JPS596952A - Rotary seal structure for flowing sample into rotary container and discharging the same therefrom - Google Patents

Abstract

PURPOSE:To prevent the rising in the temp. of blood components, by a method wherein a slide surface interposed between a stationary part and a rotary container is formed into an anti-wear structure holding airtightness during sliding and a passage for centrifugally separated blood components is provided at a position avoiding a slide part. CONSTITUTION:In a centrifugal separator container, an anti-wear seal plate 7 is interposed between a stationary part having an inlet 2 and an outlet 3 for a sample (e.g., blood) from the outside and a rotary container 4 to be slid therewith. Blood introduced from the inlet 2 is freely fallen to a sump part 11 and enters the container 4 through an opening part 5 by centrifugal force to be subjected to centrifugal separation. As the result, a separated layer is formed successively in the order of erythrocyte, leukocyte and serum from the outer peripheral surface. Corresponding to the amount of blood entering from the inlet 2, serum is at first discharged from the outlet 3 through an opening part 6, vertical grooves 13, 14 and a ring groove 12. This passage is provided at a position avoiding the vicinity where friction heat is generated by sliding. By this mechanism, the rising in the temp. of blood components is prevented.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a structure of a rotary seal part in a centrifugal separator for separating and extracting blood components so that the temperature of the blood components to be taken out does not increase.

1 and 2 show examples of prior art. Take blood as an example of a sample to be inflowed and outflowed. The rotary seal structure of the continuous centrifugal separator shown in Figure 2 consists of a stationary member 1 having a blood inlet 2 and an outlet 6, and a sol plate 7 made of wear-resistant material fixed to a rotating container 4 (a seal in Figure 2). The cylinder) and the cylinder 8 slide in close contact with each other to maintain airtightness. Figure 1 shows an example in which the glue 11 is provided at the center of rotation. In this example, the outflow port 6 is located at a position 1c off the center, and a ring-shaped groove 9 is provided on the side of a stationary member (or a rotating member) to communicate with the opening B. The contact pressure between the stationary member 1 and the sliding seal plate 7 is (applied by the spring 10.
Blood V entering from 2. Reservoir 1 provided at the bottom of the center of rotation.
After falling freely into the rotating container 4, it is blown away by the centrifugal force through the opening 5VC and guided into the rotating container 4. Next, the blood is separated in the order of red blood cells, white blood cells, and plasma from the outer circumferential side, and the separation layer swells, and as the amount of blood entering the population increases, the separation boundary layer moves to the center of rotation, and finally the opening 9. 6, passes through the sliding seal plate 7 and passes through the ring groove 9 of the stationary part to the -C outlet 6.
More plasma comes out. Since the proportion occupied by white blood cells is small, an easy way to extract white blood cells with high accuracy is to reduce the movement speed of the white blood cell layer to make detection easier. Everything other than the white blood cell layer may be returned to the donor, so if the blood is passed through the heated sliding part at an extremely low speed, the blood (other than white blood cells), which is at a high temperature compared to body temperature, will be returned.
Harm is expected. Furthermore, even if the white blood cell layer is collected for use by a patient without being returned to the human body, care must be taken not to expose it to higher temperatures than necessary.

SUMMARY OF THE INVENTION An object of the present invention is to eliminate the above-mentioned drawbacks of the prior art and to take measures to prevent temperature rise even when a continuously centrifuged product, such as a blood component, is taken out at a slow rate.

The purpose of the present invention is to improve the rotary seal structure of a continuous centrifugal separator, which has passages that communicate with each other while maintaining airtightness between a stationary part and a rotating container. In blood component separation where temperature rise must be kept as low as possible, it is necessary to prevent temperature rise even when the sample is taken out at a low speed that allows accurate collection of the area near the separation boundary layer. For this purpose, the sliding surface interposed between the stationary part and the rotating container has a wear-resistant structure with the main purpose of maintaining airtightness during sliding, and the blood passage is directly connected to the sliding part that becomes the heating element. The structure is such that it is installed in a position that avoids

The stationary member 1 has an inlet 2 and an outlet 6, and a rotating container 4
The sliding seal plate 7 made of N wear and tear resistant material is

It is fixed with pin 15. Stationary member 1 and sliding seal plate 7
slides on surface 8 under suitable contact pressure.

Also, an O-ring 16 is connected between the stationary member 1 and the Nand 18 screwed into the rotating container 4 on one side of the ring-shaped sliding seal plate 17.
It performs υ good sealing and contact pressure addition action. At this time, the ring-shaped sliding seal plate 17 rotates together with the rotating container 4 due to the friction of the O-ring 16, and the stationary member 1
A sealed sliding operation is performed.

In this way, the blood that entered from the inflow 1''2 flows into the reservoir 111/il.
: It falls freely, reaches the opening 5 due to centrifugal force, enters the rotary evaporator, and undergoes centrifugal separation. As a result, separated layers are formed in the order of red blood cells, white blood, and plasma from the outer circumferential side. Depending on the amount of blood entering from the inlet 2, plasma first flows through the opening 6 and into the vertical groove 16.
14, and exits through the ring groove 12 to the outflow n3. This passage is provided at a position close to where frictional heat due to sliding is generated.

In the case of leukocyte collection, since leukocytes make up a small proportion of whole blood, it is difficult to detect the boundary between white blood cells and red blood cells if the extraction rate is too fast, and it is also difficult to detect the boundary between white blood cells and red blood cells. be. 4'+V If you want to take out white blood cells as often as possible, an effective strategy is to take them out as slowly as possible to make it easier to detect the boundary layer. However, if the outflow speed is made extremely slow, the conventional structure in which the outflow port passes through the sliding seal 4Ji7 has the disadvantage that the temperature of the outflowing blood components tends to rise. In the present invention, since the sliding portion 8'Jt is avoided as the outflow path, the temperature of the blood components does not rise.

In the case of continuous blood component collection, it is best to extract the desired white blood cell component from the blood sample taken from a healthy person using a knife, and then return the remaining plasma and red blood cells to the same temperature as body temperature using internal VCY water. it is conceivable that. The collected white blood cell components are injected into the patient, and those with a high number of granulocytes are said to be more effective.

) It is said that the survival time of granulocytes is extremely short, so there is no way to avoid exposing them to temperatures higher than body temperature.

[Brief explanation of drawings]

FIGS. 1 and 2 are longitudinal sectional views showing conventional seal structures, respectively. FIG. 6 is a longitudinal cross-sectional view showing an embodiment of the present invention, and FIG. 4 is a cross-sectional view taken along the line A--A in FIGS. 1 is silence + f1 member, 2 is inflow 11, 3 is outflow [-J,
4 is a rotating container, 5 is an opening, 7 is a sliding seal plate, 8 is a sliding part, 9 is a ring groove, 10 &;t! In, 11 is the reservoir part, 12 is the ring groove, 13 & ↓ table load, 1
4 is a vertical groove, 15 is a bottle, 16 is an 0 link, 17 is a ring-shaped sliding seal (da, 11j is a nut. Name of patent applicant Hitachi Koki Co., Ltd. Fig. 1 □ Fig. 2 Fig. 3 1 year old 4 figures

Claims (1)

[Claims] In a centrifugal separation container, a wear-resistant seal plate is interposed and slid between a stationary part having an inlet and an outlet for an external sample and a rotating container to create a sample flow path that communicates between the stationary part and the rotating part while maintaining airtightness. A rotary seal structure characterized in that the wear-resistant seal plate is formed with one communication port at a position other than the sliding surface of the wear-resistant seal plate.