JPH0224468B2 - - Google Patents
Info
- Publication number
- JPH0224468B2 JPH0224468B2 JP57198501A JP19850182A JPH0224468B2 JP H0224468 B2 JPH0224468 B2 JP H0224468B2 JP 57198501 A JP57198501 A JP 57198501A JP 19850182 A JP19850182 A JP 19850182A JP H0224468 B2 JPH0224468 B2 JP H0224468B2
- Authority
- JP
- Japan
- Prior art keywords
- catecholamines
- alumina powder
- present
- kit
- aminomethane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000003943 catecholamines Chemical class 0.000 claims description 19
- 239000000843 powder Substances 0.000 claims description 13
- 238000001179 sorption measurement Methods 0.000 claims description 7
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
- 239000007983 Tris buffer Substances 0.000 claims description 3
- -1 ethylenediamine tetramethane Chemical compound 0.000 claims description 3
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- MFJZTKMCQFMBMA-UHFFFAOYSA-L disodium diacetate dihydrate Chemical compound [Na+].O.O.C(C)(=O)[O-].[Na+].C(C)(=O)[O-] MFJZTKMCQFMBMA-UHFFFAOYSA-L 0.000 claims 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 13
- 239000000523 sample Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000012472 biological sample Substances 0.000 description 5
- FXKZPKBFTQUJBA-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium;dihydrate Chemical compound O.O.[Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O FXKZPKBFTQUJBA-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 2
- 239000005695 Ammonium acetate Substances 0.000 description 2
- UCTWMZQNUQWSLP-UHFFFAOYSA-N adrenaline Chemical compound CNCC(O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-UHFFFAOYSA-N 0.000 description 2
- 229940043376 ammonium acetate Drugs 0.000 description 2
- 235000019257 ammonium acetate Nutrition 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- SFLSHLFXELFNJZ-QMMMGPOBSA-N (-)-norepinephrine Chemical compound NC[C@H](O)C1=CC=C(O)C(O)=C1 SFLSHLFXELFNJZ-QMMMGPOBSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- GBBUBIKYAQLESK-UHFFFAOYSA-N [3-(2-methylprop-2-enoylamino)phenyl]boronic acid Chemical compound CC(=C)C(=O)NC1=CC=CC(B(O)O)=C1 GBBUBIKYAQLESK-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000000538 analytical sample Substances 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- IZXGZAJMDLJLMF-UHFFFAOYSA-N methylaminomethanol Chemical compound CNCO IZXGZAJMDLJLMF-UHFFFAOYSA-N 0.000 description 1
- CPQCSJYYDADLCZ-UHFFFAOYSA-N n-methylhydroxylamine Chemical compound CNO CPQCSJYYDADLCZ-UHFFFAOYSA-N 0.000 description 1
- 229960002748 norepinephrine Drugs 0.000 description 1
- SFLSHLFXELFNJZ-UHFFFAOYSA-N norepinephrine Natural products NCC(O)C1=CC=C(O)C(O)=C1 SFLSHLFXELFNJZ-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000012799 strong cation exchange Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000012784 weak cation exchange Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
本発明は、生体試料からカテコールアミンを抽
出するための前処理キツトに関する。
カテコールアミンは、３・４−ジヒドロオキシ
フエニルを持つ生体アミンの総称で、これを血液
や尿等の生体試料から分析することにより疾病の
診断や治療に有用なデータを得ることができる
が、生体試料中にはごく微量しか含有されていな
いので、分析に際しては試料に含まれている夾雑
物による妨害を受け易いという問題があり、この
ため、生体試料からカテコールアミンだけを取出
すための前処理が行なわれている。この前処理
は、例えば血液に例を採ると、試料血漿を除タン
パクしたものに安定剤を添加して酵素活性を抑
え、酢酸アンモニウムで水素イオン濃度を調整し
た試料に活性化アルミナ粉末を添加し、１モルの
酢酸アンモニウム（PH9.5）を滴下してアルミナ
粉末の表面にカテコールアミンを吸着させ、遠心
脱水した後、酢酸を注入してカテコールアミンを
離脱させるようにしていた。
いうまでもなく、アルミナ粉末を活性化するに
は、酸洗いに始まつて長時間の熱処理を必要とす
る。このように、従来の前処理法は、分析試料を
得るまでに１時間半もの時間がかかるため、集団
検診等のように多数の検体を分析する場合には多
大の時間と労力を要するばかりでなく、工程が複
雑なため得られた分析試料にバラツキが生じ易い
という問題があつた。
本発明は、このような問題に鑑み、生体試料か
らカテコールアミンを簡単かつ短時間に抽出する
ことのできる前処理キツトを提供することを目的
とする。
すなわち、本発明の特徴とするところは、着脱
自在な共栓を有する筒状容器に、緩衝剤と安定剤
と活性化吸着担体を密封して保存可能とし、分析
時には栓を外して生体試料を注入するだけでカテ
コールアミンを吸着担体に捕獲した状態で得るこ
とができるようにした点にあり、以下、本発明の
詳細を図示した実施例に基づいて説明する。
第１図は、本発明の一実施例を示すキツトの外
観で、図中符号１は、遠心分離管をなすポリプロ
ピレン製筒状容器で、その上端には、ロート状の
試料注入口２が、また下端には排出口３が形成さ
れ、それぞれ共栓と可撓性のキヤツプ５により密
栓され、またその内部には活性化処理した一定粒
度のアルミナ粉末７、及び衝剤をなす0.78モルの
トリス（ヒドロキシメチル）アミノメタンと、カ
テコールアミンに対する酵素活性を抑える安定剤
をなす0.2モルのエチレンジアミン四酢酸二ナト
リウム二水塩により調整した薬剤８が封入されて
いる。なお、図中符号９は、筒状容器１内の溶液
排出時や遠心脱水時にアルミナ粉末７が流出する
のを防止するためのガラスフイルタを示してい
る。
この実施例において、予じめ作つておいたキツ
トの共栓４を外し、採取した血漿又は尿を注入口
２から滴下し、共栓４をもつて密封した後、これ
を振盪すると、トリス（ヒドロキシメチル）アミ
ノメタンは、筒状容器内をPH8.3程度に維持して、
アルミナ粉末７に選択吸着性を発揮させる。これ
によりアルミナ粉末７は、その表面にカテコール
アミンを吸着し、同時にエチレンジアミン四酢酸
二ナトリウム二水塩が酵素活性を抑える。この振
蘯を10分間ほど続けた後、筒状容器内の薬剤８を
アスピレータで排出し、下端のキヤツプ５を外し
て蒸留水を注入して、アルミナ粉末７を洗浄した
後、そのまま遠心分離器にかけて脱水する。この
ときガラスフイルタ９は、カテコールアミンを吸
着したアルミナ粉末７を捕獲して、これが流出す
るのを防止する。脱水後、再びキヤツプ５を嵌
め、注入口２から0.4規定の酢酸を注入すると、
アルミナ粉末７は吸着していたカテコールアミン
を脱離する。
〔実施例〕
緩衝液の水素イオン濃度を決定すべく、トリス
（ヒドロキシメチル）アミノメタンに濃塩酸を混
入して溶液のPHを8.2から0.1ずつ8.9まで変えたも
のを準備し、同一の血漿試料についてカテコール
アミンであるノルアドレナリンNA及びアドレナ
アリンＡの回収率の変化を液体クロマトグラフを
使用して調べたところ第２図に示したようにPH
8.2から8.9の範囲内ではほぼ一定であることがわ
かつた。また、本発明によるカテコールアミンの
回収率と従来法によるものと比較するため、0.78
モルのトリス（ヒドロキシメチル）アミノメタン
4.71gに、0.2モルのエチレン・ジアミン四酢酸二
ナトリウム２水塩2.5mlを添加し、最終量が50ml
となるように濃塩酸でPH8.3に調整したものを２
mlと、活性化アルミナ100mgを筒状容器に収容し
共栓により密封して冷暗所に保管したものに、試
料として尿を400μ注入して分析用試料を得て、
従来法と比較したところ、表１に示したように、
本発明のキツトによる前処理が非常に簡便である
にもかかわらずカテコールアミンの回収率おいて
何ら遜色のないことがわかつた。
The present invention relates to a pretreatment kit for extracting catecholamines from biological samples. Catecholamine is a general term for biogenic amines containing 3,4-dihydroxyphenyl, and by analyzing them from biological samples such as blood and urine, useful data for diagnosis and treatment of diseases can be obtained. Since catecholamines are only contained in very small amounts in samples, there is a problem that they are easily interfered with by contaminants contained in the samples during analysis.For this reason, pretreatment is performed to extract only catecholamines from biological samples. It is. For example, in the case of blood, this pretreatment involves adding a stabilizer to the deproteinized plasma sample to suppress enzyme activity, and adding activated alumina powder to the sample after adjusting the hydrogen ion concentration with ammonium acetate. , 1 mol of ammonium acetate (PH9.5) was dropped to adsorb catecholamines on the surface of the alumina powder, and after centrifugal dehydration, acetic acid was injected to remove the catecholamines. Needless to say, activating alumina powder requires pickling and then a long heat treatment. In this way, conventional pretreatment methods take up to an hour and a half to obtain an analysis sample, so when analyzing a large number of samples such as in a mass medical examination, it requires a great deal of time and effort. However, due to the complicated process, there was a problem in that the analytical samples obtained tend to vary. In view of these problems, an object of the present invention is to provide a pretreatment kit that can easily and quickly extract catecholamines from biological samples. In other words, a feature of the present invention is that the buffer, stabilizer, and activated adsorption carrier can be sealed and stored in a cylindrical container with a removable stopper, and the stopper can be removed for analysis to collect biological samples. The present invention is characterized in that catecholamines can be obtained in a state captured on an adsorption carrier simply by injection, and the details of the present invention will be explained below based on illustrated embodiments. FIG. 1 shows the external appearance of a kit showing an embodiment of the present invention. In the figure, reference numeral 1 is a polypropylene cylindrical container forming a centrifuge tube, and a funnel-shaped sample injection port 2 is provided at the upper end of the container. Further, a discharge port 3 is formed at the lower end, each of which is tightly plugged with a common stopper and a flexible cap 5, and inside the discharge port 3, activated alumina powder 7 of a constant particle size and 0.78 mol of tris as a shock agent are contained. A drug 8 prepared with (hydroxymethyl)aminomethane and 0.2 mol of ethylenediaminetetraacetic acid disodium dihydrate, which serves as a stabilizer to suppress enzyme activity against catecholamines, is encapsulated. Note that the reference numeral 9 in the figure indicates a glass filter for preventing the alumina powder 7 from flowing out during draining of the solution in the cylindrical container 1 or during centrifugal dehydration. In this example, the stopper 4 of the kit prepared in advance was removed, the collected plasma or urine was dripped from the injection port 2, the stopper 4 was sealed, and the kit was shaken. Hydroxymethyl) aminomethane is prepared by maintaining the pH inside the cylindrical container at around 8.3.
The alumina powder 7 is made to exhibit selective adsorption properties. As a result, the alumina powder 7 adsorbs catecholamines on its surface, and at the same time, ethylenediaminetetraacetic acid disodium dihydrate suppresses enzyme activity. After continuing this shaking for about 10 minutes, the drug 8 in the cylindrical container was discharged using an aspirator, the cap 5 at the bottom was removed, distilled water was poured in, the alumina powder 7 was washed, and the container was placed in a centrifuge as it was. to dehydrate. At this time, the glass filter 9 captures the alumina powder 7 adsorbing catecholamine and prevents it from flowing out. After dehydration, refit cap 5 and inject 0.4N acetic acid from inlet 2.
The alumina powder 7 desorbs adsorbed catecholamines. [Example] To determine the hydrogen ion concentration of a buffer solution, we prepared a solution in which concentrated hydrochloric acid was mixed with tris(hydroxymethyl)aminomethane and the pH of the solution was changed from 8.2 to 8.9 in 0.1 increments, and the same plasma sample was prepared. Using a liquid chromatograph, we investigated changes in the recovery rate of catecholamines noradrenaline NA and adrenaline A. As shown in Figure 2, PH
It was found that it is almost constant within the range of 8.2 to 8.9. In addition, in order to compare the catecholamine recovery rate of the present invention with that of the conventional method, 0.78
moles of tris(hydroxymethyl)aminomethane
To 4.71 g, add 2.5 ml of 0.2 mol of ethylene diaminetetraacetic acid disodium dihydrate to make a final volume of 50 ml.
2. Adjust the pH to 8.3 with concentrated hydrochloric acid so that
ml and 100 mg of activated alumina were placed in a cylindrical container, sealed with a stopper and stored in a cool, dark place, to obtain a sample for analysis by injecting 400 μ of urine as a sample.
When compared with the conventional method, as shown in Table 1,
Although the pretreatment using the kit of the present invention is very simple, it was found that the recovery rate of catecholamines was comparable.
【表】
なお、上述した実施例においては緩衝剤の濃度
を0.78モルにしているが、これに限られることは
なく、さらに吸着坦体としてアルミナ以外に硼酸
ゲル、強陽イオン又は弱陽イオン交換樹脂を使用
することができる。
以上、説明したように本発明において、着脱自
在な共栓、及びキヤツプにより注入口、及び排出
口が密封可能で、かつ前記排出口側に粉体濾過用
のフイルタ材を収容した容器に、トリス（ヒドロ
キシル）アミノメタンと、エチレンジアミン四酢
酸二ナトリウム二水塩と、カテコールアミン吸着
担体とを封入したので、不使用状態にあつては安
定化剤により吸着担体の活性状態を長期間維持す
ることができるばかりでなく、容器に予め収容さ
れている規定の薬剤量で、かつ同一の容器内で複
数の処理を済ませることができるため、前処理条
件が安定して分析精度と信頼性の向上を図ること
ができ、さらにはキツトを用意するだけで多数の
サンプルを同時に前処理することができる。[Table] In the above-mentioned example, the concentration of the buffer is 0.78 mol, but the concentration is not limited to this. In addition to alumina, boric acid gel, strong cation or weak cation exchange can be used as the adsorption carrier. Resin can be used. As explained above, in the present invention, the inlet and the outlet can be sealed with a removable common stopper and a cap, and the container containing the filter material for powder filtration on the side of the outlet is provided with Tris. Since (hydroxyl)aminomethane, disodium ethylenediaminetetraacetic acid dihydrate, and catecholamine adsorption carrier are encapsulated, the active state of the adsorption carrier can be maintained for a long period of time with a stabilizer when not in use. Not only that, but multiple treatments can be completed in the same container with a specified amount of drug stored in the container in advance, which stabilizes pre-processing conditions and improves analytical accuracy and reliability. Furthermore, it is possible to preprocess many samples at the same time just by preparing a kit.
第１図は、本発明の一実施例を示す前処理キツ
トの外観図、第２図は、本発明によるカテコール
アミンの回収率を示す説明図である。
１……筒状容器、７……活性化アルミナ粉末、
９……ガラスフイルタ。
FIG. 1 is an external view of a pretreatment kit showing one embodiment of the present invention, and FIG. 2 is an explanatory diagram showing the recovery rate of catecholamine according to the present invention. 1... Cylindrical container, 7... Activated alumina powder,
9...Glass filter.
Claims (1)
び排出口が密封可能で、かつ前記排出口側に粉体
濾過用のフイルタ材を収容した容器に、トリス
（ヒドロキシルメチール）アミノメタンと、エチ
レンジアミン四酢酸二ナトリウム二水塩と、カテ
コールアミン吸着担体とを封入してなるカテコー
ルアミンの分析用前処理キツト。1. Tris(hydroxylmethyl)aminomethane and ethylenediamine tetramethane are placed in a container whose inlet and outlet can be sealed with a removable stopper and cap, and which contains a filter material for powder filtration on the outlet side. A pretreatment kit for analysis of catecholamines, which includes disodium acetate dihydrate and a catecholamine adsorption carrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19850182A JPS5987366A (en) | 1982-11-11 | 1982-11-11 | Pretreatment kit for analyzing catecholamine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19850182A JPS5987366A (en) | 1982-11-11 | 1982-11-11 | Pretreatment kit for analyzing catecholamine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5987366A JPS5987366A (en) | 1984-05-19 |
| JPH0224468B2 true JPH0224468B2 (en) | 1990-05-29 |
Family
ID=16392177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19850182A Granted JPS5987366A (en) | 1982-11-11 | 1982-11-11 | Pretreatment kit for analyzing catecholamine |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5987366A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06302352A (en) * | 1993-04-19 | 1994-10-28 | Yazaki Corp | Waterproof connector |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100772970B1 (en) | 2006-06-30 | 2007-11-02 | 메디칸(주) | Centrifuge and centrifuging method |
| JP6236874B2 (en) * | 2013-05-24 | 2017-11-29 | 栗田工業株式会社 | Anionic polymer concentration measuring method and apparatus |
| WO2016098757A1 (en) * | 2014-12-15 | 2016-06-23 | 積水メディカル株式会社 | Method for detecting amino acids or acylcarnitine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56133649A (en) * | 1980-03-24 | 1981-10-19 | Japan Spectroscopic Co | Method and device for measurig catechol amines using natural fluorescent method |
| JPS57191548A (en) * | 1981-05-20 | 1982-11-25 | Yanagimoto Seisakusho:Kk | Analysing apparatus of catechol amine |
-
1982
- 1982-11-11 JP JP19850182A patent/JPS5987366A/en active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56133649A (en) * | 1980-03-24 | 1981-10-19 | Japan Spectroscopic Co | Method and device for measurig catechol amines using natural fluorescent method |
| JPS57191548A (en) * | 1981-05-20 | 1982-11-25 | Yanagimoto Seisakusho:Kk | Analysing apparatus of catechol amine |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06302352A (en) * | 1993-04-19 | 1994-10-28 | Yazaki Corp | Waterproof connector |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5987366A (en) | 1984-05-19 |
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