JPS5952977B2 - Production method of turbidity standard solution for nephelometric determination - Google Patents
Production method of turbidity standard solution for nephelometric determinationInfo
- Publication number
- JPS5952977B2 JPS5952977B2 JP9167179A JP9167179A JPS5952977B2 JP S5952977 B2 JPS5952977 B2 JP S5952977B2 JP 9167179 A JP9167179 A JP 9167179A JP 9167179 A JP9167179 A JP 9167179A JP S5952977 B2 JPS5952977 B2 JP S5952977B2
- Authority
- JP
- Japan
- Prior art keywords
- standard solution
- turbidity
- present
- turbidity standard
- specific gravity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4785—Standardising light scatter apparatus; Standards therefor
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
【発明の詳細な説明】
本発明は長期安定で使用性に優れた比重定量用濁度標準
液に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a turbidity standard solution for determining specific gravity that is stable over a long period of time and has excellent usability.
更に詳しくは、本発明は臨床検査の分野で肝機能検査法
として用いられるチモール混濁反応（ＴＴＴ）、硫酸亜
鉛混濁反応（ＺＴＴ）等の血清膠質反応用、あるいは細
菌の増殖菌数の判定用、または混濁度イムノアツセイ（
ＴＩＡ）用の安定性の高い比重定量用濁度標準液の製法
に関するものである。More specifically, the present invention is applicable to serum colloid reactions such as thymol turbidity reaction (TTT) and zinc sulfate turbidity reaction (ZTT) used as liver function testing methods in the field of clinical testing, or for determining the number of proliferating bacteria; or turbidity immunoassay (
The present invention relates to a method for producing a highly stable turbidity standard solution for specific gravity determination for TIA).
一般に混濁状況は比色定量あるいは肉眼判定で測定され
ており、本発明はこれら両測定法の対照としての標準液
を提供することを目的とするものである。Generally, turbidity is measured by colorimetry or visual judgment, and the object of the present invention is to provide a standard solution as a reference for both of these measurement methods.
従来、この種の濁度標準液として塩化バリウム−硫酸系
の濁りを標準とするものやコロイド状ガラスによる濁り
を標準とするもの等があるが、前者は安定性が悪いため
使用の度に製造しなければならず、混濁度の一定のもの
を製造するためにはフ温度、反応速度、攪拌条件等の反
応条件を厳密に行なう必要があり、製造技術的に難しい
。Conventionally, this type of turbidity standard solution includes one based on barium chloride-sulfuric acid based turbidity and one based on colloidal glass turbidity, but the former has poor stability and has to be manufactured each time it is used. In order to produce a product with a constant turbidity, it is necessary to strictly control reaction conditions such as temperature, reaction rate, and stirring conditions, which is difficult from a manufacturing technology standpoint.
また後者は沈澱速度が大きいため比重の度ごとに充分に
振盪しなければならない。また両者共に測定における再
現性は２０％〜３０％の大きなバラツキを生じ・る等の
欠点がある。安定性の高い濁度標準液として特願昭４４
一１７５４９号（特公昭４９−３１１５６号）にポリス
チレンラテックスを３０〜５０％アルコール溶液にて懸
濁して製造する方法があるが、この標準液も製造後、ラ
ッテックス粒子が沈降し、使用の度、振盪しなければな
らず、又保存期間も１年位までであつた。Furthermore, since the latter has a high sedimentation rate, it must be thoroughly shaken every time the specific gravity is adjusted. Furthermore, both methods have drawbacks such as large variations in reproducibility of measurements of 20% to 30%. Patent application filed in 1974 as a highly stable turbidity standard solution
No. 117549 (Japanese Patent Publication No. 49-31156) discloses a method of manufacturing polystyrene latex by suspending it in a 30 to 50% alcohol solution, but this standard solution also causes latex particles to settle after manufacture, and each time it is used, It had to be shaken, and its shelf life was only about one year.
理想的な標準液としては１ 経時変化が小さく長期間安
定なもの。The ideal standard solution is one that shows little change over time and is stable for a long period of time.
２ 振盪操作の不要なもの。2 Items that do not require shaking operations.
３ 濁度状況が被検液に類似しているもの。3 The turbidity is similar to the test liquid.
□ 室温保存で安定なもの。□ Stable when stored at room temperature.
５ 製造が容易なもの。5. Something that is easy to manufacture.
以上の条件を満たすものが望ましい。It is desirable that the above conditions be met.
本発明は光学的特性を高めるために粒子径０．５フμ〜
２．０μの範囲で粒子径の異なる１種以上のラテックス
粒子を用いることにより濁度状況を被検体の濁度状況に
類似させ、比重調整剤を加え比重を０．８〜１．３とす
ることにより振盪操作を不要とし、更に界面活性剤を加
えることにより安定性を向上させ、上記の条件をすべて
ほぼ満足させる濁度標準液を提供するものである。In the present invention, in order to improve optical properties, the particle size is 0.5 μm or more.
By using one or more types of latex particles with different particle diameters in the range of 2.0μ, the turbidity situation is made similar to that of the specimen, and a specific gravity adjuster is added to adjust the specific gravity to 0.8 to 1.3. This provides a turbidity standard solution that eliminates the need for a shaking operation, improves stability by adding a surfactant, and substantially satisfies all of the above conditions.
本発明に用いられるラテックスとしてはスチレン、もし
くはその誘導体、オレフイン、アクリル酸等のラテツク
スがある。The latex used in the present invention includes latexes of styrene or its derivatives, olefin, acrylic acid, and the like.
また比重調整剤として用いられる糖類としては白糖、果
糖、ブドウ糖等種々のものが使用可能であり、ガム類と
してはアラビアガム等、セルロース類としてはメチルセ
ルロース、カノレボキシメチノレセノレロース、グリコ
ール類としてはグリコール、エチレングリコール等が用
いられる。また、界面活性剤としては公知のものが使用
できるが特に非イオン性界面活性剤が好ましい。In addition, various sugars such as white sugar, fructose, and glucose can be used as specific gravity adjusting agents, gums such as gum arabic, celluloses such as methylcellulose, canoleboxymethinoresenolose, and glycols. Glycol, ethylene glycol, etc. are used. Further, as the surfactant, known surfactants can be used, but nonionic surfactants are particularly preferred.
本発明の濁度標準液を製するにあたり、その液性は中性
もしくはアルカリ性側が好ましく、適応する緩衝液、例
えばトリス緩衝液等でＰＨを調整することが安定性にお
いて更に有利である。濁度標準液の濁度単位としては前
述の塩化バリウム一硫酸系の硫酸バリウム標準液列によ
るマグファーラント番号（ＭｃＦａｒｌａｎｄＮＯ．Ｍ
ｃＦａｒｌａｎｄ．Ｊ．：Ｊ．Ａ．Ｍ．Ａ．、４９、１
１７８、１９０７．）が使用されている。When producing the turbidity standard solution of the present invention, it is preferable that the liquid property is neutral or alkaline, and it is more advantageous in terms of stability to adjust the pH with an appropriate buffer solution, such as Tris buffer. The turbidity unit of the turbidity standard solution is the McFarland NO.
cFarland. J. :J. A. M. A. ,49,1
178, 1907. ) is used.
本発明による濁度標準液の単位決定においても長波長に
おける吸光度によつて適当なマタフアーランド番号に調
製し処方決定した。次に本発明を以下の実施例により説
明するが、これによつて本発明の範囲が限定されるもの
ではない。In determining the unit of the turbidity standard solution according to the present invention, the formulation was determined by preparing an appropriate material furand number based on the absorbance at long wavelengths. Next, the present invention will be explained with reference to the following examples, but the scope of the present invention is not limited thereby.
実施例
０．０９／Ｌトリス緩衝液（ＰＨ９．Ｏ）７９９ｍ１中
にエチレングリコール２００ｍ１とトリトンＸ−１００
（口ーム・アンド・バース社製）１．０ｍ１とを加え混
和し希釈液とする。Example 200 ml of ethylene glycol and Triton X-100 in 799 ml of 0.09/L Tris buffer (PH 9.0)
(manufactured by Mouth & Birth) and mix to obtain a diluted solution.
粒子径０．８０４μのポリスチレンラテツクス粒子１０
％懸濁液の０．１ｍ１と粒子径２．０２μのポリスチレ
ンラテツクス粒子１０％懸濁液の０．２６７ｍ１を上記
の希釈液中に加え混和し標準液とする。この濁度標準液
はマグファーラント番号４に相当する濁度単位を示した
。10 polystyrene latex particles with a particle size of 0.804μ
% suspension and 0.267 ml of a 10% suspension of polystyrene latex particles with a particle size of 2.02μ are added to the above diluted solution and mixed to form a standard solution. This turbidity standard solution showed a turbidity unit corresponding to Magfurant number 4.
他のマグファーラント番号に相当する標準液を得るには
前記の希釈液の増量または減量により調製することがで
きる。参考例 １
従来の塩化バリウム一硫酸系による標準液（以下硫酸バ
リウム標準液という）と本発明による標準液との濁度の
再現性を比較するために日立１０１型分光々度計を使用
し波長６６０ｎｍにおける吸光度を測定した。Standard solutions corresponding to other Magfurant numbers can be prepared by increasing or decreasing the amount of the diluent described above. Reference Example 1 In order to compare the reproducibility of turbidity between a conventional barium chloride monosulfate-based standard solution (hereinafter referred to as barium sulfate standard solution) and a standard solution according to the present invention, a Hitachi Model 101 spectrophotometer was used to determine the wavelength. Absorbance was measured at 660 nm.
両者共にマグファーラント番号１、２、および３の３種
の濁度単位のものを各々５回ずつ測定した。第１図に結
果を表示する。この結果より各マグファーラント番号共
に硫酸バリウム標準液は著しくバラツキ、本発明による
濁度標準液の再現性は優れていることが明らかである。
図中、Ｘで示したものは硫酸バリウム標準液、○で示し
たものは本発明による標準液を表わす。参考例 ２
本発明による標準液の安定性を示すために調製直後、１
年経過後、２年経過後、３年経過後のものについて、そ
れぞれ日立１２４型分光々度計を使用し波長６６０ｎｍ
における吸光度を測定した。In both cases, three types of turbidity units with magfurant numbers 1, 2, and 3 were measured five times each. The results are shown in Figure 1. From these results, it is clear that the barium sulfate standard solutions vary significantly for each Magfurant number, and that the reproducibility of the turbidity standard solution according to the present invention is excellent.
In the figure, those indicated by X represent barium sulfate standard solutions, and those indicated by O represent standard solutions according to the present invention. Reference Example 2 In order to demonstrate the stability of the standard solution according to the present invention, immediately after preparation, 1
After 1 year, 2 years, and 3 years, a Hitachi 124 type spectrophotometer was used to measure the wavelength of 660 nm.
The absorbance at was measured.
その結果を第２図に表示する。この結果より本発明によ
る濁度標準液は３年間経過後も調製直後とほとんど変化
なく、安定していることが明らかである。The results are shown in Figure 2. From these results, it is clear that the turbidity standard solution according to the present invention remains stable even after three years with almost no change from immediately after preparation.
第１図は硫酸バリウム標準液と本発明による標準液との
マグファーラント番号１、２、および３の３種の濁度単
位のものを各々５回ずつ、日立１０１型分光々度計で波
長６６０ｎｍにおける吸光度を測定した結果を示す。
図中、○は本発明による標準液、Ｘは硫酸バリウム標準
液を表わす。
第２図は本発明による標準液について調製直後、１年経
過後、２年経過後、３年経過後のものについて、それぞ
れ日立１２４型分光々度計で波長６６０ｎｍにおける吸
光度を測定した結果を示す。Figure 1 shows how the barium sulfate standard solution and the standard solution according to the present invention were measured in three turbidity units with magfarant numbers 1, 2, and 3 five times each using a Hitachi Model 101 spectrophotometer. The results of measuring absorbance at 660 nm are shown. In the figure, ○ represents the standard solution according to the present invention, and X represents the barium sulfate standard solution. Figure 2 shows the results of measuring the absorbance at a wavelength of 660 nm using a Hitachi Model 124 spectrophotometer for the standard solution according to the present invention immediately after preparation, after 1 year, after 2 years, and after 3 years, respectively. .
Claims (1)
１種以上のラテックス粒子と比重調整剤および界面活性
剤を含有することを特徴とする比濁定量用濁度標準液の
製法。 ２ 比重調整剤が糖類、ガム類、セルロース類またはグ
リコール類の内の１種、もしくは１種以上から成ること
を特徴とする特許請求の範囲第１項記載の比濁定量用濁
度標準液の製法。 ３ 比重を０．８〜１．３の範囲とすることを特徴とす
る特許請求の範囲第１項記載の比濁定量用濁度標準液の
製法。[Scope of Claims] 1. A nephelometric turbidity characterized by containing one or more latex particles having different particle diameters in the range of 0.5 μm to 2.0 μm, a specific gravity adjuster, and a surfactant. Manufacturing method of standard solution. 2. The turbidity standard solution for nephelometric determination according to claim 1, wherein the specific gravity adjusting agent is composed of one or more of sugars, gums, celluloses, or glycols. Manufacturing method. 3. The method for producing a turbidity standard solution for nephelometric determination according to claim 1, characterized in that the specific gravity is in the range of 0.8 to 1.3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9167179A JPS5952977B2 (en) | 1979-07-20 | 1979-07-20 | Production method of turbidity standard solution for nephelometric determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9167179A JPS5952977B2 (en) | 1979-07-20 | 1979-07-20 | Production method of turbidity standard solution for nephelometric determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5616845A JPS5616845A (en) | 1981-02-18 |
| JPS5952977B2 true JPS5952977B2 (en) | 1984-12-22 |
Family
ID=14032937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9167179A Expired JPS5952977B2 (en) | 1979-07-20 | 1979-07-20 | Production method of turbidity standard solution for nephelometric determination |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5952977B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK164144C (en) * | 1988-11-17 | 1992-10-12 | Slagteriernes Forskningsinst | PROCEDURE FOR CONTROL OR ADJUSTMENT OF MEASURING APPLIANCES WITH AN OPTICAL SOUND AND MEDIUM USED BY THE PROCEDURE |
| US20060045891A1 (en) * | 2004-08-24 | 2006-03-02 | Lovalenti Phillip M | Density-matched suspension vehicles and pharmaceutical suspensions |
| JP4946047B2 (en) * | 2005-12-28 | 2012-06-06 | 東洋紡績株式会社 | Particle standard reagent for formed component classifier |
| CN108761070B (en) * | 2018-05-03 | 2021-04-02 | 柏荣诊断产品（上海）有限公司 | Urine transferrin detect reagent box of wide detection range |
-
1979
- 1979-07-20 JP JP9167179A patent/JPS5952977B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5616845A (en) | 1981-02-18 |
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