JPS6011135A - Method for extracting vital sample - Google Patents

Abstract

PURPOSE:To obtain immediately an analyzable sample with an easy operation by coating temporarily useful components of a vital sample on the surface of a solid body and eluting this coating layer with an organic solvent thereafter. CONSTITUTION:When a stopper 4 is removed and sampled blood S is dropped from a charging hole 2, surfaces of glass beads 6, 6... are coated with thin layers of useful components of the sample. In this stage, a flexible cap 5 is removed, and clean air A is blown from a discharge hole 3 to evaporate the remainder of liquid, and beads 6 are dried. The cap 5 is closed again after drying, and a certain quantity of an organic solvent O is charged from the charging hole 2, and the stopper 4 is closed, and a vessel 1 is shaked gently, and thus, object components stuck to surfaces of beads 6, 6... are eluted into the solvent. After elution, the cap 5 is removed to move the internal organic solvent to a sample cup C or the like, thus obtaining object components of a prescribed concentration.

Description

[Detailed description of the invention] B Fields of application in the technical field The present invention relates to a biological sample extraction method.

Conventional technology When extracting cholesterin or drugs from biological samples such as blood, a certain amount of the biological sample is taken into a separating funnel, an organic solvent is added and shaken vigorously, and the organic solvent is separated. An organic solvent was added, the same process was repeated several times, and the resulting organic solvent was concentrated to extract the target component.

However, since the sample is extracted with a large amount of organic solvent, it not only requires dangerous concentration work, but also requires a vibrator and liquid separation plate, making the work complicated and having to be done on the side of the analyzer. The problem was that it was not possible to do so.

C. Objective: In view of the above-mentioned problems, it is an object of the present invention to propose a novel sample extraction method that can obtain a sample that can be immediately analyzed with simple operations.

The structure of the present invention, that is, the feature of the present invention is that once the useful components of a biological sample are coated on the surface of a solid,
This coating layer is made to be eluted with an organic solvent.

E. EXAMPLES The details of the present invention will now be explained based on examples.

No. 11Δ is an external view showing an example of an extraction device used in the present invention, and No. 1 in Figure 5 is a container made of synthetic resin or glass that forms a sample extraction tube, with a sample injection port 2 at the upper end. but,
A discharge port 3 is formed at the lower end, each of which is hermetically sealed with a common stopper 4 and a flexible cap 5, and solid spherical bodies 6, 6, such as glass beads, etc. are sealed inside. . Note that the reference numeral 7 in the figure indicates a filter that prevents the spherical body 6 from flowing out.

In the device configured in this way, when the stopper 4 is removed and the collected blood S is dripped from the injection port 2 (see Figure 2), the surface of the glass beads 6. It is coated in a thin layer (Fig. 2H). At this stage, the flexible cap 5 is removed, and clean air A is blown into the discharge port 3 to disperse the excess liquid and dry it (second figure). When the drying is completed, close the cap 5 again, inject a certain amount of organic solvent O into the injection port 2, close the stopper 4, and shake the container 1 lightly. The target component attached to the solvent is eluted into the solvent (second
Figure 1 IT). After elution, by removing the cap 5 and transferring the organic solvent inside to a sample comp C or the like, the target component at a predetermined concentration can be obtained (FIG. 2 V).

In this example, glass beads are used as the solid spherical bodies, but spherical adsorbents such as silica gel may also be used.

[Example] 1: A gas chromatograph equipped with a capillary column and a packed column was prepared by using a sample obtained by mixing cholesterol and cholesteryl ester in blood obtained by the above extraction method with cholesteryl benzoate as an internal standard substance. When analyzed by
As shown in A) and (B), cholesterol (I), cholesterol bennate (II), cholesteryl CI
5: + or 2 ester (■), cholesteryl c +b:
o ester (IV), cholesteryl CIl? 1, 2, or 3 ester (V), and cholesteryl C180 ester (V7) could be detected, and it was found that the sample did not contain any impurities that would adversely affect the analysis results.

B. Effects As explained above, according to the present invention, after the useful components in the biological sample are once attached to the surface of the solid in a thin layer,
Since extraction is performed using an organic solvent, the target component can be easily extracted on the analyzer side without using a separating funnel or a shaker, allowing biological samples to be analyzed quickly and easily. be able to.

[Brief explanation of drawings]

Fig. 1 is an external view showing an example of the extraction device used in the present invention, Fig. 2 (I) to (V) are explanatory drawings showing the extraction work of the present invention, and Fig. B) is a chromatogram showing the analysis results of samples obtained according to the present invention. 1... Sample extraction tube 2... Sample injection 1" 3...
...Discharge port 4...Plug 5...Cap 6...Glass beads Applicant: Shimadzu Corporation Representative Patent attorney: Harutoshi Yotsukawa Katsuhiko Kimura Figure 7 Figure 2 m (x) (It) (y> Figure 3 (A> (B)

Claims (1)

[Claims] A step of injecting a biological sample into a container containing a large number of solid spherical bodies and coating the surface of the spherical bodies with useful components in the sample, a step of releasing excess liquid Qi, and a step of dissolving the spherical bodies in an organic solvent. A biological sample extraction method consisting of a step of immersing the sample and eluting the target component attached to the surface.