral activity were discovered. First, nucleic acid-like substances such as 2-thiouracil, 8-7 zaguanine, and 5-fluorouracil have been found as so-called growth inhibitors that suppress virus proliferation within plants. However,
Some of these substances inhibit the metabolism of nucleic acids and proteins in the host and cause phytotoxicity to plants. In addition, they are generally expensive and have low control effects in the field, so they cannot be used in fruit (trade name: Mozanone). discovered and put into practical use. However, its application is limited to tobacco TMV, and its control effect is not sufficient, and it has the disadvantage that it is not effective against aphid transmission of insect-transmissible viruses such as CMV.

On the other hand, attempts have been made to spray insecticides to control viral diseases. However, non-water-borne infectious viruses caused by aphids, such as CMV and PVY, which make up the majority of vegetable garden crops,
Because the virus can be acquired and inoculated in just a few minutes of sweat absorption, the transmission of the virus disease has already ended before the insecticidal effect appears, so we cannot expect much of a control effect. Under these circumstances, there is a strong desire for the development of new plant virus disease control agents.

Skimmed oak, which is one of the active ingredients in the present invention, can hardly be expected to have a therapeutic effect or a control effect against insect vector transmission.

The present inventors have investigated the effect of mixing with various compounds in order to compensate for the shortcomings of skim milk and create a drug that has S''S plant virus disease control effects without phytotoxicity, and has developed a novel plant We conducted extensive research to develop a plant virus disease control agent.As a result, we discovered that the above objectives could be fully met by mixing skim milk with acephate, an insecticide, and the practicality of the plant virus disease control agent of the present invention was demonstrated. I came to the conclusion that I was thinking about it.

The plant virus disease control agent of the present invention will be explained in more detail as follows. That is, the control activity of the plant virus disease control agent of the present invention is that in addition to the effect that skim milk has on infection of contact-transmitted viruses, contrary to expectations of those skilled in the art, it is effective against insect-transmitted viruses. Infection prevention I
It also has two effects. Furthermore, as is clear from the test examples below, it not only exhibits an extremely superior pesticidal effect that could not be found when each active ingredient is used alone, but also exhibits an extremely high synergistic effect of each active ingredient. This is due to its effectiveness.

Viral diseases that can be controlled by using the plant virus disease control agent of the present invention include the above-mentioned TMV, PVY, and CMV.
etc. To control viral diseases caused by these,
If it is formulated into various forms as shown below and used as an enemy to plants before the onset of viral diseases, it will have a control effect that exceeds that of conventional drugs. Compared to conventional virus disease control technology, which has no drawbacks, it reduces the work required to remove virus-infected plants during the cultivation of various crops, and also sterilizes various cultivation tools to control contact-transmitted viruses. It becomes possible to omit soil disinfection.

As described above, the plant virus disease control agent of the present invention exhibits a superior antiviral effect compared to conventional disease control agents, and is highly acceptable from the perspective of cultivation management of Ishikawa plants, as well as for one crop cultivation worker. It can be a practical pest control agent that can improve work efficiency and greatly contribute to the farm economy.

The active ingredients of the plant virus disease control agent of the present invention are mixed in a weight ratio of 1 part of skim milk to 1 part of acephene.
Although 100 parts is appropriate, the mixing ratio can be changed as appropriate depending on the conditions at the time of spraying and the situation of viral disease outbreak. In addition, other plant virus disease control agents, IP** fungicides, insecticides, herbicides, etc. can also be mixed and used.

In formulating the plant virus disease control agent of the present invention, various carriers and auxiliary agents used in the method are added to the active ingredients mixed as shown in L, and the mixture is prepared as a powder or water use agent. It can be made into a desired form such as granules.

Next, examples of formulations of the plant virus disease control agent of the present invention will be shown, but the mixing ratio of active ingredients, types and amounts of carriers and auxiliary ingredients, formulation north, etc. can be varied within a wide range. .

Note that in the examples, percentages are by weight.

''''l (Skim milk 60%, acephate 10%, sodium lauryl sulfate 1.5%, naphthalene sulfonic acid formalin condensate 2% and hydrated clay 23%
The mixture is uniformly mixed and ground to make an irrigation agent.

When using, dilute this hydrating agent 300 to 1000 times with water, and use a suitable spraying device to apply 1000 to 1000 times per 1000.
Spray at a rate of 00-3001.

---2( Skim milk 10%, acephate 2%.

0.3% of inpropyl acid phosphate, 2% of white carbon and 85.7% of clay are uniformly mixed and pulverized to form a powder.

This powder is sprayed as is at a rate of 3 to 4 kg per 10 are using a suitable spraying device.

Next, test examples will be shown regarding the effectiveness of the plant virus disease control agent of the present invention in controlling various plant virus diseases.

The present invention is not limited to the following test examples.

After sowing, the first leaves of 1411 potted cowpea seedlings (variety: Kurotane Sanshaku) were treated with a hydrating powder prepared according to Example 1 for local disease.
The lesion inhibition rate (%) was calculated using the following formula.

The test was conducted in a greenhouse in triplicate with 5 plants per section at 1 concentration.

The inoculum was tobacco (variety Sa 5) 1 month after CMV inoculation.
After grinding the mosaic leaves of M. un) with 20 times the volume of phosphate buffer, the juice obtained by filtering through gauze (equivalent to 0.8XIO-'g/m, approximately 50 localized lesions per leaf) was prepared. Using.

The results are shown in Table 1.

Table 1 Test Example 2 Cucumber mosaic virus.

), a test agent (a hydrating powder prepared according to Example 1) diluted to a predetermined concentration was sprayed on the cotyledons of a 20 m cross/bore).
After air drying, the cotyledons were inoculated with CMV in the same manner as in Test Example 1.

Fourteen days after inoculation, the number of cucumber seedlings with mosaic assembly appearing on the upper leaves was investigated, and the control value (%) was calculated according to the following formula.

The test was conducted in a greenhouse in triplicate with l@f1 and lO stocks.

The results are shown in Table 2.

Table 2, AJ! Aphids of L. 3 Shigaimovirus were fasted for about 2 hours and placed on top of a mosaic violet of a potted potato (variety: Otohei) infected with PVY, and allowed to infect by sucking for 5 minutes. Potatoes (variety Norin No. 1, No. 1,
20 days after germination), the test agent (a hydrating agent prepared according to Example 1) was sprayed on the compound leaves at 20 m/bot, and one day later, the virulent aphids were transferred to this jllg:l-.
24 It! The cells were allowed to stand for f hours and then inoculated with PVY.

Ten aphids were used for each l1l test plant.

Groups of 10 animals were kept infected and vaccinated.

In the investigation, plants showing mosaic assembly on compound leaves or streak assembly on grass leaves of inoculated potatoes (Norin No. 1) 21, 28, and 350 days after inoculation were counted as diseased plants.

This test was conducted in a greenhouse at a concentration of 20 plants.

The results are shown in Table 3.

Test 4 Tobacco mosaic virus power, potted plant 6~
Test agent (wettable powder prepared according to Example 1) diluted to various concentrations on 7-leaf stage tobacco seedlings (variety: Xanthi NC)
After air-drying, a pre-prepared TMv purified liquefied liquid (4X 10-6 g/m) was inoculated in a conventional manner.

The onset of the disease was investigated by investigating local lesions after 40 days of inoculation.
The lesion inhibition rate (%) against TMV was calculated according to the following.

The TMV solution used as the inoculum was tobacco (variety 5aIII).
ammonium sulfate salting out and ultracentrifugal force 1 no.
(It was purified and prepared for Jl use.The test was conducted in triplicate with 5 strains per 1 concentration.

The results are shown in Table 4.

Table 4 Takeshi 5 Tobacco Moikuvirus Luca tomato (variety: Toko) seeds were disinfected with a 10% aqueous solution of sodium triphosphate and sown. The selection of test tomatoes is 7.
Tomato seedling leaf juice at the ~81i stage was diagnosed with TMV antiserum, and only healthy ones were planted in a plastic greenhouse.

After tomato ^°f takes root, the height of tomato seedlings is about 50 cm.
m, the powder prepared according to Example 2 was added to 1
1 [fi] was carried out 5 times at 70 intervals at a rate of 3 kg of 0 are dust. The day after each application, a purified solution of TMV (0.4X 10-6
g/ml (see Test Example 4), the plant was contaminated, management work such as attracting buds and branches was carried out, and inoculation was artificially planned.

The hands were contaminated with TMV once for every 10 tomato plants managed.

Two weeks after the last management operation, the diseased strains were investigated, and external al;
All strains of Moi were diagnosed using TMV antiserum, and the control value (%) was calculated according to Test Example 2.

The test was conducted in triplicate using l-containing rats with 10 plants in each group.

The results are shown in Table 5.

Table 5

Claims (1)

[Claims] Skim milk and 0. A plant virus disease control agent characterized by containing S-dimethyl N-acetyl phosphoroamide thiolate as an active ingredient.