JPS6010711B2 - Ultrasonic cell crushing device - Google Patents

Abstract

PURPOSE:To provide the titled apparatus capable of simultaneously disintegrating a plurality of specimens to be disintegrated, e.g. erythrocyte, etc. using a single apparatus, by mounting a secondary vibrator having a specific shape detachably to the top of each specimen container. CONSTITUTION:The excitation coil 3 is excited by the oscillator 1 to drive the vibrator 2 and generate ultrasonic radiation. The ultrasonic vibration is transmitted through the liquid in the tank 4, and transferred through the walls of the containers 8a, 8b, 8c,... to the liquid S containing of erythrocytes, etc. to be disintegrated and to the secondary vibrator 9. Consequently, the erythrocytes, etc. are disintegrated by the cavitation produced in the liquid S. Since a plurality of the containers 8a, 8b, 8c... can be held vertically in the tank 4, a plurality of specimens can be disintegrated using a single apparatus. As the large-diameter part 10 of the secondary vibrator 9 is placed on the top rim 13 of the container, and the small-diameter part 11 is brought into close contact with the inner surface 14 of the container, the escape of the mist from the container can be perfectly prevented, and the operation can be carried out under the bacteriologically clean conditions.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an apparatus for disrupting cells, etc. using ultrasonic waves, and more particularly to an apparatus for disrupting red blood cells, cells, viruses, tissues, etc. using ultrasonic waves.

Conventionally, the 6th method was used to crush red blood cells etc. using ultrasonic waves.
Devices such as those illustrated in the figure are often used.

That is, a high frequency oscillator 1', a vibrator 2', and a horn 3'
For example, the horn 3' is immersed in a beaker 4' containing a liquid containing red blood cells obtained by centrifugation from blood, and in that state, the vibrator 2' is excited by the output of the high-frequency oscillator 1'. Ultrasonic vibrations are applied to the red blood cells in the beaker 4' via the horn 3' to cause cavitation and the like to crush the red blood cells. Although this ultrasonic disintegration device has achieved considerable effects in various fields such as on-site medical care and pathological research, it still has the following points to be improved.

Namely, one horn 3' is associated with one beaker 4', etc. containing a liquid containing objects to be crushed such as red blood cells, so only one object can be crushed by one crushing device. Yes, multiple pieces cannot be crushed at the same time. This causes inconvenience in on-site medical care. In addition, in the process of crushing objects such as red blood cells by ultrasonic action, the objects to be crushed become atomized, but in the conventional case, a horn 3' is placed in a beaker 4'.
Since the beaker 4' is simply immersed and the beaker 4' is opened as is, there is a risk that the atomized particles may be scattered from the beaker 4'.
It is dangerous for testers and researchers. Furthermore, in order to apply effective ultrasonic action, it is necessary to position the horn 3' at the center of the object to be crushed, such as red blood cells, in the beaker 4'.
In particular, since there is no means for positioning the pupil, it may not always be possible to position the pupil in the center, and positioning may be surprisingly time-consuming. There are several points that should be improved.

The present invention has been made in view of the above points, and includes an ultrasonic generating device comprising an oscillator for driving a vibrator, a vibrator for generating ultrasonic waves, and a liquid-filled liquid. , a plurality of containers positioned in a vertical manner in a liquid within the tank;
The top of each container consists of a large-diameter portion that sits on the edge of the top entrance of each container, and a 4-diameter portion that is inserted closely into the inner peripheral surface of the top of the container, and a metal rod is suspended from the center. This is an ultrasonic cell disruption device that is equipped with a secondary transducer that can be inserted and removed, and its purpose is to:
At the same time, it is possible to crush a plurality of objects to be crushed, such as red blood cells.

In addition, by preventing the atomized particles generated during the crushing process from scattering to the outside, and by ensuring that the metal rod that functions as a secondary vibrator is positioned at the center of the container. The object is to be able to effectively apply ultrasonic vibration to the object to be crushed at any time. Next, an embodiment of the present invention for achieving the above object will be described in detail with reference to the accompanying drawings.

1 is a high frequency oscillator, 2 is a vibrator such as a ferrite magnetostrictive vibrator around which an excitation coil 3 driven by the high frequency oscillator 2 is wound, and 4 is a pot filled with liquid W. This is a well-known device that generates ultrasonic waves.

The present invention is characterized in the following points.

That is, a plurality of through holes 6a, 6b...
... A lid plate 7 on which 6f is formed is placed on top of the tank 4. And the above-mentioned through holes 6a, 6b...
. . . Each of the 6f has a container 8 made of glass or plastic, such as a long and narrow container called a spitz.
Insert a, 8b, 8c....... into the rack,
Each of the containers 8a, 8b, 8c......
immersed in the liquid W in a vertical manner. Each container 8a, 8
b, 8c........., the green thick part of the top opening is the through hole 6a, 6b...... of the diamond plate 7, respectively.
...It is held in a hanging manner by engaging with 6f. And each container 8a, 8b, 8c......
The following secondary vibrators are removably inserted into each of them.

That is, as illustrated in FIG.
0 and a small part 11 that extends below the large diameter part 10, and a metal rod 12 hanging vertically at the center of the large part 10 and the small diameter part 11, and the whole is made of metal such as stainless steel. When this secondary vibrator 9 is inserted into a container with the metal thread 12 facing downward, the large cross section 10 is seated on the top entrance rim 13 of the container 8a, and the small diameter section 11 is Each diameter is determined so that the metal rod 12 is inserted almost closely to the inner circumferential surface 14 at the top of the container 8a, and when the metal rod 12 is inserted to the end, the tip 15 of the metal rod 12 is attached to the container 8a. The length of the metal twill 12 is determined so that it does not come into contact with the bottom 16 of 8a. The secondary oscillator 9 is caused to resonate by the longitudinal vibration generated by the oscillator 2 functioning as a primary oscillator, and the length, thickness, and overall size of the secondary oscillator 9 are etc. are determined to have natural frequencies that satisfy the above-mentioned better resonance conditions.

The shape of the tip 15 of the metal bar 12 constituting the secondary vibrator 9 can be, for example, straight as shown in the third figure; in,
Tapered tip 1 that gradually becomes thinner as shown in Figure 4
7, or fine grooves 18 may be carved on the circumferential surface of the tapered tip as shown in the fifth figure. Based on the above configuration, the operation of crushing objects to be crushed, such as red blood cells, will now be explained.

First, a liquid S containing red blood cells and the like to be crushed is placed in each of the containers 8a, 8b, 8c, . . . .

Next, the metal rod 12 is inserted into the container, and the secondary vibrator 9 is inserted so that the large diameter portion 10 is on the top opening edge 13. In the inserted state, the tip 15 of the metal rod 12 is immersed in the liquid S of the object to be crushed. Then, the containers 8a, 8b, 8c into which the secondary vibrator 9 is inserted...
・through holes 6a, 6 of the lid 7 mounted on the rice cake 4, respectively.
Insert into b, 6c...... Hanging containers 8a, 8b, 8c when arriving at Tachibana...
...... is immersed in the liquid W in tank 4. FIG. 1 shows this state. In this state, the oscillator 1 is driven to vibrate the vibrator 3 through excitation of the vibrating coil 3, thereby generating ultrasonic waves. The generated ultrasonic vibrations propagate through the liquid W in the container 4, and each container 8a. 8b, 8c・
It is transmitted to the liquid to be disrupted S containing red blood cells and the like in each container and the secondary vibrator 9 through the walls of the container. As a result, cavitation occurs in the liquid S containing objects to be crushed such as red blood cells, and the cavitation causes the red blood cells to be crushed. However, what is more characteristic is that the length, thickness, overall shape, etc. of the secondary vibrator 9 are determined so that it has a natural frequency that resonates with the frequency of the vibrator 2. , the secondary vibrator 9 is sharply synchronized with the vibration of the vibrator 3, and ultrasonic vibrations are transmitted from the tip of the metal rod 12 to the liquid S of the object to be crushed, thereby further promoting the occurrence of cavitation and transmitting ultrasonic waves. The pressure generation caused by the vibration is further promoted, and objects to be crushed such as red blood cells are crushed more quickly and effectively. Now, the features of the present invention in such a crushing process are as follows.

One accommodates the object S to be crushed, such as red blood cells, and a secondary vibrator 9.
Containers 8a, 8b, 8c with inserted...
Since a plurality of objects S can be held in the cage 4 in a vertical manner, a plurality of objects S to be crushed can be simultaneously crushed with one device.

Therefore, it is a great contribution to medical care. The other is that when the object S to be crushed such as red blood cells is accommodated and then the secondary vibrator 9 is inserted, the large and light part 10 of the secondary vibrator 9 sits on the top closing edge 13, and especially the small diameter part 11 Since it is in close contact with the inner circumferential surface 14, the misty female position is in contact with the container 8a, 8b or 8c.
...There is no risk of it scattering to the outside. Therefore, work can be carried out in bacteriologically clean conditions.

Yet another feature is that when the secondary vibrator 9 is inserted, since the metal rod 12 is vertically placed in the center, it is necessarily located in the center of the container. Therefore, energy by ultrasonic vibration can be applied to the object S to be crushed more effectively and without waste. As detailed above, the present invention provides an apparatus for generating ultrasonic waves comprising an oscillator 1 for driving a vibrator 2, a vibrator 2 for generating ultrasonic waves, and a liquid-filled chamber 4. A plurality of containers 8a, 8b, 8c vertically positioned in the liquid W in the tank 4. Each of the containers 8a, 8b, 8c.・…．・・・． ．．・・・・・・
It consists of a large-diameter portion 10 that sits on the top rim 13 of the container, and a large-diameter portion 11 that supports the inner peripheral surface 14 of the top of the container, and has a metal rod 12 in the center. Since it is equipped with a flexible secondary vibrator 9, it is possible to simultaneously crush multiple objects such as red blood cells and cells with a single device, which contributes to the rationalization of work in the medical field. It's large. In addition, it is bacteriologically clean because it prevents the scattering of twilled particles. Furthermore, the secondary vibrator can always be positioned at the center of the container, and the energy of ultrasonic vibration can be evenly and effectively transmitted to the object to be crushed, allowing for better crushing. It offers various practical advantages.

[Brief explanation of the drawing]

The accompanying drawings show embodiments of the present invention, with FIG. 1 being a longitudinal cross-sectional view of the entire device, FIG. 2 being a plan view of the device with the container removed, and FIG. 3 showing an example of the container and the secondary vibrator. Longitudinal side view, 4th
The figure is a side view of another example of a secondary vibrator, Figure 5 is a side view of another example of a secondary vibrator, and Figure 6 is a diagram of a conventional example. Vibrator, 3 is excitation coil, 4
5 is a case, 6a to 6f' are through holes, 7 is a cover plate,
8a to 8c are containers, 9 is a secondary oscillator, 10 is a large take part, 1
1 is a small light part, 12 is a metal rod, 13 is a top edge, 14 is an inner peripheral surface, 15 and 17 are tips, 16 is a container bottom, 18 is a groove,
W indicates the liquid in the chamber, and S indicates the object to be disrupted, such as a liquid containing red blood cells. Dream' figure 2 figure 3 kata 4 figure hair 5

Claims (1)

[Scope of Claims] 1. In an apparatus for generating ultrasonic waves consisting of an oscillator 1 for driving a vibrator 2, a vibrator 2 for generating ultrasonic waves, and a tank 4 filled with liquid, the tank 4 is a plurality of containers 8a, 8b, 8c, . . . positioned in a vertical manner in the liquid W therein; a large diameter portion 10 sitting on the top opening edge 13 of each of the containers 8a, 8b, 8c, . It consists of a small-diameter portion 11 that is in close contact with the inner circumferential surface 14 of the top of the container, and a metal rod 12 is suspended from the center, and is equipped with a secondary vibrator 9 that can be freely inserted and removed from the top of the container. Crushing equipment. 2 In the invention described in claim 1, the metal rod 1
An apparatus for disrupting cells, etc. using ultrasonic waves, characterized in that the tip 15 of the tip 2 is straight. 3 In the invention described in claim 1, the metal rod 1
A device for disrupting cells, etc. using ultrasonic waves, characterized in that the tip of the tip 2 is a tapered tip 17 that becomes gradually thinner as it goes forward. 4 In the invention described in claim 3, the metal rod 1
An apparatus for disrupting cells, etc. using ultrasonic waves, characterized in that fine grooves 18 are formed on the outer peripheral surface of the tapered tip of No. 2.