JPS60105489A - Production of medium for lactobacillus starter - Google Patents

Abstract

PURPOSE:To produce a medium for a lactobacillus starter having phage resistance and the proliferating effect to the lactobacillus, by removing a part of calcium from the milk components, and adding the sodium or potassium salt of a specific organic acid to the components. CONSTITUTION:Defatted milk or a solution of defatted milk powder is boiled in the presence of an orthophosphate, and centrifuged to remove >=20%, preferably >=40%, especially about 50% of the calcium content in the milk components. The obtained supernatant liquid is added with the sodium or potassium salt of an organic acid selected from succinic acid, malic acid, tartaric acid, and acetic acid, and sterilized by heating. The sterilized product is stored in the form of liquid or in powdery form after concentration and spray drying.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a cultivation method for lactic acid bacteria starter.

More specifically, the present invention has phage resistance, and
The present invention relates to a method for producing a lactic acid bacteria starter medium that has the effect of proliferating lactic acid bacteria.

Generally, when producing fermented dairy products using lactic acid bacteria such as cheese, yogurt, and lactic acid bacteria drinks, sterilized skim milk is inoculated with lactic acid bacteria suitable for various uses, and the resulting lactic acid bacteria culture is used as a lactic acid starter (hereinafter referred to as A widely used method is to add it to each main ingredient as a starter (simply referred to as a starter) and carry out the main fermentation.

In addition, sterilized skim milk (
(yeast extract is added as needed) has been widely used, but when sterilized skim milk is used as a starter medium, lactic acid bacteria are susceptible to growth and damage from contaminating phages, and once contaminated with phages. The number of lactic acid bacteria decreases dramatically, making it impossible to use it as a starter.

The present inventors conducted research on a starter medium that can increase the number of lactic acid bacteria and the vitality of the bacteria at the same time as providing phage resistance.As a result, the present inventors found that they were able to provide phage resistance by removing part of the calcium in milk components. It was discovered that the number of lactic acid bacteria and the vitality of the bacteria could be increased by adding sodium or potassium salts of succinic acid, malic acid, tartaric acid, and acetic acid.

The present invention involves adding orthophosphate to skim milk or skim milk powder solution, boiling it, centrifuging it to remove part of the calcium in the milk components, and then adding cono-phosphoric acid, malic acid, tartaric acid,
This is a method for producing a culture medium for lactic acid bacteria starter, which is characterized by adding one or more types of sodium salt or potassium salt of an organic acid consisting of acetic acid and sterilizing the medium, or by spray drying after concentration.

In the present invention, firstly, in order to make the starter medium phage resistant, the calcium content in the medium must be at least 20%.
% is removed.

FIG. 1 is a diagram showing the relationship between the amount of phosphoric acid (expressed as P) added to skim milk 1002 and the calcium residual rate in the supernatant. To measure the calcium residual rate in Figure 1, dilute 100v of skimmed milk with disodium phosphate-hydrogen (Omg ~ 150cm as P) in water.
This is done by adding Ct to a pH of about 68, heating at 100°C for 30 minutes, centrifuging, and measuring the amount of calcium in the supernatant.

As is clear from FIG. 1, calcium in skim milk is removed in 70 minutes by Or) IJ salt.

In the present invention, 20% or more of calcium is removed from skim milk or skim milk powder solution by utilizing the decalcification effect of orthophosphate.

If 20% or more, preferably 40% or more, and more preferably about 50% of calcium is removed from skim milk or skim milk powder solution, phage proliferation can be suppressed.

In the present invention, in order to partially remove calcium, a salt of phosphoric acid is added to skim milk or a solution of powdered skim milk and boiled, and calcium forms a salt with phosphoric acid and precipitates. Orthophosphate is phosphoric acid-hydrogen disu)
Preferably, a solution of IJum dissolved in water and brought to a PT-T of about 68 with hydrochloric acid is used. The amount of orthophosphate to be added is determined by converting the amount of phosphorus according to the desired amount of calcium removal from the residual ratio of calcium shown in FIG.

The salts of IJ acid (calcium and ol) are separated by centrifugation (3
) to obtain a supernatant.

In the present invention, 20-70% of the calcium in the milk component is removed and phage resistance is imparted, so it can be used as a starter medium as it is, but in order to increase the growth effect of lactic acid bacteria, the supernatant Succinic acid in the liquid,
One or more sodium salts or potassium salts of organic acids consisting of malic acid, tartaric acid, and acetic acid are 0.05 to 0.
．． 5M1, preferably about 0.1M.

The addition of sodium or potassium salts of these organic acids
It shows the effect of increasing the buffering power of the medium and increasing the growth rate and activity of lactic acid bacteria.

After adding the sodium salt or potassium salt of an organic acid to the supernatant, heat sterilize it at 121°C for about 15 minutes and store it as a liquid, or concentrate and spray dry it and store it in a powdered emulsion. I can do it.

The liquid or milk powder obtained here is excellent as a starter culture medium. This starter medium is
It has phage resistance and has the effect of increasing the growth rate and activity of lactic acid (4) bacteria.

Next, test examples and examples of the present invention will be shown.

Test example 1゜Skim milk powder 10. Dissolve Of in 90°C of water, add 10mA of orthophosphate solution (75μ as P) whose pH was adjusted to 6.8 with phosphoric acid-disodium hydrogen solution and dilute hydrochloric acid, heat at 100°C for 60 minutes, and centrifuge. After separation, skim milk from which about half of the calcium has been removed is obtained. Heat this to 121℃15
Streptococcus e lactis and Streptococcus cremoris were inoculated separately into a solution prepared by heat sterilization for several minutes, and the insect acidity was examined by phage contamination.
In this medium, calcium was removed, so phages could not proliferate, and the acidity of lactic acid bacteria did not decrease at all, whereas in the skim milk medium that was not subjected to this treatment, the lytic action of phages reduced the acidity of lactic acid bacteria. .

Test Example 2: 100 f of skim milk was mixed with 1 ov of orthophosphate solution (PH of 6.8 using disodium phosphate-hydrogen solution and dilute hydrochloric acid).
7511v) was added, heated at 100°C for 30 minutes, and centrifuged to remove about half of the calcium. Sodium salts of malic acid, succinic acid, lactic acid, citric acid, tartaric acid, and acetic acid were added to the skim milk to a concentration of 0.1M. Streptococcus lactis and Streptococcus cremoris were inoculated separately, and cultured at 25° C. for 24 hours. The pH of the medium, the number of bacteria, and the insect acid activity were measured.

In addition, insect acid activity refers to culture solution 2- taken and sterilized skim milk 1-
00fnt and cultured at 60°C for 6 hours.
It is expressed as (lactic acid%).

In addition, as controls, a Ca-removed medium but no added organic acid and a skim milk medium were used.

The results are shown in Table 1 below.

Furthermore, succinic acid, malic acid, tartaric acid, and acetic acid (IJ) salts were added to regular skim milk at a concentration of 0.1 M, and the insectic acid activity when incubated at 30°C for 6 hours is shown in Table 2. It will be done.

Table 1: Growth rate and activity of lactic acid bacteria in calcium-removed skim milk containing various organic acid salts (7) Table 2: Activity of lactic acid bacteria in skim milk containing various organic acid salts (8) As is clear from Table 1, the addition of succinic acid and malic acid is effective in maintaining high bacterial counts and activity after 24 hours at 25°C, followed by tartaric acid and acetic acid, and lactic acid is the most effective. There was almost no effect, and citric acid was observed to have an inhibitory effect.

On the other hand, as is clear from Table 2, when calcium in skim milk was not removed, no inhibitory effect on lactic acid bacteria was observed even when citrate was added.

When calcium is removed from skim milk in this way,
The addition of succinate, malate, tartrate, and acetate was effective in increasing the activity of lactic acid bacteria.

Example 1 Skim milk powder 1011 was dissolved in 900 kg of warm water, and disodium phosphate-hydrogen crystals (N a 2 HP O
4゜12H,O) To 8.67, add water to 40, and to 9, add diluted hydrochloric acid (t'pH 68), and heat at 100℃ for 30
Heat for a minute.

After cooling, centrifuge to remove about half of the calcium in the milk, plus 100% yeast extract? Add 9 to the sodium succinate crystals 27, mix and dissolve, and heat sterilize at 121° C. for 15 minutes to obtain a liquid medium for lactic acid bacteria starter. This sterilized liquid medium is Str, Iactis%Str.

cremoris, Lac, )Lelveticu
When used as a culture medium for lactic acid bacteria starters such as S., Leuconostoccitrovorum, etc., the number of bacteria,
It had high activity and high phage resistance, making it an excellent medium for lactic acid bacteria starters.

Example 2 Disodium phosphate-hydrogen crystals (N
Add and dissolve a2HPO4-12H20)8, and adjust the pH to 6.7 by adding dilute hydrochloric acid. 40 at 100'C
After heating for 50 minutes, centrifuge to remove about half of the calcium. Add yeast extract I OOf, D, T, IJ to this.
Add 17.8 kg of sodium malate crystals and mix and dissolve. After heat sterilizing at 75°C for 10 minutes, the mixture is concentrated and spray-dried to obtain milk powder. This powdered milk is made into a 10% solution, and 121
After sterilizing at ℃ for 15 minutes, 8tr, thermophilus, 8tr,
acidopk+lus.

Str, Iactis, Str, cremor
When used as a starter medium for lactic acid bacteria such as is, the bacterial count and activity were both high, making it an excellent starter medium.

Example 3 Add 9 kg of warm water to 100~100~ of skim milk powder, dissolve it, and add phosphoric acid-hydrogen dichloromethane crystals (Na
2) Dissolve 11η4・12H20)6# in 30 kg of water, add a solution adjusted to pH 6.8 with dilute hydrochloric acid, and add 10
Heat at 0°C for 1 hour. After cooling, it is centrifuged to remove about 40% of the calcium in milk.

To this, add 8 Icg of DL-sodium malate crystals, 13 kg of sodium succinate crystals, and further add 5 kg of yeast extract.
Add 10 kg of 0f1 purified lactose, mix and dissolve, and heat to 75″C.
After continuous sterilization for 15 seconds, it is concentrated and spray-dried to form milk powder. This powdered milk was made into a 11% solution and 121'c
When used as a starter medium for various lactic acid bacteria after sterilization for 15 minutes, the number of bacteria and activity were both high, and the phage resistance was also high, making it an extremely excellent medium for lactic acid bacteria starters.

[Brief explanation of drawings]

FIG. 1 is a diagram showing the relationship between the amount of disodium phosphate (indicated as P) added to skim milk 1002 and the calcium residual rate in the supernatant. Figure 1 (Part) Amount of phosphorus added to 100g of skim milk

Claims (1)

[Claims] After adding IJ salt to skim milk or skim milk powder solution and boiling it, centrifugation is performed to remove part of the calcium in the milk components, and then organic acid consisting of succinic acid, malic acid, tartaric acid, and acetic acid is added. A method for producing a culture medium for lactic acid bacteria starter, which comprises adding one or more sodium salts or potassium salts and sterilizing the same, or concentrating and then spray drying.